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Benzene (C6H6), while stable under ambient conditions, can become chemically reactive at high pressures and temperatures, such as under shock loading conditions. Here, we report in situ x-ray diffraction and small angle x-ray scattering measurements of liquid benzene shocked to 55 GPa, capturing the morphology and crystalline structure of the shock-driven reaction products at nanosecond timescales. The shock-driven chemical reactions in benzene observed using coherent XFEL x-rays were a complex mixture of products composed of carbon and hydrocarbon allotropes. In contrast to the conventional description of diamond, methane and hydrogen formation, our present results indicate that benzene's shock-driven reaction products consist of layered sheet-like hydrocarbon structures and nanosized carbon clusters with mixed sp2-sp3 hybridized bonding. Implications of these findings range from guiding shock synthesis of novel compounds to the fundamentals of carbon transport in planetary physics.
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Investigation of niche specialization in microbial communities is important in assessing consequences of environmental change for ecosystem processes. Ammonia oxidizing bacteria (AOB) and archaea (AOA) present a convenient model for studying niche specialization. They coexist in most soils and effects of soil characteristics on their relative abundances have been studied extensively. This study integrated published information on the influence of temperature and pH on AOB and AOA into several hypotheses, generating predictions that were tested in soil microcosms. The influence of perturbations in temperature was determined in pH 4.5, 6 and 7.5 soils and perturbations in pH were investigated at 15°C, 25°C and 35°C. AO activities were determined by analysing changes in amoA gene and transcript abundances, stable isotope probing and nitrate production. Experimental data supported major predictions of the effects of temperature and pH, but with several significant discrepancies, some of which may have resulted from experimental limitations. The study also provided evidence for unpredicted activity of AOB in pH 4.5 soil. Other discrepancies highlighted important deficiencies in current knowledge, particularly lack of consideration of niche overlap and the need to consider combinations of factors when assessing the influence of environmental change on microbial communities and their activities.
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Amoníaco/metabolismo , Archaea/metabolismo , Bacterias/metabolismo , Microbiología del Suelo , Suelo/química , Archaea/genética , Archaea/aislamiento & purificación , Bacterias/genética , Bacterias/aislamiento & purificación , Concentración de Iones de Hidrógeno , Microbiota , Nitrificación , Oxidación-Reducción , TemperaturaRESUMEN
The practice of foodborne illness outbreak investigations has evolved, shifting away from large-scale community case-control studies towards more focused case exposure assessments and sub-cluster investigations to identify contaminated food sources. Criteria to include or exclude cases are established to increase the efficiency of epidemiological analyses and traceback activities, but these criteria can also affect the investigator's ability to implicate a suspected food vehicle. A 2010 outbreak of Salmonella ser. Hvittingfoss infections associated with a chain of quick-service restaurants (Chain A) provided a useful case study on the impact of exclusion criteria on the ability to identify a food vehicle. In the original investigation, a case-control study of restaurant-associated cases and well meal companions was conducted at the ingredient level to identify a suspected food vehicle; however, 21% of cases and 22% of well meal companions were excluded for eating at Chain A restaurants more than once during the outbreak. The objective of this study was to explore how this decision affected the results of the outbreak investigation.
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Brotes de Enfermedades , Métodos Epidemiológicos , Enfermedades Transmitidas por los Alimentos/epidemiología , Infecciones por Salmonella/epidemiología , Salmonella/aislamiento & purificación , Estudios de Casos y Controles , Microbiología de Alimentos , Humanos , RestaurantesRESUMEN
Transit through the carbon liquid phase has significant consequences for the subsequent formation of solid nanocarbon detonation products. We report dynamic measurements of liquid carbon condensation and solidification into nano-onions over â½200 ns by analysis of time-resolved, small-angle X-ray scattering data acquired during detonation of a hydrogen-free explosive, DNTF (3,4-bis(3-nitrofurazan-4-yl)furoxan). Further, thermochemical modeling predicts a direct liquid to solid graphite phase transition for DNTF products ~200 ns post-detonation. Solid detonation products were collected and characterized by high-resolution electron microscopy to confirm the abundance of carbon nano-onions with an average diameter of â½10 nm, matching the dynamic measurements. We analyze other carbon-rich explosives by similar methods to systematically explore different regions of the carbon phase diagram traversed during detonation. Our results suggest a potential pathway to the efficient production of carbon nano-onions, while offering insight into the phase transformation kinetics of liquid carbon under extreme pressures and temperatures.
