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Coronavirus disease 2019 (Covid-19) disease have been associated with significant mortality amongst doctors globally including Bangladesh. To delineate the clinico-epidemiological characteristics of the physician affected with Covid-19 was the objective of the study. This cross-sectional 'Facebook' based survey was conducted in the period of August 2020 to September 2020. Snowball sampling methods was followed. A total of 151 physicians affected with Covid-19 participated in this survey. Self-reported perceived severity scale (zero meaning not severe at all and ten denoting the most severe) was used. Collected data were analyzed by SPSS 25.0. Among the participants, the majority were male, 98(64.9%). The most prevalent affected age groups were 24-35 years 131(86.8%). Approximately 45.0% worked in COVID dedicated hospital. Entry-level physicians (Medical Officer or Assistant Surgeon) were the most affected 117(94.4%). One-third of the physicians had at least the one co-morbidity. Bronchial asthma, obesity and diabetes were the most frequent. Predominate symptoms of the infection were fever 94(62.3%), cough 94(62.3%) and myalgia 92(60.9%). Half of the participants had sore throat, anosmia, gastro-intestinal symptoms and one-third of the patients developed dyspnea. Perceived severity of the symptoms ranged between 2 and 6. The pattern of drug use to prevent the Covid-19 showed no uniformity. However, intake of Zinc, Vitamin C, Vitamin D, antihistamine and Ivermectin was found in 74.8%, 67.5%, 41.7%, 49.0% and 37.7% respectively. As the current pandemic continues to evolve, physicians must be equipped with appropriate knowledge, skills and must be cautious on the prevention measures against Covid-19.
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COVID-19 , Humanos , COVID-19/epidemiología , Masculino , Femenino , Estudios Transversales , Adulto , Bangladesh/epidemiología , Médicos/estadística & datos numéricos , Persona de Mediana Edad , SARS-CoV-2 , Adulto Joven , Comorbilidad , Índice de Severidad de la Enfermedad , Encuestas y CuestionariosRESUMEN
Radon (222Rn), an inert gas, is considered a silent killer due to its carcinogenic characteristics. Dhaka city is situated on the banks of the Buriganga River, which is regarded as the lifeline of Dhaka city because it serves as a significant source of the city's water supply for domestic and industrial purposes. Thirty water samples (10 tap water from Dhaka city and 20 surface samples from the Buriganga River) were collected and analyzed using a RAD H2O accessory for 222Rn concentration. The average 222Rn concentration in tap and river water was 1.54 ± 0.38 Bq/L and 0.68 ± 0.29 Bq/L, respectively. All the values were found below the maximum contamination limit (MCL) of 11.1 Bq/L set by the USEPA, the WHO-recommended safe limit of 100 Bq/L, and the UNSCEAR suggested range of 4-40 Bq/L. The mean values of the total annual effective doses due to inhalation and ingestion were calculated to be 9.77 µSv/y and 4.29 µSv/y for tap water and river water, respectively. Although all these values were well below the permissible limit of 100 µSv/y proposed by WHO, they cannot be neglected because of the hazardous nature of 222Rn, especially considering their entry to the human body via inhalation and ingestion pathways. The obtained data may serve as a reference for future 222Rn-related works.
