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AIM: To evaluate if, and to what extent, machine learning models can capture clinically defined Stage III/IV periodontitis from self-report questionnaires and demographic data. MATERIALS AND METHODS: Self-reported measures of periodontitis, demographic data and clinically established Stage III/IV periodontitis status were extracted from two Danish population-based cohorts (The Copenhagen Aging and Midlife Biobank [CAMB] and The Danish Health Examination Survey [DANHES]) and used to develop cross-validated machine learning models for the prediction of clinically established Stage III/IV periodontitis. Models were trained using 10-fold cross-validations repeated three times on the CAMB dataset (n = 1476), and the resulting models were validated in the DANHES dataset (n = 3585). RESULTS: The prevalence of Stage III/IV periodontitis was 23.2% (n = 342) in the CAMB dataset and 9.3% (n = 335) in the DANHES dataset. For the prediction of clinically established Stage III/IV periodontitis in the CAMB cohort, models reached area under the receiver operating characteristics (AUROCs) of 0.67-0.69, sensitivities of 0.58-0.64 and specificities of 0.71-0.80. In the DANHES cohort, models derived from the CAMB cohort achieved AUROCs of 0.64-0.70, sensitivities of 0.44-0.63 and specificities of 0.75-0.84. CONCLUSIONS: Applying cross-validated machine learning algorithms to demographic data and self-reported measures of periodontitis resulted in models with modest capabilities for the prediction of Stage III/IV periodontitis in two Danish cohorts.
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The AB0 blood group has been linked to ischaemic heart disease, stroke, and periodontal disease, while the Lewis blood group has been linked to ischaemic heart disease and obesity, all of which have been associated with periodontitis. AB0 or Lewis blood group phenotype may therefore constitute common hereditary components predisposing to these disorders. In this study, we investigated if blood group phenotype associated with periodontitis in a subpopulation consisting of 702 participants from a Danish cross-sectional cohort and, secondarily, attempted to confirm their association with hypertension, ischaemic heart disease, stroke, and obesity. No significant association between blood group phenotype and periodontitis was detected, nor were previously reported associations between blood group phenotype and hypertension, ischaemic heart disease, stroke, and obesity confirmed. This may, at least partly, be attributed to differences in study type, outcome definitions, cohort sizes, and population attributable factors. However, our results suggested a strong association between self-reported stroke and the Lewis (a-b-) phenotype (P = 0.0002, OR: 22.28; CI 95: 4.72-131.63).
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Sistema del Grupo Sanguíneo ABO/genética , Enfermedades Cardiovasculares/genética , Antígenos del Grupo Sanguíneo de Lewis/genética , Periodontitis/genética , Enfermedades Cardiovasculares/sangre , Estudios de Cohortes , Femenino , Humanos , Masculino , Persona de Mediana Edad , Obesidad/sangre , Obesidad/genética , Periodontitis/sangre , Fenotipo , Factores de Riesgo , Accidente Cerebrovascular/sangre , Accidente Cerebrovascular/genéticaRESUMEN
OBJECTIVE: The purpose of this study was to compare the microbiota of stimulated whole saliva samples from patients with severe hyposalivation to samples from individuals with normal whole saliva flow rates. It was hypothesized that the two groups differ with regard to salivary bacterial profiles. METHODS: This cross-sectional study included 36 participants (24 females and 12 males, mean age 58.5 years) with severe hyposalivation and 36 gender-, age-, and geographically matched participants with normal salivary secretion from the Danish Health Examination Survey (DANHES). The microbiota of stimulated whole saliva samples was characterized by HOMINGS. RESULTS: The two groups had comparable caries experience measured by decayed, missed, filled surfaces/teeth and decayed, missed, filled root surfaces as well as active caries lesions. In addition, no single probe target was present with a significant difference in frequency or proportional presence between groups. Furthermore, data reduction by principal component analysis and correspondence analysis showed comparable bacterial community profiles between groups. CONCLUSIONS: The results indicate that the salivary bacterial profiles of patients with severe hyposalivation do not differ from those of individuals with normal salivary secretion, when there are virtually no untreated active caries lesions present in the oral cavity.
