RESUMEN
The anticancer properties of well-defined molecules serve to bolster the field of metals in medicine. Such compounds, particularly those of platinum and their closely related structural analogs, continue to be potentially highly interesting to researchers and clinicians alike. The four octahedral organoplatinum(IV) compounds [Pt(CH3)2X2{bipy-R 2 }] (X = Br, I; bipy-R 2 = 2,2'-bipyridine, 2,2'-bipyridine-4,4'-dicarboxylic acid) have been isolated and structurally characterized by single-crystal X-ray diffraction. Nuclear magnetic resonance and infrared spectroscopic data are also tabulated as useful reference values. The anticancer potential of each compound was assessed via in vitro MTT assays, using human breast cancer cells (cell line ZR-75-1). EC50 values were determined as 11.5 µM for Pt(CH3)2Br2{bipy}; 3020 µM, for Pt(CH3)2Br2{bipy-(CO 2 H) 2 }; 6.1 µM, for Pt(CH3)2I2{bipy}; and 86.0 µM, for Pt(CH3)2I2{bipy-(CO 2 H) 2 }; for comparison, the EC50 value for cisplatin against the ZR-75-1 cells was 16.4 µM. The most cytotoxic of the four compounds Pt(CH3)2I2{bipy} undergoes reaction with glutathione in a THF/water mixture at 68°C very slowly.
RESUMEN
Recent advances in molecular biology and tissue engineering [9] have provided useful tools for improving surgery in general and plastic surgery in particular [11]. Advancing knowledge of cell function and easier ways to obtain active substances or even cells with specific activity have led to more frequent use of many surgical procedures. The active substances that participate in the restoration of damaged tissues are known as growth factors (fundamentally contained in platelets), cytokines, chemokines [16], and similar factors such as interleukins and tissue necrosis factors. Platelets, monocytes, and lymphocytes (T helper cells) as well as neutrophils and astrocytes are involved in the process. As potential cells, stem cells [15,23] also can be used, if obtained from the same patient. The authors used cells with well-known functions, which when added to the surgical procedures, seemed to promote the healing process and the restoration of damaged tissues. The simplest, most effective, and least expensive techniques were selected from trials and feasibility studies for wider use in all types of surgical procedures.