RESUMEN
The leaf crown borer Eupalamides cyparissias (Cramer, 1775) is an important pest of coconut (Cocos nucifera L.) and other palms (Arecaceae) of economic importance, attacking the base of leaves, inflorescences, and infructescences, increasing fruit abortion. The objective of this study was to evaluate the spatial correlation of the infestation rate of E. cyparissias in coconut plantation blocks in the Brazilian Amazon, from January to December 2019, in the city of Santa Izabel, Pará, Brazil. The study area is a farm subdivided into 157 blocks of a commercial plantation of the green dwarf coconut. The Local Moran's Index was used to evaluate the existence of spatial autocorrelation of the E. cyparissias infestation rate in the 157 blocks with neighboring blocks. The infestation rate was calculated by the ratio between the number of plants attacked by the borer and the total number of plants in a block. There is a significant correlation of the symptomatology of the attack by E. cyparissias in the blocks of the experimental area, which indicates an aggregated pattern of distribution. There is no significant correlation between the attack by the borer and the age of the coconut tree; however there is a significant correlation between the attack by the borer and proximity to forest areas. These results indicate that forest regions are foci of infestation by the borer in coconut plantations.
Asunto(s)
Arecaceae , Lepidópteros , Animales , Cocos , Hojas de la Planta , BrasilRESUMEN
In semiarid agricultural regions, aquifers have watered widespread economic development. Falling water tables, however, drive up energy costs and can make the water toxic for human consumption. The study area is located in central Mexico, where arsenic and fluoride are widely present at toxic concentrations in well water. We simulated the holistic outcomes from three pumping scenarios over 100 years (2020-2120); (S1) pumping rates increase at a similar rate to the past 40 years, (S2) remain constant, or (S3) decrease. Under scenario S1, by 2120, the depth to water table increased to 426 m and energy consumption for irrigation increased to 4 × 109 kWh/yr. Arsenic and fluoride concentrations increased from 14 to 46 µg/L and 1.0 to 3.6 mg/L, respectively. The combined estimated IQ point decrements from drinking untreated well water lowered expected incomes in 2120 by 27% compared to what they would be with negligible exposure levels. We calculated the 100-year Net Present Value (NPV) of each scenario assuming the 2020 average crop value to water footprint ratio of 0.12 USD/m3. Without drinking water mitigation, S1 and S3 yielded relative NPVs of -5.96 × 109 and 1.51 × 109 USD, respectively, compared to the base case (S2). The relative NPV of providing blanket reverse osmosis treatment, while keeping pumping constant (S2), was 11.55 × 109 USD and this gain increased when combined with decreased pumping (S3). If a high value, low water footprint crop was substituted (broccoli, 1.51 USD/m3), the net gains from increasing pumping were similar in size to those of implementing blanket drinking water treatment.
RESUMEN
The objective of work was to study the productive profile of perennial peanut in a rotational stocking environment under different criteria. The treatments corresponded to pre-grazing height of 14 and 18cm or 95% of light interception (LI) distributed in a completely randomized design. The studied variables were forage mass, percentage of structural components, forage mass and the structural components in the lower and upper canopy strata. The entrance criterion of 18cm in height, despite having higher forage mass, presented lower percentage of leaves and higher percentage of stems and forage losses. The criteria of 14cm and 95% light interception presented similar production between them and the highest rate of forage accumulation. In all criteria, in the upper strata of pasture a higher percentage of leaves were found. The criteria of 95% LI and 14cm in height presented the best productive performances. The entry criterion of 18cm presented a higher mass of pre-grazing forage with lower percentage of dead material, but with higher forage losses, resulting from senescent leaves. Due to changes in the structural components, as grazing cycles increase, the interception of 95% of the incident light by the perennial peanut occurs at lower heights.(AU)