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Microbiota , Saliva/microbiología , Xerostomía/microbiología , Anciano , Estudios de Casos y Controles , Estudios Transversales , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
INTRODUCTION: Lifestyle and general diseases are important for the development of periodontitis and other diseases in the oral cavity. Therefore, knowledge on lifestyle factors must be part of the dental curriculum. However, a search for information in the literature databases gave meagre results. The aim of this study was to describe education of lifestyle in relation to diseases in the oral cavity with focus on periodontitis and to elucidate how education is practiced and reflected in dental education in the Nordic countries. MATERIALS AND METHODS: A questionnaire, which consisted of 18 questions, was sent to the chairs of the departments of periodontology in the Nordic countries. The questions concerned extent, curriculum structure, educational method, content, assessment and evaluation of the education. RESULTS: Education on lifestyle factors took place at all dental schools, but the extent, content and placement in the curriculum varied. In some schools, more than 10 lessons were scheduled; two schools had only 3-5 lessons. The education of lifestyle factors was prioritised highest in the departments of periodontology followed by cariology and general health. Despite differences in the content across the dental schools, there were also similarities. So, at all schools smoking, medication, cardiovascular diseases and diabetes type 2 had a high priority. Education of other factors such as alcohol, psychological stress, oral hygiene habits, hypotension and obesity varied. CONCLUSION: Despite the general view that understanding of odontology is considered to be rather homogenous in the Nordic countries, the education varies across the dental schools. This variation may inspire dental educators in the future planning dental curricula.
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Educación en Odontología/métodos , Estilo de Vida , Periodoncia/educación , Curriculum , Evaluación Educacional , Femenino , Humanos , Masculino , Factores de Riesgo , Países Escandinavos y Nórdicos , Encuestas y CuestionariosRESUMEN
The aim of this study was to learn whether presence of caries in an adult population was associated with a salivary bacterial profile different from that of individuals without untreated caries. Stimulated saliva samples from 621 participants of the Danish Health Examination Survey were analyzed using the Human Oral Microbe Identification Microarray technology. Samples from 174 individuals with dental caries and 447 from a control cohort were compared using frequency and levels of identified bacterial taxa/clusters as endpoints. Differences at taxon/cluster level were analyzed using Mann-Whitney's test with Benjamini-Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles. A reduced bacterial diversity was observed in samples from subjects with dental caries. Five bacterial taxa (Veillonella parvula, Veillonella atypica, Megasphaera micronuciformis, Fusobacterium periodontium and Achromobacter xylosoxidans) and one bacterial cluster (Leptotrichia sp. clones C3MKM102 and GT018_ot417/462) were less frequently found in the caries group (adjusted p value <0.01) while two bacterial taxa (Solobacterium moorei and Streptococcus salivarius) and three bacterial clusters (Streptococcus parasanguinis I and II and sp. clone BE024_ot057/411/721, Streptococcus parasanguinis I and II and sinensis_ot411/721/767, Streptococcus salivarius and sp. clone FO042_ot067/755) were present at significantly higher levels (adjusted p value <0.01). The principal component analysis displayed a marked difference in the bacterial community profiles between groups. Presence of manifest caries was associated with a reduced diversity and an altered salivary bacterial community profile. Our data support recent theories that ecological stress-induced changes of commensal microbial communities are involved in the shift from oral health to tooth decay.
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Bacterias/clasificación , Caries Dental/microbiología , Saliva/microbiología , Achromobacter denitrificans/aislamiento & purificación , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Estudios de Casos y Controles , Estudios de Cohortes , Estudios Transversales , Índice CPO , Femenino , Fusobacterium/aislamiento & purificación , Humanos , Leptotrichia/clasificación , Masculino , Megasphaera/aislamiento & purificación , Consorcios Microbianos , Persona de Mediana Edad , Periodontitis/microbiología , Fumar , Streptococcus/clasificación , Veillonella/clasificación , Adulto JovenRESUMEN
In 2005, a new curriculum was introduced at the School of Dentistry at the University of Copenhagen. Amongst many changes, the pedagogical concept behind theoretical teaching was changed. The main emphasis was previously on lectures and teacher controlled teaching, but the emphasis has now shifted to a more dialogue-based teaching style for smaller groups where the students became much more active. Thus, the learning principle was now given the pride of place. The present article focuses on the pedagogical reorganisation of the course in oral microbiology where, based on interviews with students and teachers, an evaluation is made regarding how the changes were implemented and developed. Despite the fact that the students themselves wanted to abandon the lecture-based teaching style, there was great dissatisfaction with the new dialogue-based teaching style as the students did not think that it was possible for them to take responsibility for their learning. They would much rather listen to teachers who know the material. Initially, the teachers were very surprised by the negative attitude of the students and had to change the teaching style to more traditional teaching in groups whilst still maintaining the dialogue principle to a certain degree. In this article, the need to enter into a teaching contract between students and teachers prior to introducing new pedagogical principles is discussed.
