Cytauxzoon brasiliensis sp. nov. (Apicomplexa: Theileriidae), a new species infecting a little-spotted-cat (Leopardus tigrinus) (Carnivora: Felidae) from Brazil.

Cytauxzoon spp. have been detected in Brazil infecting mainly asymptomatic domestic cats and wild felids. However, the supposed genetic similarity with the pathogenic Cytauxzoon felis is questionable because it is based on analysis of short sequences of the 18S rRNA gene. Herein, we describe a novel Cytauxzoon species infecting an asymptomatic little-spotted-cat (Leopardus tigrinus) based on morphological, histopathological, and molecular analyses. The animal was attended presenting a history of a run-over with multiple traumas. Although the little-spotted-cat was stabilized, he died a few days later. Ring-shaped merozoites within erythrocytes were found on blood smears and in the abdominal effusion. In addition, schizonts were observed in histiocytes in the liver. Phylogenetic analyses based on both near-complete 18S rRNA and cytb genes positioned the obtained sequences in a unique clade, albeit closely related to Cytauxzoon felis from the USA. Genetic divergences ranging from 0.004 and 0.067-0.068 were found between the near-complete 18S rRNA and cytb sequences of Cytauxzoon sp. detected in the little-spotted-cat and C. felis, respectively. This study evidenced the circulation of a novel Cytauxzoon species, herein named Cytauxzoon brasiliensis sp. nov., in an asymptomatic wild felid species from Brazil. Further studies are necessary to identify Cytauxzoon species from domestic and wild felids in the country.

New insights on the phylogeography of Hepatozoon canis in Brazil.

This study aimed to molecularly characterize the Hepatozoon spp. infecting domestic and wild dogs in Brazil. A total of 22 whole blood samples tested positive for Hepatozoon spp., and five samples were sequenced for the 18S rDNA gene from H. canis after PCR amplification with four primer sets. Phylogenetic analysis using Bayesian inference showed that the three H. canis isolates from domestic dogs were not monophyletic; however, they were more closely related to each other than to other H. canis sequences. The isolate from the hoary fox (Lycalopex vetulus) was phylogenetically more distant. Two haplotype networks were constructed, identifying 10 haplotypes of H. canis in Brazil, with H10 constituting the largest group. It contains nine isolates, including three from domestic dogs. The H5 haplotype grouped the sequence of L. vetulus with two additional sequences from hosts Tapirus terrestris and L. vetulus, representing the sole haplotype with wild hosts. Bayesian analysis suggested the possible existence of two genetic groups of H. canis in Brazil, indicating gene flow of this agent within the country. These findings contribute valuable insights for a more comprehensive understanding of the molecular diversity of Hepatozoon spp. in Brazil and may help in the development of effective control measures.

Canine piroplasmids: Molecular detection and laboratory characterization in dogs from Brasilia, Brazil, with the first molecular evidence of dog exposure to a novel opossum-associated Babesia sp.

Canine piroplasmid infections can be caused by Babesia spp., Theileria spp. and Rangelia vitalii. In Brazil, canine babesiosis caused by Babesia vogeli is endemic and reported throughout the country. On the other hand, Rangeliosis caused by R. vitalii has only been described so far in the South and Southeast regions. Despite that, studies analyzing the laboratory and molecular characterization of these hemoprotozoa are still scarce. To investigate the occurrence, the laboratory features, the molecular characterization, and the diversity of piroplasmids from Midwestern Brazil, a survey was performed using blood samples obtained from 276 domestic dogs from Brasília, Federal District, Midwestern Brazil. A broad-range quantitative PCR (qPCR) targeting the mitochondrial large subunit ribosomal DNA (LSU4) was used to detect piroplasmid DNA. The overall molecular occurrence of piroplasmids was 11.2% (31/276), with 9.7% (27/276) of the sequences identified as Babesia vogeli (98-100% identity to B. vogeli isolate from the USA). Based on a partial 18S rRNA sequence pairwise alignment (-250 bp), 1.4% (4/276) of the sequences showed only 76.8% identity with B. vogeli but 100% identity with opossum-associated Babesia sp. (MW290046-53). These findings suggest the exposure of dogs from Brazil to a recently described Babesia sp. isolated from white-eared opossum. None of the analyzed dogs was positive for Theileria spp. or R. vitalii. Subsequently, all positive sequences were submitted to three additional PCR assays based on the 18S rRNA, cox-1, and cytb genes, aiming at performing a haplotype network analysis. Haplotype network using cox-1 sequences showed the presence of six different haplotypes of B. vogeli; one of them was shared with isolates from Brazil, the USA, and India. When including animals co-infected with other vector-borne diseases, piroplasmid-positive dogs had 2.3 times higher chance of having thrombocytopenia than the negative ones. The molecular results demonstrated that the compared Babesia vogeli sequences showed a low variability as well as evidence of exposure to a putative novel opossum-associated Babesia sp. in dogs from Midwestern Brazil.

