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1.
Plant Cell Environ ; 40(4): 462-472, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26754426

RESUMEN

Abiotic stress is one of the main threats affecting crop growth and production. An understanding of the molecular mechanisms that underpin plant responses against environmental insults will be crucial to help guide the rational design of crop plants to counter these challenges. A key feature during abiotic stress is the production of nitric oxide (NO), an important concentration dependent, redox-related signalling molecule. NO can directly or indirectly interact with a wide range of targets leading to the modulation of protein function and the reprogramming of gene expression. The transfer of NO bioactivity can occur through a variety of potential mechanisms but chief among these is S-nitrosylation, a prototypic, redox-based, post-translational modification. However, little is known about this pivotal molecular amendment in the regulation of abiotic stress signalling. Here, we describe the emerging knowledge concerning the function of NO and S-nitrosylation during plant responses to abiotic stress.


Asunto(s)
Óxido Nítrico/metabolismo , Plantas/metabolismo , Estrés Fisiológico , Modelos Biológicos , Óxido Nítrico/biosíntesis , Procesamiento Proteico-Postraduccional , Temperatura
2.
Interdiscip Sci ; 4(4): 256-67, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23354814

RESUMEN

Vps mediated vesicular transport is important for transferring macromolecules trapped inside a vesicle. Although highly abundant, Vps shows tremendous sequence variation among diverse array of species. However, this difference in sequence, which seems to also translate into substantial functional variation, is hardly characterized in Corchorus spp. Here, our computational study investigates structural and functional features of one of the Vps subunit namely Vps51/Vps67 in C. olitorius. Broad scale structural characterization revealed novel information about the overall Vps structure and binding sites. Moreover, functional analyses indicate interaction partners which were unexplored to date. Since membrane trafficking is essentially associated with nutrient uptake and chemical de-toxification, characterization of the Vps subunit can well provide us with better insight into important agronomic traits such as stress response, immune response and phytoremediation capacity.


Asunto(s)
Secuencia de Aminoácidos , Arabidopsis/química , Corchorus/química , Proteínas de Plantas/química , Subunidades de Proteína/química , Homología Estructural de Proteína , Proteínas de Transporte Vesicular/química , Arabidopsis/metabolismo , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/metabolismo , Sitios de Unión , Transporte Biológico , Biología Computacional/métodos , Corchorus/metabolismo , Proteínas de Plantas/metabolismo , Estructura Terciaria de Proteína , Subunidades de Proteína/metabolismo , Análisis de Secuencia/métodos , Relación Estructura-Actividad , Proteínas de Transporte Vesicular/metabolismo
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