RESUMEN
Assembly of the PSI (photosystem I) complex in eukaryotic photosynthetic organisms depends on the concerted interactions of the nuclear and chloroplast genetic systems. We have identified several nucleus-encoded factors of Chlamydomonas reinhardtii that are specifically required for the synthesis of the two large chloroplast-encoded reaction-centre polypeptides, PsaA and PsaB, of photosystem I and that function at plastid post-transcriptional steps. Raa1, Raa2 and Raa3 are required for the splicing of the three discontinuous psaA precursor transcripts; they are part of large RNA-protein complexes that are reminiscent of spliceosomal particles. Tab1 and Tab2 are involved in the initiation of translation of the psaB mRNA and are localized in the membrane and stromal phases of the chloroplast, where they are associated with high-molecular-mass complexes. Moreover, two chloroplast-encoded proteins, Ycf3 and Ycf4, are required for the primary steps of assembling the photosystem I subunits into a functional complex.
Asunto(s)
Chlamydomonas reinhardtii/fisiología , Complejo de Proteína del Fotosistema I/fisiología , Procesamiento Postranscripcional del ARN , Animales , Chlamydomonas reinhardtii/genética , Mutación , Complejo de Proteína del Fotosistema I/genética , Empalme del ARNRESUMEN
In Chlamydomonas reinhardtii, the psaA mRNA is assembled by a process involving trans-splicing of separate transcripts, encoded at three separate loci of the chloroplast genome. At least 14 nuclear loci and one chloroplast gene, tscA, are needed for this process. We have cloned Raa3, the first nuclear gene implicated in the splicing of intron 1. The predicted sequence of Raa3 consists of 1783 amino acids and shares a small region of homology with pyridoxamine 5'-phosphate oxidases. Raa3 is present in the soluble fraction of the chloroplast and is part of a large 1700 kDa complex, which also contains tscA RNA and the first psaA exon transcript. These partners, in association with other factors, form a chloroplast RNP particle that is required for the splicing of the first intron of psaA and which may be the counterpart of eukaryotic snRNPs involved in nuclear splicing.
Asunto(s)
Proteínas Algáceas/genética , Cloroplastos/metabolismo , Intrones , Complejos de Proteína Captadores de Luz , Proteínas Nucleares/metabolismo , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Complejo de Proteína del Fotosistema I , Proteínas de Plantas/genética , ARN de Planta , Trans-Empalme , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Chlamydomonas reinhardtii/genética , Chlamydomonas reinhardtii/metabolismo , Proteínas de Cloroplastos , Clonación Molecular , ADN de Plantas , Exones , Genes de Plantas , Datos de Secuencia Molecular , Proteínas Nucleares/genética , Proteínas Protozoarias/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , SolubilidadRESUMEN
Genetic analysis has revealed that the accumulation of several chloroplast mRNAs of the green alga Chlamydomonas reinhardtii requires specific nucleus-encoded functions. To gain insight into this process, we have cloned the nuclear gene encoding the Mbb1 factor by genomic rescue of a mutant specifically deficient in the accumulation of the mRNAs of the psbB/psbT/psbH chloroplast transcription unit. Mbb1 is a soluble protein in the stromal phase of the chloroplast. It consists of 662 amino acids with a putative chloroplast-transit peptide at its N-terminal end. A striking feature is the presence of 10 tandemly arranged tetratricopeptide-like repeats that account for half of the protein sequence and are thought to be involved in protein-protein interactions. The Mbb1 protein seems to have a homologue in higher plants and is part of a 300-kDa complex that is associated with RNA. This complex is most likely involved in psbB mRNA processing, stability, and/or translation.