Piroplasmid infection is not associated with clinicopathological and laboratory abnormalities in cats from Midwestern Brazil.

Feline piroplasmids include the genera Babesia spp., Cytauxzoon spp., and Theileria spp. In Brazil, there are few reports regarding these hemoprotozoans; however, clinicopathological and molecular data are scarce. This study aimed to characterize the clinical relevance of these parasites through hematological, biochemical, and molecular approaches. For this purpose, 166 cats from Brasilia, Federal District, Midwestern Brazil, were screened using a quantitative polymerase chain reaction (qPCR) for piroplasmids based on the LSU4 mitochondrial gene, which resulted in an overall prevalence of 36/166 (21.7%). Twelve of 166 samples (7.2%) were positive for C. felis, while 19/166 (11.4%) were positive for Babesia vogeli. No samples tested positive for Theileria spp. Babesia vogeli and Cytauxzoon spp. LSU4 sequences showed identities of 97-100% and 99.3%, respectively, to US isolates. The hematological and biochemical findings did not differ significantly between the cats that tested positive and negative for piroplasmids. Although the lack of abnormalities in clinical and laboratory parameters does not eliminate the possibility that these cats were sick and recovered, it may suggest that the Brazilian strain of Cytauxzoon spp. is not as pathogenic as that from the USA, despite the high molecular identity with North American isolates.

Molecular detection of piroplasmids in synanthropic rodents, marsupials, and associated ticks from Brazil, with phylogenetic inference of a putative novel Babesia sp. from white-eared opossum (Didelphis albiventris).

The order Piroplasmida encompasses tick-borne pathogens of veterinary and medical importance positioned in two main families: Babesiidae and Theileriidae. Even though previous studies carried out in Brazil recorded the occurrence of piroplasmid species circulating in small mammals, 18S RNA gene sequences were only partially sequenced, preventing the assessment of their phylogenetic positioning. The current study aimed to detect and characterize, using morphological, molecular, and bioinformatic approaches, piroplasmids from wild mammals and associated ticks sampled in Central-Western Brazil. Out of 67 Didelphis albiventris sampled, 22 (16.4%) were positive for piroplasmids by PCR. In contrast, none of the 48 small rodents and 14 capybaras (Hydrochoerus hydrochaeris) was PCR-positive. Four Amblyomma dubitatum ticks-one from Rattus rattus, one from H. hydrochaeris, and two from D. albiventris-out of 114 Amblyomma spp. DNA samples were positive for piroplasmids by PCR. The phylogenetic inference performed using the near-complete 18S rRNA gene positioned the putative novel piroplasmid species detected in D. albiventris and associated A. dubitatum ticks near to Babesia sensu lato clade (Western group-cluster III) and distant from the Australian marsupial-associated piroplasms. Phylogenetic inferences based on two additional molecular markers, namely hsp-70 and cox-1, supported the near-complete 18S rRNA gene phylogenetic inference. Finally, the partial 18S rRNA gene sequences detected in ticks from rodents (R. rattus and H. hydrochaeris) showed 97.2-99.4% identity with the Piroplasmida previously detected in a capybara from Brazil, raising evidence that a still uncharacterized piroplasmid species has been identified in the capybara, the largest rodent species from South America.