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OBJECTIVE: Population-based health information on urban Aboriginal populations in Canada is limited due to challenges with the identification of Aboriginal persons in existing health data sets. The main objective of the Our Health Counts (OHC) project was to work in partnership with Aboriginal stakeholders to generate a culturally relevant, representative baseline health data set for three urban Aboriginal communities in Ontario, Canada. DESIGN: Respondent-driven sampling (RDS). SETTING: Hamilton, Ontario, Canada. PARTICIPANTS: The OHC study, in partnership with the De dwa da dehs ney >s Aboriginal Health Access Centre (DAHC), recruited 554 First Nations adults living in Hamilton using RDS. RESULTS: Among First Nations adults living in Hamilton, 78% earned less than $20â 000 per year and 70% lived in the lowest income quartile neighbourhoods. Mobility and crowded living conditions were also highly prevalent. Common chronic diseases included arthritis, hypertension, diabetes and chronic obstructive pulmonary disease and rates of emergency room access were elevated. CONCLUSIONS: RDS is an effective sampling method in urban Aboriginal contexts as it builds on existing social networks and successfully identified a population-based cohort. The findings illustrate striking disparities in health determinants and health outcomes between urban First Nations individuals and the general population which have important implications for health services delivery, programming and policy development.
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Indígenas Norteamericanos , Salud de las Minorías , Encuestas y Cuestionarios , Adolescente , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Grupos Minoritarios , Ontario , Muestreo , Determinantes Sociales de la Salud , Salud Urbana , Adulto JovenRESUMEN
Soil microbial communities mediate critical ecosystem carbon and nutrient cycles. How microbial communities will respond to changes in vegetation and climate, however, are not well understood. We reciprocally transplanted soil cores from under oak canopies and adjacent open grasslands in a California oak-grassland ecosystem to determine how microbial communities respond to changes in the soil environment and the potential consequences for the cycling of carbon. Every 3 months for up to 2 years, we monitored microbial community composition using phospholipid fatty acid analysis (PLFA), microbial biomass, respiration rates, microbial enzyme activities, and the activity of microbial groups by quantifying (13)C uptake from a universal substrate (pyruvate) into PLFA biomarkers. Soil in the open grassland experienced higher maximum temperatures and lower soil water content than soil under the oak canopies. Soil microbial communities in soil under oak canopies were more sensitive to environmental change than those in adjacent soil from the open grassland. Oak canopy soil communities changed rapidly when cores were transplanted into the open grassland soil environment, but grassland soil communities did not change when transplanted into the oak canopy environment. Similarly, microbial biomass, enzyme activities, and microbial respiration decreased when microbial communities were transplanted from the oak canopy soils to the grassland environment, but not when the grassland communities were transplanted to the oak canopy environment. These data support the hypothesis that microbial community composition and function is altered when microbes are exposed to new extremes in environmental conditions; that is, environmental conditions outside of their "life history" envelopes.
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Clima , Microbiología del Suelo , Suelo/análisis , Bacterias/crecimiento & desarrollo , Bacterias/metabolismo , California , Carbono/metabolismo , Ecosistema , Ácidos Grasos/metabolismo , Hongos/crecimiento & desarrollo , Hongos/metabolismo , Análisis de Componente Principal , Temperatura , Factores de Tiempo , Agua/metabolismoRESUMEN
Soil microbial communities are closely associated with aboveground plant communities, with multiple potential drivers of this relationship. Plants can affect available soil carbon, temperature, and water content, which each have the potential to affect microbial community composition and function. These same variables change seasonally, and thus plant control on microbial community composition may be modulated or overshadowed by annual climatic patterns. We examined microbial community composition, C cycling processes, and environmental data in California annual grassland soils from beneath oak canopies and in open grassland areas to distinguish factors controlling microbial community composition and function seasonally and in association with the two plant overstory communities. Every 3 months for up to 2 years, we monitored microbial community composition using phospholipid fatty acid (PLFA) analysis, microbial biomass, respiration rates, microbial enzyme activities, and the activity of microbial groups using isotope labeling of PLFA biomarkers (13C-PLFA). Distinct microbial communities were associated with oak canopy soils and open grassland soils and microbial communities displayed seasonal patterns from year to year. The effects of plant species and seasonal climate on microbial community composition were similar in magnitude. In this Mediterranean ecosystem, plant control of microbial community composition was primarily due to effects on soil water content, whereas the changes in microbial community composition seasonally appeared to be due, in large part, to soil temperature. Available soil carbon was not a significant control on microbial community composition. Microbial community composition (PLFA) and 13C-PLFA ordination values were strongly related to intra-annual variability in soil enzyme activities and soil respiration, but microbial biomass was not. In this Mediterranean climate, soil microclimate appeared to be the master variable controlling microbial community composition and function.
