RESUMEN
STUDY QUESTION: Is it possible to develop an automated annotation tool for human embryo development in time-lapse devices based on image analysis? SUMMARY ANSWER: We developed and validated an automated software for the annotation of human embryo morphokinetic parameters, having a good concordance with expert manual annotation on 701 time-lapse videos. WHAT IS KNOWN ALREADY: Morphokinetic parameters obtained with time-lapse devices are increasingly used for the assessment of human embryo quality. However, their annotation is time-consuming and can be slightly operator-dependent, highlighting the need to develop fully automated approaches. STUDY DESIGN, SIZE, DURATION: This monocentric study was conducted on 701 videos originating from 584 couples undergoing IVF with embryo culture in a time-lapse device. The only selection criterion was that the duration of the video must be over 60 h. PARTICIPANTS/MATERIALS, SETTING, METHODS: An automated morphokinetic annotation tool was developed based on gray level coefficient of variation and detection of the thickness of the zona pellucida. The detection of cellular events obtained with the automated tool was compared with those obtained manually by trained experts in clinical settings. MAIN RESULTS AND THE ROLE OF CHANCE: Although some differences were found when embryos were considered individually, we found an overall concordance between automated and manual annotation of human embryo morphokinetics from fertilization to expanded blastocyst stage (r2 = 0.92). LIMITATIONS, REASONS FOR CAUTION: These results should undergo multicentric external evaluation in order to test the overall performance of the annotation tool. Getting access to the export of 3D videos would enhance the quality of the correlation with the same algorithm and its extension to the 3D regions of interest. A technical limitation of our work lies within the duration of the video. The more embryo stages the video contains, the more information the script has to identify them correctly. WIDER IMPLICATIONS OF THE FINDINGS: Our system paves the way for high-throughput analysis of multicentric morphokinetic databases, providing new insights into the clinical value of morphokinetics as a predictor of embryo quality and implantation. STUDY FUNDING/COMPETING INTEREST(S): This study was partly funded by Finox-Gedeon Richter Forward Grant 2016 and NeXT (ANR-16-IDEX-0007). We have no conflict of interests to declare. TRIAL REGISTRATION NUMBER: N/A.
Asunto(s)
Blastocisto , Técnicas de Cultivo de Embriones , Implantación del Embrión , Desarrollo Embrionario , Humanos , Programas Informáticos , Imagen de Lapso de TiempoRESUMEN
Most achievements to engineer blood vessels are based on multiple-step manipulations such as manual sheet rolling or sequential cell seeding followed by scaffold degradation. Here, we propose a one-step strategy using a microfluidic coextrusion device to produce mature functional blood vessels. A hollow alginate hydrogel tube is internally coated with extracellular matrix to direct the self-assembly of a mixture of endothelial cells (ECs) and smooth muscle cells (SMCs). The resulting vascular structure has the correct configuration of lumen, an inner lining of ECs, and outer sheath of SMCs. These "vesseloids" reach homeostasis within a day and exhibit the following properties expected for functional vessels (i) quiescence, (ii) perfusability, and (iii) contractility in response to vasoconstrictor agents. Together, these findings provide an original and simple strategy to generate functional artificial vessels and pave the way for further developments in vascular graft and tissue engineering and for deciphering the angiogenesis process.
Asunto(s)
Vasos Sanguíneos/citología , Endotelio Vascular/citología , Células Endoteliales de la Vena Umbilical Humana/citología , Modelos Cardiovasculares , Miocitos del Músculo Liso/citología , Ingeniería de Tejidos/métodos , Alginatos/química , Vasos Sanguíneos/efectos de los fármacos , Vasos Sanguíneos/fisiología , Línea Celular , Técnicas de Cocultivo , Colágeno/química , Combinación de Medicamentos , Endotelina-1/farmacología , Endotelio Vascular/efectos de los fármacos , Endotelio Vascular/fisiología , Matriz Extracelular/química , Células Endoteliales de la Vena Umbilical Humana/efectos de los fármacos , Células Endoteliales de la Vena Umbilical Humana/fisiología , Humanos , Hidrogeles/química , Laminina/química , Técnicas Analíticas Microfluídicas , Miocitos del Músculo Liso/efectos de los fármacos , Miocitos del Músculo Liso/fisiología , Proteoglicanos/química , Andamios del Tejido , Vasoconstricción/efectos de los fármacos , Vasoconstrictores/farmacologíaRESUMEN
An aneurysmal bone cyst of fourth metacarpal which occurred in a 7-year-old girl was explored by MRI. MRI findings such as fluid-fluid levels were compared to literature. These levels are inconstant and not pathognomonic of aneurysmal bone cyst. They could occur within others bone tumors. Even if fluid-fluid levels are highly suggestive of aneurysmal bone cyst only pathological study asserts the diagnosis. MRI procedure might be a good procedure for post surgery follow up.