Eurytrema coelomaticum natural infection in small ruminants: a neglected condition.

Pancreatic eurytrematosis (PE) is an under diagnosed and neglected parasitosis in goats and sheep in the Americas. Clinical and pathological features of PE are not well defined in small ruminants worldwide. Natural cases of PE in small ruminants were detected in the Federal District, Brazil. A survey of necropsy records, including epidemiological and clinicopathological data, in goats and sheep was conducted. Most cases of PE occurred during the rainy season in adult females, with an incidence of 12.9% in goats and 0.8% in sheep. Clinical signs varied from asymptomatic infections to anorexia, lethargy, weakness, marked weight loss and death in some goats. Overall, most cases of PE in goats and sheep were incidental necropsy findings with minor pancreatic lesions. Three goats, however, showed severe chronic pancreatitis, dilation of major pancreatic ducts with numerous trematodes present and marked abdominal fat necrosis. Morphological and molecular characterization of flukes detected Eurytrema coelomaticum. Our findings shed light on the prevalence of E. coelomaticum infections in small ruminants in the region and highlight the possibility of severe and lethal cases in goats. PE must be further investigated in small ruminant populations in relevant livestock production regions of the Americas.

Distribuição espacial e temporal da fauna de invertebrados bentônicos na APA do município de Coqueiral, MG, com ênfase em Odonata / Spatial and temporal distribution of benthic invertebrates fauna in APA of the municipality Coqueiral, MG, with emphasis on Odonata

The benthic invertebrates are a diverse group of organisms that inhabit both lentic and lotic environments and have an important role in the aquatic ecosystem dynamics, and its use for the evaluation of impacts on aquatic environments, widely recommended. Among the benthic invertebrates, highlight the Odonata use for verification of environmental quality. The present study had as objective to know the spatial and temporal distribution of benthic invertebrates fauna in the stream ofErmo, in the APA of the municipality - Coqueiral, MG, with emphasis on Odonata, and infer on the local environmental conditions. Samples were collected during the rainy season (February, 2007) and dry (August, 2007) at seven sites along the stream. First were measured and recorded abiotic variables: water temperature with a thermometer, depth using a graduated ruler and the dissolved oxygen content with oximeter. Later sediments were collected for analysis of benthic invertebrates, using a Surber sampler. For Anisoptera were identified: Dythemis, Nannothemis, Octogomphus and Progomphus and for Zygoptera: Argia. The Jaccard analysis results showed a higher similarity between points 2 and 3 grouped due to the similarities of physic-chemical parameters, mainly temperature and dissolved oxygen recorded in the stream and a separation of the points 5 and 7. Studies about Odonata fauna are needed due to the great potential bioindicator that these organisms have, mainly because of its wide distribution.

Os invertebrados bentônicos constituem um grupo diversificado de organismos que habitam tanto ambientes lênticos quanto lóticos e exercem importante papel na dinâmica do ecossistema aquático, sendo seu uso para a avaliação de impactos em ambientes aquáticos, amplamente recomendado. Dentre os invertebrados bentônicos, destaca-se o uso de Odonata para a verificação da qualidade ambiental. O presente estudo teve como objetivo conhecer a distribuição espacial e temporal da fauna de invertebrados bentônicos no córrego do Ermo, na APA do município de Coqueiral, MG com ênfase em Odonata, bem como inferir sobre as condições ambientais locais. As amostragens foram realizadas na estação chuvosa (Fevereiro, 2007) e seca (Agosto,2007), em sete pontos ao longo do córrego. Primeiramente foram medidas e registradas as variáveis abióticas: a temperatura da água com um termômetro, a profundidade utilizando-se uma régua graduada e o teor de oxigênio dissolvido com oxímetro.Posteriormente foram coletados sedimentos para análise dos invertebrados bentônicos, com auxílio de um amostrador tipo Surber. Foram identificados para Anisoptera: Dythemis, Nannothemis, Octogomphus e Progomphus e para Zygoptera: Argia. Osresultados da análise de Jaccard evidenciaram uma maior similaridade entre os pontos 2 e 3, agrupados devido às semelhanças dos parâmetros físico-químicos, principalmente temperatura e oxigênio dissolvido registrados no córrego e uma separação dos pontos 5 e 7. Estudos sobre a fauna de Odonata são necessários devido ao grande potencial bioindicador que esses organismos possuem, principalmente por sua ampla distribuição.

