RESUMEN
This article critically reviews the current state of knowledge on the quality of animal-source foods according to animal production and food processing conditions, including consumer expectations-behaviours and the effects of consumption of animal-source foods on human health. Quality has been defined through seven core attributes: safety, commercial, sensory, nutritional, technological, convenience, and image. Image covers ethical, cultural and environmental dimensions associated with the origin of the food and the way it is produced and processed. This framework enabled to highlight the priorities given to the different quality attributes. It also helped to identify potential antagonisms and synergies among quality attributes, between production and processing stages, and among stakeholders. Primacy is essentially given to commercial quality attributes, especially for standard commodity animal-source foods. This primacy has strongly influenced genetic selection and farming practices in all livestock commodity chains and enabled substantial quantitative gains, although at the expense of other quality traits. Focal issues are the destructuration of chicken muscle that compromises sensory, nutritional and image quality attributes, and the fate of males in the egg and dairy sectors, which have heavily specialised their animals. Quality can be gained but can also be lost throughout the farm-to-fork continuum. Our review highlights critical factors and periods throughout animal production and food processing routes, such as on-farm practices, notably animal feeding, preslaughter and slaughter phases, food processing techniques, and food formulation. It also reveals on-farm and processing factors that create antagonisms among quality attributes, such as the castration of male pigs, the substitution of marine-source feed by plant-based feed in fish, and the use of sodium nitrite in meat processing. These antagonisms require scientific data to identify trade-offs among quality attributes and/or solutions to help overcome these tensions. However, there are also food products that value synergies between quality attributes and between production and processing phases, particularly Geographical Indications, such as for cheese and dry-cured ham. Human epidemiological studies have found associations between consumption of animal-source foods and increased or decreased risk for chronic non-communicable diseases. These associations have informed public health recommendations. However, they have not yet considered animal production and food processing conditions. A concerted and collaborative effort is needed from scientists working in animal science, food process engineering, consumer science, human nutrition and epidemiology in order to address this research gap. Avenues for research and main options for policy action are discussed.
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Alimentación Animal , Carne , Animales , Ganado , Masculino , Estado Nutricional , PorcinosRESUMEN
The hen's egg (Gallus gallus) is an animal product of great agronomic interest, with a world production of 70.9 million tonnes in 2018. China accounted for 35% of world production, followed by North America (12% of world production), the European Union (7.0 million tonnes, 10% of world production) and India (5.0 million tonnes, 7% of world production). In France, 16-17 billion eggs are produced annually (14.5 billion for table eggs) and more than 1 200 billion worldwide. In 2019, egg production increased by 3.3% compared to 2018, mainly due to the increase in Asian production, which has risen by 42% since 2000. Chicken eggs are widely used either as a low-cost, high nutritional quality food cooked by the consumer (more than 100 billion eggs consumed in Europe), or incorporated as an ingredient in many food products. The various production methods have changed considerably over the last 15 years with the consideration of animal welfare and changes in European regulations. In Europe, fewer and fewer eggs are produced in confinement and there has been a strong growth in the number of systems giving access to an outdoor run. In this review, we describe the different ways in which eggs are produced and processed into egg products to meet the growing demand for ready-to-use food products. We analyse the effect of this evolution of hen-rearing systems on the set of characteristics of eggs and egg products that determine their quality. We describe the risks and benefits associated with these new production methods and their influence or lack of influence on commercial, nutritional, microbial and chemical contamination risk characteristics, as well as the evolution of the image for the consumer. The latter covers the ethical, cultural and environmental dimensions associated with the way the egg is produced.
