RESUMEN
The aim of the present study was to investigate the presence of extended-spectrum beta-lactamases (ESBL) in Klebsiella pneumoniae isolated at the "Hospital de Niños de Córdoba". The strains were collected from inpatients between January 1996 and July 2000. A total of 150 ESBL producer isolates were detected. During 1996 the prevalence of ESBL producer K. pneumoniae was 20%, but since 1998 the values have increased to approximately 60%. Phenotypic analysis such as isoelectric point (pl) and antibiotyping performed in 32 randomly selected isolates showed two different enzyme profiles: 81% had ESBL with pl = 7.9 and preferential activity against cefotaxime, while 19% showed ESBL with pl = 5.4 and preferential activity against ceftazidime. No isolates resistant to imipenem or ciprofloxacin were detected. Susceptibility to other antimicrobial agents varied, but resistance to gentamicin was strongly associated with ESBL producer isolates. Resistance determinants could be transferred to Escherichia coli by conjugation assays.
Asunto(s)
Proteínas Bacterianas/aislamiento & purificación , Infección Hospitalaria/microbiología , Farmacorresistencia Bacteriana Múltiple , Infecciones por Klebsiella/microbiología , Klebsiella pneumoniae/enzimología , Resistencia betalactámica , beta-Lactamasas/aislamiento & purificación , Antibacterianos/metabolismo , Antibacterianos/farmacología , Argentina/epidemiología , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Niño , Conjugación Genética , Infección Hospitalaria/epidemiología , Farmacorresistencia Bacteriana Múltiple/genética , Hospitales Pediátricos , Humanos , Punto Isoeléctrico , Infecciones por Klebsiella/epidemiología , Klebsiella pneumoniae/efectos de los fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/aislamiento & purificación , Fenotipo , Prevalencia , Estudios Retrospectivos , Especificidad por Sustrato , Resistencia betalactámica/genética , beta-Lactamasas/genética , beta-Lactamasas/metabolismo , beta-LactamasRESUMEN
The aim of the present study was to investigate the presence of extended-spectrum beta-lactamases (ESBL) in Klebsiella pneumoniae isolated at the [quot ]Hospital de Niños de Córdoba[quot ]. The strains were collected from inpatients between January 1996 and July 2000. A total of 150 ESBL producer isolates were detected. During 1996 the prevalence of ESBL producer K. pneumoniae was 20
, but since 1998 the values have increased to approximately 60
. Phenotypic analysis such as isoelectric point (pl) and antibiotyping performed in 32 randomly selected isolates showed two different enzyme profiles: 81
had ESBL with pl = 7.9 and preferential activity against cefotaxime, while 19
showed ESBL with pl = 5.4 and preferential activity against ceftazidime. No isolates resistant to imipenem or ciprofloxacin were detected. Susceptibility to other antimicrobial agents varied, but resistance to gentamicin was strongly associated with ESBL producer isolates. Resistance determinants could be transferred to Escherichia coli by conjugation assays.
RESUMEN
To assess the effect of age at the onset of menopause over the skeleton we have determined the age and cause of menopause and bone mineral density (BMD), by dual and single photon absorptiometry of the lumbar spine, the proximal femur and the radius shaft on 1050 osteoporotic women (suffering with at least one atraumatic vertebral fracture). The mean +/- 1 S.D. menopausal age was 47.1 +/- 7.6 years. The onset of menopause occurred prior to age 45 in 22% (premature), after age 52 in 9% (late), and between 45 and 52 years of age in 69% (normal menopausal age). When the osteoporotic women were categorized into three groups according to the age of menopause, those osteoporotic with premature menopause had a significantly greater frequency of hip fractures, a significantly lower age, weight and BMD over the spine, proximal femur and radius shaft compared with those of normal menopausal age. In turn, patients with late menopause had a significantly increased weight and BMD over the spine. These findings indicate that among patients with vertebral osteoporosis those women with premature menopause had a more severe bone loss and a significantly greater frequency of hip fractures.