Perturbations in indices of oxidative stress, oxidative DNA damage and lung function in chronic exposure to wood dust in Southern Nigeria.

OBJECTIVES: Oxidative stress (OS) and oxidative DNA damage accruing from chronic exposure to wood dust have been implicated in the development of chronic lung conditions among woodworkers. Indices of OS, inflammation, oxidative DNA damage and lung function in relation to duration of exposure to wood dust were assessed in woodworkers to determine their possible utility as risk evaluation indices for chronic lung conditions. METHODS: Ninety participants comprising 30 active woodworkers, 30 passive woodworkers, and 30 controls were enrolled into this cross-sectional study. The total plasma peroxides, total antioxidant capacity (TAC), oxidative stress index (OSI), malondialdehyde (MDA), reduced glutathione, nitric oxide, high sensitivity C-reactive protein (hs-CRP), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and peak expiratory flow rate (PEFR) were determined in all participants. RESULTS: Woodworkers had lower PEFR, TAC, and higher malondialdehyde, OSI, hs-CRP, and 8-OHdG compared to controls (p < 0.05). Active woodworkers had higher malondialdehyde, 8-OHdG, and hs-CRP compared to passive woodworkers (p < 0.05). Increasing duration of exposure to wood dust is associated with higher malondialdehyde, hs-CRP, and 8-OHdG in active woodworkers (p < 0.05) and higher 8-OHdG and hs-CRP in passive woodworkers (p < 0.05). Negative correlation was observed between hs-CRP and TAC (r=-0.367, p = 0.048) in active workers. CONCLUSION: The association of exposure to wood dust with elevated indices of inflammation, OS, lipid peroxidation, oxidative DNA damage, and reduction in antioxidants and peak expiratory flow rate; and the concomitant increase in oxidative DNA damage and inflammation with increasing duration of exposure suggest that these indices may be useful in predicting woodworkers at risk of development of chronic lung conditions.

Male Sexual Dysfunction, Leptin, Pituitary and Gonadal Hormones in Nigerian Males with Metabolic Syndrome and Type 2 Diabetes Mellitus.

BACKGROUND: Pituitary and gonadal dysfunctions resulting from increased adiposity leading to disturbances of sexual and reproductive functions have been reported in males with metabolic syndrome (MS) and type 2 diabetes mellitus (DM2). The aim of this study was to evaluate sexual dysfunction, leptin, and reproductive hormones in Nigerian males with MS and DM2. METHODS: Participants were 104 men (34 males with DM2, 17 men with MS and 53 men with normal body mass index (18.5-24.9 Kg/m (2)) without MS (controls)). The International Diabetes Federation (2005) criteria were used for MS diagnosis. Reproductive history, anthropometry, blood pressure (BP) and 10 ml fasting blood samples were obtained by standard methods. Fasting plasma glucose, total cholesterol, triglycerides and high density lipoprotein cholesterol were determined by enzymatic methods while low density lipoprotein cholesterol was calculated. Leptin, follicle stimulating hormone (FSH), luteinising hormone (LH), prolactin, testosterone and oestrogen were determined by enzyme immunoassay (leptin by Diagnostic Automation, Inc.; others by Immunometrics (UK) Ltd.) while oestrogen-testosterone ratio was calculated. Data analyzed using ANOVA, Chi square and multiple regression were statistically significant at p<0.05. RESULTS: Testosterone was significantly lower in MS than controls while oestradiol and ETR were significantly higher in MS compared with controls and DM2 group (p<0.05). ETR significantly predicted testosterone in all groups (p<0.05). Significantly lower libido was observed in men in MS than controls and DM2 groups (p<0.05). CONCLUSION: Sexual and reproductive dysfunction may be related to increased conversion of testosterone to oestrogen in increased adipose mass in men with metabolic syndrome and type 2 diabetes mellitus.