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Pollos , Óvulo , Bienestar del Animal , Animales , Huevos , Femenino , Calidad de los Alimentos , América del NorteRESUMEN
The 'organic' label guarantees a production process that avoids the use of synthetic fertilisers, pesticides and hormones and minimises the use of veterinary drugs; however, consumers are demanding guarantees regarding food quality. This article reviews the current state of knowledge on the quality of organic animal products, including the authentication of their organic origin. Quality has been considered as an integrative combination of six core attributes: commercial value, and nutritional, sensory, technological, convenience and safety attributes. The comparison of these attributes between organic and conventional animal products shows high heterogeneity due to variability in farming pratices in both organic and conventional systems. To overcome this, we pinpoint the farming practices underlying the differences observed. This enables light to be shed on the consequences of possible trajectories of organic farming, if specifications are relaxed or tightened up on commitments concerning farming practices that impact product quality. Two recent meta-analyses showed better nutritional attributes in organic milk and meat linked to their higher poly-unsaturated fatty acid (PUFA) content, particularly n-3 PUFAs. Regarding safety, we point to a lack of integrated studies quantifying the balance between positive and negative effects. Organic farming reduces the risk of drug residues and antibiotic resistance, but both outdoor rearing and a frequently longer rearing period increase the animals' exposition to environmental contaminants and the risk of their bioaccumulation in milk, eggs, meat and fish flesh. We highlight antagonisms between quality attributes for certain animal products (lamb, pork). In general, attributes are more variable for organic products, which can be explained by lower genetic selection (poultry), lower inputs and/or greater variability in farming conditions. However, the literature does not address the implications of this greater variability for the consumers' acceptability and the necessary adaptation of manufacturing processes. Further research is needed to document the impacts on human nutritional biomarkers and health. Methods used to authenticate organic origin are based on differences in animal diet composition between organic and conventional systems, but their reliability is hampered by the variability in farming practices.
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Agricultura Orgánica , Óvulo , Animales , Alimentos Orgánicos , Leche/química , Aves de Corral , Reproducibilidad de los Resultados , OvinosRESUMEN
The use of chlordecone (CLD), a chlorinated polycyclic pesticide used in the French West Indies banana fields between 1972 and 1993, resulted in a long-term pollution of agricultural areas. It has been observed that this persistent organic pollutant (POP) can transfer from contaminated soils to food chain. Indeed, CLD is considered almost fully absorbed after involuntary ingestion of contaminated soil by outdoor reared animals. The aim of this study was to model toxicokinetics (TKs) of CLD in growing pigs using both non-compartmental and nonlinear mixed-effects approaches (NLME). In this study, CLD dissolved in cremophor was intravenously administrated to 7 Creole growing pigs and 7 Large White growing pigs (1 mg kg-1 body weight). Blood samples were collected from time t = 0 to time t = 84 days. CLD concentrations in serum were measured by GCMS/MS. Data obtained were modeled using Monolix (2019R). Results demonstrated that a bicompartmental model best described CLD kinetics in serum. The influence of covariates (breed, initial weight and average daily gain) was simultaneously evaluated and showed that average daily gain is the main covariate explaining inter-individual TKs parameters variability. Body clearance was of 76.7 mL kg-1 d-1 and steady-state volume of distribution was of 6 L kg-1. This modeling approach constitutes the first application of NLME to study CLD TKs in farm animals and will be further used for rearing management practices in contaminated areas.
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Clordecona/toxicidad , Insecticidas/toxicidad , Animales , Clordecona/análisis , Ingestión de Alimentos , Contaminantes Ambientales , Insecticidas/análisis , Cinética , Modelos Químicos , Musa , Contaminantes del Suelo/análisis , Porcinos , Toxicocinética , Indias OccidentalesRESUMEN
Chlordecone (Kepone or CLD) is a highly persistent pesticide formerly used in French West Indies. Nowadays high levels of this pesticide are still found in soils which represent a subsequent source of contamination for outdoor-reared animals. In that context, sequestering matrices like biochars or activated carbons (ACs) are believed to efficiently decrease the bioavailability of such compounds when added to contaminated soils. The present study intends to test the respective efficiency of soil amendment strategies using commercial ACs or biochars (obtained by a 500⯰C or 700⯰C pyrolysis of 4 distinct type of wood). This study involved three experimental steps. The first one characterized specific surface areas of biochars and ACs. The second one assessed CLD-availability of contaminated artificial soils (50⯵gâ¯g-1 of Dry Matter) amended with 5% of biochar or AC (mass basis). The third one assessed CLD bioavailability of those artificial soils through an in vivo assay. To limit ethically the number of animals, selections of the most promising media were performed between each experimental steps. Forty four castrated male 40-day-old piglets were exposed during 10 day by amended artificial soils according to their group (nâ¯=â¯4). Only treatment groups exposed through amended soil with AC presented a significant decrease of concentrations of CLD in liver and adipose tissue in comparison with the control group (pâ¯<â¯0.001). A non-significant decrease was obtained by amending artificial soil with biochars. This decrease was particularly high for a coconut shell activated carbon were relative bioavailability was found lower than 3.2% for both tissues. This study leads to conclude that AC introduced in CLD contaminated soil should strongly reduce CLD bioavailability.