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Bacterias/crecimiento & desarrollo , Bacterias/metabolismo , Ecosistema , Quercus/microbiología , Microbiología del Suelo , Biomarcadores/análisis , Biomasa , Carbono/metabolismo , Isótopos de Carbono/metabolismo , Consumo de Oxígeno , Fosfolípidos/análisis , Hojas de la Planta/fisiología , Densidad de Población , Dinámica Poblacional , Estaciones del Año , Especificidad de la Especie , Temperatura , Agua/metabolismoRESUMEN
Frequent high-amplitude redox fluctuation may be a strong selective force on the phylogenetic and physiological composition of soil bacterial communities and may promote metabolic plasticity or redox tolerance mechanisms. To determine effects of fluctuating oxygen regimens, we incubated tropical soils under four treatments: aerobic, anaerobic, 12-h oxic/anoxic fluctuation, and 4-day oxic/anoxic fluctuation. Changes in soil bacterial community structure and diversity were monitored with terminal restriction fragment length polymorphism (T-RFLP) fingerprints. These profiles were correlated with gross N cycling rates, and a Web-based phylogenetic assignment tool was used to infer putative community composition from multiple fragment patterns. T-RFLP ordinations indicated that bacterial communities from 4-day oxic/anoxic incubations were most similar to field communities, whereas those incubated under consistently aerobic or anaerobic regimens developed distinctly different molecular profiles. Terminal fragments found in field soils persisted either in 4-day fluctuation/aerobic conditions or in anaerobic/12-h treatments but rarely in both. Only 3 of 179 total fragments were ubiquitous in all soils. Soil bacterial communities inferred from in silico phylogenetic assignment appeared to be dominated by Actinobacteria (especially Micrococcus and Streptomycetes), "Bacilli," "Clostridia," and Burkholderia and lost significant diversity under consistently or frequently anoxic incubations. Community patterns correlated well with redox-sensitive processes such as nitrification, dissimilatory nitrate reduction to ammonium (DNRA), and denitrification but did not predict patterns of more general functions such as N mineralization and consumption. The results suggest that this soil's indigenous bacteria are highly adapted to fluctuating redox regimens and generally possess physiological tolerance mechanisms which allow them to withstand unfavorable redox periods.
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Bacterias/crecimiento & desarrollo , Ecosistema , Oxígeno/farmacología , Microbiología del Suelo , Clima Tropical , Aerobiosis , Anaerobiosis , Bacterias/clasificación , Bacterias/genética , Oxidación-Reducción , Polimorfismo de Longitud del Fragmento de Restricción , Árboles , AguaRESUMEN
We tested the hypothesis that frequency analysis of the anatomic zones affected by single anterior (A), posterior (P), and middle (M) cerebral artery (CA), multivessel, and watershed infarcts will disclose specific sites (peak zones) most frequently involved by each type, sites most frequently injured by multiple different types (vulnerable zones), and overlapping sites of equal relative frequency for two or more different types of infarct (equal frequency zones). We adopted precise definitions of each vascular territory. CT and MRI studies of 50 MCA, 20 ACA-MCA, three PCA-MCA, and 30 parasagittal watershed infarcts were mapped onto a standard template. Relative infarct frequencies in each zone were analyzed within and across infarct types to identify the centers and peripheries of each, vulnerable zones, and equal frequency zones. These data were then correlated with the prior analysis of 47 ACA, PCA, dual ACA-PCA, and ACA-PCA-MCA infarcts. Zonal frequency data for MCA and watershed infarcts, the sites of peak infarct frequency, the sites of vulnerability to diverse infarcts, and the overlapping sites of equal infarct frequency are tabulated and displayed in standardized format for direct comparison of different infarcts. This method successfully displays the nature, sites, and extent of individual infarct types, illustrates the shifts in zonal frequency and lesion center that attend dual and triple infarcts, and clarifies the relationships among the diverse types of infarct.
