Presença de Leishmania sp. e Dirofilaria immitis em / Tumor Venéreo Transmissível Canino cutâneo / Presence of Leishmania sp. and Dirofilaria immitis in Cutaneous Canine Transmissible Venereal Tumor

Background: Canine Transmissible Venereal Tumor (CTVT) is a neoplasm transmitted by implantation of its cells into genitaland extragenital organs, while Heartworm and Visceral Leishmaniasis are zoonosis transmitted by hematophagous insectsthat are often underdiagnosed in asymptomatic animals. Coinfection by the agents of these parasitosis is well documented,however, the association of both diseases with the CTVT is still unusual. Thus, it was aimed to report a case of incidentalidentification of microfilariae of D. immitis and amastigotes forms of Leishmania sp. in cutaneous CTVT by cytology in anasymptomatic dog for the parasitosis.Case: A 6-year-old, male, mongrel, sexually intact dog from the city of Patos, Paraiba, Brazil, was presented with a cutaneouscircumscribed tumoral lesion in a region adjacent to the right olecranon, with an ulcerated surface of reddish color, havingareas of necrosis inside and larvae (myiasis), draining bloody secretion. The material was collected for cytological analysis,which proved to be Canine Transmissible Venereal Tumor (CTVT) with the presence of amastigote forms of Leishmania sp.and microfilariae of D. immitis among neoplastic cells. Blood count, serum urea, creatinine and albumin, ALT, AST, FA,CK, Na+, K+, Ca++, CK-MB, Troponin I (cTnI), Snap 4Dx Plus (Idexx®), Snap Leishmania (Idexx®), and Knotts test wereperformed, plus chest radiography, blood pressure measurements and electrocardiogram (ECG). The alterations found corresponded to normochromic normocytic anemia, thrombocytopenia, hypoalbuminemia, microfilariae in the Knott test, increaseof CK, CK-MB and cTnI, positivity for Erlichia sp., Anaplasma sp., Leishmania sp. and Dirofilaria immitis. Furthermorecardiomegaly on radiographs and sinus arrhythmia associated with atrioventricular block (AVB) of the first degree on theECG. Euthanasia was performed after necropsy, in which adult worms were...(AU)

Presença de Leishmania sp. e Dirofilaria immitis em / Tumor Venéreo Transmissível Canino cutâneo / Presence of Leishmania sp. and Dirofilaria immitis in Cutaneous Canine Transmissible Venereal Tumor

Background: Canine Transmissible Venereal Tumor (CTVT) is a neoplasm transmitted by implantation of its cells into genitaland extragenital organs, while Heartworm and Visceral Leishmaniasis are zoonosis transmitted by hematophagous insectsthat are often underdiagnosed in asymptomatic animals. Coinfection by the agents of these parasitosis is well documented,however, the association of both diseases with the CTVT is still unusual. Thus, it was aimed to report a case of incidentalidentification of microfilariae of D. immitis and amastigotes forms of Leishmania sp. in cutaneous CTVT by cytology in anasymptomatic dog for the parasitosis.Case: A 6-year-old, male, mongrel, sexually intact dog from the city of Patos, Paraiba, Brazil, was presented with a cutaneouscircumscribed tumoral lesion in a region adjacent to the right olecranon, with an ulcerated surface of reddish color, havingareas of necrosis inside and larvae (myiasis), draining bloody secretion. The material was collected for cytological analysis,which proved to be Canine Transmissible Venereal Tumor (CTVT) with the presence of amastigote forms of Leishmania sp.and microfilariae of D. immitis among neoplastic cells. Blood count, serum urea, creatinine and albumin, ALT, AST, FA,CK, Na+, K+, Ca++, CK-MB, Troponin I (cTnI), Snap 4Dx Plus (Idexx®), Snap Leishmania (Idexx®), and Knott’s test wereperformed, plus chest radiography, blood pressure measurements and electrocardiogram (ECG). The alterations found corresponded to normochromic normocytic anemia, thrombocytopenia, hypoalbuminemia, microfilariae in the Knott test, increaseof CK, CK-MB and cTnI, positivity for Erlichia sp., Anaplasma sp., Leishmania sp. and Dirofilaria immitis. Furthermorecardiomegaly on radiographs and sinus arrhythmia associated with atrioventricular block (AVB) of the first degree on theECG. Euthanasia was performed after necropsy, in which adult worms were...