Asunto(s)
Proteínas Algáceas , Chlamydomonas reinhardtii/genética , Complejos de Proteína Captadores de Luz , Familia de Multigenes , Proteínas Nucleares/metabolismo , Fosfoproteínas/genética , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Complejo de Proteína del Fotosistema II , Proteínas de Plantas/metabolismo , Proteínas de Unión al ARN/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Núcleo Celular/metabolismo , Chlamydomonas reinhardtii/metabolismo , Cloroplastos/metabolismo , Clonación Molecular , ADN de Plantas , Expresión Génica , Genes de Plantas , Datos de Secuencia Molecular , Proteínas Nucleares/genética , Proteínas de Plantas/genética , ARN de Planta/metabolismo , Proteínas de Unión al ARN/genética , Análisis de Secuencia de ADNRESUMEN
The expression of the plastid genome is dependent on a large number of nucleus-encoded factors. Some of these factors have been identified through biochemical assays, and many others by genetic screens in Arabidopsis, Chlamydomonas and maize. Nucleus-encoded factors function in each step in plastid gene expression, including transcription, RNA editing, RNA splicing, RNA processing, RNA degradation, and translation. Many of the factors discovered via biochemical approaches play general roles as components of the basic gene expression machinery, whereas the majority of those identified by genetic approaches are specifically required for the expression of small subsets of chloroplast genes and are involved in post-transcriptional steps. Some of the nucleus-encoded factors may play regulatory roles and modulate chloroplast gene expression in response to developmental or environmental cues. They may also serve to couple chloroplast gene expression with the assembly of the protein products into the large complexes of the photosynthetic apparatus. The convergence of biochemical approaches with those of classical and reverse genetics, and the contributions from large scale genomic sequencing should result in rapid advances in our understanding of the regulatory interactions that govern plastid gene expression.
Asunto(s)
Núcleo Celular/genética , Cloroplastos/genética , Regulación de la Expresión Génica de las Plantas , Plastidios/químicaRESUMEN
The chloroplast gene psbB encodes the chlorophyll-a binding protein P5 (CP47), one of the core subunits of photosystem II (PSII). The psbB mRNA and the downstream psbT and psbH transcripts fail to accumulate in the Chlamydomonas reinhardtii nuclear mutant 222E affected in the Mbb1 gene (Monod et al. 1992, Mol. Gen. Genet. 231, 449-459). By introducing chimeric genes consisting of sequences from psbB and the reporter gene aadA into the chloroplast, the target site of Mbb1 was mapped in the psbB 5' untranslated region (UTR). Primer extension analysis indicates that the psbB RNA exists in a less abundant long form and a more abundant short form, with 5' ends at positions -147 and -35 relative to the AUG initiation codon, respectively. The longer transcript is present both in the wild type (WT) and 222E mutant, but the shorter one accumulates only in the WT. Two putative stem-loop structures in the longer 5' UTR can be deleted individually without affecting psbB mRNA accumulation. Insertion of a poly G cassette in the long leader stabilizes a chimeric psbB transcript in the 222E mutant, suggesting the involvement of a 5'-3' exonuclease. We also show that psbH and psbT are transcribed from the upstream psbB gene promoter, and that the psbH mRNA has its own target sequence for Mbb1 function. We discuss the role of this nucleus-encoded factor, required for specific chloroplast gene expression, in the assembly of the multi-protein PSII complex.
Asunto(s)
Chlamydomonas reinhardtii/genética , Cloroplastos/metabolismo , Complejos de Proteína Captadores de Luz , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Complejo de Proteína del Fotosistema II , ARN Mensajero/genética , Regiones no Traducidas 5' , Animales , Secuencia de Bases , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Poli G/genética , Regiones Promotoras Genéticas , ARN Mensajero/química , Proteínas Recombinantes de Fusión/genética , Eliminación de SecuenciaRESUMEN
In Chlamydomonas reinhardtii, the psaA mRNA is assembled by a process involving two steps of trans-splicing that remove two group II introns and give rise to the mature mRNA. The products of at least 14 nuclear genes and one chloroplast gene (tscA) are necessary for this process. We have cloned Maa2, one of the nuclear genes involved in trans-splicing of the second intron. Maa2 encodes a protein with similarity to conserved domains of pseudouridine synthases, but mutagenesis of putative catalytic residues showed that this activity may not be required for trans-splicing of psaA RNA. Although it is not clear whether the pseudouridine synthase activity has been maintained in Maa2, it is possible that this enzyme was recruited during evolution as an RNA chaperone for folding or stabilizing the psaA intron. The Maa2 protein appears to be associated through ionic interactions with a low density membrane system in the chloroplast that also contains RNA-binding proteins involved in translation.