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Curriculum/tendencias , Educación en Odontología/métodos , Boca/microbiología , Rol , Estudiantes de Odontología/psicología , Dinamarca , Humanos , Entrevistas como AsuntoRESUMEN
AIM: To evaluate the effect of a disinfectant agent based on hydrogen peroxide (Sterilex Ultra, Sterilex Corporation, Maryland, USA) on the microbiological water quality in dental unit waterlines (DUWL). SETTING: Six older dental units were disinfected with Sterilex Ultra and another six units with sodium hypochlorite. The results were compared to six non-disinfected units examined during a period without patient treatment. OUTCOME MEASURE: The number of colony forming units of bacteria (cfu)/ml of water was determined according to European Standards on water quality. DESIGN: The water quality was initially determined during a six week period, and subsequently in more detail for two weeks. Finally, the effect of prolonged administration of Sterilex Ultra was investigated for six weeks. RESULTS: Instillation of Sterilex Ultra according to the recommendations of the manufacturer initially reduced the number of bacteria in DUWL to <10(2) cfu/ml. However, following daily, as well as prolonged administration of Sterilex Ultra a gradual recolonisation was observed resulting in bacterial numbers >10(4) cfu/ml in a number of units. Major differences between the number of cfu/ml in individual units were observed. CONCLUSION: Neither daily nor extended administration of Sterilex Ultra was capable of maintaining an acceptable water quality in these older dental units.
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Desinfectantes Dentales/farmacología , Equipo Dental , Control de Infección Dental/métodos , Microbiología del Agua , Bacterias/efectos de los fármacos , Recuento de Colonia Microbiana , Humanos , Compuestos Orgánicos , Hipoclorito de Sodio/farmacologíaRESUMEN
The contamination level of alginate impressions delivered to a large dental laboratory in Sweden was determined. One hundred and seven consecutive alginate impressions were included during 7 days. Samples were taken and transferred into sterile physiological saline and analysed microbiologically for colony-forming units (cfu) as well as nonhemolytic, alpha-hemolytic, and beta-hemolytic colonies. After sampling, the clinics were contacted and asked to fill in simple questionnaires about their routines of disinfecting impressions. The questionnaire study revealed that about half of the clinics had some kind of disinfection routine, while the others rinsed in running water only. Seventy-two percent of the impressions yielded growth of bacteria, with a median number of 1.3x10(2) cfu. Thirteen per cent of the samples yielded >10(3) cfu, with a maximum number of 3.4x10(4) cfu. The majority of isolates were non- and alpha-hemolytic bacteria. Growth was recorded in 61.3% of disinfected impressions, and the numbers of bacteria in disinfected and nondisinfected impressions were similar. These findings raise the question of whether impressions need to be disinfected or if proper handling and hygienic procedures are sufficient to block the possible route of infection.
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Alginatos , Materiales de Impresión Dental , Técnica de Impresión Dental/instrumentación , Contaminación de Equipos , Laboratorios Odontológicos , Bacterias/clasificación , Recuento de Colonia Microbiana , Clínicas Odontológicas/clasificación , Desinfectantes Dentales/uso terapéutico , Desinfección/métodos , Contaminación de Equipos/prevención & control , Humanos , Práctica Privada , Odontología en Salud Pública , Suecia , AguaRESUMEN
The purpose of this study was to determine the risk of microbiological transmission into the dental laboratory via impressions and dental stone models. Metal master models were contaminated with Bacillus subtilis and Streptococcus sanguis. Impressions in alginate, polyvinylsiloxane, and polyether were used, and models were cast in two brands of dental stone. Samples were taken from the impression surfaces before and after casting, and sections from the stone models were taken by a technique developed for this study. After incubation, the numbers of colonies were counted and the numbers of colony-forming units per milliliter were calculated. The reductions were statistically significant but considered to be clinically insignificant. The conclusion is that even after severe contamination, the risks to dental laboratory personnel are minimal. "Normal" hygienic procedures are recommended instead of disinfection.
