RESUMEN
Sarcomere structure underpins structural integrity, signaling, and force transmission in the muscle. In embryos of the frog Xenopus tropicalis, muscle contraction begins even while sarcomerogenesis is ongoing. To determine whether contractile activity plays a role in sarcomere formation in vivo, chemical tools were used to block acto-myosin contraction in embryos of the frog X. tropicalis, and Z-disc assembly was characterized in the paralyzed dicky ticker mutant. Confocal and ultrastructure analysis of paralyzed embryos showed delayed Z-disc formation and defects in thick filament organization. These results suggest a previously undescribed role for contractility in sarcomere maturation in vivo.
Asunto(s)
Actinas/metabolismo , Complejos Multiproteicos/metabolismo , Contracción Muscular , Sarcómeros/metabolismo , Aminobenzoatos/farmacología , Anestésicos/farmacología , Animales , Contracción Muscular/efectos de los fármacos , Sarcómeros/ultraestructura , XenopusRESUMEN
Lysophosphatidic acid (LPA) has wide-ranging effects on many different cell types, acting through G-protein-coupled receptors such as LPAR6. We show that Xenopus lpar6 is expressed from late blastulae and is enriched in the mesoderm and dorsal ectoderm of early gastrulae. Expression in gastrulae is an early response to FGF signalling. Transcripts for lpar6 are enriched in the neural plate of Xenopus neurulae and loss of function caused forebrain defects, with reduced expression of telencephalic markers (foxg1, emx1 and nkx2-1). Midbrain (en2) and hindbrain (egr2) markers were unaffected. Foxg1 expression requires LPAR6 within ectoderm and not mesoderm. Head defects caused by LPAR6 loss of function were enhanced by co-inhibiting FGF signalling, with defects extending into the hindbrain (en2 and egr2 expression reduced). This is more severe than expected from simple summation of individual defects, suggesting that LPAR6 and FGF have overlapping or partially redundant functions in the anterior neural plate. We observed similar defects in forebrain development in loss-of-function experiments for ENPP2, an enzyme involved in the synthesis of extracellular LPA. Our study demonstrates a role for LPA in early forebrain development.
Asunto(s)
Gástrula/embriología , Regulación del Desarrollo de la Expresión Génica/fisiología , Lisofosfolípidos/metabolismo , Receptores del Ácido Lisofosfatídico/metabolismo , Transducción de Señal/fisiología , Telencéfalo/embriología , Proteínas de Xenopus/metabolismo , Xenopus laevis/embriología , Animales , Secuencia de Bases , Cartilla de ADN/genética , Factores de Crecimiento de Fibroblastos/metabolismo , Gástrula/metabolismo , Análisis por Micromatrices , Datos de Secuencia Molecular , Placa Neural/metabolismo , Receptores del Ácido Lisofosfatídico/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADN , Proteínas de Xenopus/genéticaRESUMEN
The pipid frog Xenopus tropicalis has emerged as a powerful new model system for combining genetic and genomic analysis of tetrapod development with robust embryological, molecular, and biochemical assays. Its early development closely resembles that of its well-understood relative X. laevis, from which techniques and reagents can be readily transferred. In contrast to the tetraploid X. laevis, X. tropicalis has a compact diploid genome with strong synteny to those of amniotes. Recently, advances in high-throughput sequencing together with solution-hybridization whole-exome enrichment technology offer powerful strategies for cloning novel mutations as well as reverse genetic identification of sequence lesions in specific genes of interest. Further advantages include the wide range of functional and molecular assays available, the large number of embryos/meioses produced, and the ease of haploid genetics and gynogenesis. The addition of these genetic tools to X. tropicalis provides a uniquely flexible platform for analysis of gene function in vertebrate development.
Asunto(s)
Xenopus/genética , Crianza de Animales Domésticos , Animales , Mapeo Cromosómico , Respuesta al Choque por Frío , Criopreservación , ADN/aislamiento & purificación , Electroforesis en Gel de Poliacrilamida , Embrión no Mamífero/fisiología , Exones , Femenino , Fertilización , Estudios de Asociación Genética , Ligamiento Genético , Marcadores Genéticos , Pruebas Genéticas , Haploidia , Cariotipificación , Masculino , Mutagénesis , Mutación , Fenotipo , Polimorfismo Genético , Genética Inversa , Análisis de Secuencia de ADN , Tinción con Nitrato de Plata , EspermatozoidesRESUMEN
CD38 is a multifunctional protein possessing ADP-ribosyl cyclase activity responsible for both the synthesis and the degradation of several Ca(2+)-mobilizing second messengers. Although a variety of functions have been ascribed to CD38, such as immune responses, insulin secretion, and social behavior in adults, nothing is known of its role during embryonic development when Ca(2+) signals feature prominently. Here, we report the identification and functional expression of CD38 from Xenopus laevis, a key model organism for the study of vertebrate development. We show that CD38 expression and endogenous ADP-ribosyl cyclase activity are developmentally regulated during cellular differentiation. Chemical or molecular inhibition of CD38 abolished ADP-ribosyl cyclase activity and disrupted elongation of the anterior-posterior axis and differentiation of skeletal muscle, culminating in embryonic death. Our data uncover a previously unknown role for CD38 as an essential regulator of embryonic development.
