RESUMEN
The experiments presented herein were designed to probe a potential role for the activation of the LH/CG receptor (LHR) on the receptor-mediated endocytosis of human CG (hCG). Two mutants of the rat LHR (rLHR) that bind the hormone with high affinity but are deficient in signal transduction were prepared by mutating highly conserved residues that have been previously shown to be important in signal transduction in other members of the G protein-coupled receptor family. Mutation of a highly conserved aspartic acid in the second transmembrane domain of the rLHR (designated rLHR-D383N) does not affect hCG binding but impairs signal transduction. When compared with cells expressing an equivalent density of wild type rLHR (rLHR-wt), concentration-response curves for the hCG-stimulated cAMP accumulation in cells expressing rLRH-D383N- are characterized by an 18-fold increase in the EC50 but no change in the maximal response. Cells expressing rLHR-D383N also display a 4- to 5-fold increase in the half-life of internalization of hCG. Mutation of a highly conserved arginine in the second intracellular loop of the rLHR (designated rLHR-R442H) also does not affect hCG binding but impairs signal transduction. When compared with cells expressing an equivalent density of rLHR-wt, concentration-response curves for the hCG-stimulated cAMP accumulation in cells expressing rLHR-R442H are characterized by a 7-fold increase in the EC50 and a 6- to 10-fold decrease in the maximal response. Cells expressing rLHR-R442H also display a 1.5- to 2-fold increase in the half-life of internalization of hCG. These results, together with the finding that an antagonist of hCG is internalized more slowly than hCG, suggest that the activation of the LHR is needed for the efficient endocytosis of the bound hCG.
Asunto(s)
Endocitosis , Mutagénesis , Receptores de HL/genética , Receptores de HL/fisiología , Transducción de Señal , Arginina , Asparagina , Ácido Aspártico , Línea Celular , Gonadotropina Coriónica/metabolismo , Gonadotropina Coriónica/farmacología , AMP Cíclico/metabolismo , Embrión de Mamíferos , Histidina , Humanos , Riñón , Conformación Proteica , Receptores de HL/química , TransfecciónRESUMEN
Exposure to solar ultraviolet (UV) light is a major cause of skin cancer, the most common human neoplasm. The earth's upper atmosphere absorbs the high energy UV-C wavelengths (100-280 nm), while allowing transmission of UV-B (280-320 nm) and UV-A (320-400 nm). It is therefore UV-B and to some extent UV-A, that contributes to most human skin malignancies. We report that the exposure of cultured keratinocytes or skin to UV-C radiation causes activation of MAP kinases (ERK and JNK). In contrast, the solar radiation associated with skin cancer (UV-B) was an ineffective activator of the ERK and JNK signal transduction pathways. Therefore, while exposure of epidermal cells to UV-C radiation under laboratory conditions causes marked activation of MAP kinase signal transduction pathways, only a low level of MAP kinase signaling is involved in the response of skin to biologically relevant solar radiation.
Asunto(s)
Proteínas Quinasas Dependientes de Calcio-Calmodulina/efectos de la radiación , Queratinocitos/efectos de la radiación , Proteínas Quinasas Activadas por Mitógenos , Transducción de Señal/efectos de la radiación , Rayos Ultravioleta/efectos adversos , Animales , Células CHO/enzimología , Células CHO/efectos de la radiación , Proteínas Quinasas Dependientes de Calcio-Calmodulina/biosíntesis , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Células Cultivadas , Cricetinae , Activación Enzimática/efectos de la radiación , Inducción Enzimática/efectos de la radiación , Femenino , Regulación de la Expresión Génica , Genes ras , Proteínas Quinasas JNK Activadas por Mitógenos , Queratinocitos/enzimología , Ratones , Ratones Pelados , Proteína Quinasa 1 Activada por Mitógenos , Proteína Quinasa 3 Activada por Mitógenos , Proteínas Tirosina Quinasas/metabolismo , Proteínas Tirosina Quinasas/efectos de la radiación , Piel/enzimología , Piel/efectos de la radiación , Proteínas ras/biosíntesis , Proteínas ras/genéticaRESUMEN
Exposure of mammalian cells to UV radiation and other DNA-damaging agents triggers a response known as the UV response. This induction response involves a large number of genes including c-jun, cell-cycle regulatory proteins, specific repair enzymes, and the tumor suppressor gene p53. Altered expression of these genes following DNA damage is hypothesized to result in G1 arrest, thereby allowing cells to repair DNA damage prior to cell division. In the present study, we investigated expression of the p53 gene in mouse keratinocyte cell line 308 after exposure to UV-B light at a biologically relevant dose. Irradiation of 308 cells with 40 J/m2 UV-B resulted in a 4- to 10-fold induction in the level of p53 protein, peaking at 5 h post-irradiation. Northern blot analysis of RNA from UV-B irradiated cells showed no change in the steady-state level of p53 mRNA following irradiation. However, the half-life of p53 protein in UV-B irradiated 308 cells was extended approximately 7-fold, from 30 to 200 min. Additional studies were performed with specific anti-p53 monoclonal antibodies to establish whether UV-B irradiation induced a conformational change in p53 protein in irradiated cells. Metabolic labeling with 35S-methionine followed by immunoprecipitation with p53 monoclonal antibody PAb246, which recognizes the wild-type murine p53 protein, demonstrated that the p53 protein present in 308 cells possessed the wild-type conformation both before and after UV-B irradiation. In contrast, p53 antibody PAb240, which recognizes a 'conformation-dependent' epitope, was not reactive with the p53 protein present in 308 cells. Therefore, we conclude that the induction of p53 protein in mouse keratinocytes following UV-B irradiation occurred posttranscriptionally, and was due to a significant increase in p53 protein half-life.
