RESUMEN
Reducing greenhouse gas (GHG) emissions from agricultural ecosystems is vital to mitigate global warming. Conservation tillage is widely used in farmland management to improve soil quality; however, its effects on soil GHG emissions remain poorly understood, particularly in high-yield areas. Therefore, our study aimed to evaluate the effects of no-tillage (NT) combined with four straw-mulching levels (0 %, 33 %, 67 %, and 100 %) on GHG emission risk and the main influencing factors. We conducted in-situ observations of GHG emissions from soils under different management practices during the maize-growing season in Northeastern China. The results showed that NT0 (705.94 g m-2) reduced CO2 emissions by 18 % compared to ridge tillage (RT, 837.04 g m-2). Different straw mulching levels stimulated N2O emissions after rainfall, particularly under NT combined with 100 % straw mulching (2.89 kg ha-1), which was 45 % higher than that in any other treatments. The CH4 emissions flux among different treatments was nearly zero. Overall, straw mulching levels had no significant effect on the GHG emissions. During the growing season, soil NH4+-N (< 20 mg kg-1) remained low and decreased with the extension of growth stage, whereas soil NO3--N initially increased and then decreased. More importantly, the results of structural equation modeling indicate that: a) organic material input and soil moisture are key factors affecting CO2 emissions, b) nitrogen fertilizer and soil moisture promote N2O emissions, and c) climatic factors exert an inexorable influence on the GHG emissions process. Our conclusions emphasize the necessity of incorporating precipitation-response measures into farmland management to reduce the risk of GHG emissions.
RESUMEN
The clinicopathological value of microRNA-141-3p (miR-141-3p) and its prospective target genes in endometrial carcinoma (EC) remains unclear. The present study determined the expression level of miR-141-3p in EC via quantitative real-time PCR (RT-qPCR). RT-qPCR showed a markedly higher expression level of miR-141-3p in EC tissues than in non-EC endometrium tissues (P < 0.0001). The microarray and miRNA-seq data revealed upregulation of miR-141-3p. Integrated analysis based on 675 cases of EC and 63 controls gave a standardized mean difference of 1.737, confirmed the upregulation of miR-141-3p. The Kaplan-Meier survival curve showed that a higher expression of miR-141-3p positively corelated with a poorer prognosis. Combining the predicted targets and downregulated genes in EC, we obtained 271 target genes for miR-141-3p in EC. Two potential targets, PPP1R12A and PPP1R12B, were downregulated at both the mRNA and protein levels. This study indicates that the overexpression of miR-141-3p may play an important part in the carcinogenesis of EC. The overexpression of miR-141-3p may be a risk factor for the prognosis of patients with EC.