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Encéfalo/patología , Infarto de la Arteria Cerebral Media/patología , Anciano , Encéfalo/irrigación sanguínea , Femenino , Humanos , Infarto de la Arteria Cerebral Anterior/patología , Infarto de la Arteria Cerebral Posterior/patología , Imagen por Resonancia Magnética , Masculino , Tomografía Computarizada por Rayos XRESUMEN
The distribution of metal contaminants such as chromium in soils can be strongly localized by transport limitations and redox gradients within soil aggregates. Measurements of Cr(VI) diffusion and reduction to Cr(III) were obtained in soil columns representing transects into soil aggregates in order to quantify influences of organic carbon (OC) and redox potentials on Cr transport distances and microbial community composition. Shifts in characteristic redox potentials, and the extent of Cr(VI) reduction to Cr(III) were related to OC availability. Depth profiles of Cr(VI, III) obtained with micro X-ray absorption near edge structure (micro-XANES) spectroscopy reflected interdependent effects of diffusion and spatially dependent redox potentials on reduction kinetics and microbial community composition. Shallow diffusion depths (2-10 mm) and very sharply terminated diffusion fronts in columns amended with OC (80 and 800 ppm) reflected rapid increases in Cr reduction kinetics over very short (mm) distances. These results suggest that Cr contamination in soils can be restricted to the outsides of soil aggregates due to localized transport and rapid reduction and that bulk sample characterization is inadequate for understanding the controlling biogeochemical processes.
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Cromo/química , Contaminantes del Suelo/análisis , Suelo , Difusión , Cinética , Oxidación-ReducciónRESUMEN
BACKGROUND AND PURPOSE: No validated imaging landmark exists for characterizing the medial-lateral position of abnormalities at the high convexity-parasagittal region. Our understanding of the courses and deflections of the upper cerebral sulci is limited. Our purpose, therefore, was to define a frontooccipital line with reproducible anatomic relations to the upper cerebral gyri and sulci and to validate that line for use as an anatomic landmark by specific analysis of the gyral-sulcal relationships along it. METHODS: In 100 subjects of all ages, the gyri and sulci visualized on serial axial CT sections of the upper brain were traced onto a single flat surface to delineate the anatomic relationships among the midline interhemispheric fissure, the paramedian superior frontal sulci (SFS) and intraoccipital sulci (IOS), the medial surface sulci, the high convexity sulci, and the inner table of the skull. These tracings provided a template for drawing a straight, best-fit parasagittal line from the SFS to the IOS and for assessing how reproducibly key anatomic structures align along the parasagittal line. To assure the applicability of the line to MR imaging, selected relationships were retested on serial axial MR sections in the same subjects. RESULTS: The parasagittal line could be drawn in each case and showed reproducible alignment with the SFS, hand-motor area, partes marginales, pars deflections, postcentral "parentheses," distal intraparietal sulci, and IOS. In supraventricular sections, the parasagittal line separated the sulci arising along the medial surface from those arising along the convexity. CONCLUSION: Because the anatomic relationships of the parasagittal line are reproducible, it may serve as a reference line or landmark. The tendency of this line to demarcate medial sulci from convexity sulci suggests immediate application to the definition of vascular territories and vascular watersheds, a topic under active investigation.
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Encéfalo/anatomía & histología , Encéfalo/diagnóstico por imagen , Imagen por Resonancia Magnética , Tomografía Computarizada por Rayos X , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Encéfalo/fisiología , Mapeo Encefálico , Niño , Preescolar , Femenino , Lóbulo Frontal/anatomía & histología , Mano/fisiología , Humanos , Lactante , Masculino , Persona de Mediana Edad , Movimiento/fisiología , Lóbulo Occipital/anatomía & histologíaRESUMEN
Carbon accumulation in the terrestrial biosphere could partially offset the effects of anthropogenic CO2 emissions on atmospheric CO2. The net impact of increased CO2 on the carbon balance of terrestrial ecosystems is unclear, however, because elevated CO2 effects on carbon input to soils and plant use of water and nutrients often have contrasting effects on microbial processes. Here we show suppression of microbial decomposition in an annual grassland after continuous exposure to increased CO2 for five growing seasons. The increased CO2 enhanced plant nitrogen uptake, microbial biomass carbon, and available carbon for microbes. But it reduced available soil nitrogen, exacerbated nitrogen constraints on microbes, and reduced microbial respiration per unit biomass. These results indicate that increased CO2 can alter the interaction between plants and microbes in favour of plant utilization of nitrogen, thereby slowing microbial decomposition and increasing ecosystem carbon accumulation.
