Avaliação comparativa de métodos de ensaio imunoenzimático para theileria equi de amostras de soro equino do estado de São Paulo / Comparative evaluation of methods of immunoenzymatic assay for theileria equi of samples of equine serum of the state of São Paulo

A presente pesquisa teve como objetivo comparar a sensibilidade e especificidade de dois testes diagnósticos, um ELISA indireto e um ELISA competitivo,para Theileria equi,com a finalidade de disponibilizar um teste alternativo para o diagnóstico da babesiose em rebanhos equinos em território nacional. Foram avaliadas 168 amostras de soro equino (suspeitos ou não para babesiose), com idade mínima de um ano, independente de sexo, raça e manejo. Os testes foram realizados segundo especificações de cada fabricante. Os resultados obtidos foram analisados estatisticamente pelo Teste do Qui-quadrado. A concordância entre os testes foi de 97,2% entre os animais soropositivos (105 soropositivos no c-ELISA e 108 soropositivos no ELISA indireto) e 95,2% dos animais soronegativos (63 soronegativos no c-ELISA e 60 soronegativos no ELISA indireto). Não foram observadas diferenças estatisticamente significativas entre os dois testes. Concluiu-se que o teste ELISA indireto apresentou alta sensibilidade e especificidade (98,1% e 92,1%, respectivamente) para o diagnóstico de Babesiose por Theileria equi.

The present research had as objective to compare thesensitivity and specicvityof the tests of indirectELISA e competitive ELISA for Theileria equi, with purpose to evaluate an alternative test for control of babesiose in equinos flocks in domestic territory. 168 samples of equine serum had been evaluated (suspected or babesiosis does not stop), with minimum age of one year, independent of sex, race and handling. The tests had been carried through according to specifications of each manufacturer, following orientations of each one. The gotten results had been analyzed statistical by the Test of the Qui-quadrado. As result, agreement was observed enters the tests of 97,2% of soropositives animals (105 soropositives in c-ELISA and 108 soropositives in the indirect ELISA) and 95.2% of the soronegatives animals (63 soronegatives in c-ELISA and 60 soronegatives in the indirect ELISA). Statistical significant differences between Indirect and competitive test ELISA for Theileria equi had not been observed. It is concluded that indirect test ELISA showed high sensitivity and specificity (98,1% e 92,1%, respectively) for the diagnosis of Babesiosis by Theileria equi.

AVALIAÇÃO COMPARATIVA DE MÉTODOS DE ENSAIO IMUNOENZIMÁTICO PARA THEILERIA EQUI DE AMOSTRAS DE SORO EQUINO DO ESTADO DE SÃO PAULO.

A presente pesquisa teve como objetivo comparar a sensibilidade e especificidade de dois testes diagnósticos, um ELISA indireto e um ELISA competitivo,para Theileria equi,com a finalidade de disponibilizar um teste alternativo para o diagnóstico da babesiose em rebanhos equinos em território nacional. Foram avaliadas 168 amostras de soro equino (suspeitos ou não para babesiose), com idade mínima de um ano, independente de sexo, raça e manejo. Os testes foram realizados segundo especificações de cada fabricante. Os resultados obtidos foram analisados estatisticamente pelo Teste do Qui-quadrado. A concordância entre os testes foi de 97,2% entre os animais soropositivos (105 soropositivos no c-ELISA e 108 soropositivos no ELISA indireto) e 95,2% dos animais soronegativos (63 soronegativos no c-ELISA e 60 soronegativos no ELISA indireto). Não foram observadas diferenças estatisticamente significativas entre os dois testes. Concluiu-se que o teste ELISA indireto apresentou alta sensibilidade e especificidade (98,1% e 92,1%, respectivamente) para o diagnóstico de Babesiose por Theileria equi.

Avaliação comparativa de métodos de ensaio imunoenzimático para theileria equi de amostras de soro equino do estado de São Paulo / Comparative evaluation of methods of immunoenzymatic assay for theileria equi of samples of equine serum of the state of São Paulo

A presente pesquisa teve como objetivo comparar a sensibilidade e especificidade de dois testes diagnósticos, um ELISA indireto e um ELISA competitivo,para Theileria equi,com a finalidade de disponibilizar um teste alternativo para o diagnóstico da babesiose em rebanhos equinos em território nacional. Foram avaliadas 168 amostras de soro equino (suspeitos ou não para babesiose), com idade mínima de um ano, independente de sexo, raça e manejo. Os testes foram realizados segundo especificações de cada fabricante. Os resultados obtidos foram analisados estatisticamente pelo Teste do Qui-quadrado. A concordância entre os testes foi de 97,2% entre os animais soropositivos (105 soropositivos no c-ELISA e 108 soropositivos no ELISA indireto) e 95,2% dos animais soronegativos (63 soronegativos no c-ELISA e 60 soronegativos no ELISA indireto). Não foram observadas diferenças estatisticamente significativas entre os dois testes. Concluiu-se que o teste ELISA indireto apresentou alta sensibilidade e especificidade (98,1% e 92,1%, respectivamente) para o diagnóstico de Babesiose por Theileria equi.(AU)

