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Clin Microbiol Infect ; 25(10): 1289.e5-1289.e8, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31229597

RESUMEN

OBJECTIVES: Staphylococcus argenteus has been increasingly reported since the species was defined as a novel staphylococcal species in 2015. This study aims to investigate genetic epidemiological links and antimicrobial susceptibilities of methicillin-resistant S. argenteus isolates recovered in Stockholm. METHODS: Sixteen methicillin-resistant S. argenteus isolates were identified from a collection of methicillin-resistant Staphylococcus aureus in Stockholm 2007-2018, by using whole-genome sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The genomes of the isolates were investigated by pulsed-field gel electrophoresis, single-nucleotide polymorphism (SNP)-based phylogeny, k-mer analysis, core-genome multi-locus sequence typing (cgMLST), resistance traits and virulence factors. The MICs of 19 antimicrobial agents for each isolate were determined by using the broth microdilution method. RESULTS: Of the 16 isolates, seven, seven and two isolates were assigned to ST1223, ST2250 and ST2793, respectively, with the S. aureus MLST-scheme. Analyses based on SNPs and cgMLST revealed a likely clonal spread of methicillin-resistant S. argenteus in 2007. Four isolates were found to be resistant to non-ß-lactams in antimicrobial susceptibility testing. CONCLUSIONS: A transmission event of methicillin-resistant S. argenteus in family was identified by this study. Among our limited number of isolates, non-ß-lactam resistance was detected, which highlights the necessity of a continued surveillance on this emerging pathogen. S. argenteus could be correctly identified by MALDI-TOF MS with the updated database, enabling its detection also in clinical laboratories.


Asunto(s)
Resistencia a la Meticilina , Infecciones Estafilocócicas/epidemiología , Infecciones Estafilocócicas/transmisión , Staphylococcus/efectos de los fármacos , Staphylococcus/aislamiento & purificación , Antibacterianos/farmacología , Transmisión de Enfermedad Infecciosa , Electroforesis en Gel de Campo Pulsado , Salud de la Familia , Genotipo , Humanos , Pruebas de Sensibilidad Microbiana , Epidemiología Molecular , Tipificación de Secuencias Multilocus , Polimorfismo de Nucleótido Simple , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Staphylococcus/clasificación , Staphylococcus/genética , Suecia/epidemiología , Secuenciación Completa del Genoma
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