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Sulfato de Calcio , Aleaciones Dentales , Revestimiento para Colado Dental , Contaminación de Equipos , Laboratorios Odontológicos , Modelos Dentales/microbiología , Acero Inoxidable , Alginatos , Bacillus subtilis/aislamiento & purificación , Bacillus subtilis/fisiología , Recuento de Colonia Microbiana , Técnica de Colado Dental , Materiales de Impresión Dental , Técnica de Impresión Dental/instrumentación , Humanos , Polivinilos , Resinas Sintéticas , Factores de Riesgo , Siloxanos , Estadísticas no Paramétricas , Streptococcus sanguis/aislamiento & purificación , Streptococcus sanguis/fisiologíaRESUMEN
AIM: To evaluate drying of the dental unit waterlines (DUWL) as a new method of controlling the bacterial biofilm therein and thereby to reduce the number of living bacteria in dental unit water. SETTING: 18 dental units were incorporated into the study. Six units constituted the experimental units, which were emptied for stagnant water in the DUWL every night; six units were chlorinated every night (positive controls), and six units were left untreated (negative controls). OUTCOME MEASURE: Water samples from the ultrasonic scaler were examined microbiologically according to the guidelines from the Danish Standard Association and the number of colony forming units (cfu) per ml of water was determined. RESULT: Drying of DUWL did not reduce the number of cfu per ml in dental unit water below the levels found in DUWL left untreated. CONCLUSION: The drying of the waterlines for about 16 hours per day during a 19-day period did not result in reduced counts of bacteria in water samples from the experimental units.
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Bacterias/aislamiento & purificación , Biopelículas , Equipo Dental/microbiología , Desinfección/métodos , Microbiología del Agua , Recuento de Colonia Microbiana , Raspado Dental/instrumentación , Desinfectantes/uso terapéutico , Humanos , Hipoclorito de Sodio/uso terapéutico , Terapia por Ultrasonido/instrumentaciónRESUMEN
As chemical disinfection of dental impressions may cause adverse effects on materials and the dental personnel this study examined disinfection by ultraviolet radiation. Alginate, addition silicone rubber and red wax contaminated by Streptococcus salivarius, Fusobacterium nucleatum and five other bacteria in different suspension media were radiated for up to 18 min, and the number of colony forming units was compared to non-radiated controls. The effect of ultraviolet radiation differed among bacterial species and depended on the organic content in the suspension. Generally, the bacterial reduction after ultraviolet radiation was below 4 log steps and thus insufficient for disinfection of dental impressions.
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Materiales de Impresión Dental , Contaminación de Equipos/prevención & control , Control de Infección Dental/métodos , Rayos Ultravioleta , Alginatos , Bacterias/efectos de la radiación , Recuento de Colonia Microbiana , Elastómeros de Silicona , CerasRESUMEN
A binding assay was developed and used to study the binding of oral streptococcus to immobilized human fibronectin, laminin, vitronectin, fibrinogen, heparin, and collagen IV. The protein binding was dependent on the broth used for bacterial growth. The binding after growth in brain heart infusion broth, trypticase soy broth, Todd-Hewitt broth, and Dulbecco's modified Eagle's medium was examined. Most of the strains were able to bind to immobilized fibronectin and laminin, and to a minor extent vitronectin. Binding was not observed on immobilized fibrinogen, collagen IV, or heparin. Measured surface hydrophobicity correlated well with the bacterial binding strength to the proteins. Streptococcal incubation with putative inhibitors indicates multiple binding mechanisms of a lectin-like and protein nature, possibly involving protein receptors.
