A review of starch/polyvinyl alcohol (PVA) blend film: A potential replacement for traditional plastic-based food packaging film.

In recent years, the development of environmentally friendly packaging materials using biodegradable polymers has emerged as a key challenge for scientists and consumers in response to resource depletion and environmental issues caused by plastic packaging materials. Starch and polyvinyl alcohol (PVA) are being recognized as excellent candidates for producing biodegradable food packaging films. Polymer blending has emerged as a practical approach to overcome the limitations of biopolymer films by developing films with unique properties and enhancing overall performance. This review briefly introduces the molecular structure and properties of starch and PVA, summarizes the common preparation methods and properties of starch/PVA blend films, and focuses on different strategies used to enhance starch/PVA blend films, including nanoparticles, plant extracts, and cross-linking agents. Additionally, this study summarizes the application of starch/PVA blend films as active and smart packaging in food preservation systems. This study demonstrates that starch and PVA blends have potential in manufacturing biodegradable food films with excellent properties due to their excellent compatibility and intermolecular interactions, and can be used as packaging films for a variety of foods to extend their shelf life.

Advances in sustainable food packaging applications of chitosan/polyvinyl alcohol blend films.

Researchers are addressing environmental concerns related to petroleum-based plastic packaging by exploring biopolymers from natural sources, chemical synthesis, and microbial fermentation. Despite the potential of individual biopolymers, they often exhibit limitations like low water resistance and poor mechanical properties. Blending polymers emerges as a promising strategy to overcome these challenges, creating films with enhanced performance. This review focuses on recent advancements in chitosan/polyvinyl alcohol (PVA) blend food packaging films. It covers molecular structure, properties, strategies for performance improvement, and applications in food preservation. The blend's excellent compatibility and intermolecular interactions make it a promising candidate for biodegradable films. Future research should explore large-scale thermoplastic technologies and investigate the incorporation of additives like natural extracts and nanoparticles to enhance film properties. Chitosan/PVA blend films offer a sustainable alternative to petroleum-based plastic packaging, with potential applications in practical food preservation.

Determination of aflatoxin M1 and ochratoxin A in breast milk in rural centers of Yazd, Iran: Exposure assessment and risk characterization.

Breast milk (BM) is considered as the best source of nutrition which could have prevention effects on various diseases in the first years of a child. Along with nutritive compounds, presence of contaminants such as mycotoxins in BM could be transmitted into neonate. The aim of this study was to determine the occurrence, levels, and factors associated with the presence of aflatoxin M1 (AFM1) and ocratoxin a (OTA) in BM samples of nursing mothers in rural centers of Yazd, Iran. The presence and average AFM1 and OTA concentration in 72 BM samples was measured by competitive ELISA. The demographic and diet parameters of nursing mothers were collected by a questionnaire and were analyzed using SPSS 18 software. AFM1 and OTA were detected in 63 (87.5%) and 47 (65.2%) samples with the mean concentration levels of 19.46 ± 13.26 ng/L (ranges from 5.1 to 53.9) and 200 ± 160 ng/L (ranges from 100 to 2460), respectively. Of these, 32 samples (50.7%) for AFM1 and 23 samples (48.9%) for OTA had values exceeding the limit set by the European Union regulation for infant foods (25 ng/L for AFM1 and 500 ng/L for OTA). It was also found that the risk of AFM1 and OTA occurrence in BM increased significantly with the consumption of beans, bread, cereals, fruit juice and crackers, and cream, respectively. This study showed that the estimated daily intake for AFM1 and OTA by 1 month of age infants was 2.7 and 28.5 ng/kg bw/day, respectively, while, as the age of the infant increased, the values were lower and close to 0.9 and 9.9 ng/kg bw/day for AFM1 and OTA in 12 months of age infants, respectively. The high occurrence and noticeable levels of AFM1 and OTA detected in this study indicated that some infants receive undesirable exposures to AFM1 and OTA with breast milk. Therefore, it is recommended that mothers are advised to avoid certain foods during pregnancy and breastfeeding that are likely sources of mycotoxins.

Encapsulation and delivery systems of cinnamon essential oil for food preservation applications.