Asunto(s)
ADP-Ribosil Ciclasa 1/metabolismo , Señalización del Calcio/fisiología , Calcio/metabolismo , Embrión no Mamífero/enzimología , Desarrollo Embrionario/fisiología , Regulación del Desarrollo de la Expresión Génica/fisiología , ADP-Ribosil Ciclasa 1/antagonistas & inhibidores , ADP-Ribosil Ciclasa 1/genética , Animales , Diferenciación Celular/fisiología , Embrión no Mamífero/embriología , Músculo Esquelético/embriología , Músculo Esquelético/enzimología , Xenopus laevisRESUMEN
The diploid pipid frog Xenopus tropicalis has recently emerged as a powerful new model system for combining genetic and genomic analysis of tetrapod development with embryological and biochemical assays. Its early development closely resembles that of its well-understood tetraploid relative Xenopus laevis, from which techniques and reagents can be readily transferred, but its compact genome is highly syntenic with those of amniotes. Genetic approaches are facilitated by the large number of embryos produced and the ease of haploid genetics and gynogenesis.
Asunto(s)
Técnicas Genéticas , Xenopus/crecimiento & desarrollo , Xenopus/genética , Animales , Cruzamiento , Centrómero/genética , Criopreservación , ADN Complementario/genética , Embrión no Mamífero/metabolismo , Femenino , Fertilización In Vitro , Ligamiento Genético , Marcadores Genéticos/genética , Genómica , Genotipo , Haploidia , Cariotipificación , Masculino , Mutagénesis , Mutación , Fenotipo , Polimorfismo de Nucleótido Simple/genética , Reproducción Asexuada , Tinción con Nitrato de Plata , Espermatozoides/metabolismo , Recolección de Tejidos y Órganos , Xenopus/embriología , Xenopus/fisiologíaRESUMEN
BACKGROUND: The protein components of mature skeletal muscle have largely been characterized, but the mechanics and sequence of their assembly during normal development remain an active field of study. Chaperone proteins specific to sarcomeric myosins have been shown to be necessary in zebrafish and invertebrates for proper muscle assembly and function. RESULTS: The Xenopus tropicalis mutation dicky ticker results in disrupted skeletal muscle myofibrillogenesis, paralysis, and lack of heartbeat, and maps to a missense mutation in the muscle-specific chaperone unc45b. Unc45b is known to be required for folding the head domains of myosin heavy chains, and mutant embryos fail to incorporate muscle myosin into sarcomeres. Mutants also show delayed polymerization of alpha-actinin-rich Z-bodies into the Z-disks that flank the myosin-containing A-band. CONCLUSIONS: The dicky ticker phenotype confirms that a requirement for myosin-specific chaperones is conserved in tetrapod sarcomerogenesis, and also suggests a novel role for myosin chaperone function in Z-body maturation.
Asunto(s)
Chaperonas Moleculares/genética , Chaperonas Moleculares/metabolismo , Mutación Missense , Xenopus/embriología , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Animales , Genes Letales , Datos de Secuencia Molecular , Desarrollo de Músculos , Miofibrillas/metabolismo , Miosinas/metabolismo , Sarcómeros/metabolismo , Alineación de Secuencia , Xenopus/metabolismo , Pez CebraRESUMEN
Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398-1406, 2009. (c) 2009 Wiley-Liss, Inc.
Asunto(s)
Mapeo Cromosómico/métodos , Cromosomas , Mutación , Xenopus/genética , Animales , Centrómero/genética , Femenino , Marcadores Genéticos , Masculino , Meiosis/fisiología , Recombinación GenéticaRESUMEN
The precise localization of gene expression within the developing embryo, and how it changes over time, is one of the most important sources of information for elucidating gene function. As a searchable resource, this information has up until now been largely inaccessible to the Xenopus community. Here, we present a new database of Xenopus gene expression patterns, queryable by specific location or region in the embryo. Pattern matching can be driven either from an existing in situ image, or from a user-defined pattern based on development stage schematic diagrams. The data are derived from the work of a group of 21 Xenopus researchers over a period of 4 days. We used a novel, rapid manual annotation tool, XenMARK, which exploits the ability of the human brain to make the necessary distortions in transferring data from the in situ images to the standard schematic geometry. Developmental Dynamics 238:1379-1388, 2009. (c) 2009 Wiley-Liss, Inc.