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Carbón Orgánico/química , Clordecona/farmacocinética , Animales , Disponibilidad Biológica , Clordecona/análisis , Clordecona/química , Insecticidas , Suelo/química , Contaminantes del Suelo/análisis , Contaminantes del Suelo/química , Contaminantes del Suelo/farmacocinética , Porcinos , Distribución Tisular , Indias OccidentalesRESUMEN
Soil is the main matrix which contributes to the transfer of environmental pollutants to animals and consequently into the food chain. In the French West Indies, chlordecone, a very persistent organochlorine pesticide, has been widely used on banana growing areas and this process has resulted in a long-term pollution of the corresponding soils. Domestic outside-reared herbivores are exposed to involuntary soil intake, and tethered grazing commonly used in West Indian systems can potentially favour their exposure to chlordecone. Thus, it appears necessary to quantify to what extent grazing conditions will influence soil intake. This experiment consisted of a cross-over design with two daily herbage allowance (DHA) grazed alternatively. Six young Creole bulls were distributed into two groups (G1 and G2) according to their BW. The animals were individually tethered and grazed on a restrictive (RES) or non-restrictive (NRES) levels of DHA during two successive 10-days periods. Each bull progressed on a new circular area every day. The two contrasting levels of DHA (P0.05) due to the lower DMI of RES compared with NRES treatment.
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Crianza de Animales Domésticos/métodos , Bovinos/crecimiento & desarrollo , Clordecona/análisis , Conducta Alimentaria/fisiología , Contaminantes del Suelo/análisis , Suelo/química , Animales , Estudios Cruzados , Insecticidas/análisis , Masculino , Clima Tropical , Indias OccidentalesRESUMEN
Deuterium oxide dilution space (DOS) determination is one of the most accurate methods for in vivo estimation of ruminant body composition. However, the time-consuming vacuum sublimation of blood preceding infrared spectroscopy analysis, which is traditionally used to determine deuterium oxide (DO) concentration, limits its current use. The use of isotope-ratio mass spectrometry (IRMS) to determine the deuterium enrichment and thus quantify DO in plasma could counteract this limitation by reducing the sample preparation for plasma deproteinisation through centrifugal filters. The aim of this study was to validate the DOS technique using IRMS in growing goat kids to establish in vivo prediction equations of body composition. Seventeen weaned male Alpine goat kids (8.6 wk old) received a hay-based diet supplemented with 2 types of concentrates providing medium ( = 9) or high ( = 8) energy levels. Kids were slaughtered at 14.0 ( = 1, medium-energy diet), 17.2 ( = 4, medium-energy diet, and = 4, high-energy diet), or 21.2 wk of age ( = 4, medium-energy diet, and = 4, high-energy diet). Two days before slaughter, DOS was determined after an intravenous injection of 0.2 g DO/kg body mass (BM) and the resulting study of DO dilution kinetics from 4 plasma samples (+5, +7, +29, and +31 h after injection). The deuterium enrichment was analyzed by IRMS. After slaughter, the gut contents were discarded, the empty body (EB) was minced, and EB water, lipid, protein, ash, and energy contents were measured by chemical analyses. Prediction equations for body components measured postmortem were computed from in vivo BM and DOS. The lack of postmortem variation of fat-free EB composition was confirmed (mean of 75.3% [SD 0.6] of water), and the proportion of lipids in the EB tended ( = 0.06) to be greater for the high-energy diet (13.1%) than for the medium-energy diet (11.1%). There was a close negative relationship (residual CV [rCV] = 3.9%, = 0.957) between EB water and lipid content, whereas DOS was closely related to total body water (rCV = 2.9%, = 0.944) but DOS overestimated it by 5.8%. Adding DOS to BM improved the in vivo predictions of EB lipid and energy content (rCV = 13.1% and rCV = 7.9%, respectively) but not those of protein or ash. Accuracy of the obtained prediction equations was similar to those reported in studies determining DOS by infrared spectroscopy. Therefore, the use of IRMS to quantify DOS provides a highly accurate measure of the in vivo body composition in goat kids.