Food safety threats and deterioration due to the invasion of microorganisms has led to economic losses and food-borne diseases in the food industry; so, development of natural food preservatives is urgently needed when considering the safety of chemically synthesized preservatives. Because of its outstanding antioxidant and antibacterial properties, cinnamon essential oil (CEO) is considered a promising natural preservative. However, CEO's low solubility and easy degradability limits its application in food products. Therefore, some encapsulation and delivery systems have been developed to improve CEO efficiency in food preservation applications. This work discusses the chemical and techno-functional properties of CEO, including its key components and antioxidant/antibacterial properties, and summarizes recent developments on encapsulation and delivery systems for CEO in food preservation applications. Since CEO is currently added to most biopolymeric films/coatings (BFCs) for food preservation, most studies have shown that encapsulation systems can improve the food preservation performance of BFCs containing CEOs. It has been confirmed that various delivery systems could improve the stability and controlled-release properties of CEO, thereby enhancing its ability to extend the shelf life of foods. These encapsulation techniques include spray drying, emulsion systems, complex coacervation (nanoprecipitation), ionic gelation, liposomes, inclusion complexation (cyclodextrins, silica), and electrospinning.

Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application.

Carbon dots (CDs) were manufactured with banana peels using a hydrothermal method (200 °C for 6 h). The synthesized CDs were spherical particles with a size of 1-3 nm having carboxyl groups and amine groups on the surface. CDs have been impregnated into chitosan/gelatin films to synthesize multifunctional packaging films. The composite film showed a slight decrease in transparency but a significant increase in UV protection properties. The fabricated film displayed strong antioxidant efficacy showing >74 % DPPH and 99 % ABTS radical scavenging potential. The film also unveiled substantial antibacterial activity against the foodborne pathogenic bacteria, Listeria monocytogenes, fully eliminating the growth of these bacteria within 6 h of exposure. The chitosan/gelatin film containing CD was used for minced meat packaging, and the film delayed bacterial growth (< 1 Log CFU/g after 24 h) and maintained the meat color even after 24 h of storage at 20 °C. The CD-added chitosan/gelatin functional film has a high probability of application in active food packaging, especially for extending the shelf life of packaged meat and maintaining its aesthetic quality.

New opportunities and advances in quercetin-added functional packaging films for sustainable packaging applications: a mini-review.

Recently, research on functional packaging films and their application to food preservation has been actively conducted. This review discusses recent advances and opportunities for using quercetin in developing bio-based packaging films for active food packaging. Quercetin is a plant-based yellow pigment flavonoid with many useful biological properties. Quercetin is also a GRAS food additive approved by the US FDA. Adding quercetin to the packaging system improves the physical performance as well as the functional properties of the film. Therefore, this review focused on quercetin's effect on the various packaging film properties, such as mechanical, barrier, thermal, optical, antioxidant, antimicrobial, and so on. The properties of films containing quercetin depend on the type of polymer and the interaction between the polymer and quercetin. Films functionalized with quercetin are useful in extending shelf life and maintaining the quality of fresh foods. Quercetin-added packaging systems can be very promising for sustainable active packaging applications.

Chitosan/Starch-Based Active Packaging Film with N, P-Doped Carbon Dots for Meat Packaging.

Nitrogen, phosphorus-doped green-tea-derived carbon dots (NP-CDs) incorporated chitosan/starch (Chi/St) based multifunctional nanocomposite films were prepared. FE-SEM images verified a homogeneous distribution of CDs with minimum aggregation in the fabricated films. Incorporating NP-CDs led to enhanced UV-light blocking (93.1% of UV-A and ∼99.7% of UV-B) without significantly affecting the films' water transparency and water vapor permeability. Besides, incorporating NP-CDs into the Chi/St films enhanced antioxidant activity (98.0% for ABTS and 71.4% for DPPH) and displayed strong antibacterial activity against L. monocytogenes, E. coli, and S. aureus. Wrapping the meat in the prepared film and storing it at 20 °C has been shown to reduce bacterial growth (less than 2.5 Log CFU/g after 48 h) without significantly altering the actual color of the wrapped meat. The Chi/St film loaded with NP-CD has high potential as an active packaging material to ensure safety and extend the shelf life of meat products.

Alizarin: Prospects and sustainability for food safety and quality monitoring applications.

Active and intelligent food packaging has emerged to ensure food safety, quality, or spoilage monitoring and extend the shelf life of food. The development of intelligent packaging has accelerated significantly in recent years with a focus on monitoring changes in the quality of packaged products in real-time throughout the food supply chain. As one of the popular natural colorants, alizarin has attracted much consideration due to its excellent functional properties and quality to color change under varying pH. Alizarin is an efficient and cost-effective biomaterial with numerous biological features such as antioxidant, antibacterial, non-cytotoxic, and antitumor. This review focuses on an in-depth summary and prospects for alizarin as a natural and safe colorant that has the potential to be incorporated into intelligent packaging to track the freshness of packaged foodstuffs. The use of alizarin as an intelligent packaging agent shows huge potential for the application of food packaging and brings it one step closer to real-time monitoring of food quality throughout the supply chain. Finally, various limitations and future requirements are discussed to underscore the importance of developing alizarin-based intelligent functional food packaging systems.