Asunto(s)
Queratinocitos/efectos de la radiación , Proteína p53 Supresora de Tumor/metabolismo , Animales , Línea Celular , Semivida , Queratinocitos/metabolismo , Ratones , Ratones Endogámicos BALB C , Conformación Proteica , ARN Mensajero/análisis , Proteína p53 Supresora de Tumor/química , Proteína p53 Supresora de Tumor/genética , Rayos UltravioletaRESUMEN
Recent reports implicate two DNA tumour viruses, herpes simplex virus type 2 (HSV-2) and human papillomavirus types 16 or 18 (HPV-16 and -18), in the pathogenesis of cervical cancer. Previous studies have indicated that primary human fibroblasts transfected with HPV-16 and HSV-2 morphological transforming region III (mtr III) are more aneuploid than fibroblasts immortalized with HPV-16 and that HSV-2 DNA sequences are retained in transformed cells. Since HSV-2 and HPV typically infect cells of epithelial origin, the interactions of these viruses with respect to morphological transformation were examined in human keratinocytes. HPV-16- or HPV-18-immortalized keratinocytes (FEPL and FEA cells, respectively) were transfected with fragments derived from HSV-2 mtr III. When compared to their normal counterparts, FEPL cells and FEA cells transfected with mtr III fragments grew to higher saturation densities and were morphologically transformed. FEPL cells transformed by HSV-2 were capable of growth in soft agar and, when injected into nu/nu mice, lesions developed at the site of injection. Histological examination of the lesions revealed a benign mass which was composed of squamous epithelial cells that were producing keratin. In contrast, immortalized keratinocytes (FEPL or FEA) or FEA cells transfected with HSV-2 did not produce these lesions. These observations suggest that sequences within mtr III can alter the growth properties of human keratinocytes immortalized by HPV-16 or HPV-18.
Asunto(s)
Transformación Celular Neoplásica/genética , Transformación Celular Viral/genética , Queratinocitos/microbiología , Papillomaviridae/genética , Simplexvirus/genética , Animales , Secuencia de Bases , Línea Celular Transformada , ADN Viral/genética , Genes myc/genética , Genes p53/genética , Genoma Viral , Humanos , Ratones , Ratones Desnudos , Datos de Secuencia Molecular , Neoplasias Experimentales/microbiología , ARN Viral/análisisRESUMEN
Expression of herpes simplex virus 2 (HSV-2)-encoded ribonucleotide reductase (RR) is required for growth in nondividing cells. The functional enzyme is composed of a large and a small subunit. In virus-infected cells, RR is expressed temporally as a delayed early protein. However, the promoter regulatory region of the large subunit can function as an immediate early promoter in transient transfection assays, suggesting that expression may be quite complex. In this study, a 95-bp fragment derived from the open reading frame of the large subunit of RR (RR-A) functioned as a silencer when placed adjacent to a heterologous promoter. If the fragment was placed distal to the promoter, repression was relieved and in human keratinocytes promoter activity was consistently higher than control constructs. Exonuclease III protection assays revealed that nuclear factors from human keratinocytes as well as other primate cells specifically bind to this fragment. A 30-bp motif containing a consensus SP-1 binding site and an alternating Pu/Py element was protected in all cell lines. These results suggest that a 95-bp fragment in the open reading frame of HSV-2 RR-A plays a role in regulating viral gene expression.
Asunto(s)
Secuencias Reguladoras de Ácidos Nucleicos/fisiología , Ribonucleótido Reductasas/genética , Simplexvirus/genética , Animales , Secuencia de Bases , Unión Competitiva , Línea Celular , Núcleo Celular , Cloranfenicol O-Acetiltransferasa/genética , Chlorocebus aethiops , Proteínas de Unión al ADN , Exodesoxirribonucleasas , Fibroblastos , Regulación Viral de la Expresión Génica/genética , Humanos , Queratinocitos , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Sistemas de Lectura Abierta/genética , Regiones Promotoras Genéticas , Proteínas Recombinantes de Fusión , Proteínas Represoras/genética , Células Tumorales CultivadasRESUMEN
Human papilloma virus type 16 (HPV-16) and herpes simplex virus type 2 (HSV-2) are human viruses implicated in the development of cancer, in particular cervical cancer. The ability of HSV-2 and HPV-16 to transform early passage human cells was examined in this report. For these studies, gingival fibroblasts were utilized. One gingival cell strain was derived from a normal individual (N-16). The second cell strain was derived from hyperplastic gingival tissue of an epileptic individual (R-30) treated with phenytoin, an antiseizure drug. A common side effect of phenytoin is the induction of gingival overgrowth. R-30 cells contained a stable chromosomal translocation between chromosomes 8 and 18 and expressed higher steady state levels of c-myc. HPV-16 DNA efficiently immortalized R-30 cells but not N-16 cells. R-30 cells cotransfected with HPV-16, and HSV-2 viral DNAs were more aneuploid than R-30 cells transfected with HPV-16 DNA alone. Additionally, R-30 cells cotransfected with both viral DNAs grew better in soft agar than R-30 cells transfected with HPV-16 DNA alone. HSV-2 DNA was detected in transformed cells by polymerase chain reaction. These results suggested R-30 cells were immortalized more efficiently by HPV-16 and further imply that HPV-16 and HSV-2 DNA fragments can cooperate during multistep transformation.