Distribuição espacial e temporal da fauna de invertebrados bentônicos na APA do município de Coqueiral, MG, com ênfase em Odonata / Spatial and temporal distribution of benthic invertebrates fauna in APA of the municipality Coqueiral, MG, with emphasis on Odonata

The benthic invertebrates are a diverse group of organisms that inhabit both lentic and lotic environments and have an important role in the aquatic ecosystem dynamics, and its use for the evaluation of impacts on aquatic environments, widely recommended. Among the benthic invertebrates, highlight the Odonata use for verification of environmental quality. The present study had as objective to know the spatial and temporal distribution of benthic invertebrates fauna in the stream ofErmo, in the APA of the municipality - Coqueiral, MG, with emphasis on Odonata, and infer on the local environmental conditions. Samples were collected during the rainy season (February, 2007) and dry (August, 2007) at seven sites along the stream. First were measured and recorded abiotic variables: water temperature with a thermometer, depth using a graduated ruler and the dissolved oxygen content with oximeter. Later sediments were collected for analysis of benthic invertebrates, using a Surber sampler. For Anisoptera were identified: Dythemis, Nannothemis, Octogomphus and Progomphus and for Zygoptera: Argia. The Jaccard analysis results showed a higher similarity between points 2 and 3 grouped due to the similarities of physic-chemical parameters, mainly temperature and dissolved oxygen recorded in the stream and a separation of the points 5 and 7. Studies about Odonata fauna are needed due to the great potential bioindicator that these organisms have, mainly because of its wide distribution.(AU)

Os invertebrados bentônicos constituem um grupo diversificado de organismos que habitam tanto ambientes lênticos quanto lóticos e exercem importante papel na dinâmica do ecossistema aquático, sendo seu uso para a avaliação de impactos em ambientes aquáticos, amplamente recomendado. Dentre os invertebrados bentônicos, destaca-se o uso de Odonata para a verificação da qualidade ambiental. O presente estudo teve como objetivo conhecer a distribuição espacial e temporal da fauna de invertebrados bentônicos no córrego do Ermo, na APA do município de Coqueiral, MG com ênfase em Odonata, bem como inferir sobre as condições ambientais locais. As amostragens foram realizadas na estação chuvosa (Fevereiro, 2007) e seca (Agosto,2007), em sete pontos ao longo do córrego. Primeiramente foram medidas e registradas as variáveis abióticas: a temperatura da água com um termômetro, a profundidade utilizando-se uma régua graduada e o teor de oxigênio dissolvido com oxímetro.Posteriormente foram coletados sedimentos para análise dos invertebrados bentônicos, com auxílio de um amostrador tipo Surber. Foram identificados para Anisoptera: Dythemis, Nannothemis, Octogomphus e Progomphus e para Zygoptera: Argia. Osresultados da análise de Jaccard evidenciaram uma maior similaridade entre os pontos 2 e 3, agrupados devido às semelhanças dos parâmetros físico-químicos, principalmente temperatura e oxigênio dissolvido registrados no córrego e uma separação dos pontos 5 e 7. Estudos sobre a fauna de Odonata são necessários devido ao grande potencial bioindicador que esses organismos possuem, principalmente por sua ampla distribuição.(AU)