Gelatin/poly(vinyl alcohol)-based functional films integrated with spent coffee ground-derived carbon dots and grapefruit seed extract for active packaging application.

Nanoparticles are attractive, functional additives with great potential to be applied in biomaterial and food packaging. However, these particles are not soluble in water, thus limiting their widespread application. Here, we report a facile fabrication of carbon dots (CDs) using the spent coffee ground as the carbon source through a hydrothermal method. The CD was added to the gelatin/poly(vinyl alcohol) (Gel/PVA) film and grapefruit seed extract (GSE) to prepare multifunctional packaging films. The functional films' physiochemical and functional properties and packaging application were investigated. The composite film showed good UV protection properties with a slight decrease in transparency. The composite film containing CD/GSE showed strong antioxidant activity, scavenging >38 % DPPH and 100 % ABTS radicals. The film also exhibited significant antibacterial activity against the foodborne pathogens Listeria monocytogenes and Escherichia coli, completely eradicating the growth of these bacteria within 9 h of exposure. The CD/GSE-incorporated Gel/PVA films were used for pork packaging. The films were able to enhance the pork shelf life by reducing the L. monocytogenes bacterial growth in meat by 2 Log CFU/mL lower than the control wrapping film. The multifunctional Gel/PVA films are expected to be used for the active packaging of meat products.

Cellulose nanofiber-based multifunctional films integrated with carbon dots and anthocyanins from Brassica oleracea for active and intelligent food packaging applications.

A new generation of carbon dot-based active and intelligent packaging films with UV blocking, antibacterial, and real-time sensing potentials was fabricated using Brassica oleracea (BO) extract. The cellulose nanofiber (CNF) was used to prepare the multifunctional intelligent nanocomposite film integrated with BO anthocyanins (BOA) and BO-biowaste-derived carbon dots (BO-CDs). The incorporation of 1.5 % BO-CD and 6 % BOA in the CNF matrix improved the physicochemical and UV blocking (>189 % increase) properties of the fabricated films. The synthesized BO-CD exhibits high fluorescence, UV absorption, antibacterial and antioxidant functions. It showed strong radical scavenging activity against ABTS (~90 %) and DPPH (~80 %) compared to the neat CNF film. Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) have shown enhanced compatibility and elemental composition of the BO-CDs/BOA additives in the CNF-polymer matrix. Packaging tests showed that the prepared film worked efficiently and non-destructively and was able to monitor the freshness of minced pork, fish, and shrimp in real-time through a distinct visual change from red to colorless/yellow during storage at 25 °C for 48 h. Active and intelligent films developed based on CNF/BO-CDs/BOA are expected to be applied as multifunctional packaging materials that can indicate quality changes and extend the shelf life of packaged perishable foods.

pH-Responsive strips integrated with resazurin and carbon dots for monitoring shrimp freshness.

Carbon dots (CDs) were synthesized via a one-step hydrothermal approach using tangerine peel (Tan) and resazurin (Res) to fabricate biocompatible indicators for food freshness. The CDs' pH-responsive mechanism, morphology, zeta potential, XPS, and optical and fluorescence analysis were investigated. The as-prepared tangerine peel/resazurin carbon dots (Tan/Res CDs) exhibited pH-responsive emission that changed from yellow to orange as the pH value increased. The Tan/Res CDs showed the sensing ability of ammonia with a detection limit of 0.84 µM by proportionally losing fluorescence intensity as the concentration increased from 1 to 100 µM. The CDs were coated onto paper strips to impart biogenic amine (BAs) detection for pH-responsive intelligent monitoring of packaged foods. The Tan/Res CDs paper-based indicator exhibited an impressive color change from yellow to brown during the detection of ammonia vapor. The indicator also showed the ability to detect BAs through a color change, demonstrating the ability to monitor the freshness of shrimp in situ. Additionally, the efficacy of the Tan/Res CDs indicator is validated by total volatile basic nitrogen (TVB-N), providing customers and suppliers with a simple, inexpensive, and portable tool to monitor the freshness of seafood in real-time.

Recent advances in metal sulfide nanoparticle-added bionanocomposite films for food packaging applications.