Asunto(s)
Proteínas Bacterianas/genética , Chlamydomonas reinhardtii/genética , Cloroplastos/genética , Hidroliasas/genética , Hidroliasas/metabolismo , Intrones , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Complejo de Proteína del Fotosistema I , Empalme del ARN , Transcripción Genética , Secuencia de Aminoácidos , Animales , Núcleo Celular/genética , Clonación Molecular , Exones , Hidroliasas/química , Proteínas de la Membrana/química , Datos de Secuencia Molecular , Proteínas Protozoarias , ARN Mensajero/genética , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de AminoácidoRESUMEN
Plastid proteins are encoded in two genomes, one in the nucleus and the other in the organelle. The expression of genes in these two compartments in coordinated during development and in response to environmental parameters such as light. Two converging approaches reveal features of this coordination: the biochemical analysis of proteins involved in gene expression, and the genetic analysis of mutants affected in plastid function or development. Because the majority of proteins implicated in plastid gene expression are encoded in the nucleus, regulatory processes in the nucleus and in the cytoplasm control plastid gene expression, in particular during development. Many nucleus-encoded factors involved in transcriptional and posttranscriptional steps of plastid gene expression have been characterized. We are also beginning to understand whether and how certain developmental or environmental signals perceived in one compartment may be transduced to the other.
Asunto(s)
Regulación de la Expresión Génica de las Plantas , Plantas/genética , Núcleo Celular/genética , Cloroplastos/genéticaRESUMEN
An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival.
Asunto(s)
Proteínas Algáceas , Chlamydomonas reinhardtii/genética , Cloroplastos/genética , Proteínas de la Membrana/genética , Sistemas de Lectura Abierta , Secuencia de Aminoácidos , Animales , Chlamydomonas reinhardtii/crecimiento & desarrollo , Proteínas de la Membrana/fisiología , Datos de Secuencia Molecular , Mutagénesis Insercional , Homología de Secuencia de AminoácidoRESUMEN
In the secondary structure model that has been proposed for the trans-spliced intron 1 in the Chlamydomonas reinhardtii psaA gene, a third RNA species (tscA RNA) interacts with the 5' and 3' intron parts flanking the exons to reconstitute a composite structure with several features of group-II introns. To test the validity of this model, we undertook the sequencing and modelling of equivalent introns in the psaA gene from other unicellular green algae belonging to the highly diversified genus Chlamydomonas. Our comparative analysis supports the model reported for the C. reinhardtii psaA intron 1, and also indicates that the 5' end of the tscA RNA and the region downstream from the psaA exon 1 cannot be folded into a structure typical of domain I as described for most group-II introns. It is possible that a fourth RNA species, yet to be discovered, provides the parts of domain I which are apparently missing.
