RESUMEN
OBJECTIVE: To develop a new graft and to verify its technical feasibility, viability, and ability to reduce the risk of infection by interposition of an aortic segment between the airway and the prosthetic component of the graft. DESIGN: Experimental study. SETTING: Multicentre study, France. ANIMALS: 38 New Zealand rabbits. INTRODUCTION: A 1 cm aortic segment was obtained from the thoracic aorta of a rabbit and cryopreserved or stored at +4 degrees C. The aortic segment, surrounded by a ringed expanded polytetrafluoroethylene (ePTFE) prosthesis was used to replace 1 cm of cervical trachea in two groups of rabbits, either with cryopreserved or fresh aorta. MAIN OUTCOME MEASURES: Macroscopic, microbiological, and histological studies four months later. RESULTS: 12/20 animals in the cryopreserved group survived and 13/18 in those had had fresh aorta inserted. Partial or total necrosis of the aortic muscular layer was replaced by connective tissue, which was a guide for epithelialisation from the anastomoses. CONCLUSION: This new graft is worthy of further investigation, as it is technically feasible and easy to insert.
Asunto(s)
Aorta Torácica/trasplante , Prótesis Vascular , Tráquea/cirugía , Animales , Aorta Torácica/patología , Criopreservación , Rechazo de Injerto/patología , Necrosis , Politetrafluoroetileno , Complicaciones Posoperatorias , Conejos , Tráquea/patología , Trasplante Heterotópico/efectos adversosRESUMEN
This paper reviews the experience in the collection of 67 cord blood for use in sibling transplants. Details are given on the mean volumes collected, the numbers of nucleated cells, the CFU-GM and BFU-E obtained, and the incidence of collection failure.
Asunto(s)
Recolección de Muestras de Sangre/métodos , Sangre Fetal/citología , Trasplante de Células Madre Hematopoyéticas , Adulto , Femenino , Enfermedades Hematológicas/terapia , Humanos , Recién Nacido , Núcleo Familiar , Embarazo , Trasplante HomólogoRESUMEN
The authors studied the specificity and the sensitivity of serum anti-reticulin IgA type antibody determination for the diagnosis of coeliac disease in diabetic patients. They observed a frequency of 11.1% coeliac disease among a group of 54 type I juvenile diabetic patients. The search for serum antireticulin IgA type antibodies appears to be an efficient method for identifying patients with coeliac disease; it has a specificity of 98% and a sensitivity of 95%. This marker appears to be particularly well suited for the identification of asymptomatic forms, and also for surveillance of diet during the course of coeliac disease.