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Dióxido de Carbono/metabolismo , Microbiología Ambiental , Nitrógeno/metabolismo , Poaceae/metabolismo , Atmósfera , Biodegradación Ambiental , Biomasa , Suelo , Microbiología del SueloRESUMEN
To investigate the mechanisms by which slender oat (Avena barbata Pott ex Link) enhances phenanthrene biodegradation, we analyzed the impacts of root exudates and root debris on phenanthrene biodegradation and degrader community dynamics. Accelerated phenanthrene biodegradation rates occurred in soils amended with slender oat root exudates as well as combined root debris + root exudate as compared with unamended controls. Root exudates significantly enhanced phenanthrene biodegradation in rhizosphere soils, either by increasing contaminant bioavailability and/or increasing microbial population size and activity. A modified most probable number (MPN) method was used to determine quantitative shifts in heterotrophic and phenanthrene degrader communities. During the first 4 to 6 d of treatment, heterotrophic populations increased in all amended soils. Both root debris-amended and exudate-amended soil then maintained larger phenanthrene degrader populations than in control soils later in the experiment after much of the phenanthrene had been utilized. Thus, root amendments had a greater impact over time on phenanthrene degraders than heterotrophs resulting in selective maintenance of degrader populations in amended soils compared with controls.
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Avena/fisiología , Fenantrenos/metabolismo , Microbiología del Suelo , Contaminantes del Suelo/metabolismo , Biodegradación Ambiental , Disponibilidad Biológica , Raíces de Plantas , Dinámica PoblacionalRESUMEN
We investigated the conditions promoting toluene biodegradation for gasoline-contaminated near-surface (0.6 m depth) and subsurface (4.7 to 5.0 m depth) vadose zone soils sampled from an arid environment. At both depths, water addition was required for toluene biodegradation to occur. In near-surface samples, no inorganic nutrient addition was necessary and (i) biodegradation was fastest at 0.0 MPa, (ii) biodegradation rates decreased with decreasing water potential down to ?1.0 MPa, and (iii) biodegradation was undetectable at ?1.5 MPa. For subsurface material, toluene depletion was stimulated either by slurrying with a nutrient solution or by adjusting the moisture content to 20% (0.0 MPa) with nutrient solution and lowering the oxygen concentration (to effectively 1 mg L-1 in the aqueous phase). Thus, in the subsurface material, toluene depletion was microaerobic and nutrient-limited, occurring only under low oxygen and with inorganic nutrient addition. Our studies implicate microaerophily as an important characteristic of the toluene-degrading communities in these dry soils, with soil water as a primary controller of oxygen availability.
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Sentinel node detection is an important part of the clinical management of newly diagnosed melanoma. Now there is a similar or even greater enthusiasm for sentinel node evaluation in patients with breast carcinoma. However, controversies exist regarding the dose, volume, and route of administration. Even the role of lymphoscintigraphy itself, in contrast to using only a hand-held gamma probe during surgery for sentinel node detection, is being debated. Nevertheless, many centers and surgeons find that lymphoscintigraphy images are valuable in the treatment of patients and they use lymphoscintigraphy as a guide during surgery and to confirm the results obtained with the hand-held probe. Centers just beginning to use lymphoscintigraphy may find the images especially useful. Given this fact, the authors wanted to define the practical and technical aspects of performing lymphoscintigraphy in patients with breast cancer and examined various methods for the optimization of the technique of image acquisition. The suggested technique is generally free of the controversies noted above and applies to most patients. It includes various maneuvers that aim to improve the rate of sentinel node visualization using the gamma camera and the accuracy of node detection. The recommendations presented here should prove useful for both those experienced and for those centers just beginning to use the technique of lymphoscintigraphy.
