RESUMEN
A method for the identification and quantification of hydroxyzine in human fluids by GC/NPD is presented. The method employs acepromazine as the internal standard and requires no derivatization. After a single alkaline extraction in n-heptane isoamylalcohol (98.5:1.5, v/v), analysis is achieved in 7 min. The lower limit of detectability was 0.8 ng/ml in plasma. Capillary gas chromatography mass spectrometry assay was developed for confirmation. Toxicological findings, after a fatality involving hydroxyzine are presented as an application of the procedure.
Asunto(s)
Hidroxizina/análisis , Suicidio , Adolescente , Cromatografía de Gases , Femenino , Cromatografía de Gases y Espectrometría de Masas , Humanos , Hidroxizina/sangre , Hidroxizina/orina , Reproducibilidad de los ResultadosRESUMEN
Toxicological analyses on a putrefied cadaver are sometimes difficult to achieve because of the absence of blood and urine. In this study, maggots, living material, are proposed as a new medium of investigation in forensic medicine. Five drugs (triazolam, oxazepam, phenobarbital, alimemazine, and clomipramine) were identified and assayed in some tissues of a putrefied cadaver and in the maggots found on and in the body.
Asunto(s)
Benzodiazepinas/análisis , Cadáver , Clomipramina/análisis , Dípteros/análisis , Fenobarbital/análisis , Trimeprazina/análisis , Animales , Humanos , Larva/análisis , Masculino , Persona de Mediana Edad , Oxazepam/análisis , Cambios Post Mortem , Triazolam/análisisRESUMEN
This article describes a selective gas chromatographic method for the resolution and quantification of phenoperidine and its two metabolites, pethidine (meperidine) and norpethidine (normeperidine). Drugs and SKF 525 A, the internal standard, are separated from plasma by solvent extraction under alkaline conditions. They are chromatographed on a 3% OV-17 Chromosorb Q glass column and detected with a nitrogen-phosphorous detector. Linearity is observed in the study range (5-200 ng/ml). No interference by endogenous substances is noted.
Asunto(s)
Inhibidores de la Colinesterasa/sangre , Meperidina/análogos & derivados , Meperidina/sangre , Fenoperidina/sangre , Inhibidores de la Colinesterasa/aislamiento & purificación , Cromatografía de Gases , Humanos , Meperidina/aislamiento & purificación , Fenoperidina/aislamiento & purificación , Valor Predictivo de las PruebasRESUMEN
A sensitive and specific assay for the simultaneous quantification of trihexyphenidyl and its hydroxylated metabolite in plasma and urine is described. The method is based on the extraction of the drugs with an organic solvent and separation on a 3% OV-17 Chromosorb Q column in a gas chromatograph equipped with a nitrogen-phosphorus detector. The procedure employs SKF 525 A as the internal standard and requires no derivatization. The detection limit was found to be 2 ng/mL for trihexyphenidyl and 1 ng/mL for its metabolite. The precision of the assay procedure for both compounds is about 4 to 7%.