RESUMEN
Pancreatic explants from perinatal or 1-week-old rat circumfusion organ cultured with an insulin-free variant of Trowell's Medium T8 survive functionally, as judged from tissue amylase content, for about 3 days. When hydrocortisone 21-sodium succinate, 1.0 mg/liter, is added to the chemically defined medium, high levels of anylase persist for longer periods. Explants from 7-day-old animals, circumfusion cultured with hydrocortisone-supplemented medium, maintain tissue amylase levels equal to or greater than those of uncultured control pancreas for at least 5 days of culture, and over this period they release amylase into culture medium at a stable rate. Methods for maintaining functional pancreatic acinar tissue in culture provide a new biological model for in vitro analysis of the early defects of potential chemical carcinogens on this target organ.
Asunto(s)
Hidrocortisona/farmacología , Técnicas de Cultivo de Órganos , Páncreas , Amilasas/metabolismo , Animales , Animales Recién Nacidos , Medios de Cultivo , Femenino , Masculino , Páncreas/enzimología , Ratas , Estimulación Química , Factores de Tiempo , Supervivencia TisularRESUMEN
An investigational red cell agglutination (RCA) test was evaluated for sensitivity in detecting and titering hepatitis B antigen (HB Ag) in comparison with two counterelectrophoresis (CEP) systems and a solid-phase radioimmunoassay (RIA). The RCA procedure was found to be significantly more sensitive than the CEP methods and compares favorably in sensitivity with the solid-phase RIA, detecting even lower concentrations of the HB Ag. Since the RCA test can be completed in 2 to 3 h and requires relatively inexpensive equipment, it offers a highly sensitive and rapid procedure suitable for use in blood banks to screen donors or detect low levels of antigen in serum of patients.