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Radón , Ríos , Humanos , Bangladesh , AguaRESUMEN
Anorectal malformations (ARM) incorporate a broad spectrum of diseases, can affect both sexes, and involve the distal anus and rectum as well as the uro-genital tracts. Defects range from the minor which can be treated easily with an excellent outcome, to those are complex and often associated with other anomalies are difficult to manage with poor functional prognosis. This study was done to observe the hospital incidence of Anorectal malformations, frequency of types, sex distribution and spectrum of associations with ARM. The effects of presence of associated anomalies on morbidity and mortality also observed. Detailed history, clinical examinations and relevant investigations were performed for the primary and as well as the associated anomalies. A total of 80 patients were admitted in the department of pediatric surgery in Mymensingh Medical College Hospital during the period of June 2016 to May 2017. Age of the patients was ranging from 1-180 days with the mean age of 0.49±1.002 months. Male: Female ratio was 1.6: 1. Among them 48(60%) were high and 32(40%) were low variety of ARM. In male 37(46.2%) had high and 13(13.7%) were low variety whereas in female 11(13.7%) were high and 19(23.7%) had low ARM. Associated anomalies were seen in 25(31.2%) patient -18 in males and 7 in females; 20 in high and 5 in low ARM. Associated anomalies were uro-genital 11(13.8%), cardiovascular 10(12.5%), vertebral 4(5%), limb defects 3(3.5%) and others 2(2.5%). Four patients have more than one anomaly. Anorectal malformations occur more in boys than girls. Males were more likely to have high lesions and without fistula was the common defect. Low variety ARM were found more in females with Anovestibular fistula is the commonest defect. The most common associated anomalies were recto urinary fistula (13.8%). Associations were more in high than low ARM but not significant (p>0.05). Post operative complications were more in high ARM in both sexes with associated anomalies. The effects of types and associations on morbidity and mortality were significantly different (p<0.05).
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Malformaciones Anorrectales , Fístula Rectal , Canal Anal , Malformaciones Anorrectales/epidemiología , Niño , Femenino , Humanos , Recién Nacido , Masculino , Recto , Columna VertebralRESUMEN
The structural and magnetic behavior of Mn-site doped intermetallic manganese silicide alloys of nominal compositions Mn5-x A x Si3 (x = 0.05, 0.1, 0.2 and A = Ni, Cr) have been investigated with a focus to the inverted hysteresis behavior and thermomagnetic irreversibility. Room temperature x-ray powder diffraction data confirm that all the doped alloys crystallize in hexagonal D88 type structure with space group P63/mcm. The doped alloys are found to show paramagnetic-collinear antiferromagnetic (AFM2)-noncollinear antiferromagnetic (AFM1) transitions during cooling from room temperature. A significant decrease in the critical values of both AFM1-AFM2 transition temperatures and fields have been observed with the increasing Ni/Cr concentration. Inverted hysteresis loop, field-induced arrest, and thermomagnetic arrest, the key features of the undoped Mn5Si3 alloy, are found to be significantly affected by the Mn-site doping and eventually vanishes with 4% doping.
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The standard treatment of high variety anorectal malformation (ARM) is the staged approach. A growing interest in one stage correction of high variety ARM was noted recently. The aim of this study was to examine the feasibility, safety and outcome of single stage correction of High variety ARM. This prospective study was carried out in the Department of Paediatric Surgery, Mymensingh Medical College Hospital (MMCH), Mymensingh, Bangladesh from July 2012 to September 2013. It was conducted among 30 patients, all having high ARM. The type of ARM was diagnosed by clinical examinations and imaging studies like Invertogram and Transperineal USG. Other associated congenital anomalies were excluded. Out of 30 patients 11(36.67%) were male and 19(63.33%) were female. The age incidence ranges from 2 days to 5 months with the mean±SD age 1.70±1.63 months. The type of fistula present along with ARM in male varies from rectobulber urethral fistula, recto prostatic urethral fistula, recto vesical fistula and in one case without any fistula. Type of fistula was rectovestibular, rectovaginal and one cloacal malformation among the female patients. The treatment modalities in male patients vary in transabdominal pull through anorectoplasty and PSARP, in which the former was done more (72.73%). In case of female patients, treatment modalities vary in transabdominal pull through and anorectoplasty and transfistulous anorectoplasty, with the later done more (52.63%). Regarding post operative complications, there was 1(14.29%) wound infection in male and 1(33.33%) in female, partial wound disruption in 1(14.29%) male patient, mucosal prolapse 2(28.57%) in male and 1(33.33%) in female, anal stenosis 1(14.29%) in male and 1(33.33%) in female, perianal soiling in 1(14.29%) male patient. All the complications were treated by adopting various measures. One stage correction of High variety ARM in both male and female patients is technically feasible. The safeties of this approach depend on the adherence to strict inclusion criteria. The early post operative complications are acceptable and can be managed successfully.