Metal sulfide nanoparticles have recently attracted much attention due to their unique physical and functional properties. Metal sulfide nanoparticles used as optoelectronic and biomedical materials in the past decades are promising for making functional nanocomposite films due to their low toxicity and strong antibacterial activity. Recently, copper sulfide and zinc sulfide nanomaterials have been used to produce food packaging films for active packaging. Metal sulfide nanoparticles added as nanofillers are attracting attention in packaging applications due to their excellent potential to improve mechanical, barrier properties, and antibacterial activity. This review covers the fabrication process and important applications of metal sulfide nanoparticles. The development of metal sulfides reinforcing mainly copper sulfide and zinc sulfide nanomaterials as multifunctional nanofillers in bio-based films for active packaging applications has been comprehensively reviewed. As the recognition of metal sulfide nanoparticles as a functional filler increases, the development and application potential of active packaging films using them is expected to increase.

Shikonin Functionalized Packaging Film for Monitoring the Freshness of Shrimp.

A shikonin embedded smart and active food packaging film was produced using a binary mixture of gelatin and cellulose nanofiber (CNF). Shikonin is an alcohol-soluble natural pigment extracted from Lithospermum erythrorhizon root. The fabricated film showed good pH-responsive color changes and volatile gas sensing properties. Moreover, the film exhibited excellent antioxidant and antibacterial activity against foodborne pathogens. The shikonin incorporated gelatin/CNF-based film showed excellent UV-light barrier properties (>95%) and high tensile strength (>80 MPa), which is useful for food packaging. The hydrodynamic properties of the film were also slightly changed in the presence of shikonin, but the thermal stability and water vapor permeability remained unaffected. Thus, the inclusion of shikonin in the gelatin/CNF-based film improves not only the physical properties but also the functional properties. The film's color indicator properties also clearly show shrimp's freshness and spoilage during storage for 48 h. The shikonin-based functional film is expected to be a promising tool for multi-purpose smart and active food packaging applications.

Pectin/carbon quantum dots fluorescent film with ultraviolet blocking property through light conversion.

Carbon quantum dots (CQDs) were synthesized using glucose as a carbon source through a hydrothermal method. CQDs showed negligible cytotoxicity to L929 cells even after prolonged exposure of 72 h. The addition of CQD did not affect the pectin film's mechanical properties, water contact angle, and thermal stability. However, the CQD-added composite film generates reactive oxygen species (ROS), providing high antibacterial activity against pathogenic bacteria (L. monocytogenes and E. coli) and antifungal activity against mold (Aspergillus flavus), where a 100% eradication of bacteria and fungi population was observed. Also, the addition of CQD strengthens the antioxidant activity of the composite films by 95%. Further, the CQD-added pectin film converted ultraviolet rays into blue light, which improved the film's UV protection properties. Therefore, the pectin/CQD film has a high potential for a light conversion active packaging film that may prevent the deterioration of high-fat foods.

Fabrication of Antioxidant and Antimicrobial Pullulan/Gelatin Films Integrated with Grape Seed Extract and Sulfur Nanoparticles.

Biopolymer-based functional blend films were prepared using pullulan and gelatin with functional fillers of sulfur nanoparticles (SNPs) and grape seed extract (GSE). A mixture of pullulan/gelatin (1:1) produced a compatible but slightly translucent free-standing film. SNPs capped with enoki mushroom extract and GSE were added as functional fillers to improve the properties (physical and functional) of the pullulan/gelatin-based film. The addition of SNP and GSE significantly (p < 0.05) boosted the UV-light barrier, water vapor barrier, and oxygen barrier properties of the pullulan/gelatin films. The mechanical performance of the pullulan/gelatin-based films was slightly decreased (∼10%), whereas the addition of fillers did not significantly affect the hydrophobicity and thermal stability. The addition of SNP provided the antimicrobial function against foodborne pathogenic bacteria, L. monocytogenes and E. coli, while GSE provided a powerful antioxidant activity to the pullulan/gelatin-based film. Therefore, pullulan/gelatin-based composite films with better UV, water vapor, and oxygen barrier properties and enhanced antioxidant and antibacterial properties are expected to have high utility in active food packaging applications.

Curcumin and its uses in active and smart food packaging applications - a comprehensive review.