Asunto(s)
Proteínas Bacterianas/genética , Chlamydomonas/genética , ADN de Cloroplastos/genética , Intrones , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Complejo de Proteína del Fotosistema I , Empalme del ARN/genética , Animales , Secuencia de Bases , Northern Blotting , Southern Blotting , Modelos Moleculares , Datos de Secuencia Molecular , Proteínas Protozoarias/genética , ARN Mensajero/química , ARN Mensajero/genética , ARN de Transferencia de Isoleucina/química , ARN de Transferencia de Isoleucina/genética , Alineación de Secuencia , Endonucleasas Específicas del ADN y ARN con un Solo Filamento/farmacologíaRESUMEN
We have engineered and analyzed a chloroplast mutant of Chlamydomonas reinhardtii that lacks ycf8, the chloroplast open reading frame 8, which is highly conserved in location and predicted amino acid sequence in land plants and C.reinhardtii. The ycf8 sequence was replaced with the aadA cassette which confers resistance to spectinomycin when expressed in the chloroplast. Although the mutant is able to grow phototrophically, photosystem II function and cell growth are impaired under stress conditions such as high light intensity and diminished chloroplast protein synthesis induced by spectinomycin. Use of an antibody generated against the ycf8 product has revealed that this hydrophobic polypeptide is associated with photosystem II, based on its severely reduced levels in various photosystem II-deficient mutants and on its copurification with photosystem II. This protein, therefore, appears to be (i) a novel photosystem II subunit and (ii) required for maintaining optimal photosystem II activity under adverse growth conditions.
Asunto(s)
Chlamydomonas reinhardtii/genética , Cloroplastos/fisiología , Complejos de Proteína Captadores de Luz , Fotosíntesis/genética , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Complejo de Proteína del Fotosistema II , Animales , División Celular , Chlamydomonas reinhardtii/efectos de los fármacos , Chlamydomonas reinhardtii/fisiología , Chlamydomonas reinhardtii/efectos de la radiación , Ingeniería Genética , Membranas Intracelulares/fisiología , Luz , Proteínas de la Membrana/genética , Proteínas de la Membrana/fisiología , Sistemas de Lectura Abierta/genética , Fotosíntesis/fisiología , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Eliminación de Secuencia , Espectinomicina/farmacologíaRESUMEN
Using particle gun-mediated chloroplast transformation we have disrupted the psbK gene of Chlamydomonas reinhardtii with an aadA expression cassette that confers resistance to spectinomycin. The transformants are unable to grow photoautotrophically, but they grow normally in acetate-containing medium. They are deficient in photosystem II activity as measured by fluorescence transients and O2 evolution and they accumulate less than 10% of wild-type levels of photosystem II as measured by immunochemical means. Pulse-labeling experiments indicate that the photosystem II complex is synthesized normally in the transformants. These results differ from those obtained previously with similar cyanobacterial psbK mutants that were still capable of photoautotrophic growth (Ikeuchi et al., J. Biol. Chem. 266 (1991) 1111-1115). In C. reinhardtii the psbK product is required for the stable assembly and/or stability of the photosystem II complex and essential for photoautotrophic growth. The data also suggest that the stability requirements of the photosynthetic complexes differ considerably between C. reinhardtii and cyanobacteria.
Asunto(s)
Proteínas Algáceas , Chlamydomonas reinhardtii/metabolismo , Fotosíntesis/fisiología , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Complejo de Proteína del Fotosistema II , Proteínas de Plantas/genética , Proteínas Protozoarias , Animales , Chlamydomonas reinhardtii/genética , Chlamydomonas reinhardtii/crecimiento & desarrollo , Cloroplastos/fisiología , Sustancias Macromoleculares , Mutagénesis , Transformación GenéticaRESUMEN
A chloroplast-encoded gene, designated chlB, has been isolated from Chlamydomonas reinhardtii, its nucleotide sequence determined, and its role in the light-independent reduction of protochlorophyllide to chlorophyllide demonstrated by gene disruption experiments. The C. reinhardtii chlB gene is similar to open reading frame 563 (orf563) of C. moewusii, and its encoded protein is a homolog of the Rhodobacter capsulatus bchB gene product that encodes one of the polypeptide components of bacterial light-independent protochlorophyllide reduction. To determine whether the chlB gene product has a similar role in light-independent protochlorophyllide reduction in this alga, a series of plasmids were constructed in which the aadA gene conferring spectinomycin resistance was inserted at three different sites within the chlB gene. The mutated chlB genes were introduced into the Chlamydomonas chloroplast genome using particle gun-mediated transformation, and homoplasmic transformants containing the disrupted chlB genes were selected on the basis of conversion to antibiotic resistance. Individual transformed strains containing chlB disruptions were grown in the dark or light, and 17 of the 18 strains examined were found to have a "yellow-in-the-dark" phenotype and to accumulate the chlorophyll biosynthetic precursor protochlorophyllide. RNA gel blot analysis of chlB gene expression in wild-type cells indicated that the gene was transcribed at low levels in both dark- and light-grown cells. The results of these studies support the involvement of the chlB gene product in light-independent protochlorophyllide reduction, and they demonstrate that, similar to its eubacterial predecessors, this green alga requires at least three components (i.e., chlN, chlL, and chlB) for light-independent protochlorophyllide reduction.