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Malformaciones Anorrectales/cirugía , Fístula Rectal/cirugía , Fístula Rectovaginal/cirugía , Recto/cirugía , Canal Anal/cirugía , Bangladesh , Niño , Procedimientos Quirúrgicos del Sistema Digestivo/efectos adversos , Procedimientos Quirúrgicos del Sistema Digestivo/métodos , Estudios de Factibilidad , Femenino , Humanos , Masculino , Estudios Prospectivos , Fístula Rectal/congénito , Fístula Rectovaginal/congénito , Resultado del TratamientoRESUMEN
The incidence of peptic ulcer has steadily declined through out the world. This decreasing trend is also noticeable in this subcontinent. The point prevalence of peptic ulcer (PUD) in Bangladesh was around 15% in eighties. The aim of this study was to see the present prevalence of peptic ulcer at endoscopy and to identify changing trends in the occurrence of peptic ulcer in Bangladesh. This retrospective analysis of the endoscopic records of multiple tertiary referral centres of Dhaka city were done from January 2012 to July 2013. A total of 5608 subjects were the study samples. We included those patients having peptic ulcer in the form of duodenal ulcer, benign gastric ulcer including pre-pyloric ulcer and gastric outlet obstruction due to peptic ulcer. Duodenal ulcer and benign gastric ulcer were found in 415(7.4%) and 184(3.28%) patients respectively and gastric outlet obstruction due to peptic ulcer was found in 23(0.40%) patients.
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Obstrucción de la Salida Gástrica , Úlcera Péptica , Bangladesh/epidemiología , Obstrucción de la Salida Gástrica/epidemiología , Humanos , Incidencia , Úlcera Péptica/epidemiología , Estudios RetrospectivosRESUMEN
This cross-sectional observational study was conducted in the Department of Gastroenterology, Bangabandhu Sheikh Mujib Medical University, Sir Salimullah Medical College and Mitford Hospital, Holy family Red Crescent Medical College and Hospital and Popular Diagnostic Centre, Shantinagar branch, Dhaka, Bangladesh from January 2012 to July 2013. Study subjects were included in the study irrespective of age and sex having different upper GI tract. Complaints like dysphagia, heart burn, abdominal pain/dyspepsia, nausea, vomiting, haematemesis and/or malena, anorexia, unexplained anemia, weight loss etc. All the findings of oesophagus, stomach and duodenal lesions were observed and data were recorded. The results were considered positive based on the macroscopic appearance using standard diagnostic criteria. During the study period endoscopy was performed in 5608 subjects. Among which 2968(52.92%) were male and 2640(47.07%) were female. The patients age range from 7 years to 108 years. The majority of the patients were from 40-50 years. Normal findings were observed among 3321(59.21%) cases. Gastroduodenal erosions were present among 684(12.19%) cases, Oesophageal varices in 444(7.91%) cases, duodenal ulcer diseases in 415(7.40%) cases and gastric ulcer in 184(3.28%) cases. Ca stomach was found among 165(2.94%) cases. A number of diseases were identified through this procedure. Current study observed a large proportion of patients (40.79%) had positive upper GI endoscopy.
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Endoscopía del Sistema Digestivo , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Bangladesh , Niño , Estudios Transversales , Várices Esofágicas y Gástricas , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Here, we report the development of a simple, small, fast heating, and portable, homemade, inert gas (Ar) atmospheric annealing setup. Instead of using a conventional heating element, a commercial soldering rod having an encapsulated fast heating heater is used here. The sample holder is made of a block of stainless steel. It takes 200 s to reach 700 °C, and 10 min to cool down. The probability of oxidation or surface contamination has been examined by means of x ray photoelectron spectroscopy of virgin Cu sample after annealing at 600 °C. In addition, we compare the annealing of a hydrogenated carbon nitride film (HCN(x)) in both a conventional vacuum and our newly developed ambient Ar atmosphere setup.