O objetivo do presente trabalho foi estudar o perfil produtivo do amendoim forrageiro em um ambiente rotacionado, sob diferentes critérios. Os tratamentos corresponderam à altura de pré-pastejo de 14 e 18cm ou 95% de intercepção de luz (LI), distribuídos em delineamento inteiramente ao acaso. As variáveis estudadas foram massa forrageira, porcentagem de componentes estruturais, densidade da massa forrageira e densidade dos componentes estruturais nos estratos do dossel inferior e superior. O critério de entrada de 18cm de altura, apesar de ter maior massa forrageira, apresentou menor porcentagem de folhas e maior porcentagem de hastes e perdas de forragem. Os critérios de interceptação de luz de 14cm e 95% apresentaram produção similar entre eles e a maior taxa de acumulação de forragem. Em todos os critérios, no estrato superior de pastagem, foi encontrada maior porcentagem de folhas. Os critérios de 95% LI e 14cm de altura apresentaram os melhores desempenhos produtivos. O critério de entrada de 18cm apresentou maior massa de forragem pré-pastagem com menor porcentagem de material morto, mas com maiores perdas de forragem, resultantes de folhas senescentes. Devido às mudanças nos componentes estruturais, à medida que os ciclos de pastagem aumentam, a intercepção de 95% da luz incidente pelo amendoim forrageiro ocorre em alturas mais baixas.(AU)
Asunto(s)
Arachis/crecimiento & desarrollo , OvinosRESUMEN
Objetivo: la microcirugía laríngea es un procedimiento terapéutico en lesiones benignas de laringe, diagnóstico en pacientes con disfonías de evolución variable y en casos de sospecha de malignidad. El objetivo de este estudio es describir las características de los pacientes intervenidos por microcirugía laríngea y las lesiones observadas en un servicio de otorrinolaringología en Argentina. Metodología: estudio retrospectivo. Se realizó la revisión de protocolos quirúrgicos, historias clínicas, videoestroboscopias computarizadas y documentos de anatomía patológica. Análisis univariado descriptivo con utilización de la t de Student o la Chi- cuadrado, según las características de las variables (p <0.005 para significancia estadística). Resultados: el sexo masculino fue el más intervenido, con una edad media de 51.2 ± 6.6 años. El antecedente de tabaquismo fue el más frecuente. Las principales lesiones macroscópicas fueron premalignas y malignas, pólipos y quistes. En la anatomía patológica se encontraron pólipos, carcinomas y quistes. En ambos sexos la lesión más frecuente fue el pólipo (33% vs. 45%, p =0.03). En pacientes con antecedente de tabaquismo la lesión más común fue pólipo, en ambos géneros, en menores de 60 años (46% vs. 30%, p =0.02), mientras en mayores de 60 años fue el carcinoma (33% vs. 15%, p <0.001). Conclusiones: en población masculina en sexta década de la vida se encontró principalmente presencia de pólipos, quistes y carcinomas. En pacientes de este género, con antecedente de tabaquismo, se describe mayor presencia de carcinomas.
Objective: Laryngeal microsurgery is a procedure indicated in benign laryngeal lesions; it is also a diagnostic tool in patients with dysphonia or in cases where malignancy is suspected. Methodology: Retrospective study in which clinical records, surgical protocols, videoestroboscopies, and pathology documents of patients under laryngeal microsurgery between 2011 and 2016 were reviewed. Univariate analysis was performed using Chi squared test (p <0.05). Results: Mean age was 51.2 ± 6.6 years, males were the most frequently intervened. Smoking was the most common comorbidity. The main macroscopic lesions observed were premalignant and malignant, polyps and cysts; in the pathology, polyps, carcinomas, and cysts were found. Polyps were the most frequent lesions found in both genders (33% vs 45%, p = 0.03). In patients with a smoking comorbidity, polyps were the most common lesion in both women and men (46% vs. 30%, p = 0.02). A greater presence of carcinomas was described in males over 60 with a history of smoking (33% vs 15%, p = 0.0001). Conclusions: Polyps, cysts, and carcinomas were mainly found in males over 60. A greater presence of carcinomas was described in male patients with a history of smoking.
