Evaluation of physical euthanasia for neonatal piglets on-farm.

Twenty-seven neonate piglets (range from 0.35 to 1.17 kg) were evaluated for the effectiveness of blunt force trauma as a method of on-farm cull. Brainstem function, brain injury, and hemorrhage scores (increasing from 0 to 3) were assessed after striking the head against a concrete floor. Electroencephalograms (EEG) from a subset of 15 piglets were recorded before and after blunt force trauma for electrophysiological assessments. Blunt force trauma was performed by a single experienced farmer in a commercial farm by holding the piglet by its both hind legs and striking the head against the concrete floor. All piglets remained recumbent and did not show brainstem reflexes. Only one piglet did not presented tonic/clonic physical activity. The mean time to the onset of persistent isoelectric EEG was 64.3 ± 7.3 s (range 18 to 115). Total power, theta, alpha, and beta power decreased to approximately 45%, 30%, 20%, and 15% from pretreatment power, respectively, by 15-s post-impact. There were no periods of normal-like EEG after the culling. Bruises in the neck and shoulder were found in 67% and 70% of piglets, respectively. All piglets presented skull fractures with 20% having the nasal bone(s) fractured. Brain damage was found in all piglets, mainly in the frontal lobe(s). The occipital lobe(s) presented the greatest frequency of severe damage. The analysis of the radiographs also found a high frequency of fractures in this region. Hemorrhage was most frequent in the frontal, parietal, occipital lobes, and midbrain. When performed correctly with the appropriate weight class, blunt force trauma can be used as an effective method for the on-farm killing of nursing piglets resulting in death. However, this method should not be promoted over more reliable and repeatable cull methods such as captive bolt gun. As with blunt force trauma, there is a significant potential for animal welfare harm associated with inappropriate practice, lack of accuracy, issues with repeatability, and operator fatigue.

Ease of Handling and Physiological Parameters of Stress, Carcasses, and Pork Quality of Pigs Handled in Different Group Sizes.

The effect of different group sizes of pigs (3, 5, and 10 pigs) during handling on physiological parameters, carcasses, and pork quality traits at the farm and slaughterhouse were evaluated in 360 pigs from five farms (four repetitions or group/treatment/farms). Data was analyzed as a factorial of 3 × 5 (3 treatments × 5 farms) to check effects of treatments by analysis of variance in ANOVA. Ease of handling decreased as the group size increased. However, time taken in handling was not influenced by the group size (p > 0.10). Moving pigs in groups of five animals reduced effects on blood cortisol levels (p < 0.05). Fighting and handling lesions in the carcasses increased for bigger handling groups (p < 0.05). Pigs handled in groups of three and ten animals had a higher pHu and initial temperature in Longissimus thoracis and Semimembranosus (p < 0.05) and lower drip loss in Semimembranosus (p < 0.05). However, meat quality classifications of the carcasses were not affected by treatments. Based on the results, moving groups of five pigs seems to be the best strategy to improve animal welfare, carcasses and pork quality.

Effects of shade location and protection from direct solar radiation on the behavior of Holstein cows.

Two trials (E1 and E2) were performed to assess the behavior of eight Holstein dairy cows with 367 ± 58 kg of body weight and 10.52 ± 0.08 kg of milk yield. A 4 × 4 Latin square design (four periods of lactation and four levels of solar blockage) with four paddocks was used. Each paddock contained a wood shading structure covered with a cloth that blocked 30% (T1), 50% (T2), 70% (T3), or 100% (T4) of direct solar radiation. In the first trial (E1) each shade structure was located approximately 40 m from the feeder and water troughs; in the second trial (E2), the distance was reduced to 5 m. Air temperature (TA, °C), relative humidity (RH, %), wind speed (U, ms-1), black globe temperature (TG, K), mean radiant temperature (TMR, K), radiant heat load (RHL, W m-2), and local shortwave radiation (RS, W m-2) were recorded at 15-min intervals from 08:00 to 17:00 h. Four behavioral activities were recorded: grazing, eating at the feed trough, ruminating, and idling. For each of these activities, animal posture (lying or upright) and location (under shade or exposed to sunlight) were recorded. The meteorological conditions showed similar variations from 8:00 to 17:00 h between the two trials. However, the air temperatures in E1 were lower (± 2 °C) than those in E2. In a PCA analysis, the first and the second principal components explained 56.87% and 21.85%, respectively, of the total variation in the behavioral variables. Under the E1 conditions, the animals did not seek shade, whereas in E2, the dairy cows spent 35 ± 5% of their time lying and idling in the shade. At a solar radiation blockage of 100%, cows were in the shade more than 60% of the time due to the intensity of solar radiation, which was 722.19 ± 14.59 W m-2 at 11:45. In a PCA analysis, the first and the second principal components explained 65.18 and 22.3%, respectively, and 87.48% together, of the total variation in the original variables. Consequently, it was possible to develop a shade index (IST) based on the first two components. In E1, animals spent very little time in the shade, spending only 0.15% of total time under the shade, irrespective of blockage. However, E2 cows used shade, reaching almost 80% of time under the shade, at midday, when the blockage was 100%.

