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Pinworm infections are one of the common problems in laboratory rodents and man. At present there are only few drugs against intestinal helminths, and new drugs are urgently needed to cope up any future risk of drug resistance. Interest in plant secondary metabolites (PSMs) has risen considerably in the recent years for the discovery and development of new drugs. In the present study, we explored the in vitro and in vivo anthelmintic potentials of two pentacyclic triterpenoids, ursolic acid (UA) and betulinic acid (BA), the important PSMs of many medicinal plants, against Syphacia obvelata (Nematoda: Oxyuridae), a common pinworm of mice. The results of this study indicated that in both, in vitro and in vivo assays, BA showed comparatively better anthelmintic effects than UA. In the in vitro assay, 1.00 mg/ml concentration of BA showed paralysis and mortality of worms in 1.20 ± 0.04 and 2.30 ± 0.03 h, respectively. In the in vivo assay, a single 10.00 mg/kg dose of BA, administered for 5 days, revealed 68.78% reduction in egg counts and 84.08% reduction in worm counts of infected mice. The present study suggests that BA holds a great promise to be pursued further for detailed testing against some other representative group of helminth parasites.
RESUMEN
An inexpensive Ru(ii) complex catalyzes the oxidative annulation reaction of disubstituted alkynes with benzamidines to provide highly valuable 1-aminoisoquinolines in high yields. The reaction also features excellent regioselectivity with some unsymmetrical alkynes.
RESUMEN
PURPOSE: Functional bladder neck obstruction has been definitively diagnosed in the last few years due to detailed synchronous pressure flow, electromyography and video urodynamics. Clean intermittent self-catheterization and bladder neck incision are the modalities of treatment. To our knowledge the role of alpha-blockers is not yet defined in women. A new technique was developed to perform bladder neck incision using a pediatric resectoscope. MATERIALS AND METHODS: A total of 24 women with obstructive voiding symptoms or retention were evaluated with video pressure flow electromyography, and diagnosed with functional bladder neck obstruction due to high pressure and low flow on silent electromyography and bladder neck appearance on fluoroscopy. Patients were initially treated with clean intermittent self-catheterization and alpha-blockers. Catheterization was stopped when post-void residual was less than 50 ml. and only alpha-blocker therapy was continued. Bladder neck incision was performed in patients who had a poor response to or side effects of alpha-blocker therapy, or when therapy was discontinued due to economic reasons. Clean intermittent self-catheterization was continued in patients who had a poor response to alpha-blockers or refused to undergo bladder neck incision. Bladder neck incision was performed in the initial 2 cases with an adult resectoscope using a Collin's knife and subsequently a pediatric resectoscope (13F). Uroflow and post-void residual measurements were performed in all cases. RESULTS: Of the 24 patients 12 (50%) showed improvement in symptoms, peak flow and post-void residual (p <0.01) with alpha-blocker therapy only. Of the 12 patients who had a poor response to alpha-blockers 6 underwent bladder neck incision subsequently and 6 remained on clean intermittent self-catheterization. All 8 patients treated with bladder neck incision, including 2 who had a good response but discontinued alpha-blocker therapy, had sustained improvement in post-void residual and peak flow (p <0.01) after a mean followup of 3.8 +/- 2.4 years. Grade 1 stress incontinence in 2 adult resectoscope cases responded to conservative treatment. None of the pediatric resectoscope cases had stress incontinence. CONCLUSIONS: Clean intermittent self-catheterization and alpha-blockers are the initial treatment options for functional bladder neck obstruction. The alpha-blockers were successful in 50% of our patients. Bladder neck incision should be offered judiciously with minimal risk of curable stress incontinence. The pediatric resectoscope is useful to make a well controlled incision safely in the female urethra.
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Antagonistas Adrenérgicos alfa/uso terapéutico , Fenoxibenzamina/uso terapéutico , Prazosina/análogos & derivados , Prazosina/uso terapéutico , Proctoscopios , Obstrucción del Cuello de la Vejiga Urinaria/terapia , Adulto , Anciano , Terapia Combinada , Diseño de Equipo , Femenino , Humanos , Persona de Mediana Edad , Estudios RetrospectivosRESUMEN
Iodine content of rice (42) and drinking water (108) from a goitre-endemic belt of Assam, a sub-Himalayan zone of India, was evaluated. Iodine content of staple food (rice) and drinking water was found to be poor. Mean iodine content of rice was found to be 11.8 +/- 7.3 micrograms/100g. Lower level of iodine was also observed in drinking water samples (mean of 1.52 +/- 0.48 micrograms/l).