Objetivo: a microcirurgia laríngea é um procedimento terapêutico em lesões benignas de laringe, diagnóstico em pacientes com disfonias de evolução variável e em casos de suspeita de malignidade. O objetivo deste estudo é descrever as características dos pacientes intervindos por microcirurgia laríngea e as lesões observadas num serviço de otorrinolaringologia na Argentina. Metodologia: estudo retrospectivo. Se realizou a revisão de protocolos cirúrgicos, histórias clínicas, videoestroboscopias computorizadas e documentos de anatomia patológica. Análise univariado descritivo com utilização da t de Student ou a Chi- quadrado, segundo as características das variáveis (p <0.005 para significância estatística). Resultados: o sexo masculino foi o mais intervindo, com uma idade média de 51.2 ± 6.6 anos. O antecedente de tabaquismo foi o mais frequente. As principais lesões macroscópicas foram pré-malignas e malignas, pólipos e quistos. Na anatomia patológica se encontraram pólipos, carcinomas e quistos. Em ambos sexos a lesão mais frequente foi o pólipo (33% vs. 45%, p =0.03). Em pacientes com antecedente de tabaquismo a lesão mais comum foi pólipo, em ambos gêneros, em menores de 60 anos (46% vs. 30%, p =0.02), enquanto em maiores de 60 anos foi o carcinoma (33% vs. 15%, p <0.001). Conclusões: na população masculina na sexta década da vida se encontrou principalmente presença de pólipos, quistos e carcinomas. Em pacientes deste gênero, com antecedente de tabaquismo, se descreve maior presença de carcinomas.
Asunto(s)
Humanos , Laringe , Otolaringología , Pólipos , Tabaquismo , Carcinoma , Fumar , Quistes , Disfonía , MicrocirugiaRESUMEN
Although some studies in sheep have indicated leptospire colonization of the genital tract, further studies are needed to clarify the role of genital carriers in this species. Thus, this study aimed to evaluate the colonization of pathogenic leptospires in the genital and urinary tract of slaughtered sheep. Fifty-seven adult, female woolless sheep destined for slaughter were used. Renal (n = 57), bladder (n = 57), ovary (n = 34), uterine tube (n = 44), and uterus (n = 33) samples were collected for molecular detection of Leptospira sp. DNA, and blood samples (n = 57) for serological testing. The molecular testing was performed using polymerase chain reaction (PCR), and the serological testing was performed using microscopic serum agglutination test (MAT). Samples with amplifying DNA were subjected to genetic sequencing. In total, leptospiral DNA was found in the tissues of 44 (77.2%) sheep, whereas only nine animals were positive on both PCR and MAT; there was slight agreement between PCR and MAT techniques (k = 0.0268; p = 0.684). In 61 (54.9%) genital tract and in five (4.4%) urinary tract samples, the leptospiral DNA was detected, with significant difference (p < 0.001). The genes of one sample from the uterine tube and another from the bladder were sequenced and demonstrated 99% similarity to Leptospira interrogans. Anti-Leptospira antibodies were detected in 11 (19.3%) of the tested animals. The results reinforce the importance of the genital tract as an extra-renal site of colonization, suggesting the possibility of venereal transmission in sheep.
Asunto(s)
Genitales/microbiología , Leptospira interrogans/aislamiento & purificación , Leptospirosis/veterinaria , Ovinos/microbiología , Sistema Urinario/microbiología , Pruebas de Aglutinación , Animales , Brasil/epidemiología , Femenino , Riñón/microbiología , Leptospira/inmunología , Leptospira interrogans/genética , Leptospirosis/epidemiología , Reacción en Cadena de la Polimerasa/veterinaria , Ovinos/genética , Útero/microbiologíaRESUMEN
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell cycle phases, and caspase-3 activity. Fetal weight was reduced in the diabetic group. Expression of the cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90, CD44, STRO-1, OCT3/4, and Nanog was detected in VYS cells in both groups. In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed. VYS cells with inactive mitochondria, activated caspase-3, and low proliferation were present in the rats with diabetes. Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.
