Human amniotic membrane-derived stromal cells (hAMSC) interact depending on breast cancer cell type through secreted molecules.

Human amniotic membrane-derived stromal cells (hAMSC) are candidates for cell-based therapies. We examined the characteristics of hAMSC including the interaction between hAMSC and breast cancer cells, MCF-7, and MDA-MB-231. Human amniotic membrane-derived stromal cells showed typical MSC properties, including fibroblast-like morphology, surface antigen expression, and mesodermal differentiation. To investigate cell-cell interaction via secreted molecules, we cultured breast cancer cells in hAMSC-conditioned medium (hAMSC-CM) and analyzed their proliferation, migration, and secretome profiles. MCF-7 and MDA-MB-231 cells exposed to hAMSC-CM showed increased proliferation and migration. However, in hAMSC-CM, MCF-7 cells proliferated significantly faster than MDA-MB-231 cells. When cultured in hAMSC-CM, MCF-7 cells migrated faster than MDA-MB-231 cells. Two cell types showed different profiles of secreted factors. MCF-7 cells expressed much amounts of IL-8, GRO, and MCP-1 in hAMSC-CM. Human amniotic membrane-derived stromal cells interact with breast cancer cells through secreted molecules. Factors secreted by hAMSCs promote the proliferation and migration of MCF-7 breast cancer cells. For much safe cell-based therapies using hAMSC, it is necessary to study carefully about interaction between hAMSC and cancer cells.

The effects of squat exercises in postures for toilet use on blood flow velocity of the leg vein.

[Purpose] The purpose of this study was to identify the effects of squat exercises performed in toilet-using postures on the blood flow velocity of the lower extremities for the prevention of deep vein thrombosis. [Subjects] The subjects were 28 students who were attending B University in Cheonan. They were divided into a group of 14 subjects of sitting toilet users and a group of 14 subjects of squat toilet users. [Methods] The subjects performed squat exercises in different toilet-using postures and we investigated the changes in blood flow velocity. [Results] The variations in blood flow velocities before and after the exercises showed significant differences in both groups but the differences between the two groups were not significant. [Conclusion] Based on the results of this study, we consider squat exercises are effective at improving the variation in lower-extremity blood flow velocity when using a toilet.