Asunto(s)
Bases de Datos Genéticas , Regulación del Desarrollo de la Expresión Génica , Expresión Génica , Xenopus laevis/embriología , Xenopus laevis/genética , Animales , Humanos , Programas Informáticos , Xenopus laevis/anatomía & histologíaRESUMEN
Bio-electrosprays, a recently pioneered direct cell engineering approach, have been demonstrated to handle living cells including stem cells for the development of active specialized and unspecialized microenvironments. This electric field driven technique is currently undergoing vigorous development where the technique is racing towards possible clinical utility. Although this direct cell engineering approach has been elucidated to have no significant effects on the processed cells from a molecular level upwards, the technique needs to demonstrate its potential for use with whole organisms (multi-cellular systems). We believe this is mandatory for whole organisms such as model embryos; developing multi-cellular biological structures are sensitive systems and could possibly be prone to a wide range of embryological disruptions during their dynamic development, post-treatment. Therefore our studies presented herein have investigated the effects on embryos in terms of their structure, function and biological integrity post-bio-electrospraying in comparison to several controls. Our investigations demonstrate the absence of any detectable gross effects on the embryos from a genetic level upwards on post-treated embryos. In fact, these studies clearly elucidate no significant disruptions on the dynamic development of these treated embryos in comparison to those respective controls, thus validating the utility of bio-electrosprays for the careful handling of dynamically developing multi-cellular organisms.
Asunto(s)
Modelos Animales , Xenopus/embriología , Animales , Manejo de Especímenes/métodos , Técnicas de Cultivo de TejidosRESUMEN
The sea urchin is an extensively used model system for the study of calcium signalling by the messenger molecules NAADP and cyclic ADP-ribose. Both are synthesized by ADP-ribosyl cyclases but our molecular understanding of these enzymes in the sea urchin is limited. We have recently reported the cloning of an extended family of sea urchin ADP-ribosyl cyclases and shown that one of these enzymes (SpARC1) is active within the endoplasmic reticulum lumen. These studies suggest that production of messengers is compartmentalized. Here we characterize the properties of SpARC2. SpARC2 catalyzed both NAADP and cyclic ADP-ribose production. Unusually, the NAD surrogate, NGD was a poor substrate. In contrast to SpARC1, heterologously expressed SpARC2 localized to the plasma membrane via a glycosylphosphatidylinositol (GPI)-anchor. Transcripts for SpARC2 were readily detectable in sea urchin eggs and a majority of the endogenous membrane bound activity was found to be GPI-anchored. Our data reveal striking differences in the properties of sea urchin ADP-ribosyl cyclases and provide further evidence that messenger production may occur outside of the cytosol.
Asunto(s)
ADP-Ribosil Ciclasa/metabolismo , Erizos de Mar/enzimología , ADP-Ribosil Ciclasa/genética , ADP-Ribosil Ciclasa/inmunología , Animales , Secuencia de Bases , Células Cultivadas , ADP-Ribosa Cíclica/biosíntesis , Humanos , Microscopía Fluorescente , NADP/análogos & derivados , NADP/biosíntesis , Oocitos/enzimología , Transfección , Fosfolipasas de Tipo C/metabolismo , Xenopus laevisRESUMEN
Xld (Xolloid) is a member of the Tolloid family of metalloproteases found in embryos of the frog Xenopus laevis. It cleaves Chordin, an inhibitory binding protein for BMP2/4, releasing fragments with reduced affinity for these important ventralizing signals. As a consequence, increasing Xld activity ventralizes Xenopus embryos. We have used this phenotype as an assay to determine the requirement for the C-terminal, nonprotease component of Xld for in vivo activity. This part of the protein is composed of five complement C1r/C1s-sea urchin epidermal growth factor-BMP1 (CUB) and two epidermal growth factor domains, which are thought to be involved in protein-protein interactions and may confer substrate specificity. Our results show that the protease coupled to CUB1 and CUB2 is the minimum domain structure required to ventralize Xenopus embryos and to block the dorsal axis-inducing activity of Chordin. Xld-CUB1-CUB2 cleaves Chordin, and a protease-inactive version co-precipitates Chordin. Our results indicate that the first and second CUB domains bind Chordin and present it to the protease domain. Protease-inactive Xld blocks the cleavage of Chordin by wild-type Xld and dorsalizes injected Xenopus embryos. We find that protease-inactive Xld-CUB1-CUB2 does not share this activity and that all of the C-terminal domains are required to generate the dorsalized phenotype.