Asunto(s)
Diabetes Mellitus Experimental/fisiopatología , Embarazo en Diabéticas/fisiopatología , Saco Vitelino/fisiopatología , Animales , Antígenos CD/metabolismo , Biomarcadores/metabolismo , Ciclo Celular/fisiología , Proliferación Celular , Supervivencia Celular , Femenino , Peso Fetal , Masculino , Embarazo , Ratas , Ratas WistarRESUMEN
Quantum dots exhibit extraordinary optical and mechanical properties, and the number of their applications is increasing. In order to investigate a possible effect of coating on the inhalation toxicity of previously tested non-coated CdS/Cd(OH)2 quantum dots and translocation of these very small particles from the lungs, rats were exposed to coated quantum dots or CdCl2 aerosol (since Cd(2+) was present as impurity), 6h/d for 5 consecutive days. Cd content was determined in organs and excreta after the end of exposure and three weeks thereafter. Toxicity was determined by examination of broncho-alveolar lavage fluid and microscopic evaluation of the entire respiratory tract. There was no evidence for translocation of particles from the respiratory tract. Evidence of a minimal inflammatory process was observed by examination of broncho-alveolar lavage fluid. Microscopically, minimal to mild epithelial alteration was seen in the larynx. The effects observed with coated quantum dots, non-coated quantum dots and CdCl2 were comparable, indicating that quantum dots elicited no significant effects beyond the toxicity of the Cd(2+) ion itself. Compared to other compounds with larger particle size tested at similarly low concentrations, quantum dots caused much less pronounced toxicological effects. Therefore, the present data show that small particle sizes with corresponding high surfaces are not the only factor triggering the toxic response or translocation.
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Compuestos de Cadmio/toxicidad , Glutaral/toxicidad , Hidróxidos/toxicidad , Puntos Cuánticos/toxicidad , Sistema Respiratorio/efectos de los fármacos , Sulfuros/toxicidad , Aerosoles , Animales , Líquido del Lavado Bronquioalveolar , Cloruro de Cadmio/toxicidad , Compuestos de Cadmio/metabolismo , Compuestos de Cadmio/orina , Heces/química , Glutaral/metabolismo , Glutaral/orina , Hidróxidos/metabolismo , Hidróxidos/orina , Exposición por Inhalación , Masculino , Tamaño de la Partícula , Puntos Cuánticos/metabolismo , Ratas , Ratas Wistar , Sistema Respiratorio/metabolismo , Sistema Respiratorio/patología , Sulfuros/metabolismo , Sulfuros/orina , Factores de Tiempo , Distribución Tisular , Pruebas de Toxicidad AgudaRESUMEN
Colloidal quantum dots (QD) show great promise as fluorescent markers. The QD used in this study were obtained in aqueous medium rather than the widely used colloidal QD. Both methodologies used for the production of QD are associated with the presence of heavy metals such as cadmium (Cd). Here we investigate the short-term inhalation toxicity of water-soluble core-shell CdS/Cd(OH)2 QD. Male Wistar rats were head-nose exposed for 6 h/day on 5 days at the technically maximum concentration (0.52 mg Cd/m³). Histological examination was performed directly after the last exposure. Additional rats were used for Cd organ burden determinations. Clinical parameters in blood, bronchoalveolar lavage fluid and lung tissue were determined 3 days after the last exposure. To analyze the reversibility or progression of effects, the examinations were performed again after a recovery period of 3 weeks. The results of the study indicate that CdS/Cd(OH)2 QD caused local neutrophil inflammation in the lungs that partially regressed after the 3-week recovery period. There was no evidence that QD were translocated to the central nervous system nor that a systemic acute phase response occurred.
Asunto(s)
Compuestos de Cadmio/toxicidad , Pulmón/efectos de los fármacos , Sulfuros/toxicidad , Aerosoles , Animales , Compuestos de Cadmio/administración & dosificación , Inflamación/inducido químicamente , Exposición por Inhalación , Masculino , Microscopía Electrónica de Transmisión , Neutrófilos/efectos de los fármacos , Puntos Cuánticos , Ratas , Ratas Wistar , Sulfuros/administración & dosificación , Distribución TisularRESUMEN
A hemácia é carregada negativamente, principalmente devido ao ácido siálico que gera um potencial elétrico denominado Potencial Zeta que impede a aglutinação intravascular. Os testes de hemaglutinação na rotina transfusional, necessitam de substâncias potencializadoras, das quais muitas agem diminuindo o Potencial Zeta para se ter maior sensibilidade. Através da pinça óptica, ferramenta capaz de capturar células utilizando a luz, foi proposta uma metodologia para quantificar o potencial zeta e aplicar em hemácias coletadas com EDTA e estocadas em CPD-SAGM (visando avaliar alterações de cargas da membrana relacionadas a lesões de armazenamento. Os potenciais zeta em CPD-CAGM foram superiores (-14,8 mV) aos em EDTA (-7,9 mV) e decrescentes a partir do primeiro dia de armazenamento, estabilizando-se a partir da terceira semana com potencial zeta -7,6 mV. Hemácias com CPD-SAGM apresentaram potencial zeta maior, pois possivelmente este conservante evitou lesões mais significativas da membrana que poderiam alterar as cargas. A redução do potencial zeta no armazenamento pode ser consequência de enzimas liberadas de leucócitos lisados que tenham alterado as glicoforinas da membrana. A metodologia permitiu avaliar o potencial zeta em diferentes condições e poderá contribuir na padronização de técnicas de hemaglutinação com diferentes meios potencializadores e no melhor conhecimento das lesões de estocagem para fins transfusionais.