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Composición Corporal/fisiología , Agua Corporal/química , Óxido de Deuterio/metabolismo , Cabras/fisiología , Espectrometría de Masas/métodos , Tejido Adiposo/química , Animales , Óxido de Deuterio/química , Cinética , Lípidos/análisis , Masculino , Proteínas/análisisRESUMEN
The pollution of soil with the pesticide chlordecone (CLD) is a problem for the use of agricultural surfaces even years after its use has been forbidden. Therefore, the exposure of free-ranged animals such as ruminants needs to be investigated in order to assess the risk of contamination of the food chain. Indeed, measured concentrations could be integrated in a lowered extent if the soil binding would reduce the bioavailability of the pesticide. This bioavailability of soil-bound CLD in a heavily polluted andosol has been investigated relatively of CLD given via spiked oil. Twenty-four weaned lambs were exposed to graded doses of 2, 4 or 6 µg CLD/kg body weight during 15 days via the contaminated soil in comparison to spiked oil. The concentration of this pesticide has been determined in two target tissues: blood serum and kidney fat. The relative bioavailability (RBA) corresponds to the slope ratio between the test matrix-contaminated soil- in comparison to the reference matrix oil. The RBA of the soil-bound CLD was not found to significantly differ from the reference matrix oil in lambs meaning that the pesticide ingested by grazing ruminants would not be sequestered by soil binding. Therefore, CLD from soil gets bioavailable within the intestinal level and exposure to contaminated soil has to be integrated in risk assessments.
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Clordecona/metabolismo , Insecticidas/metabolismo , Ovinos/metabolismo , Contaminantes del Suelo/metabolismo , Alimentación Animal , Animales , Clordecona/análisis , Clordecona/sangre , Insecticidas/análisis , Insecticidas/sangre , Grasa Intraabdominal/metabolismo , Medición de Riesgo , Suelo/química , Contaminantes del Suelo/análisis , Contaminantes del Suelo/químicaRESUMEN
Involuntary soil intake by cows on pasture can be a potential route of entry for pollutants into the food chain. Therefore, it appears necessary to know and quantify factors affecting soil intake in order to ensure the food safety in outside rearing systems. Thus, soil intake was determined in two Latin square trials with 24 and 12 lactating dairy cows. In Trial 1, the effect of pasture allowance (20 v. 35 kg dry matter (DM) above ground level/cow daily) was studied for two sward types (pure perennial ryegrass v. mixed perennial ryegrass-white clover) in spring. In Trial 2, the effect of pasture allowance (40 v. 65 kg DM above ground level/cow daily) was studied at two supplementation levels (0 or 8 kg DM of a maize silage-based supplement) in autumn. Soil intake was determined by the method based on acid-insoluble ash used as an internal marker. The daily dry soil intake ranged, between treatments, from 0.17 to 0.83 kg per cow in Trial 1 and from 0.15 to 0.85 kg per cow in Trial 2, reaching up to 1.3 kg during some periods. In both trials, soil intake increased with decreasing pasture allowance, by 0.46 and 0.15 kg in Trials 1 and 2, respectively. In Trial 1, this pasture allowance effect was greater on mixed swards than on pure ryegrass swards (0.66 v. 0.26 kg reduction of daily soil intake between medium and low pasture allowance, respectively). In Trial 2, the pasture allowance effect was similar at both supplementation levels. In Trial 2, supplemented cows ate much less soil than unsupplemented cows (0.20 v. 0.75 kg/day, respectively). Differences in soil intake between trials and treatments can be related to grazing conditions, particularly pre-grazing and post-grazing sward height, determining at least in part the time spent grazing close to the ground. A post-grazing sward height lower than 50 mm can be considered as a critical threshold. Finally, a dietary supplement and a low grazing pressure, that is, high pasture allowance increasing post-grazing sward height, would efficiently limit the risk for high level of soil intake, especially when grazing conditions are difficult. Pre-grazing and post-grazing sward heights, as well as faecal crude ash concentration appear to be simple and practical tools for evaluating the risk for critical soil intake in grazing dairy cows.