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INTRODUCTION: Pneumatosis intestinalis is a rare condition affecting 0.03% of the population. It has a myriad of aetiological causes and hence presentation can vary immensely. The management of symptomatic pneumatosis intestinalis in an acute and outpatient setting remains a challenge to both physicians and surgeons. CASE PRESENTATION: We present a case of a 79 year old who presented in a gastroenterology outpatients department with a history suggestive of intermittent small bowel obstruction associated with abdominal pain aggravated by eating and posture. He was found to have signs suggestive of Marfan's syndrome. Computed tomography demonstrated extensive pneumatosis intestinalis of the small bowel. Due to deterioration in symptoms, an exploratory laparotomy was performed demonstrating segmental small bowel pneumatosis intestinalis secondary to a hypermobile mesentery. CONCLUSION: This case highlights the importance of both surgical and gastroenterology expertise in successfully managing symptomatic pneumatosis intestinalis.
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The aim of the present study was to examine whether chicken sperm express mRNA of Toll-like receptors (TLR) and avian ß-defensins (avBD), and whether the expression of avBD in the sperm are regulated by the interaction of TLR-2 and TLR-4 with their corresponding ligands. Total RNA was extracted from fresh sperm and mRNA expression for TLR and avBD were examined by reverse-transcription PCR. Changes in the expression of avBD-5, avBD-9, avBD-10, and avBD-12 in the sperm after incubation with lipopolysaccharide (LPS; TLR-4 ligand) or lipopeptide (Pam3CSK4; TLR-2 ligand) were examined by real-time PCR. Among 8 types of TLR, PCR products of 7 types, namely, TLR-2 to TLR-5, TLR-7, TLR-15, and TLR-21, were identified in the sperm. The sperm also expressed cluster of differentiation 14 (CD14), which is required for the binding of LPS to TLR-4. Out of 14 avBD types reported in the chicken, a total of 9 types, namely, avBD-1, avBD-3, avBD-5, and avBD-7 to avBD-12 were expressed by the sperm, whereas avBD-5, avBD-9, avBD-10, and avBD-12 showed dense signals on the gel. Expression levels of avBD-5, avBD-9, avBD-10, and avBD-12 were significantly increased in the sperm stimulated with LPS, and that of avBD-5 was also enhanced by Pam3CSK4. These results suggest that avian sperm have the ability of the innate host defense to protect themselves from infection in the male and female genital tracts.
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Pollos , Espermatozoides/efectos de los fármacos , Espermatozoides/metabolismo , Receptores Toll-Like/metabolismo , beta-Defensinas/metabolismo , Animales , Bacterias , Regulación de la Expresión Génica/fisiología , Inmunidad Innata , Ligandos , Lipopolisacáridos/farmacología , Masculino , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptores Toll-Like/genética , beta-Defensinas/genéticaRESUMEN
Thirty-one Tocklai vegetative (TV) tea clones contained caffeine and total catechin 44.39 and 227.55 mg/g dry weight of leaves, respectively. The (-)-epigallocatechin gallate (EGCG) was the most abundant (109.60 mg/g) followed by -(-)-epigallocatechin (EGC, 44.54 mg/g), (-)-epicatechin gallate (ECG, 41.74 mg/g), (-)-epicatechin (EC, 27.42 mg/g) and +catechin (4.25 mg/g). Total catechins were highest in TV 20 (509.7 mg/g) and lowest in TV 6 (71.7 mg/g). The tea clones that contain high level of total catechin exhibited the strongest antimicrobial activity. Among caffeine and flavanol compounds, theaflavins (TF) present in black tea possess a similar antimicrobial potency as EC present in fresh leaves, and that the conversion of catechins to TF during fermentation in making black tea tends to alter their antimicrobial activities. The bioactive molecules other than catechins present in tea leaves may also contribute towards antimicrobial activity.