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Adhesión Bacteriana/fisiología , Proteínas de la Matriz Extracelular/metabolismo , Streptococcus/metabolismo , Animales , Bovinos , Medios de Cultivo , Fibronectinas/metabolismo , Humanos , Laminina/metabolismo , Streptococcus/crecimiento & desarrolloRESUMEN
OBJECTIVE: It has been demonstrated that contamination of the insides of high-speed dental turbines occurs and that bacteria as well as viruses may remain infectious when expelled from such turbines during subsequent use. Consequently, it has been widely recommended that a high-speed turbine be sterilized after each patient. The purpose of this study was to evaluate the effect of steam autoclaving on a high-speed dental turbine with a contaminated turbine chamber. STUDY DESIGN: Streptococcus salivarius and endospores of Bacillus stearothermophilus were used as test organisms to determine the effectiveness of 4 different small non-vacuum autoclaves and one vacuum autoclave. RESULTS: The study demonstrated different efficiencies among the small non-vacuum autoclaves, the best showing close to a 6 log reduction of the test organisms inside the turbine chamber. When cleaning and lubrication of the high-speed dental turbine was carried out before autoclaving, this level of reduction was observed for all the examined non-vacuum autoclaves. CONCLUSIONS: It is concluded that cleaning before sterilization is essential for safe use of high-speed dental turbines and that small non-vacuum autoclaves should be carefully evaluated before being used for the reprocessing of hollow instruments such as high-speed turbines.
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Equipo Dental de Alta Velocidad/microbiología , Control de Infección Dental/métodos , Esterilización/métodos , Contaminación de Equipos/prevención & control , Geobacillus stearothermophilus/aislamiento & purificación , Humanos , Control de Infección Dental/instrumentación , Saliva/microbiología , Esporas Bacterianas/aislamiento & purificación , Vapor , Esterilización/instrumentación , Streptococcus/aislamiento & purificación , VacioRESUMEN
The article concerns the dental education at 10 different faculties in Denmark, Iceland, Norway, Sweden, and Finland. The information is based upon a questionnaire to the faculties concerning the definition, the structure and succession of the basic science subjects/courses/themes/topics in the dental curriculum, the time span of the basic science teaching, and the methods of teaching. Furthermore, there is information about integration among the basic science subjects and the coordination/integration between the basic science teaching and the clinical teaching. Finally, the teachers' educational background and the cowork with the medical education is elucidated. The main findings are: the basic science teaching is structured in different ways in the Nordic countries. In Reykjavik, Bergen, and Aarhus this teaching is subject specific, while in Copenhagen it is mainly subject specific, as it has few integrated courses. In Gothenburg, Stockholm, and Umeå, the basic science teaching consists of a mixture of integrated courses and subject specific teaching. In Helsinki, Oslo and Malmö the basic sciences are theme and topic based, and for the two last-mentioned institutions, integrated with the clinic, except at the faculty in Malmö. Here the basic science teaching is totally integrated in the clinical teaching throughout the study. Usually, the basic science teaching is placed mainly in the first part of the curriculum; however, in Umeå, Copenhagen, and Oslo, some integration takes place.
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Curriculum , Educación en Odontología , Ciencia/educación , Docentes de Odontología , Finlandia , Islandia , Países Escandinavos y Nórdicos , Encuestas y Cuestionarios , Enseñanza/métodosRESUMEN
Dental high-speed turbines and handpieces can take up and expel microorganisms during operation and thus need regular sterilization. This study established a method for validating devices used to sterilize high-speed turbines and handpieces. The air and water channels and turbine chambers were contaminated with suspensions of Streptococcus salivarius or endospores of Bacillus stearothermophilus. The effect of flushing and/or autoclaving performed by a new device combining both procedures was evaluated by counting the number of viable bacteria recovered from these devices. Further, the effect on clinically used handpieces was evaluated. In an initial experiment, the device partially reduced S. salivarius, and the endospores survived. In a second experiment, a 5 to 6 log reduction of S. salivarius in air and water channels was obtained. No growth was observed in clinically used high-speed handpieces, and both S. salivarius and endospores were eliminated from the turbine chambers. Thus, the method of validation proved capable of discriminating between different levels of bacterial reduction.
