RESUMEN
The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanoparticles was carried out by citrated method. A method of conjugation of the high-purity butyrylcholinesterase with gold nanoparticles was developed. It was found that the Immobilization of butyrylcholinesterase on the surface of gold nanoparticles resulted in a significant (to 23%) increase in the specific activity of the enzyme.
Asunto(s)
Butirilcolinesterasa , Compuestos de Oro/síntesis química , Nanopartículas del Metal , Butirilcolinesterasa/química , Butirilcolinesterasa/aislamiento & purificación , Estabilidad de Enzimas , Compuestos de Oro/química , Humanos , Concentración de Iones de Hidrógeno , Nanopartículas del Metal/química , Tamaño de la PartículaRESUMEN
The threat of industrial chemical accidents and terrorist attacks requires the development of safety regulations for the cleanup of contaminated surfaces. This paper presents principles and a methodology for the development of a new toxicological parameter, "relative value unit" (RVU) as the primary decontamination standard.
Asunto(s)
Liberación de Peligros Químicos , Descontaminación , Regulación Gubernamental , Salud Laboral/legislación & jurisprudencia , Terrorismo , Liberación de Peligros Químicos/legislación & jurisprudencia , Descontaminación/legislación & jurisprudencia , Descontaminación/métodos , Exposición a Riesgos Ambientales/efectos adversos , Exposición a Riesgos Ambientales/análisis , Humanos , Modelos Teóricos , Nivel sin Efectos Adversos Observados , Terrorismo/legislación & jurisprudencia , Xenobióticos/toxicidadRESUMEN
Pollution was hygienically evaluated in the area of the former chemically dangerous OAO "Kaprolaktam-Dzerzhinsk" enterprise. The soils around its production buildings were the test subject. Sampling, chemical analysis, and hygienic evaluation of the results of the analysis were made. The work done allowed the authors to plot pollution by both area and depth. The findings may be used to choose a decontamination procedure, by taking into account the degree of pollution.
