Generation and phenotypic characterization of S100A9 gene knockout mice by CRISPR/Cas9-mediated gene targeting / 生理学报

S100 calcium binding protein A9 (S100A9) is involved in a variety of biological processes such as inflammation and tumor cell migration and invasion regulation. The purpose of this study was to construct S100A9 gene-edited mice by using CRISPR/Cas9 technology, thereby providing an animal model for exploring the biological functions of this gene. According to the S100A9 gene sequence, the single-stranded small guide RNA (sgRNA) targeting exons 2 and 3 was transcribed in vitro, and a mixture of Cas9 mRNA and candidate sgRNA was injected into mouse fertilized eggs by microinjection. Early embryos were obtained and transferred to surrogate mice, and F

Mechanism of apoptosis induced by ethyl acetate extract from Polygonum amplexicaule var. sinense in lung cancer cells / 中草药

To study the influence of ethyl-acetate extract of Polygonum amplexicaule var. sinense (EAEP) on lung cancer cellular proliferation and apoptosis and the underlying mechanisms. Methods The cell growth of A549 and H1299 cells was measured by cell counting kit-8 experiment and apoptosis was analyzed by flow cytometry. The treated cellular total mRNAs and proteins related to the apoptosis of A549 and H1299 cells were extracted, and then used for qRT-PCR and Western blotting experiments. Results The apoptosis rate of A549 and H1299 cells was observed to be markedly promoted by EAEP, and flow cytometry statistics suggested that this effect may mainly be produced by its pro-apoptotic procedures. qRT-qPCR and Western blotting assays also proved that EAEP significantly promoted expression of Bax and Caspase-3, and inhibited expression of Bcl-2. Conclusion EAEP may contain components that play a role in regulating cellular growth via influencing Bax and Bcl-2 pathway.