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Crianza de Animales Domésticos/métodos , Bovinos/crecimiento & desarrollo , Conducta Alimentaria/fisiología , Suelo/análisis , Animales , Heces/química , Femenino , Inocuidad de los Alimentos , Lactancia/fisiología , Modelos EstadísticosRESUMEN
Human activities produce polluting compounds such as persistent organic pollutants (POPs), which may interact with agriculture. These molecules have raised concern about the risk of transfer through the food chain via the animal product. POPs are characterised by a strong persistence in the environment, a high volatility and a lipophilicity, which lead to their accumulation in fat tissues. These compounds are listed in international conventions to organise the information about their potential toxicity for humans and the environment. The aim of this paper is to synthesise current information on dairy ruminant exposure to POPs and the risk of their transfer to milk. Three major groups of POPs have been considered: the polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), the polychlorobiphenyls (PCBs) and the polycyclic aromatic hydrocarbons (PAHs). The results show that contamination of fodder and soil by these compounds is observed when they are exposed to emission sources (steelworks, cementworks, waste incinerators or motorways) compared with remote areas. In general, soil contamination is considered higher than plant contamination. Highest concentrations of POPs in soil may be close to 1000 ng/kg dry matter (DM) for PCDD/Fs, to 10 000 mg/kg DM for PAHs and 100 µg/kg DM for PCBs. The contamination of milk by POPs depends on environmental factors, factors related to the rearing system (fodder and potentially contaminated soil, stage of lactation, medical state of the herd) and of the characteristics of the contaminants. Transfer rates to milk have been established: for PCBs the rate of transfer varies from 5% to 90%, for PCDD/Fs from 1% to 40% and for PAHs from 0.5% to 8%. The differential transfer of the compounds towards milk is related to the hydrophobicity of the pollutants as well as to the metabolic susceptibility of the compounds.
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The aim of this study was to establish the transfer of phenanthrene, pyrene, and benzo[a]pyrene and their major hydroxylated metabolites to milk and to urine after a single oral administration (100 mg per animal of each compound) in 4 lactating goats. Detection and identification of the analytes (native compounds, 1-OH pyrene, 3-OH phenanthrene, 3-OH benzo[a]pyrene) were achieved using gas chromatography-mass spectrometry. Benzo[a]pyrene, phenanthrene, and pyrene were rapidly detected in the plasma stream, whereas 1-OH pyrene and 3-OH phenanthrene appeared later in plasma. These data suggest that pyrene and phenanthrene are progressively metabolized within the organism. Recovery rates of pyrene and phenanthrene in milk over a 24-h period appeared to be very low (0.014 and 0.006%, respectively), whereas the transfer rates of their corresponding metabolites were significantly higher: 0.44% for 1-OH pyrene and 0.073% for 3-OH phenanthrene. Recovery rates in urine were found to be higher (1 to 10 times) than recovery rates in milk. The 1-OH pyrene was found to be the main metabolite in urine as well as in milk. Thus, as has been established for humans, 1-OH pyrene could be considered as a marker of ruminant exposure to polycyclic aromatic hydrocarbons. Because 1-OH pyrene and 3-OH phenanthrene were measured in milk (unlike their corresponding native molecules), metabolites of polycyclic aromatic hydrocarbons should be taken into consideration when evaluating the safety of milk. Benzo[a]pyrene and 3-OH benzo[a]pyrene were (less than 0.005%) transferred to milk and urine in very slight amounts. This very limited transfer rate of both compounds suggests a low risk of exposure by humans to benzo[a]pyrene or its major metabolite from milk or milk products.
