RESUMEN
Gastric cancer and adenocarcinoma of the esophagogastric junction are major challenges to global public health due to their high morbidity and mortality. Despite continuous improvements in treatment techniques, patient prognosis is still affected by multiple factors. The preoperative prognostic nutritional index (PNI), a simple clinical indicator, has received widespread attention in recent years. Fiflis et al conducted a systematic review and reported that a high PNI was associated with significantly better survival in patients with gastric cancer. They also found that the PNI had prognostic value in patients with cancer of different TNM stages and had a positive effect even in advanced gastric cancer patients. Although the study did not address the impact of treatment regimens and had limited data sources, the results support the validity of the PNI as a biomarker for predicting the survival of gastric cancer patients. Future studies should further standardize the calculation method of the PNI, explore its applicability in different populations, and integrate other clinical parameters to construct more accurate prediction models.
RESUMEN
To date, studies on the application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified organisms (GMOs) are stably increasing and demonstrates LAMP is a potential and promising method for on spot identification of GMOs. However, little information is known for detection of GM potato events by LAMP. In this report, we developed an optimized and visual LAMP assay with high specificity and sensitivity to rapidly amplify genomic DNA of potato EH92-527-1 within 45 min. The limit of detection of LAMP in our study is 10-fold higher than the conventional PCR. Furthermore, LAMP products can be directly observed via naked eyes by addition of SYBR Green I without gel electrophoresis analysis and PCR-based equipment. Therefore, the LAMP assay developed in this paper provides an efficient, convenient and cost-effective tool for the detection of GM potato EH92-527-1.
Asunto(s)
ADN de Plantas/genética , Plantas Modificadas Genéticamente/genética , Reacción en Cadena de la Polimerasa/instrumentación , Reacción en Cadena de la Polimerasa/métodos , Solanum tuberosum/genética , Secuencia de Bases/genética , Benzotiazoles , Percepción de Color , Cartilla de ADN/genética , Enzimas de Restricción del ADN/genética , Diaminas , Electroforesis en Gel de Agar , Colorantes Fluorescentes/química , Contaminación de Alimentos/análisis , Amplificación de Genes , Límite de Detección , Compuestos Orgánicos/química , Reacción en Cadena de la Polimerasa/economía , Quinolinas , Sensibilidad y Especificidad , Temperatura , TiempoRESUMEN
Objective: The continuous foam separation was adopted to optimize the process conditions for separation of tatal saponins in Sapindus mukorossi. Methods: Orthogonal test and spectrophotometry were employed to determine the optimum conditions of foam separation using the enrichment ratio, yield, and purity of total saponins in S. mukorossi as evaluating indexes. Results: The best condition of the process was as follows: gas flow rate of 0.6 L/min, feed height of 40 cm, liquid flow rate of 35 mL/min with initial feed total saponins in S. mukorossi at concentration of 20 mg/L with pH 4.40. Conclusion: The yield and purity of the isolated total saponins in S. mukorossi were 23.64% and 90.27%, respectively. The continuous foam separation could conduct in relatively simple equipment, low energy consuming and provide the possibility to future industrial applications.
