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1.
J Microsc ; 233(2): 269-74, 2009 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-19220693

RESUMEN

We have developed a stroboscopic near-field optical microscope for observation of biological specimens and observed glycerinated muscles before and after muscle contraction with the developed system. In the system, the optical field distribution localized near the specimen is recorded as the surface topographic distribution of a photosensitive film surface. Our system is very useful for observing living biological specimens with high resolutions, because it is possible to get stroboscopic image by using a photosensitive film as detecting optical distributions instead of a scanning of probes. We have succeeded in observing inner structures of muscle cells with sub-wavelength resolution and achieved higher contrast than an ordinary optical microscope.


Asunto(s)
Células Musculares/ultraestructura , Contracción Muscular/fisiología , Animales , Glicerol , Rayos Láser , Microscopía/instrumentación , Microscopía/métodos , Células Musculares/fisiología
2.
J Microsc ; 202(Pt 1): 162-71, 2001 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-11298887

RESUMEN

We have developed a non-optically probing near-field microscope with illumination of total internal reflection. Because the illumination light does not pass through the specimens, it is possible to observe thick specimens or highly absorptive materials. It reduces the background noise because the decay length of the evanescent wave is a few hundred nanometres. We found that although in the total internal reflection illumination system the light passed through the photosensitive film and illuminated the specimen, it did not affect the photosensitive film severely and did not limit the resolution. The imaging properties of reflection illumination and transmission illumination are analysed using a finite-differential time domain method.

3.
Opt Lett ; 25(14): 1028-30, 2000 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-18064262

RESUMEN

A simple technique for fabrication of nonlinear gratings in polymer films, based on simultaneous embossing and poling, is proposed and demonstrated. A master grating consisting of a metal electrode with a dielectric die was fabricated and used for repeated embossing of the grating structures into nonlinear optical polymers at elevated temperatures. At the same time, we applied high voltage to the polymer films to induce second-order nonlinearity. The grating profile and the nonlinearity were estimated, as well as the mass productivity of nonlinear gratings.

4.
Appl Opt ; 39(30): 5632-7, 2000 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-18354560

RESUMEN

A simple fabrication technique for nonlinear polymeric optical waveguide patterns is introduced based on the two-beam interference method. We determined that the second-order nonlinearity of poled polymer films is erased by single-pulse ultraviolet (UV) laser irradiation. The erasure mechanism for second-order nonlinearity is discussed. To form a periodic structure in an optical polymer waveguide, two types of optical configuration of two-beam interference were arranged, and a single-pulse UV laser was exposed directly onto poled films. We prove that this method provides a simple way to fabricate volume-type and ridge-type periodically poled structures, i.e., chi(2) gratings, from the submicrometer to the millimeter range.

5.
Appl Opt ; 38(29): 6201-4, 1999 Oct 10.
Artículo en Inglés | MEDLINE | ID: mdl-18324144

RESUMEN

We present a polarization-multiplexed optical memory with urethane-urea copolymers. The side chains of the urethane-urea copolymers induce cis-trans isomerization by illumination of blue or green light, and they align perpendicular to the linear polarization of the illuminated light, thus producing optical anisotropy. We found that the material showed selective anisotropy for the particular direction that was perpendicular to that of the recording beam polarization. By use of the anisotropic property three different data pages were multiplexed at the same spot of the medium. Erasure of the recorded bit data is also demonstrated.

6.
Opt Lett ; 23(22): 1781-3, 1998 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-18091913

RESUMEN

We present a multilayered optical memory for use in reading data with a confocal reflection microscope system. We use a recording medium in which photosensitive thin films and nonphotosensitive transparent films are stacked alternately. Since the photosensitive films are thinner than the depth of focus of the recording beam, the spatial frequency distribution of the recorded bit data is extended in the axial direction. The extended distribution overlaps the coherent optical transfer function of the reflection-type confocal microscope. Urethane-urea copolymer film is used as a photosensitive material. The recording and reading of two layers are demonstrated.

7.
Appl Opt ; 37(7): 1213-9, 1998 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-18268706

RESUMEN

A simple fabrication technique of nonlinear optical structures for use with dye-doped polymer is described. Polymethylmethacrylate, U-100 polymer, and polystyrene were used as the host matrices to fabricate the nonlinear optical waveguide. The periodically poled nonlinear optical polymer structures and ridge-type channel structures were fabricated by direct electron-beam irradiation. The electron beam with 25 kV of energy was exposed directly onto the polymer films containing the nonlinear optical chromophores. We can also demonstrate the fabrication technique of the domain-inverted grating of dye-doped polystyrene film.

8.
Opt Lett ; 22(18): 1424-6, 1997 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-18188258

RESUMEN

We report on self-adaptive spatial filtering by combination of the self-adaptive polarization-rotation property of Disperse Red1-doped polymers with low glass-transition-temperature and Fourier-transform operation. Our system can be operated with only an action beam with object information and without a probe beam for image readout. Edge enhancement by the action beam-probe beam is demonstrated as an example of the system's application to spatial filtering.

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