Tunnel expansion after hamstring anterior cruciate ligament reconstruction with 1-incision EndoButton femoral fixation.

In this study, we compared a study group of 20 patients who underwent anterior cruciate ligament (ACL) reconstruction with hamstring autografts using a 1-incision endoscopic technique verses a control group of 20 patients using a 2-incision technique. The patient groups were compared based on increase of bone tunnel diameter seen radiographically, physical examination, and arthrometer measurements. The 1-incision technique differed from the 2-incision technique in 2 ways: an EndoButton femoral fixation system and drilling of the femoral tunnel through the tibial tunnel (transtibial). This study shows that the majority of tunnel diameter measurements for the 1-incision ACL reconstruction technique were greater than those of the 2-incision ACL reconstruction technique using autologous hamstring tendons, at both 3 and 12 months of follow-up. Between 3 and 12 months follow-up, there was no statistical differences in tunnel enlargement between the 2 groups of patients. The measured tunnel enlargement, therefore, would have occurred before the 3-month follow-up. There was no significant difference in the Lachman or arthrometer testing in either group of patients at the termination of this study. This indicates that, although tunnel expansion is significant, the increased expansion is not related to increased knee laxity in the first year.

Misexpression of nautilus induces myogenesis in cardioblasts and alters the pattern of somatic muscle fibers.

nautilus (nau), one member of the myogenic regulatory family of bHLH-encoding genes, is expressed in a subset of muscle precursors and differentiated fibers in the Drosophila embryo. To elucidate the role of nautilus in myogenesis, we have misexpressed it using the GAL4-targeted system. We find that ectopic expression results in lethality throughout Drosophila development. We analyzed the effects of embryonic expression in mesodermal tissues that include the cardioblasts of the dorsal vessel as well most, if not all, of the presumptive somatic muscle precursors. Immunohistochemical staining for muscle MHC revealed abnormalities that include an absence of cardial cells, coincident with the appearance of novel muscle fibers adjacent to the dorsal vessel. Moreover, many cardioblasts express increased levels of muscle-specific genes such as myosin, actin 57B, and Mlp60A, a protein that is restricted to the somatic, visceral, and pharyngeal muscles. These data suggest that the missing cardial cells have been transformed into cells with properties similar to those of the somatic muscles. In addition, ubiquitous expression of nautilus in somatic muscle cells of these embryos resulted in muscle pattern defects. Specifically, muscles that do not normally express nautilus were frequently absent, and novel fibers were observed in positions reminiscent of nau-expressing muscles. These data imply that nautilus can alter the developmental program of muscle precursors. In summary, we suggest that nautilus induces myogenic differentiation in vivo when ectopically expressed in developing cardioblasts and may affect the myogenic differentiation program of specific muscle fibers.

Embryonic development of the larval body wall musculature of Drosophila melanogaster.

The somatic, or body wall, muscles of the larva of Drosophila melanogaster are composed of an elaborate pattern of segmentally repeating fibers that form during embryogenesis. The primordia of these muscles progress from morphologically indistinct mesodermal cells to multinucleate syncytia with unique characteristics that include shape, size, location and attachment to the epidermis. Although relatively little is known about the development of the musculature and the mechanisms by which this elaborate pattern is achieved, recent progress has begun to reveal key players in this process.