Clinical effectiveness of autohemotherapy as an adjunct treatment of canine parvovirus

Background: Canine parvovirus is highlighted among the gastroenteric diseases that are common medical occurrences in small animals due to its clinical evolution. Although the treatment is often successful, the success rate has remained unchanged over the years, reflecting a clear need for searching more efficient protocols in order to reduce hospitalization, treatment cost and increase survival rates. Autohemotherapy efficacy has been demonstrated for several diseases and identified as a stimulating tool and immune mediator. Thus, the objective of this research was to determine the clinical effectiveness of autohemotherapy as an adjunct in the treatment of dogs suffering from parvovirus. Materials, Methods & Results: Twenty dogs suffering from parvovirus, diagnosed via immunochromatography, were distributed (n = 10) into two groups: autohemotherapy (GAHT) and control (GCO). Both groups were treated with the appropriate support therapy for hemorrhagic gastroenteritis (GHE), while the dogs of the GAHT group were also treated with the adjunct autohemotherapy (AHT). The AHT consisted of the administration of blood collected by jugular venipuncture, without anticoagulant, immediately injected in equal proportion in the semitendinosus and semimembranosus muscle region. The blood volume injected was based on animal weight:...

Clinical effectiveness of autohemotherapy as an adjunct treatment of canine parvovirus

Background: Canine parvovirus is highlighted among the gastroenteric diseases that are common medical occurrences in small animals due to its clinical evolution. Although the treatment is often successful, the success rate has remained unchanged over the years, reflecting a clear need for searching more efficient protocols in order to reduce hospitalization, treatment cost and increase survival rates. Autohemotherapy efficacy has been demonstrated for several diseases and identified as a stimulating tool and immune mediator. Thus, the objective of this research was to determine the clinical effectiveness of autohemotherapy as an adjunct in the treatment of dogs suffering from parvovirus. Materials, Methods & Results: Twenty dogs suffering from parvovirus, diagnosed via immunochromatography, were distributed (n = 10) into two groups: autohemotherapy (GAHT) and control (GCO). Both groups were treated with the appropriate support therapy for hemorrhagic gastroenteritis (GHE), while the dogs of the GAHT group were also treated with the adjunct autohemotherapy (AHT). The AHT consisted of the administration of blood collected by jugular venipuncture, without anticoagulant, immediately injected in equal proportion in the semitendinosus and semimembranosus muscle region. The blood volume injected was based on animal weight:...(AU)

Sensibilidade “in vitro” de Bactérias Isoladas das Fezes de CãesAcometidos por Parvovirose / In vitro Antimicrobial Susceptibility of Bacteria Isolated of Feces of Dogs with Parvovirose

Objetivou-se com este estudo evidenciar e avaliar o perfil de sensibilidade e ocorrência de resistênciaantimicrobiana de bactérias isoladas das fezes de cães acometidos por parvovirose. Foram colhidas12 amostras fecais de cães acometidos por gastroenterite hemorrágica por Parvovírus, diagnosticadosatravés do método de imunoensaio cromatográfico. As amostras foram encaminhadas ao Laboratóriode Microbiologia para a realização da identificação bacteriológica e teste de sensibilidadeantimicrobiana. Com base nos resultados obtidos, concluiu-se que cepas de Escherichia coli forampredominantes nos distúrbios gastroentéricos por Parvovírus. No que se refere a sensibilidade antimicrobiana,considerando as cepas de um modo geral, foram observados maiores índices de sensibilidadea amoxicilina + clavulanato, ampicilina e gentamicina, em contrapartida, altos índices deresistência a cefalotina, neomicina e tetraciclina. Desta forma, ressalta-se a importância da realizaçãode análises microbiológicas, bem como o uso racional de antimicrobianos, frente a desordens gastrointestinaisem cães.

The objective of this study was to evidence and to evaluate the sensibility profile and occurrenceof resistance antimicrobial of isolated bacteria of the feces of dogs attacked by parvovirose. Wecollected fecal samples from 12 dogs affected by hemorrhagic gastroenteritis Parvovirus, diagnosedby the method of chromatographic immunoassay. The samples were sent to the microbiology laboratoryto perform bacterial identification and antimicrobial susceptibility testing. Based on theseresults, we conclude that E. coli strains were predominant in the gastrointestinal disturbances byParvovirus in dogs. The antimicrobial sensitivi ty, considering the strains in general, showed higherlevels of sensitivity to amoxicillin + clavulanate, ampicillin and gentamicin, however, highrates of resistance to cephalothin, neomycin and tetracycline. Thus, it emphasizes the importanceof performing microbiological analyzes as well rational use of antibiotics for treatment of gastrointestinaldisorders in dogs.