Asunto(s)
Chlamydomonas reinhardtii/enzimología , Chlamydomonas reinhardtii/genética , Genes Protozoarios , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH , Oxidorreductasas/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Chlamydomonas reinhardtii/efectos de la radiación , Clorofila/biosíntesis , Clorofila/genética , Cloroplastos/enzimología , Mapeo Cromosómico , ADN Protozoario/genética , Expresión Génica , Luz , Datos de Secuencia Molecular , Mutagénesis Insercional , Oxidorreductasas/metabolismo , Fenotipo , Pigmentación/genética , Homología de Secuencia de AminoácidoRESUMEN
We have isolated and characterized a nuclear mutant, 222E, in Chlamydomonas reinhardtii, which is defective in photosystem II (PSII). Polypeptide P5, the product of psbB, is not produced in this mutant, leading to a destabilization of other PSII components. The mutant specifically fails to accumulate psbB transcripts and displays an altered transcription pattern downstream of psbB. Pulse-labelling experiments suggest that mRNA stability and/or processing are affected by the alteration of a nuclear gene product in this mutant. We show that the C. reinhardtii psbB gene is co-transcribed with a small open reading frame that is highly conserved in location and amino acid sequence in land plants. The 5' and 3' termini of the psbB transcript have been mapped to 35 bases upstream of the initiation codon and approximately 600 bases downstream of the stop codon. The 3' flanking region contains two potential stem-loops, of which the larger (with an estimated free energy of -46 kcal) is near the 3' terminus of the transcript.
Asunto(s)
Núcleo Celular/fisiología , Chlamydomonas reinhardtii/genética , Cloroplastos/metabolismo , ADN/genética , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , ARN Mensajero/metabolismo , Secuencia de Aminoácidos , Animales , Composición de Base , Secuencia de Bases , Northern Blotting , Chlamydomonas reinhardtii/metabolismo , Clonación Molecular , ADN/aislamiento & purificación , Datos de Secuencia Molecular , Mutación , Conformación de Ácido Nucleico , Oligodesoxirribonucleótidos , Fotosíntesis/genética , Proteínas del Complejo del Centro de Reacción Fotosintética/biosíntesis , Complejo de Proteína del Fotosistema II , ARN/genética , ARN/aislamiento & purificación , ARN Mensajero/genética , Mapeo Restrictivo , Homología de Secuencia de Ácido NucleicoRESUMEN
Expression vectors for Chlamydomonas reinhardtii chloroplast transformation have been constructed with transcription and translation signals from chloroplast genes. The bacterial aadA sequence, coding for aminoglycoside 3" adenyl transferase, was inserted in these vectors and introduced into the C. reinhardtii chloroplast by particle gun transformation. The stable transgenic expression of this foreign protein in the chloroplast confers spectinomycin and streptomycin resistance to the transformed cells. This new marker can be used as a reporter of gene expression, and as a portable selectable cassette for chloroplast reverse genetics. Targetted gene disruption mutants of loci required for photosynthesis, tscA and psaC, were thus obtained. A gene disruption of an unidentified open reading frame, ORF472, remained heteroplasmic, suggesting that it has a vital function.
