[Clinical study on hemangiomas treatment with high-intensity focused ultrasound (60 cases)].

OBJECTIVE: To explore the clinical effect and appropriate power of high-intensity focused ultrasound (HIFU) in the treatment of infant hemangioma. METHODS: 80 infants with hemangioma were randomly divided into four groups, 20 cases in each group. All cases in group A, B and C were irradiated on the lesion surface with 3-5 mm/s speed for five continuous come-and-goes using HIFU, with frequency 9 MHz, impulse 1 000 Hz and 10% scanning overlap. The power was respectively 3.5 W in group A, 4.0 W in group B and 4.5 W in group C. One session contained three times of treatment, with one month interval. The cases in group D were only followed up as control group. The therapeutic effect, ulcer and scar in irradiated region in group A, B, C were observed 6 months after the last treatment, which were synchronously compared with those in group D. RESULTS: 6 months after the last treatment, 7, 9, 8 cases were cured and 9, 8, 10 cases were almost cured, and 4, 3, 2 cases got improved in group A, B, C, respectively. The total effective rate was 100% in the three groups. While only 5 cases got improved in group D. The treatment effect in group A, B and C was obviously better than that in group D (P < 0.05), with no significant difference among the three groups statistically (P > 0.05). Ulcer and scar in irradiated skin occurred in 0,4,6,0 cases in group A, B, C and D, respectively. The incidence of ulcer and scar was obviously higher in group B and C than that in group A and D statistically (P < 0.05). CONCLUSIONS: HIFU irradiating is one of effective methods for infant hemangioma treatment. The appropriate power should not be above 3.5 W.

[Clinical analysis of cleft lip and palate repair at the same time on 468 infants].

OBJECTIVE: To study the feasibility, merit and shortcoming of simultaneously repairing cleft lip and palate during the infancy. METHODS: 468 infants with cleft lip and palate repaired simultaneously in our department from 1995 to 2004 were reviewed retrospectively, as well as the cases repaired three years before were followed up and evaluated in pronunciation. RESULTS: 468 infants with cleft lip and palate were effectively treated simultaneously, associated with 173 had complications and 1 died after operation. 94 cases appeared dyspnea (20.1%), 74 cases had ruptured incision in palate including 63 cases in uvula, 2 cases infected in clip incision, 1 case got paralytic brain for lack of oxygen during the anesthesia, 2 cases ran malignant high fever including 1 dying of it, 1 case bled excessively after iodoform gauze pulled out. Among the 168 cases followed up relative to 278 who had treated three years before, 115 cases got clear pronunciation, 48 cases were next and 5 cases' pronunciation were unclear. CONCLUSIONS: Repairing cleft lip and palate simultaneously during the infancy has high rates of dyspnea and ruptured incision in uvula, though which contributes to clear pronunciation and has other merits, and perfect anesthesia is important to success in operation.

Using chemical methods to crosslink xenogeneic heart valves: the progress of bioprosthetic heart valves / 中国医学科学院学报

Glutaraldehyde clinically is the most commonly accepted crosslinking reagent for bioprosthetic valves preparation. Glutaraldehyde-treated tissue is stable against chemical and enzymatic degradation; however, its calcification and cytotoxicity are severe. Dye-mediated photooxidation is an alternative tissue preservation method that oxidizes the protein with visible light in the presence of a suitable photosensitizer. This article reviews chemical mechanism, research progress, clinical applications future development of these two methods.

Crosslinking bovine pericardial bioprosthetic heart valves by phthalocyanine sensitized photooxidation reaction / 中国医学科学院学报

<p><b>OBJECTIVE</b>To crosslink bovine pericardial valves by phthalocyanine sensitized photooxidation reaction.</p><p><b>METHODS</b>Fresh bovine pericardial tissues were immersed in PBS solution containing 0.01% cupreous sulfonatophthalocyanine and exposed to a broad wavelength light source for 20-120 h. Using sodium dodecyl sulfate-polyarylamide gel electrophoresis (SDS-PAGE) to analyze the cross-linking extent of pericardial tissue. With the help of attenuated total reflection-fourier transform infrared (ATR-FTIR), explained the change of protein structure after photooxidation reaction. Differential scanning calorimetry (DSC) was used to measure the change of shrinkage temperature. Tissue water content experiment method was also used.</p><p><b>RESULTS</b>Photooxidized tissue was resistant to extraction when it was compared with untreated tissue and had similar crosslinking extent over glutaraldehyde-treated tissue. The three dimension structure of collagen were changed but not seriously destroyed and the model of photooxidation reaction was validated. Phthalocyanine-treated tissue was much more flexible than glutaraldehyde-treated tissue.</p><p><b>CONCLUSION</b>Bovine pericardial tissue can be successfully crosslinked by phthalocyanine-mediated photooxidation reaction.</p>