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Cabras/fisiología , Lactancia/metabolismo , Leche/química , Hidrocarburos Policíclicos Aromáticos/farmacocinética , Orina/química , Animales , Benzo(a)pireno/análisis , Femenino , Contaminación de Alimentos/análisis , Cromatografía de Gases y Espectrometría de Masas/métodos , Cromatografía de Gases y Espectrometría de Masas/veterinaria , Cabras/metabolismo , Hidroxilación , Fenantrenos/análisis , Hidrocarburos Policíclicos Aromáticos/sangre , Hidrocarburos Policíclicos Aromáticos/orina , Pirenos/análisisRESUMEN
Food would appear to be one of the main routes for animal and human contamination with polycyclic aromatic hydrocarbons (PAH). Many studies have shown the presence of PAH in milk and dairy products, suggesting that these foods can represent a part of this contamination. Our work aimed at defining, in vitro, the mammary barrier role in PAH transfer to milk. MAC T cells were cultivated on permeable filters to measure transepithelial permeability of 14C labeled benzo[a]pyrene (BaP), pyrene (Pyr), and phenanthrene (Phen), which differed in their physicochemical properties. The results showed that only 2 molecules, Phen and Pyr, were able to cross mammary cell layers. Phenanthrene radioactivity appeared more quickly in apical media, and its level after a 6-h exposure was 1.3 times higher than for Pyr and 7.7 times higher than for BaP. These findings suggested that mammary epithelium could play a key role in the selective transfer of PAH from food to milk.
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Glándulas Mamarias Animales/metabolismo , Leche/metabolismo , Hidrocarburos Policíclicos Aromáticos/metabolismo , Animales , Benzo(a)pireno/metabolismo , Radioisótopos de Carbono , Bovinos , Línea Celular , Permeabilidad de la Membrana Celular , Epitelio/metabolismo , Femenino , Cinética , Leche/química , Fenantrenos/metabolismo , Hidrocarburos Policíclicos Aromáticos/análisis , Pirenos/metabolismoRESUMEN
A continuous daily oral dose of (99)TcO(4)(-) (5 Bq g(-1) day(-1)) was given to young rats for 56 days. In one group the intake was continued for 98 days, while in the other the intake was stopped to facilitate the determination of biological half lives in different organs and tissues. The absorbed fraction of (99)Tc increased from 0.7 to 0.9 during the experimental period (m=0.85 sigma=0.02). The whole body retained fraction (m=0.15 sigma=0.05) indicated a storage in the tissues. The liver and kidneys showed increasing concentrations of (99)Tc until a plateau was reached after 6 and 9 weeks, then a rapid decline after the intake was stopped (half life of 6 and 5 days respectively). In hair and thyroid tissue, the accumulation of (99)Tc increased linearly then declined very slowly after the intake was stopped and did not reach a plateau during the experiment. No significant amounts of (99)Tc were detected in the muscular tissues or in the walls of the digestive tract. After the contamination period, urinary and fecal excretions fell off very quickly (1 week), but significant, though low, quantities of urinary and fecal excretions were still observed 6 weeks after stopping intake.
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Tecnecio/metabolismo , Tecnecio/farmacocinética , Absorción , Administración Oral , Animales , Cabello/química , Semivida , Riñón/química , Hígado/química , Masculino , Ratas , Ratas Wistar , Tecnecio/administración & dosificación , Glándula Tiroides/química , Distribución TisularRESUMEN
To assess PAH contamination pastures, grass and soil samples have been collected from 10 m (d1), 50 m (d2), and 150 m (d3) perpendicular to a French highway (70,000 vehicles per day) and at a control site in a rural area away from nearby contaminating sources. Total PAH concentration ranges from 767 ng/g dry weight to 3989 ng/g dry weight, according to the matrix and the distance from the highway. Distance is not a significant factor for PAH deposition on grass, while in soil it has an effect between d1 and d2 or d3. The total PAH concentration in highway samples is 8 times higher than in control site samples for grass and 7 to 4 times higher for soil. Fluoranthene, pyrene, and phenanthrene are the major PAHs in grass samples at the control site and the highway, but the concentrations are about 5 times higher near the highway. In soil samples collected near the highway, the values of concentrations between all compounds are not statistically different. PAH deposition on grass is linked to the physicochemical properties of the compounds, which lead to a specific distribution of each molecule (according to their volatility and the number of aromatic rings) while no specific behavior is revealed in soil.