Asunto(s)
Chlamydomonas/genética , Cloroplastos/metabolismo , Nucleotidiltransferasas/genética , Transformación Genética/genética , Secuencia de Bases , Northern Blotting , Southern Blotting , Expresión Génica/fisiología , Marcadores Genéticos , Vectores Genéticos/genética , Intrones/genética , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida/genética , Proteínas Recombinantes de Fusión/biosíntesisRESUMEN
The chloroplast gene psaC encoding the iron sulfur protein of photosystem I (PSI) from the green alga Chlamydomonas reinhardtii has been cloned and characterized. The deduced amino acid sequence is highly related to that of higher plants and cyanobacteria. Using a particle gun, wild type C. reinhardtii cells have been transformed with a plasmid carrying the psaC gene disrupted by an aadA gene cassette designed to express spectinomycin/streptomycin resistance in the chloroplast. Transformants selected on plates containing acetate as a reduced carbon source and spectinomycin are unable to grow on minimal medium lacking acetate and are deficient in PSI activity. Southern blot analysis of total cell DNA of the transformants shows that the wild type psaC gene has been replaced by the interrupted psaC gene through homologous recombination. While authentic transcripts of the psaC gene are no longer detected, aadA gives rise to a few transcripts in the transformants. Biochemical analysis indicates that neither PSI reaction center subunits nor the seven small subunits belonging to PSI accumulate stably in the thylakoid membranes of the transformants. Pulse-chase labeling of cell proteins shows that the PSI reaction center subunits are synthesized normally but turn over rapidly in the transformants. We conclude that the iron sulfur binding protein encoded by the psaC gene is an essential component, both for photochemical activity and for stable assembly of PSI. The present study suggests that any chloroplast gene encoding a component of the photosynthetic apparatus can be disrupted in C. reinhardtii using the strategy described.
Asunto(s)
Chlamydomonas/genética , Cloroplastos , Elementos Transponibles de ADN , Proteínas Hierro-Azufre/genética , Proteínas de la Membrana , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Complejo de Proteína del Fotosistema I , Proteínas/genética , Secuencia de Aminoácidos , Secuencia de Bases , Northern Blotting , Southern Blotting , ADN/genética , Electroforesis en Gel de Poliacrilamida , Genes , Cinética , Datos de Secuencia Molecular , Fenotipo , ARN/genética , Homología de Secuencia de Ácido Nucleico , Espectrometría de Fluorescencia , Transformación GenéticaRESUMEN
In C. reinhardtii, the mature psaA mRNA is assembled by a process involving trans-splicing of three separate transcripts encoded at three widely scattered loci of the chloroplast genome. At least one additional chloroplast locus (tscA) is required for trans-splicing of exons 1 and 2. We have mapped this gene by transformation of a deletion mutant with a particle gun. The 0.7 kb region of the chloroplast genome that is sufficient to rescue tscA function has been subjected to insertion mutagenesis, showing that it does not contain significant open reading frames. We suggest from these experiments that the product of the tscA gene may be a small chloroplast RNA that acts in trans in the first trans-splicing reaction of psaA. A model for the mode of action of this RNA is presented, in which the characteristic structure of group II introns is assembled from three separate transcripts.
