Characterization of bergamot essential oil: chemical, microbiological and colloidal aspects.

Citrus bergamia is a citric species known as bergamot. The species is widely used due to its derivatives, such as juices, extracts, and essential oil. Specifically, the bergamot essential oil (BEO) is of great interest, with a chemical composition rich in terpenes and esters. Considering its chemical composition, bioactivity, and great economic potential, the characterization of BEO should be studied. However, this essential oil is almost unexplored in terms of a characterization associated with colloids. Chemical characterization was carried out by gas-chromatography coupled to a mass spectrometer and by gas-chromatography coupled to a flame ionization detector. Antibacterial activity against Staphylococcus aureus and Escherichia coli was carried out to confirm the bioactivity of this important essential oil. Dynamic light scattering analysis was performed to create a pattern of droplet size distribution of BEO. Major compounds of BEO were linalyl acetate, limonene, and linalool. The BEO was active against E. coli and presented a MIC value of 2.000 µg/mL, while values of MIC and MBC higher than 2.000 µg/mL were observed for S. aureus. The dynamic light scattering analysis revealed a mean hydrodynamic diameter of 65.7 ± 2.2 nm. After a 1:10 dilution it was observed reduction of mean diameter and enhancement of the percentagem of low size droplets, resepctively 44.1 ± 1.2 nm and 14.5 ± 0.5 nm (28.8 ± 1.2%). Higher droplets and reduced polydispersity index were observed after 1:100 dilution. In the present study, the chemical characterization was in accordance with the species, as the characteristic chemical markers of the species were found. Moreover, it has presented antibacterial activity as expected for the BEO. The analysis of the colloid showed a pattern of droplet size distribution following the Ostwald ripening mechanism after dilution.

Effects of a nanoemulsion with Copaifera officinalis oleoresin against monogenean parasites of Colossoma macropomum: A Neotropical Serrasalmidae.

Monogeneans are ectoparasites that may cause losses in production and productivity in the aquaculture of Colossoma macropomum. Chemotherapeutics used in aquaculture usually have major adverse effects on fish; hence, the use of essential oils has been considered advantageous, but these are not soluble in water. Thus, the use of nanostructures to enhance water solubility of compounds and improve bioactivity may be very promising. This study investigated the antiparasitic activity of nanoemulsion prepared with Copaifera officinalis oleoresin (50, 100, 150, 200 and 300 mg/L), against monogenean parasites from the gills of C. macropomum. The particle size distribution and zeta potential suggested that a potentially kinetic stable system was generated. The nanoemulsion from C. officinalis oleoresin achieved high efficacy (100%) at low concentrations (200 and 300 mg/L) after 15 min of exposure. This was the first time that a nanoemulsion was generated from C. officinalis oleoresin using a solvent-free, non-heating and low-energy method. Moreover, this was the first time that an antiparasitic against monogeneans on fish gills, based on nanoemulsion of C. officinalis oleoresin, was tested.

Nanoemulsion from essential oil of Pterodon emarginatus (Fabaceae) shows in vitro efficacy against monogeneans of Colossoma macropomum (Pisces: Serrasalmidae).

Outbreaks of diseases pose a major threat to sustainable aquaculture development worldwide. Application of herbal products to combat parasitic diseases provides an alternative approach for sustainable aquaculture. This study investigated the in vitro antiparasitic effects of an oil-in-water nanoemulsion prepared using the essential oil from Pterodon emarginatus, against monogeneans infesting Colossoma macropomum. Gill arches from C. macropomum (47.6 ± 14.5 g and 13.5 ± 1.4 cm) that were naturally parasitized by Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri were immersed in different dispersions of the P. emarginatus nanoemulsions (0, 50, 100, 200, 400 and 600 mg/L). The major compounds presented in the essential oil of P. emarginatus were ß-elemene, ß-caryophyllene and α-humulene. Characterization of these nanoemulsions showed that they have a small mean droplet size and low polydispersity index, which is concordant with stable systems. In this in vitro trial, the P. emarginatus nanoemulsion concentrations of 100, 200, 400 and 600 mg/L presented 100% helminthic efficacy against monogeneans of the gills of C. macropomum. The highest two concentrations used (400 and 600 mg/L) were seen to immobilize the parasites after only 15 min. Therefore, it would be worthwhile testing these concentrations in therapeutic baths against monogeneans of C. macropomum.

Zanthoxylum caribaeum (Rutaceae) essential oil: chemical investigation and biological effects on Rhodnius prolixus nymph.