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Contaminantes Ambientales/análisis , Poaceae/química , Hidrocarburos Policíclicos Aromáticos/análisis , Suelo/análisis , Emisiones de Vehículos , AgriculturaRESUMEN
The extracellular matrix, composed mainly of collagen, is considered responsible for the residual toughness of meat. Matrix metalloproteinases (MMP) responsible for the degradation of connective tissue are found in most tissues, but their participation in meat aging has not been tested. We recently showed that skeletal muscle has multiple MMP activities, as well as regulators and tissue inhibitors of metalloproteinases. Here we present the first observations of physiologic and postmortem variation of MMP activities in muscle. Growing lambs were offered two levels of intake: hay + concentrate for lambs with high growth rate (average daily gain > 250 g) and hay only for those with low growth rate (average daily gain < 25 g). At slaughter and at 21 d of postmortem aging of longissimus and semimembranosus muscles, we studied collagen content, collagen solubility, free hydroxyproline (OH-pro), and levels of latent and active forms of a matrix metalloproteinase (MMP-2) by gelatin zymography. Our results demonstrate the presence of an active isoform of MMP-2 in lamb muscle. Its level was higher (+90%, P < 0.01) in lambs that expressed a high growth rate. Activity of MMP-2 was also present at 21 d postmortem, at levels similar to those detected at slaughter. At slaughter and at 21 d, all muscles contained latent MMP-2 and the quantity of proenzyme was greater than that present in the activated form. The levels of free OH-pro in muscles of lambs with high growth rate increased significantly (P < 0.001) over 21 d from 3.75 to 5.08% of total collagen, and this was significantly related to the level of active MMP-2 at slaughter. By contrast, the amount of free OH-pro in muscles of lambs with low growth rate was not different at 21 d (1.63% of total OH-pro) than it had been at slaughter (1.84% of total OH-pro). These results suggest that collagen degradation all the way to free amino acids occurs postmortem in muscle and that there are active MMP simultaneously present that may account for this catabolism. The growth rate of animals at slaughter influences collagen turnover in vivo, as well as postmortem collagen degradation.
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Colágeno/metabolismo , Metaloendopeptidasas/metabolismo , Músculo Esquelético/química , Músculo Esquelético/enzimología , Ovinos/metabolismo , Animales , Precursores Enzimáticos , Matriz Extracelular/enzimología , Proteínas de la Matriz Extracelular/metabolismo , Femenino , Hidroxiprolina , Masculino , Metaloproteinasa 2 de la Matriz/metabolismo , Carne/normas , Cambios Post Mortem , Ovinos/crecimiento & desarrollo , SolubilidadRESUMEN
Polychlorodibenzo-para-dioxins (PCDDs) and Polychlorodibenzofurans (PCDFs) are ubiquitous in the environment. They are mainly formed as unwanted byproducts during various chemical, industrial, and combustion processes. Thus, these pollutants can be found in the food chain. The aim of this experiment was to study the transfer of PCDD/Fs from spiked milk to arterial blood in pigs, which are considered as valid models for humans. Pigs were fed with 900 mL of milk spiked with a mixture of 17 dioxins. The levels of PCDD/Fs in the serum extracts were determined using HRGC/HRMS prior to consumption of the milk, and at 3, 5, and 7 h after milk ingestion. Concentrations of PCDD/Fs in arterial plasma increased from 3 h to 5 h and decreased at 7 h. At time point 5 h, concentrations were found between 500 and 10000 pg x g(-1) fat. The transfer ratio <
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Arterias , Benzofuranos/farmacocinética , Leche , Dibenzodioxinas Policloradas/análogos & derivados , Dibenzodioxinas Policloradas/farmacocinética , Polímeros/farmacocinética , Contaminantes del Suelo/farmacología , Porcinos/metabolismo , Absorción , Animales , Benzofuranos/administración & dosificación , Benzofuranos/sangre , Dieta , Cinética , Dibenzodioxinas Policloradas/administración & dosificación , Dibenzodioxinas Policloradas/sangre , Polímeros/administración & dosificación , Vena PortaRESUMEN
The aim of this experiment was to study 15N and amino-nitrogen (AN) portal absorption in the growing pig after ingestion of uniformly (0.2509 APE) labelled 15N milk (M), yogurt ingested just after manufacturing (Y0), yogurt stored for 21 d at 4 degrees C (Y21) and heat-treated yogurt (HY). The highest porto-arterial differences (PAD) in 15N and AN were found in the period between 30 min and 90 min after ingestion. The absorption of nitrogen from M and HY mainly occurred during the 0-120 min time period (about 70% for M and 67% for HY). For Y0 and Y21, a larger displayed absorption period over the 0-240 min time period was observed. Y0 and Y21 presented a quite similar portal absorption profile. The 15N absorption rate was close to 80% for each studied milk product, suggesting that under our experimental conditions, dairy products (M, Y0, Y21 and HY) deliver nearly the same amounts of nitrogen to the organism. AN absorption rates were around 78% with a higher variability between the milk products. These results also indicate that most of the proteins were absorbed within the 240 min postprandial period.