Asunto(s)
Chlamydomonas/genética , Cloroplastos/metabolismo , Empalme del ARN , ARN/genética , Secuencia de Aminoácidos , Secuencia de Bases , ADN , Intrones , Modelos Genéticos , Datos de Secuencia Molecular , Mutagénesis Insercional , Conformación de Ácido Nucleico , Mapeo Restrictivo , Transcripción Genética , Transformación GenéticaRESUMEN
In Chlamydomonas reinhardtii the three exons of the psaA gene are widely scattered on the chloroplast genome: exons 1 and 2 are in opposite orientations and distant from each other and from exon 3. The mature mRNA, encoding a core polypeptide of photosystem I, is thus probably assembled from separate precursors by splicing in trans. We have isolated and characterized a set of mutants that are deficient in the maturation of psaA mRNA. The mutants belong to 14 nuclear complementation groups and one chloroplast locus that are required for the assembly of psaA mRNA. The chloroplast locus, tscA, is remote from any of the exons and must encode a factor required in trans. The mutants all show one of only three phenotypes that correspond to defects in one or other or both of the joining reactions. These phenotypes, and those of double mutants, are consistent with the existence of two alternative splicing pathways.
Asunto(s)
Chlamydomonas/genética , Mutación , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Empalme del ARN , Cloroplastos/metabolismo , Exones , Prueba de Complementación Genética , Intrones , Fenotipo , Complejo de Proteína del Fotosistema I , Procesamiento Postranscripcional del ARN , Recombinación Genética , Mapeo RestrictivoRESUMEN
The nuclear NAC2 locus of C. reinhardtii encodes a trans-acting factor that confers stability on the chloroplast message for the D2 protein of photosystem II (PS II). A mutant that carries a lesion in the NAC2 gene is unable to accumulate the psbD transcript encoding D2, but synthesizes all other PS II proteins normally. However, the PS II complex is completely unstable in the nac2-26 mutant, and all major PS II polypeptides, including the three oxygen-evolving enhancing proteins, are absent or greatly reduced in this strain because of posttranslational degradation. In C. reinhardtii the second exon of the trans-spliced psaA mRNA is cotranscribed with psbD (Choquet et al., 1988), but the nac2-26 mutation normally has no effect on psaA message maturation or stability. However, in double mutants carrying the nac2-26 mutation, as well as a mutation that prevents psaA splicing, splicing intermediates carrying psbD sequences are degraded. These results suggest that the NAC2 gene product acts in a very specific manner to control the half-life of psbD transcripts.
Asunto(s)
Chlamydomonas/genética , Clorofila/genética , Cloroplastos/fisiología , Proteínas de Plantas/genética , Regulación de la Expresión Génica , Genes , Complejos de Proteína Captadores de Luz , Mutación , Proteínas del Complejo del Centro de Reacción Fotosintética , Complejo de Proteína del Fotosistema II , Procesamiento Postranscripcional del ARN , ARN Mensajero/metabolismo , Transcripción GenéticaRESUMEN
To study the import of polypeptide precursors we have adapted and compared two procedures for the isolation of competent chloroplasts from the green unicellular alga, Chlamydomonas reinhardtii: silicasol gradient centrifugation and elutriation. The chloroplasts actively import the precursor of the small subunit of ribulose bisphosphate carboxylase-oxygenase in vitro.
RESUMEN
We have isolated complementary DNA (cDNA) clones for apocytochrome c from the green alga Chlamydomonas reinhardtii and shown that they are encoded by a single nuclear gene termed cyc. Cyc mRNA levels are found to depend primarily on the presence of acetate as a reduced carbon source in the culture medium. The deduced amino acid sequence shows that, apart from the probable removal of the initiating methionine, C. reinhardtii apocytochrome c is synthesized in its mature form. Its structure is generally similar to that of cytochromes c from higher plants. Several punctual deviations from the general pattern of cytochrome c sequences that is found in other organisms have interesting structural and functional implications. These include, in particular, valines 19 and 39, asparagine 78, and alanine 83. A phylogenetic tree was constructed by the matrix method from cytochrome c data for a representative range of species. The results suggest that C. reinhardtii diverged from higher plants approximately 700-750 million years ago; they also are not easy to reconcile with the current attribution of Chlamydomonas reinhardtii and Enteromorpha intestinalis to a unique phylum, because these two species probably diverged from one another at about the same time as they diverged from the line leading to higher plants.