Relationship between Alzheimer's disease and mitochondria coenzyme II Gene.

We aimed to investigate the relevance between Alzheimer's disease (AD) and gene mutations of mitochondrial cytochrome oxidase subunit III (COX3) and coenzyme II (ND2), and to provide genetic markers for the diagnosis of Alzheimer's disease (AD) and further provide some feasible basis for preventive treatment. Polymerase chain reaction-restriction fragment length polymorphism technique was used, and genotypes and gene frequencies were detected in 60 patients with Alzheimer's disease (AD), who meet the ICD-10 diagnostic criteria (AD group), 10 AD families and 60 normal old people (control group). (1) Gene variation on nt5460 gene locus of mitochondria ND2 of the patient group is G→A, and the variation rate is 13.3%, P=0.006 < 0.05. Gene variation G→A of the patient group perfomred statistical significance. (2) In the families, it is also found that in the gene variation of G→A, the variation rate is 33.3%, P > 0.05. There is obvious gene variation in the families, but this variation does not perform statistical significance. (3) There is no gene variation on nt9861 gene locus of mitochondria COX3 of the patient group. Gene variation of T→C is not found both in the patient group and the control group. There is possible a gene mutation of G→A on nt5460 gene locus of mitochondria ND2 of the AD patients. Although gene mutation of G→A is found in the families, it performed no statistical significance. At the same time, it is found that there is no relation between AD patients and Gene variation of T→C on nt9861 gene locus of mitochondria COX3.

Evaluation of reliability of quantum resonance spectrometer application in depression symptoms detection / 中华行为医学与脑科学杂志

ObjectiveTo evaluate reliability and psychiatric clinical value of quantum resonance spectrometer (QRS) application in depression symptoms detection.MethodsThe psychiatric symptoms of 97 cases respectively obtained from QRS test and psychiatrist check were performed comparative analysis.The detecting order and checking order was under an order of go to see doctor random.ResultsSensitivity and negative predictive value of QRS were 100％ same as psychiatrists'examination in eating disorders; also specificity and positive predictive were 100％ in depressed mood,will weaken and sleep disorders.Kappa values were all greater than 0.8 of QRS in slow thinking,depressed mood,will weaken and other symptoms.The AUC of ROC line were all greater than 0.9 of QRS in slow thinking,depressed mood,will weaken,et al.11 symptoms.ConclusionQRS could be used as secondary depression diagnostic tools,provide a new basis for clinical diagnosis.

Clinical applications of quantum resonance spectrometer in schizophrenic symptoms detection / 中华行为医学与脑科学杂志

Objective To investigate if the quantum resonance spectrometer(QRS) can detect the schizophrenic symptoms in clinical applications.Methods According to the result of QRS test and ICD-10 diagnostic criteria and exclusion criteria, subjects were divided into patient( n = 65 ) and control groups( n = 100).11 schizophrenia-related symptoms were detected by QRS.Data was analyzed using SPSS13.0 statistical software statistical significance was set at P＜0.05.Results Sensitivity and specificity of QRS in the schizophrenic symptoms detection respective were hallucinations (0.887,0.851 ), lenovo undisciplined (0.847,0.868 ), thought insertion (0.800,0.843 ), logical thinking perversion ( 0.813,0.879 ), delusions ( 0.873,0.884 ), apathy ( 0.603,0.891 ), will decrease (0.883,0.886), environmental disorientation ( 0.574,0.837 ), self-disorientation ( 0.609,0.802) , double disorientation (0.603,0.843 ) and insight (0.862,0.869).Conclusion QRS detection possess higher authenticity, consistency and the high diagnostic value in the schizophrenic symptoms, special in hallucinations,thought disorder,insight symptoms.

The influence of circadian rhythm, gender and strain on tail suspension test using Kunming, BALB/C and C57BL/6 mice / 中华行为医学与脑科学杂志

Objective To investigate the influence of circadian rhythm,gender and stain on the tail suspension test in mice.Methods The immobility time of male and female Kunming,BALB/C and C57BL/6 mice in daytime or night were analyzed.Results ① The immobility time of Kunming mice during the day ( ( 114.24 ±11.18)s) was significantly more than that at night ( (65.39 ± 19.17)s).② The immobility time of male BALB/C mice( (68.57 ± 11.27 ) s) was significantly less than that of female BALB/C mice( ( 113.33 ± 3.87 ) s).③ The immobility time of C57BL/6 mice was significant more than that of Kunming and BALB/C mice under the same condition.Conclusions Circadian rhythm,gender and strain could significantly affect the immobility time of mice in tail suspension test.To increase the sensitivity and reliability of the tail suspension test,male Kunming and female BALB/C mice should be tested during the daytime.Compared to Kunming and BALB/C mice,the results of C57BL/6 mice were more stable.