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Equipo Dental de Alta Velocidad , Esterilización/instrumentación , Aire , Recuento de Colonia Microbiana , Contaminación de Equipos/prevención & control , Diseño de Equipo , Estudios de Evaluación como Asunto , Geobacillus stearothermophilus/crecimiento & desarrollo , Calor , Humanos , Reproducibilidad de los Resultados , Esporas Bacterianas/crecimiento & desarrollo , Streptococcus/crecimiento & desarrollo , Propiedades de Superficie , Vacio , AguaRESUMEN
Cystic fibrosis (CF) patients often suffer from Pseudomonas aeruginosa lung infection yet the source of this organism is not known. In order to determine whether CF patients might be contaminated with P. aeruginosa from dental equipment, a total of 103 water samples from 25 dental sessions in Frederiksberg Municipal Oral Health Care Service were examined. Three samples (2.9%) were positive for P. aeruginosa. Three hundred and twenty-seven water samples from 82 dental sessions from various other Municipal Oral Health Services in Denmark, attended by CF patients, were also examined. Eighteen of 327 samples (5.5%) from nine sessions (11%) were positive for P. aeruginosa. In one case, genotypically identical (RFLP, pulsed-field gel electrophoresis) P. aeruginosa strains were found both in water from the dental equipment and in the CF patients sputum. This indicates a small risk for acquiring P. aeruginosa from dental sessions, which is however equal to the yearly 'natural background' incidence (1-2%) of acquisition of P. aeruginosa in our CF centre.
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Fibrosis Quística/complicaciones , Equipo Dental/efectos adversos , Contaminación de Equipos , Control de Infección Dental , Infecciones por Pseudomonas/epidemiología , Estudios de Casos y Controles , Dinamarca/epidemiología , Humanos , Infecciones por Pseudomonas/prevención & control , Esputo/microbiología , Microbiología del AguaRESUMEN
By local delivery of antibiotics to periodontal pockets, very high initial concentrations are often quickly succeeded by subinhibitory concentrations, which may facilitate development of bacterial resistance. The purpose of the present study was to investigate possible development of resistance in suspected periodontal pathogens after exposure to subinhibitory concentrations of metronidazole and minocycline. The minimal inhibitory concentration (MIC) of 18 reference strains and 12 clinical isolates was determined by a broth dilution method. Subsequently, all strains with MIC < 8 micrograms/ml were exposed to serial passage on plates containing subinhibitory and gradually increasing concentrations of antibiotics, until growth was inhibited. Initially, most strains were inhibited at < or = 0.250 microgram/ml of minocycline and < or = 0.5 microgram/ml of metronidazole, though A. actinomycetemcomitans was resistant to metronidazole. After growth at subinhibitory concentrations, 8 strains survived 1-2 x and 11 stains survived 8-32 x their initial MIC of metronidazole, growing at up to 8 micrograms/ml. All A. actinomycetemcomitans survived 8-64 x their initial MIC of minocycline, growing at > or = 2 micrograms/ml, while all other strains were inhibited at < or = 0.250 microgram/ml, corresponding to a 1-8 x increase in their initial MIC. Thus, development of resistance was observed for periodontal bacteria growing at up to 64 x their initial MIC, but the final level of resistance was moderate.
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Antibacterianos/farmacología , Bacterias Anaerobias/efectos de los fármacos , Farmacorresistencia Microbiana , Metronidazol/farmacología , Minociclina/farmacología , Bolsa Periodontal/microbiología , Aggregatibacter actinomycetemcomitans/efectos de los fármacos , Campylobacter/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Fusobacterium nucleatum/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana , Porphyromonas gingivalis/efectos de los fármacos , Prevotella intermedia/efectos de los fármacos , Pase Seriado , Resistencia a la Tetraciclina , Treponema/efectos de los fármacosRESUMEN
The microbiota associated with ligature-induced marginal inflammation around osseointegrated dental implants, ankylosed teeth, and normal control teeth was investigated in 8 cynomolgus monkeys (Macaca fascicularis). Submucosal/subgingival plaque was sampled with paper points on the day of literature placement and after 7 weeks. The samples were evaluated by phase-contrast microscopy and by cultivation on enriched non-selective and various solid media. The submucosal/subgingival flora was changed 7 weeks after ligation. The total number of cultivable bacteria and the proportions of motile rods, anaerobic Gram-negative rods, black-pigmented rods, Porphyromonas gingivalis, and Prevotella intermedia increased significantly around implants, ankylosed teeth, and normal control teeth. Except for a significantly higher proportion of anaerobic Gram-positive cocci around implants compared to ankylosed teeth and normal control teeth at the end of the study, no significant microbiological differences were observed between implants, ankylosed teeth, and normal control teeth neither at baseline nor at the end of the study. Consequently, the microbiota associated with marginal inflammation around implants, ankylosed teeth, and normal control teeth appears to be rather similar in cynomolgus monkeys.