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Ácido Edético , Transfusión de Eritrocitos , Eritrocitos , Pruebas de Hemaglutinación , Hematología , Pinzas Ópticas , Potencial zetaRESUMEN
BACKGROUND: There is mounting evidence that deteriorated semen quality may be associated with increased serum concentration of 1,1,1-trichloro-2,2-bis(chlorodiphenyl)ethane (DDT) and its metabolites. The problem is exacerbated in situations where DDT is the only resource available to control malaria mosquitoes and DDT metabolite plasma concentration can reach 1000-fold the level found in other populations. There are limited and contradictory epidemiological data on whether DDT/dichlorodiphenyl-dichloroethylene (DDE) can also damage sperm DNA. Therefore, there is a need to investigate the possible adverse effects on human sperm genetic integrity in a sufficiently large study population with adequate exposure contrasts, especially in the high exposure range. METHODS: We conducted a cross-sectional study, recruiting 209 young males from three communities in an endemic malaria area where DDT is sprayed annually. Blood plasma p,p'-DDT and its metabolite p,p'-DDE levels were measured and expressed as lipid adjusted p,p'-DDT and p,p'-DDE values. The sperm chromatin structure assay and Aniline Blue test were used to assess sperm DNA/chromatin integrity. RESULTS: The lipid adjusted p,p'-DDT mean (+/-SD) and median concentrations were 109.2 (+/-106.6) and 83.9 microg/g, respectively; and the lipid adjusted p,p'-DDE mean (+/-SD) and median concentrations were 246.2 (+/-218.5) and 177.8 microg/g, respectively. The results point to a weak association between DDT/DDE plasma concentration and the incidence of sperm with chromatin defects. CONCLUSIONS: The results suggest that non-occupational environmental DDT exposure may have a negative impact on sperm chromatin integrity in young South African males.
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Cromatina/efectos de los fármacos , DDT/toxicidad , Espermatozoides/efectos de los fármacos , Adolescente , Adulto , Estudios Transversales , DDT/sangre , Daño del ADN , Fragmentación del ADN , Diclorodifenil Dicloroetileno/sangre , Citometría de Flujo , Humanos , Masculino , Análisis de Semen , SudáfricaRESUMEN
Beads trapped by an optical tweezers can be used as a force transducer for measuring forces of the same order of magnitude as typical forces induced by flagellar motion. We used an optical tweezers to study chemotaxis by observing the force response of a flagellated microorganism when placed in a gradient of attractive chemical substances. This report shows such observations for Leishmania amazonensis, responsible for leishmaniasis, a serious disease. We quantified the movement of this protozoan for different gradients of glucose. We were able to observe both the strength and the directionality of the force. The characterization of the chemotaxis of these parasites can help to understand the mechanics of infection and improve the treatments employed for this disease. This methodology can be used to quantitatively study the taxis of any kind of flagellated microorganisms under concentration gradients of different chemical substances, or even other types of variable gradients such as temperature and pressure.