Risk factors associated with pig pre-slaughtering losses.

The incidence of non-ambulatory non-injured (NANI), non-ambulatory injured (NAI) and dead pigs on-arrival at three Brazilian slaughterhouses were evaluated in 37,962 pigs to identify risk factors linked to them, besides carcass bruises and limb fractures. Total pre-slaughtering losses were 1.18%, in which NAI (0.39%) and NANI (0.37%) incidences contributed the most. A positive relation between on farm steeper ramp slope >20° and the incidence of NAI, NANI and dead pigs at unloading was found. Farm size, pigs/pen, enthalpy at loading, transportation time, truck loading order, muscle thickness and carcass weight, were identified as risk factors for pre-slaughtering losses. Loading procedures influenced the occurrence of limb fractures and bruises (which are a welfare issue and should be reduced). Therefore, personal training on pre-slaughter handling is essential to reduce the risk factors to improve animal welfare and avoid losses during the pre-slaughter process.

How do season, on-farm fasting interval and lairage period affect swine welfare, carcass and meat quality traits?

This study was carried out in order to determine the effects of different combinations of on-farm fasting intervals (8, 12, 16, 20 h) and 1.5 h of transport plus lairage periods (1, 3, 6 h) at different seasons (summer/winter) on blood stress parameters (cortisol and lactate), stomach content and weight, skin lesion and meat quality in a total of 960 pigs from eight farms. Blood lactate levels were greater in the summer (P < 0.001) and stomach content was affected (P < 0.05) by season, on-farm fasting interval (P < 0.001), lairage time (P < 0.0001). Stomach content weight reduces as the total feed withdrawal time increases up to on-farm fasting of 17 and 1 h of lairage. Stomach content can be influenced by feed and water in different ways according to treatments. Only 8 h of on-farm fasting is not enough to empty stomachs from feed content. However, an on-farm fasting period of 16 h or longer can also increase the occurrence of more water in the stomachs. Carcass lesions caused by fighting were greater (P ≤ 0.005) in the winter, mainly after 3 and 6 h of lairage (P ≤ 0.005). Loin and ham pHu was lower (P ≤ 0.05) for pigs slaughtered after 6 h of lairage during the summer. The application of 12 h of on-farm fasting with 6 h of lairage seemed to be best combination to reduce stomach content weight (feed and water). In the winter, shorter lairage period can be used to reduce percentage of skin lesions and better pork quality traits in pigs.

Effects of the Truck Suspension System on Animal Welfare, Carcass and Meat Quality Traits in Pigs.

The objective of this study was to assess the effects of two types of commercial suspension (leaf-spring (LS) vs. air suspension (AS)) installed on two similar double-decked trucks on blood cortisol and lactate concentration, lairage behavior, carcass skin lesions and pork quality traits of 120 crossbred pigs. The suspension type neither influenced pig behaviour in lairage nor blood cortisol and lactate concentrations (p > 0.10). However, when compared with the AS suspension system, the use of LS increased the number of skin lesions in the back and thigh (p = 0.03 and p = 0.01, respectively) and produced thigh with lower pHu (p < 0.001) and yellower colour (higher b* value; p = 0.03), and paler back muscles (subjective colour; p < 0.05), with a tendency to lower pH (p = 0.06). Therefore, the use air suspension system can improve carcass and meat quality traits of pigs transported to slaughter.