Suicide and the war in Croatia.

Analyzing data from the Institute of Forensic Medicine (IFM) in Rijeka, a total of 853 suicides were recorded in a 15 years period (1986-2000). Quantitative and qualitative features of suicides were analyzed in three intervals: pre-wartime, wartime and post-wartime. In the wartime period (1991-1995), the suicide rate increased by 20.9% in comparison with the pre-war period. In the post-wartime period, the suicide rate dropped by 26.2% in comparison with the war period. The results show a significant increase in suicide rates in the wartime. During the wartime period, a large number of suicide victims under the age of forty increased-45.3% in comparison with the pre-war period and 56.6% in comparison with the post-war period. The use of firearms as a means of committing suicide quadrupled during the war in comparison with the pre-war period. The level of alcohol intoxication of perpetrators at the moment of suicide significantly increased in the wartime period. War had a direct impact on quantitative and qualitative characteristics of suicides in Croatia.

DNA typing from skeletal remains: evaluation of multiplex and megaplex STR systems on DNA isolated from bone and teeth samples.

AIM: To evaluate the performance of three multiplex short tandem repeat (STR) systems (AmpflSTR Profiler, AmpflSTR Profiler Plus, and AmpflSTR COfiler), and a megaplex STR system (PowerPlex 16) on DNA extracted from the skeletal remains. By performing a microbial DNA challenge study, we also evaluated the influence of microbial DNA on human DNA typing. METHODS: A subset of 86 DNA extracts isolated from 8-50 years old bone and teeth samples, corresponding to 20 identification cases from mass graves in Croatia and Bosnia and Herzegovina, and to 4 paternity cases involving deceased parents in Spain, were analyzed by the above systems. RESULTS: Bone samples with no detectable human DNA (tested with Quantiblot), as well as teeth samples with detectable human DNA, were successfully amplified. Surprisingly, even in highly degraded samples, PowerPlex 16 offered very robust amplification for the both Penta E and Penta D markers. We observed a few non-specific extra peaks of 202 and 308 base pairs, which appeared to match 16S rRNA of the Pseudomonas halodenitrificans. CONCLUSION: AmpflSTR Profiler Kit, AmpflSTR Profiler Plus Kit, the AmpflSTR COfiler Kit, and the PowerPlex 16 system are very sensitive multiplex STR amplification systems, which can be successfully used to obtain a multilocus STR profile from old teeth and bone samples with minimal amounts (pg) of human DNA or even with no detectable human DNA.

Diving-related fatalities caused by underwater explosions: a report of two cases.

The authors report two cases of diving-related water blast with fatal outcome resulting from nearby underwater explosions. Water blast with fatal outcome almost exclusively occurs in wars at sea. Underwater explosions are extremely rare in diving because of the limited exposure. Forensic findings in both cases reported included expected injuries to gas-filled organs such as the middle ear, lungs, and intestine; some rarely described injuries such as rupture of the liver, spleen, and kidneys; and also some lesions that were not found in a search of the literature: rupture of the heart and contusion of the hypophysis. Injuries caused by fatal underwater explosions should be carefully evaluated in forensic medicine to provide data that may support a criminal investigation.

Thymus alterations related to intravenous drug abuse.

It has been noticed on forensic material that Hassall's corpuscles of the thymus are more often calcified in intravenous drug abusers than in healthy persons. Thymuses of 15 intravenous heroin addicts were histologically examined and compared with thymuses of 15 healthy persons. Acute bleeding and dystrophic calcification in the thymocyte's parenchyma were more common among intravenous drug abusers (p = .005 and p = .001, respectively). The degree of physiologic involution measured by fatty replacement and the clarity of border between cortex and medulla was equal. No significant diversity was found in the features of Hassall's bodies. Our study emphasizes the necessity for a defined criteria of morphologic changes in the thymus that could be expected in intravenous drug abusers. In that way it would be possible to complete the forensic findings as well as to examine immunologic system alterations of that risk population.

Homosexual homicides.

This report describes four cases of male homosexual homicide committed in Split County, Croatia, in 1991 and 1995. The homosexual orientation of the victims was determined by police investigation. Three middle-aged to elderly person were killed by an unknown offender or offenders, in very similar ways within a few months of end other in 1991. The bodies were found in their houses with multiple blunt force injuries to the head. One victim also had marks of manual strangulation, and was choked with a handkerchief which had been put in his mouth. The last victim was a 45-year-old man, found in the bathroom with 33 stab wounds, mostly in the neck and back, and with two shots in the head from an underwater gun. He was killed by two adolescent males at the beginning of sexual intercourse. The presented cases show a high level of violence with multiple injuries may be associated with male homosexual homicide.

Deaths of drug addicts in Split.

Since the beginning of the war in Croatia, the number of drug addicts and of fatal intoxications in the Split region has increased. Drug-related fatalities (n = 25) were investigated over a 12-month period in 1995. Data about the cause of death were based on the information from police reports, autopsy findings and toxicological analyses. The main drug involved was heroin. Other drugs were rarely reported. Blood tests for alcohol were performed in all cases. Alcohol consumption increased the risk of heroin overdosing.

Identification of war victims from mass graves in Croatia, Bosnia, and Herzegovina by use of standard forensic methods and DNA typing.

The postmortem remains of sixty-one war victims were excavated from 6 mass graves in Bosnia and Herzegovina one and a half years after interment Using standard identification methods, including the matching of medical and dental records, the recognition of distinguishing characteristics such as the use of clothing and belongings, and video superimposition, 35 persons were identified. For the remaining 26 persons identification efforts continue. DNA typing was performed at the HLA DQA1 locus and five PM system loci. Results from DNA typing were confirmed by other methods. DNA profiles of family members of 150 missing persons are now being developed using the 6 loci. These DNA profiles will then be compared with those generated from the bone and teeth remains of the unidentified victims.

Northern and southern Croatian population data on seven PCR-based loci.

Northern and southern Croatian sample populations were typed at seven PCR-based loci -LDLR, GYPA, HBGG, D7S8, Gc, HLA-DQA1 and D1S80. The results show that all loci meet Hardy-Weinberg expectations and that there is little evidence for association of alleles between loci. Allelic frequency distributions at all loci, except HLA-DQA1, show no differences between the northern and southern Croatian sample populations. Moreover, the population data for Croatians are similar to U.S. Caucasians; only HLA-DQA1 for southern Croatians was statistically different compared with U.S. Caucasians. A Croatian population database(s) has been created and can be used for forensic analyses to estimate the frequency of a multiple locus DNA profile.

Fatal firearm injuries caused by handmade weapons.

Six cases of accidental death caused by a shot from handmade or improvised firearms are described. Data were collected on incident, victim and special autopsy findings. In three cases the weapon used was a so-called 'pen gun' of small calibre (5.6 mm) and in two cases a home-made shotgun/muzzle loader with shotgun cartridge (containing 16 mm pellets) was used. In one case, the weapon was a shop-made gun with home-made ammunition. It is perhaps surprising that in the present war situation in Croatia, when all kinds of firearms are easily available, people continue to produce handmade weapons.