The present research had as objective to compare thesensitivity and specicvityof the tests of indirectELISA e competitive ELISA for Theileria equi, with purpose to evaluate an alternative test for control of babesiose in equinos flocks in domestic territory. 168 samples of equine serum had been evaluated (suspected or babesiosis does not stop), with minimum age of one year, independent of sex, race and handling. The tests had been carried through according to specifications of each manufacturer, following orientations of each one. The gotten results had been analyzed statistical by the Test of the Qui-quadrado. As result, agreement was observed enters the tests of 97,2% of soropositives animals (105 soropositives in c-ELISA and 108 soropositives in the indirect ELISA) and 95.2% of the soronegatives animals (63 soronegatives in c-ELISA and 60 soronegatives in the indirect ELISA). Statistical significant differences between Indirect and competitive test ELISA for Theileria equi had not been observed. It is concluded that indirect test ELISA showed high sensitivity and specificity (98,1% e 92,1%, respectively) for the diagnosis of Babesiosis by Theileria equi.(AU)

Molecular detection of hemoplasma infection among cats from São Luís island, Maranhão, Brazil

Hemoplasmas are bacteria that infect erythrocytes, attaching to the red blood cell. There is a need for more reports of hemoplasma infection prevalence and molecular characterization among cats in Brazil since there are only few published reports. The present work aimed to detect and molecularly characterize the presence of hemotrophic mycoplasmas in domestic cats with outdoor access from São Luís, Maranhão, Brazil. Twenty cats (10%) were positive for Candidatus M. haemominutum, five (2.5%) for M. haemofelis, and four (2.%) for M. turicensis based on 16S rRNA gene PCRs. Five cats (2.5%) were co-positive for Candidatus M. haemominutum and M. haemofelis. PCR diagnosis was confirmed by sequencing; and phylogenetic analysis was based on 16S rRNA and rnpb genes.

Molecular and serological detection of Theileria equi and Babesia caballi in donkeys (Equus asinus) in Brazil.

Piroplasmosis in donkeys has been recognized as a serious problem of major economic importance, since the affected animals manifest loss of appetite and decreased working capacity. The present work is aimed at detecting infection or exposure of donkeys in São Paulo, Brazil to Theileria (T.) equi and Babesia (B.) caballi using molecular and serological approaches. EDTA-blood and serum samples were collected from 88 donkeys (Equus asinus). From 88 sampled donkeys, 65 (73.86%; 95% confidence interval, PI=63.41, 82.65) and 82 (93.2%; 95% confidence interval, PI=85.75, 97.46) animals showed IgG antibodies to T. equi (by ELISA) and B. caballi (by IFAT), respectively. Twenty-eight (31.81%; 95% confidence interval, PI=22.3, 42.61) and 18 (20.45%; 95% confidence interval, PI=12.6, 30.39) donkeys were positive to T. equi and B. caballi nested PCR assays, respectively. The results indicated that T. equi and B. caballi are prevalent among donkeys in Brazil.

Molecular detection of hemoplasma infection among cats from São Luís island, Maranhão, Brazil.

Hemoplasmas are bacteria that infect erythrocytes, attaching to the red blood cell. There is a need for more reports of hemoplasma infection prevalence and molecular characterization among cats in Brazil since there are only few published reports. The present work aimed to detect and molecularly characterize the presence of hemotrophic mycoplasmas in domestic cats with outdoor access from São Luís, Maranhão, Brazil. Twenty cats (10%) were positive for Candidatus M. haemominutum, five (2.5%) for M. haemofelis, and four (2.%) for M. turicensis based on 16S rRNA gene PCRs. Five cats (2.5%) were co-positive for Candidatus M. haemominutum and M. haemofelis. PCR diagnosis was confirmed by sequencing; and phylogenetic analysis was based on 16S rRNA and rnpb genes.

Molecular detection of hemoplasma infection among cats from São Luís island, Maranhão, Brazil

Hemoplasmas are bacteria that infect erythrocytes, attaching to the red blood cell. There is a need for more reports of hemoplasma infection prevalence and molecular characterization among cats in Brazil since there are only few published reports. The present work aimed to detect and molecularly characterize the presence of hemotrophic mycoplasmas in domestic cats with outdoor access from São Luís, Maranhão, Brazil. Twenty cats (10%) were positive for Candidatus M. haemominutum, five (2.5%) for M. haemofelis, and four (2.%) for M. turicensis based on 16S rRNA gene PCRs. Five cats (2.5%) were co-positive for Candidatus M. haemominutum and M. haemofelis. PCR diagnosis was confirmed by sequencing; and phylogenetic analysis was based on 16S rRNA and rnpb genes.