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India has a large repository of important tea accessions and, therefore, plays a major role in improving production and quality of tea across the world. Using seven AFLP primer combinations, we analyzed 123 commercially important tea accessions representing major populations in India. The overall genetic similarity recorded was 51%. No significant differences were recorded in average genetic similarity among tea populations cultivated in various geographic regions (northwest 0.60, northeast and south both 0.59). UPGMA cluster analysis grouped the tea accessions according to geographic locations, with a bias toward China or Assam/Cambod types. Cluster analysis results were congruent with principal component analysis. Further, analysis of molecular variance detected a high level of genetic variation (85%) within and limited genetic variation (15%) among the populations, suggesting their origin from a similar genetic pool.
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Análisis del Polimorfismo de Longitud de Fragmentos Amplificados/métodos , Variación Genética , Semillas/genética , Té/economía , Té/genética , Cartilla de ADN/metabolismo , India , Filogenia , Análisis de Componente PrincipalRESUMEN
BACKGROUND & OBJECTIVES: There is scarcity of information on impact of iodine deficiency on growth and development of newborns, both pre-term and term babies. The present study was carried out to see the impact of iodine deficiency (ID) on the intrauterine foetal growth and development in terms of birth weight (BW) in an African population living in an iodine deficient zone. METHODS: The maternal and cord serum thyroid parameters at term delivery (38-40 wk of gestation) were measured and correlated with the birth weights of the corresponding newborns, and the results compared between those assessed in an ID zone (Jengere region of Bassa district, Plateau State, Nigeria) and with that of non ID (Control) zone, Jos, the State capital. RESULTS: The level of maternal and neonatal thyroid function were significantly reduced in the group with low birth weights (<2.5 kg) as compared to the level seen in normal birth weights category in both control (Jos) and ID (Jengere) regions. Both in non ID and ID regions the maternal serum thyroxine (T4), thyroxine binding globulin (TBG) and free thyroxine index (FT4I) were positively correlated (P<0.005) with BW of term babies while thyroid stimulating hormone (TSH) (P<0.005) and thyroxine binding capacity (TBK) (P<0.05) showed negative correlation. Maternal T3 and T3/T4 ratio did not show any correlation with BW in Jos, while in the ID region of Bassa the BW showed a positive correlation (P<0.005) with the maternal serum T3 and T3/T4 ratio. Cord serum analysis of the term babies revealed that the BW was positively correlated with its T4, triiodothyronine (T3), TBG, FT4I and T4/TBG ratio (Jos only) (P<0.005) in both ID region of Bassa and non ID control zone of Jos. Cord serum TSH and TBK showed negative correlation (P<0.005) with BW. Thyrotrophin releasing hormone (TRH) stimulation test revealed that mothers with small for date (SFD) babies at term were more hypothyroid compared to the level of thyroid function seen in the women delivering normal babies. INTERPRETATION & CONCLUSION: Maternal thyroid hormone plays a significant role in the intrauterine foetal growth and development, and the smaller babies and their corresponding mothers, in an ID affected area are functionally sub-thyroid in greater proportion due to prevailing long standing environmental iodine deficiency (EID). Maternal T3 is an important factor in the defence of foetus in ID regions.
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Desarrollo Fetal , Yodo/deficiencia , Adolescente , Adulto , Peso al Nacer , Femenino , Humanos , Recién Nacido , Nigeria , Embarazo , Hormonas Tiroideas/sangre , Tirotropina/sangreRESUMEN
Falciparum malaria is an ongoing problem in the foothills of Northeast India. Evaluation of the drug sensitivities of P. falciparum was carried out in four endemic villages of the Sonitpur District of Assam, involving 218 cases who were tested in vivo over 35 days. Chloroquine resistance was detected at the RI level in 29 cases (13%) and RII level in 8 cases (4%). No RIII chloroquine resistant cases were detected in the study. RI resistance was observed in the age groups 6-10 years, 11-14 years, and 15 years and above in 16%, 17%, and 13%, respectively. RII level resistance was observed in 4% of all those groups combined. All the RI and RII resistant cases responded well to a single dosage of Metakelfin (sulfamethoxypyrazine I.P 1,500 mg and pyrimethamine I.P 75 mg).
