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Arch Inst Pasteur Tunis ; 78(1-4): 3-9, 2001.
Artículo en Francés | MEDLINE | ID: mdl-14658233

RESUMEN

This study reports on the evaluation of two L. infantum specific DNA probes for the diagnosis of canine leishmaniasis. The probes presented very satisfying performances in terms of specificity (100%) and predictive value of the positive result (100%). However, their sensitivity (35.3%) and the clinical complexity of canine infections render their use difficult in epidemiological surveys of visceral leishmaniasis aiming at measuring the prevalence of the dog infection by L. infantum. The sensitivity of these tools has improved (66.7%) when dogs presenting patent leishmaniasis were considered. Such probes constitute appropriate tools to confirm suspected cases of leishmaniasis. Unlike the classical parasitological and serological tools, this kind of tools allows a concomitant detection and identification of the causative agent. Therefore, despite their low sensitivity, these probes can still be of importance in epidemiological investigations.


Asunto(s)
Sondas de ADN , ADN de Cinetoplasto/genética , Enfermedades de los Perros/diagnóstico , Leishmania infantum/genética , Leishmaniasis Visceral/diagnóstico , Leishmaniasis Visceral/veterinaria , Animales , Estudios de Casos y Controles , Sondas de ADN/genética , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/parasitología , Perros , Femenino , Humanos , Hibridación Genética , Leishmaniasis Visceral/epidemiología , Leishmaniasis Visceral/parasitología , Masculino , Sensibilidad y Especificidad , Túnez/epidemiología
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