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Dieta , Bocio Endémico/etiología , Yodo/análisis , Oryza/química , Agua/química , Estudios de Evaluación como Asunto , Humanos , IndiaRESUMEN
Beginning 1991, a sudden rise of malaria cases were recorded in Tarajulie TE (Assam) coupled with mortality due to malaria. Deaths were confirmed due to Plasmodium falciparum (Pf) infections and were recorded in all age groups excluding infants. Malaria positives were recorded in all months of the year, however, there was a increased hospital attendance due to fever/malaria positives during May to September. During the years (1991-1993), the slide positive rate was as high as 33.04%, mostly being Pf infections (69%), and the annual parasite index ranged between 6 to 304 per thousand population. Morbidity and mortality due to malaria were attributed to labor movements to and fro from garden premises to adjoining hamlets, the latter being the site of acquisition of the infections.
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Agricultura , Malaria/epidemiología , Adolescente , Niño , Preescolar , Humanos , Incidencia , India/epidemiología , Lactante , Mortalidad , Estaciones del Año , TéRESUMEN
P. falciparum was the predominant parasite ( > 80%) species in malaria ridden tea estates of Assam. A simplified 3 day in vivo test to determine chloroquine sensitivity in tea garden tribes revealed that the majority of the cases (85%) were S/RI, 7% were RI, and 3% were RII and 5% were RIII, respectively. Early case detection and treatment were deemed necessary to reduce morbidity and mortality due to P. falciparum in these tea estates.
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Cloroquina/farmacología , Malaria Falciparum/tratamiento farmacológico , Plasmodium falciparum/efectos de los fármacos , Adolescente , Adulto , Anciano , Animales , Niño , Preescolar , Cloroquina/uso terapéutico , Resistencia a Medicamentos , Femenino , Humanos , India , Lactante , Malaria Falciparum/sangre , Malaria Falciparum/prevención & control , Masculino , Persona de Mediana Edad , Salud RuralRESUMEN
Twenty-five buffalo calves (age: 14 +/- 1.9 months; body weight: 102 +/- 9.4 kg) were subjected to intravenous regional analgesia (IRA) of the hind limb with 2% lignocaine HCl (4 mg/kg b.w.), infused through the dorsal digital vein. In control animals 15 ml of normal saline was infused. The effect of tourniquet release after 35 (8 calves) and 65 min (7 calves) on EEG, ECG, arterial and venous pressures, blood gases and pH, respiration and temperature were studied. A detectable concentration of lignocaine occurred before the release of tourniquet. The lignocaine concentration recorded in the systemic circulation did not produce any significant nervous or cardiovascular changes before or after release of tourniquet. Transient EEG and ECG changes were seen in six (tourniquet release after 35 min) and three (tourniquet release after 65 min) IRA animals, respectively. No significant changes were seen in heart rate, respiratory rate and rectal temperature, systolic, diastolic, mean arterial and central venous pressures. Arterial blood pH, PCO2 and HCO3- concentration showed no significant differences. Animals receiving IRA did not show hypoxaemia typical to postural changes.
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Analgesia/veterinaria , Anestesia de Conducción/veterinaria , Anestesia Intravenosa/veterinaria , Búfalos/fisiología , Miembro Posterior/fisiología , Animales , Electrocardiografía/veterinaria , Electroencefalografía/veterinaria , Lidocaína , MasculinoRESUMEN
A clinical survey of foot lameness in cattle indicated incidence of 9.2% (432/4695), with 365 cases (84.5%) occurring in hot humid zones having high rainfall. Of the 432 affected animals, 399 (92.4%) were females; most were Jerseys and Friesians 3-7 years old. Scissor claw (32.6%), overgrown hoof (27.2%) and corkscrew claw (18.2%) were the most common foot abnormalities. Other less frequently observed conditions included beak claw, bruising of sole, interdigital growth, claw hypoplasia and arthritis. More forefeet (238) than hindfeet (104) were affected.