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Biochim Biophys Acta ; 1393(2-3): 235-43, 1998 Aug 28.
Artículo en Inglés | MEDLINE | ID: mdl-9748601

RESUMEN

Human infection with the parasite Schistosoma mansoni is a relatively common occurrence in regions of South America and is associated with liver dysfunction and dyslipoproteinemia. Specifically, the activity of plasma lecithin:cholesterol acyltransferase (LCAT) activity is reduced, the concentration of plasma cholesterol esters falls, phospholipid concentrations are elevated and erythrocyte membranes become cholesterol enriched. Previous studies have utilized rodents (rats and mice) as experimental models to study the dyslipoproteinemia induced by S. mansoni infection. However, the plasma lipoprotein profiles in these animals is very different from humans and infection is not accompanied by decreases in LCAT activity or cholesterol enrichment of their erythrocyte membranes. Here we have evaluated the suitability of the marmoset Callithrix jacchus (sagüi) which is small and readily available in Brazil, as a potential animal model for the study of the dyslipoproteinemia of S. mansoni infections. The plasma lipoprotein compositions and distributions in sagüi, unlike rats or mice, approximate those of man with the LDL representing a major lipoprotein species. The molecular species of phospholipids, cholesterol esters and triglycerides present in sagüi plasma are also very similar to man, whereas those of rats and mice favor the longer chain more unsaturated species, Sagüi, like rodents, can be successfully infected with S. mansoni and after 60 days, this results in a 50% reduction in plasma LCAT activity, an 11% reduction in plasma cholesterol esters, an absolute increase of 46% in plasma phospholipids and an 18% increase in the cholesterol content of erythrocyte membranes. These changes are qualitatively and quantitatively very similar to those previously reported following human infections. Based upon these changes, and the observation that the plasma lipoprotein profile of sagüi and human is similar, we conclude that C. jacchus (sagüi) is an appropriate animal model for the study of dyslipoproteinemia associated with S. mansoni infections.


Asunto(s)
Glicoproteínas , Hipolipoproteinemias/complicaciones , Lipoproteínas/sangre , Esquistosomiasis mansoni/complicaciones , Animales , Callithrix , Proteínas Portadoras/sangre , Centrifugación por Gradiente de Densidad , Proteínas de Transferencia de Ésteres de Colesterol , Ésteres del Colesterol/sangre , Cromatografía de Gases , Modelos Animales de Enfermedad , Eritrocitos/metabolismo , Femenino , Humanos , Hipolipoproteinemias/sangre , Lipoproteínas/química , Masculino , Fosfatidilcolina-Esterol O-Aciltransferasa/sangre , Fosfolípidos/sangre , Fosfolípidos/química , Esquistosomiasis mansoni/sangre , Especificidad de la Especie , Triglicéridos/sangre
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