Multi-crosslinked hydrogel built with hyaluronic acid-tyramine, thiolated glycol chitosan and copper-doped bioglass nanoparticles for expediting wound healing.

The migration of fibroblasts and endothelial cells is a critical determinant of wound-healing outcomes for skin injuries. Here, hyaluronic acid-tyramine (HAT) and thiolated glycol chitosan (TGC) conjugates were combined with copper-doped bioglass (ACuBG) nanoparticles to build a novel type of multi-crosslinked hydrogel for stimulating the migration of cells, and thus, expediting wound healing. The optimally devised HAT/TGC/ACuBG gels had markedly improved strength and stiffness compared to the gels built from either HAT or TGC while showing sufficient elasticity, which contributes to stimulating the migration of fibroblasts. The sustainable release of silicon and copper ions from the gels was found to jointly induce the migration of human umbilical vein endothelial cells. The results based on mouse full-thickness skin defects demonstrated that they were able to fully restore the skin defects with formation of complete appendages within two weeks, suggesting their promising potency for use in expediting wound healing.

Strong, elastic and degradation-tolerated hydrogels composed of chitosan, silk fibroin and bioglass nanoparticles with factor-bestowed activity for bone tissue engineering.

Polymer hydrogels intended for use in bone repair need to be strong, elastic, and capable of enduring degradation. However, many natural polymer hydrogels lack these essential properties and thus, are unsuitable for bone repair applications. Here, a new type of multi-network hydrogel with improved mechanical and degradation-resistant properties has been developed for use in bone repair. The hydrogel is composed of thiolated chitosan (TCH), silk fibroin (SF), and thiolated bioglass (TBG) nanoparticles (NPs). The multi-networks are built through sulfhydryl self-crosslinking, diepoxide crosslinker-involved linkages of amino or hydroxyl groups, and enzyme-mediated phenol hydroxyl crosslinking. Additionally, mesoporous TBG NPs serve as a vehicle for loading stromal cell-derived factor-1 (SDF-1) to provide the gel with cell-recruiting activity. The formulated TCH/SF/TBG hydrogels exhibit remarkably enhanced strength, elasticity, and improved degradation tolerance compared to some gels made from only TCH or SF. Furthermore, TCH/SF/TBG gels can support the growth of seeded cells and the deposition of matrix components. Some TCH/SF/TBG gels also demonstrate the ability to release SDF-1 in an approximately linear manner for a few weeks while retaining the chemotactic properties of the released SDF-1. Overall, the multi-network hydrogel has the potential as an in situ forming material for cell-recruiting bone repair and regeneration.

Extraction optimization and screening of angiotensin-converting enzyme inhibitory peptides from Channa striatus through bioaffinity ultrafiltration coupled with LC-Orbitrap-MS/MS and molecular docking.

Channa striatus is high-protein food with many health functions. This study aimed to prepare, screen and identify the angiotensin-converting enzyme inhibition peptides (ACEIPs) from C. striatus hydrolysates by response surface methodology and bioaffinity ultrafiltration coupled with LC-Orbitrap-MS/MS and molecular docking. The optimal conditions for preparing ACEIPs were hydrolysis temperature 55 °C, hydrolysis time 3 h, pH 9, solid-liquid ratio 1:20 g/mL, and enzyme addition 5%, the ACE inhibition and molecular weight distribution of obtained hydrolysate was 54.35% and 8770-160 Da, respectively. Seven novel ACEIPs were screened through the established high-throughput screening approach, among which, EYFR and LPGPGP showed the strongest ACE inhibition with the IC50 value of 179.2 and 186.3 µM, respectively (P > 0.05). Molecular docking revealed that three and ten hydrogen bonds were formed between ACE and LPGPGP and EYFR, respectively, S1 and S2 were the major active pockets, but the major driving forces varied.