RESUMEN
Serum samples (n = 457) from wolves (Canis lupus) in northern Minnesota were collected from 1972 through 1986 and were tested for antibodies against Leptospira interrogans using a microtiter agglutination test. Twelve serovars included in the study were: australis, autumnalis, ballum, bataviae, bratislava, canicola, copenhageni, grippotyphosa, hardjo, pomona, pyrogenes, and tarassovi. Fifty-two (11%) sera had antibody titers of greater than or equal to 1:50 against one or more serovars of L. interrogans. The seroprevalence of different serovars in decreasing order was: grippotyphosa, bratislava, autumnalis, canicola, pomona, ballum, pyrogenes, hardjo, and copenhageni. No antibodies were found against australis, bataviae, and tarassovi. These results indicate that L. interrogans infection may occur in wolves of Minnesota.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Carnívoros , Leptospira interrogans/inmunología , Enfermedad de Weil/veterinaria , Pruebas de Aglutinación , Animales , Femenino , Leptospira interrogans/clasificación , Masculino , Minnesota/epidemiología , Prevalencia , Factores Sexuales , Enfermedad de Weil/epidemiologíaRESUMEN
In 222 Rana pipiens frogs and 34 tadpoles captured in and near Minnesota, Aeromonas hydrophila and 29 species of Enterobacteriaceae, including yersinia enterocolitica and Salmonella arizonae, were isolated from intestines. The prevalence of members of the family Enterobacteriaceae was lowest in frogs captured in early spring and highest in frogs captured in late summer.
Asunto(s)
Aeromonas/aislamiento & purificación , Enterobacteriaceae/aislamiento & purificación , Rana pipiens/microbiología , Animales , MinnesotaAsunto(s)
Enfermedades de los Gatos/transmisión , Rabia/transmisión , Rabia/veterinaria , Adolescente , Adulto , Animales , Mordeduras y Picaduras/epidemiología , Gatos , Niño , Preescolar , Femenino , Humanos , Lactante , Recién Nacido , Iowa , Masculino , Minnesota , Rabia/prevención & control , Vacunas Antirrábicas/administración & dosificaciónRESUMEN
Frogs and tadpoles were captured at 14 sites in and near Minnesota during 1978-79 and nearly all appeared healthy. Aeromonas hydrophila was isolated from 94 of 294 (32%) juvenile and adult frogs and from 66 of 104 (63%) tadpoles. Of the isolates from frogs and tadpoles respectively, 68% and 47% were from the intestine only, 12% and 32% were from the intestine and the other sites, and 20% and 21% were from extraintestinal sites only. Isolations were more frequent from frogs collected in March-June than in August-November. Evidence was not found that disease due to Aeromonas hydrophila was a primary cause of declining Rana pipiens populations in Minnesota.
Asunto(s)
Aeromonas/aislamiento & purificación , Rana pipiens/microbiología , Animales , Femenino , Intestinos/microbiología , Larva/microbiología , Masculino , Minnesota , Músculos/microbiología , Estaciones del Año , Microbiología del AguaAsunto(s)
Agricultura , Heces/microbiología , Salmonella typhimurium/aislamiento & purificación , Animales , Técnicas Bacteriológicas , Supervivencia Celular , Medios de Cultivo , Técnica del Anticuerpo Fluorescente , Concentración de Iones de Hidrógeno , Modelos Biológicos , Oxidación-Reducción , Estaciones del Año , Aguas del Alcantarillado , Temperatura , Factores de TiempoAsunto(s)
Leptospira/aislamiento & purificación , Leptospirosis/epidemiología , Microbiología del Agua , Adolescente , Adulto , Animales , Sangre/microbiología , Niño , Femenino , Cobayas , Dureza , Humanos , Concentración de Iones de Hidrógeno , Riñón/microbiología , Leptospira/crecimiento & desarrollo , Leptospira interrogans/aislamiento & purificación , Leptospirosis/etiología , Masculino , Persona de Mediana Edad , Factores de Tiempo , Estados Unidos , Virulencia , ZoonosisAsunto(s)
Bovinos , Heces/microbiología , Leptospira/aislamiento & purificación , Animales , Enfermedades de los Bovinos/microbiología , Cricetinae , Técnica del Anticuerpo Fluorescente , Humanos , Concentración de Iones de Hidrógeno , Leptospira/crecimiento & desarrollo , Leptospira/inmunología , Leptospirosis/microbiología , Leptospirosis/veterinaria , Salud Pública , Estaciones del Año , Temperatura , Factores de Tiempo , ZoonosisRESUMEN
To gain additional information on the extent of leptospirosis in wildlife following a human outbreak in Iowa, wild mammals and lower forms of life were collected. Isolation, darkfield microscopic, serologic and pathologic procedures were used to identify past or present evidence of leptospiral infection. Leptospires were isolated from 7 of 75 (9%) mammals. Serotype grippotyphosa was isolated from three raccoons (procyon lotor) and one Western Harvest Mouse (Reithrodontomys megalotis). Serotype ballum was isolated from three opossums (Didelphis marsupialis). Leptospires, unidentified to date, were isolated from frog (Rana pipiens) kidneys. Other positive serologic and pathologic tests gave evidence of infection or previous infection. Utilization of Darkfield microscopic and silver staining techniques did not detect all cases of leptospiral infection. Macroscopic and microscopic serologic methods failed to identify evidence of leptospirosis in all mammals from which leptospires were isolated. Pathologic lesions could only be considered presumptive evidence for leptospirosis. These findings indicate that detection of leptospirosis in wildlife cannot be limited to a single diagnostic test. A combination of diagnostic procedures and clinical evaluation is necessary. Although serotype pomona was implicated as the predominant infecting leptospire in the human cases and domestic animals and was isolated from water at a swimming site, only serotypes grippotyphosa, ballum and ICF (frog isolate) were isolated from wild mammals and lower forms of life in the same vicinity.