Sensibilidade “in vitro” de Bactérias Isoladas das Fezes de CãesAcometidos por Parvovirose / In vitro Antimicrobial Susceptibility of Bacteria Isolated of Feces of Dogs with Parvovirose

Objetivou-se com este estudo evidenciar e avaliar o perfil de sensibilidade e ocorrência de resistênciaantimicrobiana de bactérias isoladas das fezes de cães acometidos por parvovirose. Foram colhidas12 amostras fecais de cães acometidos por gastroenterite hemorrágica por Parvovírus, diagnosticadosatravés do método de imunoensaio cromatográfico. As amostras foram encaminhadas ao Laboratóriode Microbiologia para a realização da identificação bacteriológica e teste de sensibilidadeantimicrobiana. Com base nos resultados obtidos, concluiu-se que cepas de Escherichia coli forampredominantes nos distúrbios gastroentéricos por Parvovírus. No que se refere a sensibilidade antimicrobiana,considerando as cepas de um modo geral, foram observados maiores índices de sensibilidadea amoxicilina + clavulanato, ampicilina e gentamicina, em contrapartida, altos índices deresistência a cefalotina, neomicina e tetraciclina. Desta forma, ressalta-se a importância da realizaçãode análises microbiológicas, bem como o uso racional de antimicrobianos, frente a desordens gastrointestinaisem cães.(AU)

The objective of this study was to evidence and to evaluate the sensibility profile and occurrenceof resistance antimicrobial of isolated bacteria of the feces of dogs attacked by parvovirose. Wecollected fecal samples from 12 dogs affected by hemorrhagic gastroenteritis Parvovirus, diagnosedby the method of chromatographic immunoassay. The samples were sent to the microbiology laboratoryto perform bacterial identification and antimicrobial susceptibility testing. Based on theseresults, we conclude that E. coli strains were predominant in the gastrointestinal disturbances byParvovirus in dogs. The antimicrobial sensitivi ty, considering the strains in general, showed higherlevels of sensitivity to amoxicillin + clavulanate, ampicillin and gentamicin, however, highrates of resistance to cephalothin, neomycin and tetracycline. Thus, it emphasizes the importanceof performing microbiological analyzes as well rational use of antibiotics for treatment of gastrointestinaldisorders in dogs.(AU)

Perfil hematológico e bioquímico de cães com gastroenterite hemorrágica por parvovírus diagnosticados pelo método de imunocromatografia / Hematological and biochemical profile of dogs with parvovirus gastroenteritis diagnosed by immunochromatography method

Objetivou-se com a realização deste trabalho determinar as características hematológicas e bioquímicas, de cães acometidos por gastroenterite por Parvovirus, diagnosticados pelo método da imunocromatografia rápida. Foram utilizados cães procedentes de Patos, PB, apresentando sinais de distúrbios gastroentéricos, evidenciados após avaliação clínica geral e laboratorial como quadro gastroentérico infeccioso viral através ensaio imonocromatográfico. O método de imunoensaio cromatográfico empregado neste estudo mostrou-se eficiente no diagnóstico do CPV em cães acometidos por gastroenterite hemorrágica evidenciando de forma precisa o agente primário envolvido. Porém o diagnóstico definitivo não pode ser baseado em apenas um único teste, devendo ser reportado ao profissional Médico Veterinário sua utilização sob aspecto complementar à correlação com outros achados para sua elucidação. Os dados obtidos nesta pesquisa referentes à hematologia e bioquímica sérica apresentaram variações significativas, dignas de serem consideradas frente a achados laboratoriais e suspeita clínica presuntiva de quadros gastroentéricos virais, bem como servirão como valores referenciais para animais acometidos por gastroenterites hemorrágicas, auxiliando clínicos de pequenos animais em sua rotina ambulatorial na elucidação do agente causal.

The objective of this work was to determine the hematological and biochemical characteristics of dogs with parvovirus gastroenteritis diagnosed by rapid immunochromatgraphy method. It was used dogs from Patos municipality, SP, Brazil, showing clinical signs of a gastrointestinal disorder, evident after clinical and laboratory admitted as a general framework gastrointestinal viral infection through testing immunochromatography. The method of chromatographic immunoassay used in this study proved effective in the diagnosis of CPV in dogs affected by hemorrhagic gastroenteritis showing the primary agent involved. However, the diagnosis cannot be based on just a single test, should be reported to the professional veterinarian complementary aspect of its use in correlation with other findings for their elucidation. Data from this survey relating to hematology and serum biochemistry showed significant variations, worthy of considerations before the laboratory finding sand clinical suspicion of presumptive viral gastrointestinal frames, as well as serve as reference values for animals affected by hemorrhagic gastroenteritis, helping small animal clinical in their routine outpatient clinic in the elucidation of the causative agent.