Systematic Structural Modification of Squaraine Dye for Near-Infrared Window One and Two Multiplexed In Vivo Imaging and Photothermal Therapy.

Due to the wide application of reporter gene-related visible/NIR-I bioluminescent imaging, multiplexed fluorescence imaging across visible/NIR-I/NIR-II has excellent potential in biomedical research. However, in vivo multiplexed imaging applications across those regions have rarely been reported due to the lack of proper fluorophores. Herein, nine squaraine dyes, which exhibit diverse adsorption and emission wavelengths, were synthesized. Among them, water-soluble SQ 710-5k and SQ 905 were found to have significant absorption differences, which allowed the tumor and lymph nodes to be identified. Then, for the first time, six-channel multiplexed fluorescence imaging across visible/NIR-I/II was achieved by coordination with reporter gene-related bioluminescent phosphors. Additional research revealed that SQ 710-5k exhibited higher-quality blood vessels and tumor imaging in NIR-II. H-aggregates SQ 905 demonstrated a high photothermal conversion efficiency for photothermal therapy. This study proposed an approach to creating small molecular dyes that coordinate with reporter gene-related bioluminescent phosphors for six-color fluorescence imaging.

[Changes of dental emergency cases in the first half from 2017 to 2020 in Shanghai Ninth People's Hospital].

PURPOSE: This study aimed to summarize and analyze dental emergence data and compare with the same period from 2017 to the first half of 2020, to provide a basis for improving the quality of dental emergency care and formulating rational allocation. METHODS: A total dental emergency cases from 2017 to the first half of 2020 were collected, statistical analysis was carried out according to patients' sex and age, chief complaints and diagnosis with SPSS 18.0 software package. RESULTS: The top three diseases in 2017 of dental emergency were maxillofacial trauma, facial infection and dental pulp disease. From 2018, most of the specialized patients were treated due to oral craniomaxillofacial injury , maxillofacial infection and tumors. The top three chief complaints for 3 consecutive years were facial swelling, toothache and dental trauma. The difference of emergency data within 4 years was statistically significant. CONCLUSIONS: There are a large number of patients which follow the regular pattern and a wide scope of disease types in dental emergency department. The total amount has a rising trend year by year. After divisions of dental emergency department are established, the patients' treatment is more timely, convenient and targeted, and the quality of medical service has been improving.

Lattice Disorder-Engineered Energy Splitting between Bright and Dark Excitons in CsPbBr3 Quantum Wires.

Excitons in nanostructured semiconductors often undergo strong electron-hole exchange interaction, resulting in bright-dark exciton splitting with the dark exciton usually being the lower energy state. This unfavorable state arrangement has become the major bottleneck for achieving high photoluminescence quantum yield (PLQY). However, the arrangement of dark and bright exciton states in lead halide perovskites is under intense debate due to the involvement of many complicated factors. We present here the first experimental evidence to demonstrate that the strain is a crucial factor in tuning the energy splitting of the bright and dark excitons, resulting in different PL properties.

[Effect of pulsed dye laser on inhibiting early scar after maxillofacial trauma].

PURPOSE: The study was designed to explore an effective method to control early scar after maxillofacial trauma and improve the satisfaction of clinical treatment. METHODS: Fifty skin lesions after maxillofacial trauma were divided into the experimental group and control group. Patients in the experimental group were treated with pulsed dye laser when taking out stitches, 15, 30 and 60 days later. Digital microscope photos were taken and lesion area was measured before and 3 months after laser irradiation. Adverse effects were recorded during and after each treatment as well. All patients were asked to rate their satisfaction at 3-month of follow-up. Statistical analysis was performed using SPSS 13.0 software package. RESULTS: The efficiency of the experimental group was 74% and 37 lesions were cured or significantly improved, while the efficiency rate was 22% in the control group. Area reduction of maxillofacial lesions before and after treatment between the two groups was significantly different (P＜0.05). Patients in the experimental group were highly satisfied with the final outcomes. No severe adverse events were observed. CONCLUSIONS: Pulsed dye laser is safe and effective in inhibiting early scar following maxillofacial trauma.

Edge optical scattering of two-dimensional materials.

Rayleigh scattering has shown powerful abilities to study electron resonances of nanomaterials regardless of the specific shapes. In analogy to Rayleigh scattering, here we demonstrate that edge optical scattering from two-dimensional(2D) materials also has the similar advantage. Our result shows that, in visible spectral range, as long as the lateral size of a 2D sample is larger than 2 µm, the edge scattering intensity distribution of the high-angle scattering in k space is nearly independent of the lateral size and the shape of the 2D samples. The high-angle edge scattering spectra are purely determined by the intrinsic dielectric properties of the 2D materials. As an example, we experimentally verify this feature in single-layer MoS2, in which A and B excitons are clearly detected in the edge scattering spectra, and the scattering images in k space and real space are consistent with our theoretical model. This study shows that the edge scattering is a highly practical and efficient method for optical studies of various 2D materials as well as thin films with clear edges.

A new LED device used for photodynamic therapy in treatment of moderate to severe acne vulgaris.

OBJECTIVE: This study investigated the efficacy and safety of a newly designed LED device used in photodiagnosis and photodynamic therapy of moderate to severe acne vulgaris in Chinese patients. METHODS: Forty-six patients with moderate to severe facial acne showing high degrees of fluorescence by ultraviolet light examination were illuminated during ALA-PDT with two wavelengths of light (543-548 nm, and 630±6 nm, respectively) after 2 h of incubation with ALA. Each patient received treatment once every 30 days for two or three sessions. Two independent investigators assigned an acne severity score at baseline, one week after each treatment, as well as 4, 8, and 12 weeks after the completion of treatment. Adverse effects were recorded during and after each treatment. All patients rated their satisfaction with the results of treatment at a 12-week follow up visit. RESULTS: The ALA-PDL treatment regimen showed an overall effectiveness rate of 89.13% (41/46 patients). Some degree of clinical efficacy was seen in 71.42%, 86.67%, and 95.83% of patients with grades IV, V, and VI acne, respectively, and the rate of clinical effectiveness increased with increasing acne severity. When compared with baseline scores, significant reductions in acne scores were obtained at 8, and 12 weeks after completion of treatment. Maximum efficacy was shown at the 12 week follow up. No severe adverse events were observed. CONCLUSION: ALA-PDT administered with the newly designed LED device was an effective treatment for moderate to severe acne vulgaris, and side effects were mild and reversible.