Active and intelligent food packaging is an innovative technology to prevent food contamination and ensure food quality and safety. Active packaging protects the food from microbial contamination, while smart or intelligent packaging enables monitoring the freshness of the food or quality change in real-time. Curcumin, one of the most well-known natural colorants, has received a lot of attention for its excellent functional properties and ability to change color with changes in pH. Curcumin, the golden component of turmeric, a spice widely used in food since ancient times, is a cost-effective and abundant biomaterial with various biological properties such as antioxidant, antibacterial, antiviral, antitumor, and anti-inflammatory. Recently, active packaging or intelligent packaging systems have been actively developed using the functional properties of curcumin. In this review, we briefly reviewed curcumin's basic biological functions and discussed comprehensive and recent progress in using curcumin in various polymer-based active and smart food packaging applications.

Preparation of a shikonin-based pH-sensitive color indicator for monitoring the freshness of fish and pork.

A novel intelligent pH-responsive color indicator was prepared by adsorbing a natural naphthoquinone pigment, shikonin, onto cellulose paper. FTIR results indicated that shikonin was crosslinked with the cellulose of the indicator paper. The addition of shikonin increased antioxidant activity, thermal stability, and water resistance properties of the paper. The indicator changed the color from red to dark blue, depending on the pH of buffer solutions. Also, the indicator showed high stability after 4 months of storage and maintained high sensitivity to pH changes. This indicator was used to monitor fish and pork freshness during storage at room temperature, and the results showed a high correlation between the color change of the indicator and the pH change of the sample. The shikonin-adsorbed indicator with stable and sensitive color change depending on pH can be used in the intelligent food packaging applications to monitor the quality of packaged food in real-time.

Preparation of on-package halochromic freshness/spoilage nanocellulose label for the visual shelf life estimation of meat.

In this work, grape anthocyanins (GA) were embedded in bacterial nanocellulose (BNC) by ex-situ method to fabricate an easy-to-use colorimetric label. The label revealed visible color responses to the pH buffers (2-11). According to the color parameter results [L*, a*, b*, and total color difference (TCD)], the label also presented appropriate color stability during the 60-day storage. During the application in minced beef, the label was bright red on the 1st day of storage at 4 °C. In accordance with the meat quality parameters [TVB-N, total mesophilic count, and sensory attributes], the label turned into purplish-red color on the 3rd and 5th days of storage (medium freshness meat) and turned into blue on the 7th day, representing the spoilage state. All the mentioned color changes could be distinguished by naked eyes. A strong Pearson's correlation coefficient was obtained between the TCD values and meat quality parameters, confirming the capability of the pH-sensing label to correctly distinguish the fresh meat from the spoiled meat.

CMC and CNF-based alizarin incorporated reversible pH-responsive color indicator films.

Smart color-changing indicator films were prepared using two different types of cellulose (CMC and CNF) and pH-sensitive dye, alizarin. pH-responsive color indicator films were produced by ionization and deprotonation of hydroxyl groups of alizarin phenolic compounds. The X-ray diffraction pattern of the color indicator film showed a new weak diffraction peak at 2θ = 13°, indicating the semi-crystalline character of alizarin. The indicator film showed UV-vis light screening properties and radical scavenging activity with enhanced thermal stability. The indicator film showed a distinct color change of alizarin from yellow to purple in the pH range of 2-12. In addition, the color indicator film showed stable and reversible color changes even after repeated changes in environmental pH. The pH-responsive color indicator films are likely to be used as an acid or base gas sensor due to the rapid response and reversible color change to the pH change in the packaging environment.

Development of an easy-to-use colorimetric pH label with starch and carrot anthocyanins for milk shelf life assessment.

An intelligent freshness indicator was developed by immobilizing anthocyanins of black carrot (ABC) within the starch matrix (total anthocyanins content of 10 mg/100 mL) to monitor freshness/spoilage of milk. The microstructural, spectral, swelling and solubility properties as well as color stability (as a function of time, temperature and light) of the indicator at different pHs were characterized. The incorporation of ABC did not change the swelling index and water solubility. The prepared label showed visible color changes as a function of pH and excellent color stability after one month storage at different conditions. The total color difference (TCD) value of the indicator corresponded to the pH, acidity, and microbial growth of the pasteurized milk. The Pearson correlation coefficient showed a high correlation between TCD and pH (R = -0.979), while a high and positive correlation between TCD and acidity as well as TMC (R = 0.983 and 0.968, respectively) was observed. The developed label can discriminate fresh milk form the milk entered into the initial (TCD: 7.8 after 24 h) and final (TCD: 34.8 after 48 h) steps of spoilage. The fabricated label opens a new perspective to use anthocyanins-incorporated biopolymers in the milk intelligent packaging as a simple and easy-to-use freshness indicator.