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Quimiotaxis , Leishmania mexicana/fisiología , Locomoción , Microscopía por Video/métodos , Pinzas Ópticas , Animales , Factores Quimiotácticos/farmacología , Glucosa/farmacologíaRESUMEN
The main of this work is to determine the elemental and absorption distribution map in breast tissue samples by X-ray fluorescence and X-ray transmission microtomography. The experiments were performed at the X-ray fluorescence beamline (D09B-XRF) in the Brazilian Synchrotron Light Source (LNLS), Campinas, Brazil. A quasi-monochromatic beam produced by a multilayer monochromator was used as an incident beam. The sample was placed on a high precision goniometer and translation stages that allow rotating as well as translating it perpendicularly to the beam. The fluorescence photons were collected with an energy dispersive HPGe detector placed at 90 degrees to the incident beam, while transmitted photons were detected with a fast Na(Tl) scintillation counter () placed behind the sample on the beam direction. All the tomographic images were reconstructed using a filtered-back projection algorithm. In the breast tissue samples, it was possible to determine the distribution of Zn, Cu and Fe in healthy tissue and tumor.
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Neoplasias de la Mama/diagnóstico por imagen , Neoplasias de la Mama/metabolismo , Mama/metabolismo , Mamografía/métodos , Metales/análisis , Refractometría/métodos , Tomografía por Rayos X/métodos , Algoritmos , Humanos , Imagenología Tridimensional/métodos , Intensificación de Imagen Radiográfica/métodos , Interpretación de Imagen Radiográfica Asistida por Computador/métodos , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Distribución TisularRESUMEN
Damos a conocer un caso de gestación cuádruple espontánea, el manejo y su resolución.
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Masculino , Femenino , Embarazo , Recién Nacido , Adulto , Humanos , Complicaciones del Embarazo/terapia , Embarazo Múltiple , Cuádruples , Gemelos , Puntaje de Apgar , Cesárea , Resultado del EmbarazoRESUMEN
Fetal malignancies that are capable of metastasizing to other fetal organs and the placenta are exceedingly rare. Fetal tumors are suspected on ultrasonography in the presence of structures of abnormal shape or size that are occasionally associated with polyhydramnios and hydrops. Most of the large abdominal masses detected antenatally are renal in origin, although adrenal tumors should also be kept in mind in their differential diagnosis. We report a case of a large-for-gestational-age fetus with abdominal distention secondary to bilateral adrenal carcinoma, polyhydramnios and placental enlargement. Postmortem histological findings included nesidioblastosis, (i.e. hyperplasia of the cells of the islets of Langerhans) and adrenocortical cytomegaly, suggestive of a form of Beckwith-Wiedemann syndrome.
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Neoplasias de las Glándulas Suprarrenales/patología , Síndrome de Beckwith-Wiedemann/patología , Enfermedades Fetales/patología , Aborto Espontáneo , Adenoma de Células de los Islotes Pancreáticos/patología , Neoplasias de las Glándulas Suprarrenales/diagnóstico por imagen , Adulto , Diagnóstico Diferencial , Resultado Fatal , Femenino , Enfermedades Fetales/diagnóstico por imagen , Edad Gestacional , Humanos , Recién Nacido , Metástasis de la Neoplasia/patología , Neoplasias Pancreáticas/patología , Embarazo , Ultrasonografía Doppler en Color , Ultrasonografía Prenatal/métodosRESUMEN
We report a new and efficient methodology of labelling red blood cells, in order to investigate the expression of anti-A antigen, employing luminescent semiconductor nanocrystals. Highly luminescent and stable core-shell cadmium sulphide/cadmium hydroxide [CdS/CdS(OH)2] colloidal particles were obtained in the nanometre size range. The surface of these particles was characterized by using a monoclonal anti-A antibody via a one-step glutaraldehyde cross-linking procedure, followed by conjugation of the particles to red cells of blood groups A+, and O+. Laser scanning confocal microscopy images indicated that after conjugation for 30 min, A+ and erythrocytes presented different patterns of dual bright emission whereas the O+ group cells showed no emission. We suggest that this labelling procedure may be applied as a quantitative tool to investigate the distribution and expression of alloantigen in red blood cells.