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Neoplasias de la Mama/patología , Ganglios Linfáticos/diagnóstico por imagen , Biopsia del Ganglio Linfático Centinela , Femenino , Humanos , Metástasis Linfática , Postura , Cintigrafía , Radiofármacos , Azufre Coloidal Tecnecio Tc 99mAsunto(s)
Obstrucción Intestinal/diagnóstico por imagen , Intestino Delgado/diagnóstico por imagen , Arterias Mesentéricas/diagnóstico por imagen , Anciano , Angiografía , Diagnóstico Diferencial , Femenino , Humanos , Intestino Delgado/irrigación sanguínea , Isquemia/diagnóstico por imagen , Masculino , Oclusión Vascular Mesentérica/diagnóstico por imagen , Venas Mesentéricas/diagnóstico por imagen , Persona de Mediana Edad , Radiografía Abdominal , Tomografía Computarizada por Rayos XRESUMEN
We examined the effect of reduced water availability on the fatty acid composition of Pseudomonas putida strain mt-2 grown in a defined medium in which the water potential was lowered with the permeating solutes NaCl or polyethylene glycol (PEG) with a molecular weight of 200 (PEG 200) or the nonpermeating solute PEG 8000. Transmission electron microscopy showed that -1.0-MPa PEG 8000-treated cells had convoluted outer membranes, whereas -1.0-MPa NaCl-treated or control cells did not. At the range of water potential (-0.25 to -1.5 MPa) that we examined, reduced water availability imposed by PEG 8000, but not by NaCl or PEG 200, significantly altered the amounts of trans and cis isomers of monounsaturated fatty acids that were present in whole-cell fatty acid extracts. Cells grown in basal medium or under the -0.25-MPa water potential imposed by NaCl or PEG 200 had a higher trans:cis ratio than -0.25-MPa PEG 8000-treated cells. As the water potential was lowered further with PEG 8000 amendments, there was an increase in the amount of trans isomers, resulting in a higher trans:cis ratio. Similar results were observed in cells grown physically separated from PEG 8000, indicating that these changes were not due to PEG toxicity. When cells grown in -1.5-MPa PEG 8000 amendments were exposed to a rapid water potential increase of 1.5 MPa or to a thermodynamically equivalent concentration of the permeating solute, NaCl, there was a decrease in the amount of trans fatty acids with a corresponding increase in the cis isomer. The decrease in the trans/cis ratio following hypoosomotic shock did not occur in the presence of the lipid synthesis inhibitor cerulenin or the growth inhibitors chloramphenicol and rifampicin, which indicates a constitutively operating enzyme system. These results indicate that thermodynamically equivalent concentrations of permeating and nonpermeating solutes have unique effects on membrane fatty acid composition.
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Ácidos Grasos/análisis , Polietilenglicoles/farmacología , Pseudomonas putida/química , Pseudomonas putida/fisiología , Cloruro de Sodio/farmacología , Medios de Cultivo , Microscopía Electrónica , Presión Osmótica , Pseudomonas putida/ultraestructura , Agua/químicaRESUMEN
We quantified the effects of matric and solute water potential on toluene biodegradation by Pseudomonas putida mt-2, a bacterial strain originally isolated from soil. Across the matric potential range of 0 to -1.5 MPa, growth rates were maximal for P. putida at -0.25 MPa and further reductions in the matric potential resulted in concomitant reductions in growth rates. Growth rates were constant over the solute potential range 0 to -1.0 MPa and lower at -1.5 MPa. First order toluene depletion rate coefficients were highest at 0.0 MPa as compared to other matric water potentials down to -1.5 MPa. Solute potentials down to -1.5 MPa did not affect first order toluene depletion rate coefficients. Total yield (protein) and carbon utilization efficiency were not affected by water potential, indicating that water potentials common to temperate soils were not sufficiently stressful to change cellular energy requirements. We conclude that for P. putida: (1) slightly negative matric potentials facilitate faster growth rates on toluene but more negative water potentials result in slower growth, (2) toluene utilization rate per cell mass is highest without matric water stress and is unaffected by solute potential, (3) growth efficiency did not differ across the range of matric water potentials 0.0 to -1.5 MPa.