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Implantes Dentales/microbiología , Enfermedades Periodontales/microbiología , Animales , Anquilosis/microbiología , Bacterias Anaerobias/aislamiento & purificación , Recuento de Colonia Microbiana , Implantación Dental Endoósea , Placa Dental/microbiología , Bacterias Gramnegativas/aislamiento & purificación , Bacterias Grampositivas/aislamiento & purificación , Modelos Logísticos , Macaca fascicularis , Periodontitis/microbiología , Porphyromonas gingivalis/aislamiento & purificación , Prevotella intermedia/aislamiento & purificaciónRESUMEN
Bacteria living in biofilms as dental plaque on tooth surfaces are generally more resistant to antimicrobial agents than bacteria in batch culture normally used for in vitro susceptibility testing. In order to compare the resistance of free-living and surface-grown oral bacteria, the MIC of Streptococcus sanguis 804 and ATCC 10556 to amoxicillin, doxycycline and chlorhexidine was determined by a broth dilution method. Subsequently, S. sanguis biofilms established in an in vitro flow model were perfused with the antimicrobial agents for 48 h at concentrations equal to and up to 500 times the MIC, and biofilm cell number was determined during this period. The antibiotics at the MIC did not affect the cell number of S. sanguis biofilms compared to the starting point, and only after 48 h at 500 times the MIC were the biofilm bacteria eliminated. At intermediate concentrations biofilm cell number gradually decreased. Chlorhexidine also gradually reduced biofilm cell number, but was inhibitory at concentrations closer to the MIC than was the case for the antibiotics. Thus S. sanguis in biofilms survived up to 500 times the MIC found in batch culture for up to 48 h.
Asunto(s)
Biopelículas/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Streptococcus sanguis/efectos de los fármacos , Amoxicilina/farmacología , Resistencia a la Ampicilina , Clorhexidina/farmacología , Doxiciclina/farmacología , Resistencia a la TetraciclinaRESUMEN
A group of 1:2:1 and 2:4:2 subgingival spirochetes, well characterized by transmission electron microscopy, biochemical tests, cellular fatty acid and carbohydrate analyses, and ribotyping, was recently suggested to represent new treponemal species. The present study used DNA hybridization to examine this possibility. When DNA of a representative strain (no. 16) of the 8 1:2:1 spirochetes examined was labeled by iodination, it showed, after S1 nuclease treatment, from 58 to 104% (average 76%) homology with DNA from the 1:2:1 spirochetes, 94% homology with DNA from the type strain of Treponema socranskii and of T. socranskii subsp. socranskii, i.e., ATCC 35536T, and 62% homology with DNA from T. socranskii subsp. buccale, strain ATCC 35534T. Similarly treated DNA from a representative strain (no. 3) of 8 2:4:2 spirochetes exhibited from 90 to 105% (average 97%) homology with DNA from the 2:4:2 spirochetes, and 85% and 87% homology, respectively, with DNA from Treponema denticola strains ATCC 33520 and FDC T1. There was a negligible degree of homology between the 1:2:1 and 2:4:2 spirochetes. Thus, all the 2:4:2 spirochetes belonged to T. denticola. 1:2:2 strains with DNA homology levels >70% (5 strains) belonged to T. socranskii or T. socranskii subsp. socranskii, while those with homology levels from 58 to 63% (3 strains) most likely belonged to other subspecies of T. socranskii.