Asunto(s)
Tipificación del Cuerpo/fisiología , Proteínas Morfogenéticas Óseas/metabolismo , Glicoproteínas/metabolismo , Péptidos y Proteínas de Señalización Intercelular/metabolismo , Metaloendopeptidasas/metabolismo , Transducción de Señal/fisiología , Proteínas de Xenopus/metabolismo , Animales , Proteína Morfogenética Ósea 2 , Proteína Morfogenética Ósea 4 , Proteínas Morfogenéticas Óseas/genética , Femenino , Glicoproteínas/genética , Péptidos y Proteínas de Señalización Intercelular/genética , Masculino , Metaloendopeptidasas/genética , Estructura Terciaria de Proteína/fisiología , Proteínas de Xenopus/genética , Xenopus laevisRESUMEN
We describe a Xenopus P2Y receptor that shares only weak homology with members of the mammalian P2Y family, being most similar to human P2Y(11). When activated by nucleotide analogs, it stimulates both calcium and cAMP mobilization pathways, a feature unique, among mammalian P2Y receptors, to P2Y(11). Activity can be blocked by compounds known to act as antagonists of mammalian P2Y(11). Genomic synteny between Xenopus and mammals suggests that the novel gene is a true ortholog of P2Y(11). Xenopus P2Y(11) is transcribed during embryonic development, beginning at gastrulation, and is enriched in the developing nervous system.
Asunto(s)
AMP Cíclico/metabolismo , Receptores Purinérgicos P2/genética , Receptores Purinérgicos P2/metabolismo , Proteínas de Xenopus/genética , Proteínas de Xenopus/metabolismo , Xenopus laevis/genética , Xenopus laevis/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Señalización del Calcio , Cartilla de ADN/genética , Regulación del Desarrollo de la Expresión Génica , Humanos , Mamíferos/genética , Datos de Secuencia Molecular , Sistemas de Mensajero Secundario , Homología de Secuencia de Aminoácido , Especificidad de la Especie , Xenopus laevis/embriologíaRESUMEN
BACKGROUND: ADP-ribosyl cyclases are remarkable enzymes capable of catalyzing multiple reactions including the synthesis of the novel and potent intracellular calcium mobilizing messengers, cyclic ADP-ribose and NAADP. Not all ADP-ribosyl cyclases however have been characterized at the molecular level. Moreover, those that have are located predominately at the outer cell surface and thus away from their cytosolic substrates. METHODOLOGY/PRINCIPAL FINDINGS: Here we report the molecular cloning of a novel expanded family of ADP-ribosyl cyclases from the sea urchin, an extensively used model organism for the study of inositol trisphosphate-independent calcium mobilization. We provide evidence that one of the isoforms (SpARC1) is a soluble protein that is targeted exclusively to the endoplasmic reticulum lumen when heterologously expressed. Catalytic activity of the recombinant protein was readily demonstrable in crude cell homogenates, even under conditions where luminal continuity was maintained. CONCLUSIONS/SIGNIFICANCE: Our data reveal a new intracellular location for ADP-ribosyl cyclases and suggest that production of calcium mobilizing messengers may be compartmentalized.
Asunto(s)
ADP-Ribosil Ciclasa/química , ADP-Ribosil Ciclasa/genética , ADP-Ribosil Ciclasa/metabolismo , Secuencia de Aminoácidos , Animales , Señalización del Calcio , Clonación Molecular , ADP-Ribosa Cíclica/metabolismo , Citosol/enzimología , Citosol/metabolismo , Retículo Endoplásmico/enzimología , Retículo Endoplásmico/metabolismo , Datos de Secuencia Molecular , NADP/análogos & derivados , NADP/metabolismo , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Erizos de Mar/enzimología , Alineación de SecuenciaRESUMEN
Lysyl oxidase (Lox) is a copper-dependent amine oxidase that catalyzes the cross-linking of collagen and elastin fibers in the extracellular matrix (ECM). In mammals, four closely related Lox-like enzymes have been described that share a highly conserved catalytic domain with Lox. We have characterized Xenopus laevis cDNAs for Lox, Loxl-1, and Loxl-3, and show that they are expressed during early embryonic development. Using RT-PCR we detected maternal transcripts for Xloxl-1, but levels remained low until tailbud stages. Transcripts for Xlox and Xloxl-3 were not detected until early neurulae, although transcripts for Xlox remained at low levels until tailbud stages. Whole mount in situ hybridization showed that transcripts for Xloxl-1 and Xloxl-3 are localized in the notochord, while transcripts for Xlox are found in the notochord, somites, and head. X. laevis Lox-like enzymes were inhibited by incubating embryos, from cleavage stages to tadpole stages, in beta-aminopropionitrile, a specific inhibitor of the catalytic domain. The resulting embryos appeared to differentiate normally but suffered from poor collagen fiber formation. Defects included kinks in the notochord, a posterior shift of the somites, abnormal gut coiling, and the formation of edemas. Our data suggest that Lox-related enzymes are required for the proper formation of the ECM during X. laevis development.