Holm Oak (Quercus ilex subsp. ballota (Desf.) Samp.) Bark Aqueous Ammonia Extract for the Control of Invasive Forest Pathogens.

Holm oak (Quercus ilex subsp. ballota (Desf.) Samp.) bark is a commonly used remedy to treat gastrointestinal disorders, throat and skin infections, hemorrhages, and dysentery. It has also been previously reported that its methanol extracts possess antibacterial activity, which can be related to the richness of Quercus spp. extracts in phenolic compounds, such as flavonoids and tannins. However, there is no information on the antifungal (including oomycete) properties of the bark from Q. ilex or its subspecies (ilex and ballota). In this work, we report the characterization of the aqueous ammonia extract of its bark by FTIR and GC-MS and the results of in vitro and ex situ inhibition tests against three phytopathogens. The main phytochemical components identified were inositols (19.5%), trans-squalene (13%), 4-butoxy-1-butanol (11.4%), gulopyranose (9.6%), lyxose (6.5%), 2,4-dimethyl-benzo[H]quinoline (5.1%), catechol (4.5%), and methoxyphenols (4.2%). The efficacy of the extract in controlling forest phytopathogens was tested in vitro against Fusarium circinatum (responsible for pitch canker of Pinus spp.), Cryphonectria parasitica (which causes chestnut blight), and Phytophthora cinnamomi (which causes 'root and crown rot' in a variety of hosts, including Castanea, conifers, Eucalyptus, Fagus, Juglans, Quercus, etc.), obtaining EC90 values of 322, 295, and 75 µg·mL-1, respectively, much lower than those attained for a commercial strobilurin fungicide (azoxystrobin). The extract was further tested ex situ against P. cinnamomi on artificially inoculated, excised stems of 'Garnem' almond rootstock, attaining complete protection at a dose of 782 µg·mL-1. The results suggest that holm oak bark extract may be a promising source of bioactive compounds against invasive forest pathogens, including the oomycete that is causing its decline, the so-called 'seca' in Spain.

On the Physicochemical Characteristics and Applications of an "Undesirable" Pyrenean Thorny Cushion Dwarf: Echinospartum horridum (Vahl) Roth.

Small evergreen shrubs of the family Fabaceae represent a large proportion of current Mediterranean mountain vegetation. Their low pastoral value and tendency for encroachment makes these plants undesirable. In this paper, the thermal and chemical characteristics of Echinospartum horridum, a thorny cushion-shaped dwarf shrub native to the French Central Massif and the Pyrenees (particularly dominant in the shrublands of the Pyrenees), have been analyzed with a view to its valorization. Although the higher and lower heating values of the biomass from E. horridum met the ISO 17225-2:2014 requirements for its use in pellets, the ash content was slightly above the upper limit, so it would not comply with the normative for its acceptable use as a fuel. Nevertheless, the presence of high added-value flavonoids and lignans in its extracts, which are receiving increasing recent interest as efficient anti-tumor drugs and antivirals, may open the door to the valorization of this shrub for pharmacological applications.