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Absorción Intestinal/fisiología , Leche , Nitrógeno/metabolismo , Vena Porta/metabolismo , Porcinos/metabolismo , Yogur , Animales , Digestión/fisiología , Manipulación de Alimentos , Calor , Masculino , Proteínas de la Leche/metabolismo , Modelos Animales , Nitrógeno/farmacocinética , Isótopos de NitrógenoRESUMEN
Polycyclic aromatic hydrocarbons (PAHs) are lipophilic organic pollutants occurring widely in the terrestrial environment. To study the transfer of PAHs in the food chain, pigs have been fed with milk spiked either with [(14)C]phenanthrene or with [(14)C]benzo[a]pyrene. The analysis of blood radioactivity showed that both PAHs were absorbed with a maximum concentration at 5--6 h after milk ingestion, similar to fat metabolism. The blood radioactivity then decreased to reach background levels 24 h after milk ingestion. Furthermore, the blood radioactivity was higher for phenanthrene (even if the injected load was the lowest) than for benzo[a]pyrene, in agreement with their solubility difference. These findings suggest that milk fat and PAHs were absorbed during the same time period.
Asunto(s)
Benzopirenos/administración & dosificación , Fenantrenos/administración & dosificación , Hidrocarburos Policíclicos Aromáticos/metabolismo , Animales , Disponibilidad Biológica , Isótopos de Carbono , Absorción Intestinal , Masculino , Leche/química , Hidrocarburos Policíclicos Aromáticos/análisis , Porcinos , Factores de TiempoRESUMEN
Twenty-two 4-month-old lambs were used in two experiments to assess the effects of growth rate, induced by different feeding levels, and ageing (21 days, 4°C) on non-protein nitrogen (NPN) contents and their peptide composition. Muscle (Longissimus dorsi, Semimembranosus) and sex (male, female) effects were also studied. During ageing, NPN contents increased (15-20%). This variation represented 1.6-2.8% of total nitrogen. Peptides extracted from fresh muscles were in the range of molecular weight (MW) up to 12 kDa, the majority being less than 2.4 kDa (45-48%). During ageing, peptide average MW decreased (11.5-27.6%). High growth rate increased NPN contents at slaughter (+6-14%) and during ageing. Peptide average MW and post-mortem proteolysis rate as with to NPN variations were also higher. Differences between sex were observed at the end of the ageing period. Muscle effects were observed on the peptide class from 6.8 to 3.4 kDa.
RESUMEN
Ionic strength plays an important role in post mortem muscle changes. Its increase is due to ion release during the development of rigor mortis. Twelve alpine kids were used to study the effects of chilling and meat pH on ion release. Free ions were measured in Longissimus dorsi muscle by capillary electrophoresis after water extraction. All free ion concentrations increased after death, but there were differences between ions. Temperature was not a factor affecting ion release in contrast to ultimate pH value. Three release mechanisms are believed to coexist: a passive binding to proteins, which stops as pH decreases, an active segregation which stops as ATP disappears and the production of metabolites due to anaerobic glycolysis.