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Antimaláricos/farmacología , Cloroquina/farmacología , Resistencia a Medicamentos , Malaria Falciparum/tratamiento farmacológico , Plasmodium falciparum/efectos de los fármacos , Adolescente , Adulto , Factores de Edad , Animales , Antimaláricos/uso terapéutico , Niño , Preescolar , Cloroquina/uso terapéutico , Combinación de Medicamentos , Enfermedades Endémicas , Humanos , India/epidemiología , Lactante , Malaria Falciparum/parasitología , Persona de Mediana Edad , Pirimetamina/uso terapéutico , Población Rural , Sulfaleno/uso terapéuticoRESUMEN
An investigation was conducted to determine the distribution, virulence gene profile and phenotypes of Shiga toxin-producing Escherichia coli (STEC) strains within a dairy farm in Kolkata, India by characterizing the STEC strains isolated from healthy dairy cow and calf stool samples, raw milk and farm floor swabs from July 2001 to March 2002. Primary screening by multiplex-PCR detected stx1 and stx2, the common virulence genes of STEC, in 18.9% of cow faeces, 32.4% of calf stool samples, 21.6% of farm floor swabs and 4.5% of raw milk samples and viable STEC were recovered from 4.5, 9.9, 8.1 and 1.8% of the corresponding PCR-positive samples. Strains harbouring stx1 (63.3%) and hlyA (53.3%) were frequently detected compared to eae (13.3%). Most of the strains harboured similar sets of reported virulence genes common among isolates from diarrhoea patients. Most of the strains also exhibited multidrug resistance, sorbitol fermentation and produced enterohaemolysin. The randomly amplified polymorphic DNA-PCR (RAPD-PCR) profile of the STEC strains isolated from the farm milieu revealed diverse banding patterns and clonal analysis demonstrated that the strains from different sources were not identical but showed some genetic relatedness. The study demonstrates the potential of dairy farm for housing virulent STEC.
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Proteínas Bacterianas/genética , Infecciones por Escherichia coli/epidemiología , Escherichia coli/patogenicidad , Técnica del ADN Polimorfo Amplificado Aleatorio , Toxinas Shiga/biosíntesis , Animales , Antibacterianos/farmacología , Secuencia de Bases , Bovinos , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/microbiología , ADN Bacteriano/análisis , Industria Lechera , Farmacorresistencia Bacteriana , Escherichia coli/efectos de los fármacos , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/diagnóstico , Heces/microbiología , Humanos , India/epidemiología , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Población Rural , Índice de Severidad de la Enfermedad , Especificidad de la EspecieRESUMEN
To investigate the level of neonatal chemical hypothyroidism (NCH), thyroid function was measured in the cord blood of 271 neonates in an area with a high prevalence of endemic goitre, Bassa (n = 140), and a nonendemic area, Jos (n = 131), in Plateau State, Nigeria. NCH was defined as T4 < 40 nmol/L and TSH > 49 mU/L and borderline compensated NCH as T4 31-50 nmol/L and TSH 41-49 mU/L. NCH was detected in 16.4% of neonates in Bassa and in 3.8% in Jos. Borderline compensated NCH was detected in an additional 18.6% in Bassa and 8.4% in Jos. Contamination of foodstuffs and water by iodine-containing antiseptics and disinfectants during the antenatal period, in addition to factors responsible for endemic goitre, e.g. iodine deficiency and goitrogens, are considered to be responsible for the high prevalence of NCH in the Bassa area. As most infants' thyroid function recovers with time, the former might be a more important factor in the aetiology of NCH.
