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1.
Lett Appl Microbiol ; 30(4): 287-93, 2000 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-10792648

RESUMEN

Two DNA fragments from Xanthomonas albilineans were used as probes to study the molecular diversity among strains of this pathogen. Two serologically distinct groups, serovars I and II, could be differentiated by hybridization to the probes. These probes, designated 830 and 838, were cloned after subtractive DNA hybridization of common sequences of Xanthomonas campestris pv. vasculorum from a serovar I strain of X. albilineans. They did not hybridize to the DNA of several other xanthomonads or to sugarcane DNA under the conditions of hybridization used. Faint bands were observed upon hybridization of probe 830 with one strain of X. campestris pv. phaseoli. The same banding patterns were obtained with a strain of X. albilineans from Burkina Faso and the serovar II strains of Mauritius. The serovar I strains from Mauritius and two other strains each from Reunion and South Africa had similar pattern.


Asunto(s)
Enfermedades de las Plantas/microbiología , Polimorfismo Genético , Xanthomonas/clasificación , Xanthomonas/genética , África , Australia , Southern Blotting , Brasil , Sondas de ADN/genética , ADN Bacteriano/análisis , ADN Bacteriano/genética , Islas del Oceano Índico , Hibridación de Ácido Nucleico/métodos , Papúa Nueva Guinea , Especificidad de la Especie , Estados Unidos , Xanthomonas/aislamiento & purificación
2.
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