RESUMEN
Among plant responses to environmentally induced stress modulating protein expression appears to be a key stage in inducible signaling. Our study was focused on an innovative strategy to stimulate plant stress resistance, namely, the use of targeted sequences of specific sound frequencies. The influence of acoustic stimulation on plant protein synthesis was investigated. In our study green peas, Pisum sativum, were cultured under hydric stress conditions with targeted acoustic stimulation. Acoustic sequences targeting dehydrins (DHN) which accumulate in plants in response to dehydration were studied. We experimented on pea seeding with two different sequences of sounds: the first one corresponded to DHN cognate protein and the second one was aimed at the DHN consensus sequence. Shoot elongation after pea seed germination was estimated by fresh weight gain studied in the presence of various conditions of exposure to both sequences of sounds. DHN expression in peas was quantified via ELISA tests and Western-blotting by using specific antibodies. A significant increase in fresh weight in peas grown under exposure to the DHN cognate sound sequence was observed, whereas the consensus sound sequence had no effect on growth. Moreover, the 37kDa DHN amount was increased in peas treated with the consensus acoustic sequence. These results suggest that the expression of DHN could be specifically modulated by a designed acoustic stimulus.