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Compuestos Cromogénicos/síntesis química , Membrana Eritrocítica/metabolismo , Nanotecnología/métodos , Puntos Cuánticos , Aleaciones , Anticuerpos Monoclonales/inmunología , Complejo Antígeno-Anticuerpo/análisis , Cadmio/química , Compuestos Cromogénicos/metabolismo , Humanos , Ensayo de MaterialesRESUMEN
The time of food availability induces important behavioral and metabolic adaptations. Animals subjected to feeding restricted to a few daytime hours show increased locomotor activity and body temperature in anticipation of mealtime. In addition, animals under ad libitum feeding show a marked postprandial raise in body temperature and in thermogenesis. The areas of the brain commanding these responses to food are partially known. We investigated in the rat the role of the infralimbic area, located in the medial prefrontal cortex, and considered a visceral-autonomic motor area, in the responses to ad libitum or restricted feeding schedule. We performed infralimbic cortex excitotoxic lesions using injections of ibotenic acid, and measured body temperature and locomotor activity by telemetry in rats under ad libitum and restricted feeding conditions. We found that bilateral infralimbic area lesions prevented both the anticipatory and the postprandial increases in core temperature, decreased mean temperature by nearly 0.3 degrees C during both light/dark phases, and increased daily temperature variability. In contrast, the lesion caused a rapid induction of the anticipatory locomotor activity. These results show that behavioral and metabolic responses to the time of food availability are commanded separately and that the infralimbic area is a key structure to adjust the body temperature to an upcoming meal.
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Temperatura Corporal/fisiología , Conducta Alimentaria/fisiología , Sistema Límbico/fisiología , Actividad Motora/fisiología , Animales , Ritmo Circadiano , Agonistas de Aminoácidos Excitadores/farmacología , Ácido Iboténico/farmacología , Sistema Límbico/lesiones , Masculino , Ratas , Ratas Sprague-Dawley , Factores de TiempoRESUMEN
Arousal depends on the concerted activity of the ascending arousal system (AAS) but specific stimuli may primarily activate some nuclei of this system. Motivated behaviours are characterized by behavioural arousal, although it is not known which AAS nuclei are active during a motivated behaviour. To address this issue, rats were rendered motivated for food by fasting them for 1 day and then were enticed with food that they could not obtain for varying periods of time. We studied the level of arousal by polysomnography or radiotelemetry, and Fos-ir in the AAS, during food enticing. We found a strong arousal and an early increase in Fos-ir in the histaminergic neurons from the tuberomammillary nucleus, after 30 min of enticing, followed by increased Fos-ir in the whole AAS if food enticing was prolonged to 1 or 2 hours. In contrast, food presentation to non-motivated rats did not increase arousal or Fos-ir in the tuberomammillary nucleus. As opposed to the active arousal of the motivated rats, passive arousal induced by sensory stimulation was associated with increased Fos-ir in the locus coeruleus and the orexin neurons, but not with increased Fos-ir in the tuberomammillary nucleus or in the other nuclei of the AAS. We conclude that the arousal during feeding-related motivated behaviour is associated primarily with the activation of the tuberomammillary nucleus, while the other arousal-related nuclei become active later on.
Asunto(s)
Nivel de Alerta/fisiología , Conducta Alimentaria/fisiología , Regulación de la Expresión Génica/fisiología , Histamina/metabolismo , Motivación , Neuronas/metabolismo , Proteínas Proto-Oncogénicas c-fos/metabolismo , Vías Aferentes/citología , Vías Aferentes/metabolismo , Animales , Conducta Animal , Temperatura Corporal/fisiología , Recuento de Células/métodos , Alimentos , Hipotálamo/citología , Hipotálamo/metabolismo , Inmunohistoquímica/métodos , Masculino , Actividad Motora/fisiología , Ratas , Ratas Sprague-Dawley , Factores de Tiempo , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
In this work, we studied the effects of kaurenoic acid, a diterpene isolated from the oleo-resin of Copaifera langsdorffii in developing sea urchin (Lytechinus variegatus) embryos, on tumor cell growth in microculture tetrazolium (MTT) test and on mouse and human erythrocytes in hemolysis assay. Continuous exposure of embryos to kaurenoic acid starting immediately after fertilization inhibited the first cleavage (IC(50): 84.2 microM) and progressively induced embryo destruction (IC(50): 44.7 microM and < 10 microM for blastulae and larvae stages, respectively). In MTT assay, kaurenoic acid at a concentration of 78 microM produced growth inhibition of CEM leukemic cells by 95%, MCF-7 breast and HCT-8 colon cancer cells by 45% each. Further, kaurenoic acid induced a dose-dependent hemolysis of mouse and human erythrocytes with an EC(50) of 74.0 and 56.4 microM, respectively. The destruction of sea urchin embryos, the inhibition of tumor cell growth and the hemolysis of mouse and human erythrocytes indicate the potential cytotoxicity of kaurenoic acid.
