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Artículo en Chino | MEDLINE | ID: mdl-18441904

RESUMEN

A modified dye test with microplate was to be established to detect Toxoplasma antibodies with cell-cultured Toxoplasma gondii. Numbers of stained and unstained tachyzoites were estimated in every 100 tachyzoites in each well after dyeing with methylene blue. The dilution with 50% tachyzoites stained was used as final dilution. Better results of the microplate dye test has been received when the concentration of tachyzoites in suspension reaches 10(9)/ml with 1% sodium citrate as accessory factor.


Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Toxoplasma/inmunología , Toxoplasmosis/diagnóstico , Animales , Humanos , Inmunoglobulina A/sangre , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Pruebas de Neutralización , Sensibilidad y Especificidad , Coloración y Etiquetado/métodos , Toxoplasmosis/sangre , Toxoplasmosis/parasitología
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