A chemical investigation and bioassays against fifth-instar nymphae of the hematophagous insect Rhodnius prolixus, vector of Chagas disease, were conducted with the essential oil from Zanthoxylum caribaeum. The main results may be summarized as follows: (i) 54 components were identified, corresponding to 90.4% of the relative composition; sesquiterpenes (47.3%) and monoterpenes (41.2%) are the major constituents; (ii) muurola-4,5-trans-diene and isodaucene are described for the first time as chemical constituents of the essential oil from leaves of this species; (iii) topical treatment with the crude essential oil induced high levels of paralysis (from 18.88 to 33.33%) and mortality (from 80 to 98.9%) depending on the dose applied (0.5 to 5.0 µl per insect); (iv) feeding treatment with the crude essential oil also induced high levels of mortality (from 48.8 to 100%) but low levels of paralysis (from 2.22 to 7.77%) depending on the dose applied (0.5 to 5.0 µl/ml of blood); (v) in the continuous treatment, only the dose of 5.0 µl/cm(2) was able to promote statistical significant levels of mortality (63.3%) but no paralysis were detected. However in this group, occasionally, only few insects displayed malformations of legs and wings after treatment; and (vi) any treatment was able to disrupt the metamorphosis process since the low adult stage emergence observed to all groups was due the high insect mortality. These observations suggest the interference of Z. caribaeum compounds on the triatomine neuroendocrine system. The significance of these results in relation to the relevant biological events in R. prolixus as well as the possible use of insect growth regulators present in Z. caribaeum oil in integrated vector control programs against hematophagous triatomine species is herein discussed.

GWAS-based pathway analysis differentiates between fluid and crystallized intelligence.

Cognitive abilities vary among people. About 40-50% of this variability is due to general intelligence (g), which reflects the positive correlation among individuals' scores on diverse cognitive ability tests. g is positively correlated with many life outcomes, such as education, occupational status and health, motivating the investigation of its underlying biology. In psychometric research, a distinction is made between general fluid intelligence (gF) - the ability to reason in novel situations - and general crystallized intelligence (gC) - the ability to apply acquired knowledge. This distinction is supported by developmental and cognitive neuroscience studies. Classical epidemiological studies and recent genome-wide association studies (GWASs) have established that these cognitive traits have a large genetic component. However, no robust genetic associations have been published thus far due largely to the known polygenic nature of these traits and insufficient sample sizes. Here, using two GWAS datasets, in which the polygenicity of gF and gC traits was previously confirmed, a gene- and pathway-based approach was undertaken with the aim of characterizing and differentiating their genetic architecture. Pathway analysis, using genes selected on the basis of relaxed criteria, revealed notable differences between these two traits. gF appeared to be characterized by genes affecting the quantity and quality of neurons and therefore neuronal efficiency, whereas long-term depression (LTD) seemed to underlie gC. Thus, this study supports the gF-gC distinction at the genetic level and identifies functional annotations and pathways worthy of further investigation.

Human cognitive ability is influenced by genetic variation in components of postsynaptic signalling complexes assembled by NMDA receptors and MAGUK proteins.

Differences in general cognitive ability (intelligence) account for approximately half of the variation in any large battery of cognitive tests and are predictive of important life events including health. Genome-wide analyses of common single-nucleotide polymorphisms indicate that they jointly tag between a quarter and a half of the variance in intelligence. However, no single polymorphism has been reliably associated with variation in intelligence. It remains possible that these many small effects might be aggregated in networks of functionally linked genes. Here, we tested a network of 1461 genes in the postsynaptic density and associated complexes for an enriched association with intelligence. These were ascertained in 3511 individuals (the Cognitive Ageing Genetics in England and Scotland (CAGES) consortium) phenotyped for general cognitive ability, fluid cognitive ability, crystallised cognitive ability, memory and speed of processing. By analysing the results of a genome wide association study (GWAS) using Gene Set Enrichment Analysis, a significant enrichment was found for fluid cognitive ability for the proteins found in the complexes of N-methyl-D-aspartate receptor complex; P=0.002. Replication was sought in two additional cohorts (N=670 and 2062). A meta-analytic P-value of 0.003 was found when these were combined with the CAGES consortium. The results suggest that genetic variation in the macromolecular machines formed by membrane-associated guanylate kinase (MAGUK) scaffold proteins and their interaction partners contributes to variation in intelligence.

No association of primary Sjögren's syndrome with Fcγ receptor gene variants.

