RESUMEN
Helicobacter species have been reported in animals, some of which are of zoonotic importance. This study aimed to detect Helicobacter species among human and animal samples using conventional PCR assays and to identify their zoonotic potentials. Helicobacter species was identified in human and animal samples by genus-specific PCR assays and phylogenetic analysis of partial sequencing of the 16S ribosomal RNA gene. The results revealed that Helicobacter species DNA was detected in 13 of 29 (44.83%) of the human samples. H. pylori was identified in 2 (15.38%), and H. bovis was detected in 4 (30.77%), whereas 7 (53.85%) were unidentified. H. bovis and H. heilmannii were prevalent among the animal samples. Phylogenetic analysis revealed bootstrapping of sequences with H. cinaedi in camel, H. rappini in sheep and humans, and Wollinella succinogenes in humans. In conclusion, the occurrence of non-H. pylori infections among human and animal samples suggested zoonotic potentials.
Asunto(s)
Enfermedades de los Gatos/microbiología , Enfermedades de los Perros/microbiología , Infecciones por Helicobacter/veterinaria , Helicobacter/genética , Helicobacter/aislamiento & purificación , Zoonosis/microbiología , Animales , Enfermedades de los Gatos/epidemiología , Gatos , ADN Bacteriano/genética , Enfermedades de los Perros/epidemiología , Perros , Heces/microbiología , Genoma Bacteriano , Helicobacter/clasificación , Infecciones por Helicobacter/diagnóstico , Infecciones por Helicobacter/microbiología , Hospitales Veterinarios , Humanos , Filogenia , Reacción en Cadena de la Polimerasa , Saliva/microbiología , Zoonosis/epidemiologíaAsunto(s)
Toxinas Bacterianas/sangre , Absceso Encefálico/diagnóstico , Infecciones Comunitarias Adquiridas/diagnóstico , Exotoxinas/sangre , Leucocidinas/sangre , Resistencia a la Meticilina , Infecciones Estafilocócicas/diagnóstico , Staphylococcus aureus/aislamiento & purificación , Absceso Encefálico/sangre , Absceso Encefálico/microbiología , Infecciones Comunitarias Adquiridas/sangre , Infecciones Comunitarias Adquiridas/microbiología , Egipto , Humanos , Masculino , Persona de Mediana Edad , Infecciones Estafilocócicas/sangre , Infecciones Estafilocócicas/microbiologíaRESUMEN
A polymerase chain reaction (PCR) was developed from sequencing data generated from a specific target band that is unique for Aspergillus fumigatus DNA digested with EcoR1. The target band was detected through Southern blot hybridization of a non-radioactive probe labelled with DIG-dUTP and DNAs of different aspergilli. The DNA of the target band was purified, concentrated and subjected to sequencing. The size of the sequenced band was approximately 445 bp. One pair of primers was designed and synthesized from the sequencing data of the band. The oligonucleotide primers were specific in amplifying an identical band of A. fumigatus in a population mix containing DNAs of different Aspergillus spp.; Pencillium spp.; yeasts; bacterial and viral organisms that are commonly encountered in clinical specimens of respiratory origin. The reaction proved highly sensitive and as little as 0.0001 microgram of A. fumigatus DNA was detected in the reaction.
Asunto(s)
Aspergilosis/diagnóstico , Aspergillus fumigatus/aislamiento & purificación , ADN de Hongos/análisis , Secuencia de Aminoácidos , Animales , Aspergillus fumigatus/genética , Southern Blotting , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/métodosRESUMEN
The synthesis of certain 1.5-benzodiazepinediones, some of their oxygen-free analogues and a number of the 7-nitro derivatives is described. Condensation of 3.4-diaminopyridine with diethylmalonate instead of affording the expected pyridodiazepine, yielded an imidazopyridine, the structure of which was inferred from spectral data Nevertheless, the pyridodiazepine was obtained by condensing the diamino-heterocycle with malonyl dichloride.