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Hipotiroidismo/epidemiología , Hormonas Tiroideas/sangre , Hipotiroidismo Congénito , Sangre Fetal/metabolismo , Humanos , Hipotiroidismo/sangre , Recién Nacido , Nigeria/epidemiología , Tirotropina/sangre , Tiroxina/sangre , Triyodotironina/sangreRESUMEN
BACKGROUND & OBJECTIVES: Detailed epidemiological and entomological studies were undertaken in forest-fringed villages and a Tea Estate in Sonitpur, Assam to assess the malaria situation. METHODS: Door-to-door active surveillance was carried out to collect blood samples. Thick and thin blood smears stained with Giemsa were used for malaria parasite detection. Mosquito collections were made using CDC miniature light-traps and hand catch methods from dusk-to-dawn. RESULTS: 48% SPR, 49.1 Pf% was recorded from the study villages. Children between 10 and 14 years were most sufferers. Per trap night density of mosquitoes in human dwellings was 204.3 and in cattlesheds--908.7, An. minimus accounted for 20.7% of total malaria vectors. INTERPRETATION & CONCLUSION: The results showed high malaria risk in the study villages. High vector density with high parity rate, poor socio-economic conditions, lack of awareness, poor sanitation and congenial atmosphere for mosquito proliferation are aggravating the malaria situation more complex in the study area.
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Anopheles , Malaria/epidemiología , Parasitemia/epidemiología , Adolescente , Adulto , Agricultura , Animales , Colorantes Azulados , Niño , Preescolar , Humanos , India/epidemiología , Lactante , Recién Nacido , ÁrbolesRESUMEN
The present study was undertaken to evaluate the effect of growth medium on expression of hydrophobicity of Staphylococcus epidermidis. A total of 24 hydrophobic isolates of S. epidermidis, determined by n-hexadecane adherence assay (HAA) earlier were included. Five different growth media: horse blood agar (HBA), brain heart infusion agar (BHIA), brain heart infusion broth (BHIB), tryptic soy broth (TSB) and proteose peptone broth (PPB) were used. All 24 isolates exhibited the reproducible hydrophobicity when grown on HBA; however, 20 (83.33%), 19 (79.16%), 15 (62.50%) and 13 (54.16%) isolates were found to be hydrophobic when grown in BHIA, BHIB, TSB and PPB, respectively. HBA was found to be the most suitable medium for detection of hydrophobicity of S. epidermidis followed by BHIA or BHIB.
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Medios de Cultivo/farmacología , Interacciones Hidrofóbicas e Hidrofílicas , Staphylococcus epidermidis/efectos de los fármacos , Staphylococcus epidermidis/fisiología , Animales , Caballos , Reproducibilidad de los ResultadosRESUMEN
This study was conducted to investigate the diagnostic performance characteristics of prostate specific antigen (PSA) by comparing serum PSA value with histological findings in patients suspevted of having prostate cancer in Aminu Kano Teaching Hospital. Nigeria. Clinical and Laboratory records were examined and collated for serum PSA values, together with histological findings of biopsy specimen, clinical diagnosis, age of patients, and mode of presentation. The serum PSA values were determined by ELECSYS 1010 autoanalysers Roche, Germany based on electrochemiluminescence immunoassay technique. The results show that serum PSA values increase with age in the assymptomatic non-cancer patients who came for medical check up but were within normal limit. In prostatic disease conditions PSA values were raised in benign prostatic hyperplasia 35.957± 4.0315ng/ml, in undifferentiated carcinoma 56.22±4.295ng/ml and adenocarcinoma >100ng/ml as compared to the normal range (0-4ng/ml). These cases were confirmed by histological diagnosis. It is concluded that PSA evaluations is a sensitive marker for prostate cancer but because of various other conditions that affect serum PSA concentration, other methods of investigations such as Digital Rectal examination, Trans Urethral Ultra-Sonography and histological examination should be combined to confirm diagnosis. Prognosis of patients will be better if early diagnosis is made.