Asunto(s)
Citotoxinas/toxicidad , Diterpenos/toxicidad , Plantas Medicinales/química , Resinas de Plantas/química , Erizos de Mar/fisiología , Teratógenos/toxicidad , Animales , Supervivencia Celular/efectos de los fármacos , Citotoxinas/química , Diterpenos/química , Embrión no Mamífero , Eritrocitos/efectos de los fármacos , Hemólisis/efectos de los fármacos , Humanos , Técnicas In Vitro , Espectroscopía de Resonancia Magnética , Ratones , Teratógenos/química , Células Tumorales CultivadasRESUMEN
This study shows that pentoxifylline (ptx), a xanthine derivative, significantly attenuates scopolamine-induced memory impairment in rats, as demonstrated in a passive avoidance task (50 mg/kg intraperitoneally [i.p.]) and in an elevated T-maze (10 and 50 mg/kg i.p.). Ptx (25, 50 and 100 mg/kg i.p.) also potentiates oxotremorine-induced tremors in mice, in a dose-dependent manner, and this effect was completely prevented by atropine. In addition, ptx (50 and 100 mg/kg i.p.) increased the number of animals developing pilocarpine-induced seizures, and potentiated the latency to the first pilocarpine-induced convulsion. Hippocampus homogenates from rats treated with ptx (100 mg/kg) for 1 week and sacrificed 15 min after the last injection showed a significant decrease in the muscarinic receptor numbers, indicative of a downregulation phenomenon. Similar effects were observed when assays were performed 24 h after the last ptx injection (10 and 50 mg/kg i.p.), but not after 72 h. Additionally, in vitro assays showed that ptx inhibits acetylcholinesterase activity in a dose-dependent manner when incubated with homogenates from rat hippocampus. Our data suggest that the muscarinic agonist effect of ptx could possibly depend on factors such as endogenous cholinergic activity.
Asunto(s)
Discapacidades para el Aprendizaje/inducido químicamente , Pentoxifilina/farmacología , Receptores Muscarínicos/efectos de los fármacos , Acetilcolinesterasa/metabolismo , Animales , Ansiedad/psicología , Reacción de Prevención/efectos de los fármacos , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Discapacidades para el Aprendizaje/psicología , Masculino , Trastornos de la Memoria/inducido químicamente , Trastornos de la Memoria/psicología , Ratones , Agonistas Muscarínicos/farmacología , Antagonistas Muscarínicos/toxicidad , Oxotremorina/farmacología , Pilocarpina/antagonistas & inhibidores , Pilocarpina/toxicidad , Ratas , Ratas Wistar , Escopolamina/antagonistas & inhibidores , Escopolamina/toxicidad , Convulsiones/inducido químicamente , Convulsiones/prevención & control , Temblor/inducido químicamente , Temblor/prevención & controlRESUMEN
In recent years, the productivity of cotton in Brazil has been progressively decreasing, often the result of the reniform nematode Rotylenchulus reniformis. This species can reduce crop productivity by up to 40%. Nematodes can be controlled by nematicides but, because of expense and toxicity, application of nematicides to large crop areas may be undesirable. In this work, a methodology using geostatistics for quantifying the risk of nematicide application to small crop areas is proposed. This risk, in economic terms, can be compared to nematicide cost to develop an optimal strategy for Precision Farming. Soil (300 cm(3)) was sampled in a regular network from a R. reniformis-infested area that was a cotton monoculture for 20 years. The number of nematodes in each sample was counted. The nematode number per volume of soil was characterized using geostatistics, and 100 conditional simulations were conducted. Based on the simulations, risk maps were plotted showing the areas where nematicide should be applied in a Precision Farming context. The methodology developed can be applied to farming in countries that are highly dependent on agriculture, with useful economic implications.