The genetic background of primary Sjögren's syndrome (pSS) is partly shared with systemic lupus erythematosus (SLE). Immunoglobulin G Fc receptors are important for clearance of immune complexes. Fcγ receptor variants and gene deletion have been found to confer SLE risk. In this study, four Fcγ receptor single-nucleotide polymorphisms (SNPs) and one copy number variation (CNV) were studied. Swedish and Norwegian pSS patients (N=527) and controls (N=528) were genotyped for the Fcγ receptor gene variant FCGR2A H131R (rs1801274) by the Illumina GoldenGate assay. FCGR3A F158V (rs396991) was analysed in 488 patients and 485 controls, FCGR3B rs447536 was analysed in 471 patients and 467 controls, and FCGR3B rs448740 was analysed in 478 cases and 455 controls, using TaqMan SNP genotyping assays. FCGR3B CNV was analysed in 124 patients and 139 controls using a TaqMan copy number assay. None of the SNPs showed any association with pSS. Also, no FCGR3B CNV association was detected. The lack of association of pSS with Fcγ receptor gene variants indicates that defective immune complex clearance may not be as important in pSS pathogenesis as in SLE, and may point to important differences between SLE and pSS.

Nanoleakage distribution at adhesive-dentin interfaces in 3D.

In spite of its role in the degradation of tooth-biomaterial interfaces, reports on nanoleakage are largely inconsistent. The aim of this work was to assess nanoleakage patterns qualitatively and quantitatively in 3D, to determine the influence of direction, position, and inclination of the field-of-view. Therefore, we applied a gold-standard 3-step etch-and-rinse adhesive to bur-cut dentin surfaces, after which interface samples were sectioned, infiltrated with an ammoniacal silver-nitrate solution, and embedded by common TEM procedures. High-resolution 3D models of interfaces were then generated by FIB and electron tomography, following strict conditions determined by Monte Carlo simulations. Inverted images in FIB tomography disclosed morphological characteristics analogous to those revealed by TEM. Quantitative analysis revealed large variations in silver-nitrate uptake between 2D image projections in different directions. Furthermore, silver-nitrate fractions in individual 2D image projections were seldom related to the total 3D volumetric fraction. Electron tomography showed that inclination also affected the morphology of silver-nitrate patterns. In conclusion, conventional nanoleakage evaluation is heavily influenced by direction, position, and inclination of the field-of-view, and thus may contain artifacts.

Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests.

LipL32 is the major lipoprotein in the membrane of pathogenic leptospira. In this work, we report on the production of monoclonal antibodies (MAbs) against recombinant LipL32 (rLipL32) and on the evaluation of their potential for use as reagents in diagnostic tests for leptospirosis. The MAbs were all of the IgG(2b) isotype and reacted specifically with native LipL32 in pathogenic serovars only. MAbs reacted in the same region of the rLipL32 molecule and their affinity constant was between 5x10(7) M(-1) and 6x10(6) M(-1). These results suggest that although the MAbs cannot be used together, they are well suited for diagnostic tests of leptospirosis based on LipL32 detection.

The significance of major connectors and denture base mucosal contacts on the functional strain patterns of maxillary removable partial dentures.

The purpose of this study was to investigate the biomechanical significance of major connectors and base mucosal contacts on the mechanical behaviour of maxillary removable partial dentures in vivo. Six subjects wearing maxillary dentures retained by conical crowns were selected for the study. Reflective photoelasticity and strain gauges were used to monitor the development of strain/stress during functional loading. Loading tests were performed initially with a denture design including a palatal major connector and denture bases and then repeated after removal of the major connectors and denture base alveolar muccosa contacts. The palatal major connector and the denture bases mucosal contacts contribute significantly to the rigidity and stability of removable partial dentures retained by conical crowns.

Jaw-closing muscles: electromyographic activity of human subjects with reduced periodontal support.

The aim of this study was to compare the electromyographic (EMG) activity of jaw-closing muscles of individuals with normal and reduced periodontal bone support. Fourteen adult subjects with more than 24 remaining teeth and low levels of periodontal inflammation, were selected for the study. Subjects of the control group had 90% of the periodontal bone support left, while other subjects presented a reduction of 52% of the original periodontal bone. Chewing experiments were performed using silicone tablets, carrots and white bread. The EMG of masseter and anterior temporal muscles was performed bilaterally, using bipolar surface electrodes, and the EMG amplitude was full wave rectified, integrated and the area extension under the curve was used to access muscle activity. The results of the chewing experiments did not show any statistically significant difference in the EMG activity of the study groups, irrespective of the chewing stuff. The height of periodontal bone support did not seem to influence the electroactivity of jaw-closing muscles, indicating that reduced periodontal support might equally be able to withstand masticatory loads. Hence, individuals with reduced periodontal bone support revealed a similar EMG activity compared to subjects with normal periodontal bone support.

Accuracy, detail reproduction, and hardness of gypsum casts produced from silicone impressions treated with glow discharge.

Plasma treatment has been reported to improve the wettability of silicone impression materials. This study investigated whether plasma treatment of silicone impressions influenced the linear dimensional accuracy, detail reproducibility, and surface hardness of models produced from a type IV dental die stone. Five silicone impressions materials were used in this study. Linear dimensional accuracy and detail reproduction were tested according to ISO standards. Surface hardness was evaluated with a Vickers hardness test. The results indicated that plasma-treated impressions from Xantopren and Provil silicone impression materials had significantly smaller dimensional change than their respective controls, and the other materials were not influenced. The detail reproduction was superior in casts produced from plasma-treated impressions from President silicone. Surface hardness was not influenced by the treatment. Plasma treatment of silicone impressions has no negative influence on the tested physical properties of die stone models.

Surface properties of elastomeric impression materials.

This study investigated the wettability of commonly used elastomeric impression materials and their gypsum castability. The wettability was evaluated by determining the contact angles of drops of a CaSO4 aqueous solution on flat impression surfaces. Gypsum castability was determined by counting the number of voids formed in die stone casts made from impressions of a critical die. All specimens used in this study were exposed to saliva to simulate clinical conditions, where materials spontaneously acquire salivary origin biofilms. The results indicate that the condensation silicones and conventional addition silicones exhibited the largest contact angles and the highest number of voids. The new generation of 'hydrophilic' addition silicones had higher wettability than the conventional silicones and an improved gypsum castability, comparable to that of the polyether materials.

Effect of salivary films on the surface properties of elastomeric impression materials.

Impression materials exposed to the oral cavity are subjected to spontaneous adsorption of salivary films. The aim of this study was to evaluate the effect of adsorbed salivary films on some surface properties of one condensation, four addition silicone and one polyether impression materials. The results showed that adsorbed salivary films caused an increase in the critical surface tension and in the polarity ratios for all materials tested. A reduction in the number of voids in die stone casts was also noticed, along with an improvement in the gypsum compatibility with the impression materials tested. It can be concluded that the presence and effect of salivary films should be taken into consideration when studying the surface properties of elastomeric impression materials.

Effect of plasma treatment on the wettability of elastomeric impression materials.

This study investigated the effect of "plasma treatment" on the wettability of impression materials and void formation in die stone casts. Four addition reaction silicones, one condensation reaction silicone, and one polyether impression material were used. Wettability was determined before and after plasma treatment by contact angle registration of a saturated aqueous solution of calcium sulfate (CaSO4) on flat surfaces made from the tested impression materials. Two groups of surfaces were used for each material. The surfaces in one of the groups were exposed to saliva for 5 minutes. The same impression materials were used to produce four groups of die stone casts from a master die. Two groups were produced from impressions that had been exposed to milk. One group from the exposed and one from the bare surfaces were plasma-treated before pouring. Voids in the stone casts were counted under a stereoscopic microscope. The results indicated that after plasma treatment, the drop of CaSO4 solution completely spread over the addition type silicone surfaces. The corresponding die stone casts were found to be void free. It was concluded that plasma treatment produced high-energy impression surfaces that result in void-free die stone casts from the addition type silicone materials.

Masticatory efficiency of patients treated with implant retained fixed bridges in the upper jaw over a 2-year period.

The aim of this study was to investigate the effect of the rehabilitation of the upper jaw with a fixed bridge supported by Brånemark implants upon patient's masticatory efficiency. Five patients wearing complete dentures in the upper jaw were selected for the study. Chewing tests using Optosil tablets were performed before fixture installation, immediately after bridge connection and 2 years after bridge delivery. The results indicated that treatment with fixed bridges retained by osseointegrated implants did not give immediate improvements in masticatory efficiency. Only after the subjects had enough time to adapt to the reconstruction was an improvement in the masticatory efficiency index observed.

Surface properties and castability of elastomeric impression materials after plasma cleaning.

The wettability of impression materials is an important requirement for accurate reproduction of intraoral structures. The aims of this study were to evaluate the effect of plasma cleaning on critical surface tension and die stone castability of five silicone impression materials. Critical surface tension was calculated using a contact angle analysis according to Zisman (1964), before and after plasma cleaning. Die stone casts were produced from impressions of a master die and the area extension of the reproduction was measured planimetrically. The results showed a general increase in the critical surface tension and in the polar force component of the composite free energy for materials treated with plasma cleaning. An improvement in castability of all materials was also noted.

The orientation and direction of rods in dental enamel.

Enamel rods are commonly accepted as perpendicularly oriented to the tooth surface and this concept has influenced the design of cavity preparations. This investigation studied the orientation and direction of enamel rods at the outer third of the enamel plate. Fifteen human molars were randomly selected and sectioned in the buccolingual, mesiodistal, and coronal directions. Angulations of the rods at the outer surface were measured by use of a protractor superimposed on the micrographs taken by scanning electron microscope. The mean angulation for axial surfaces was 60 degrees. The enamel rods were consistently parallel to the long axis of the tooth with 55-degree mean angulation. The conclusion was that enamel rods are not perpendicular to the surface except at the edge of the cusps and occasionally at the cervical area.