RESUMEN
A comparative analysis of age-related dynamics of spermatogenesis has been performed in mutant mouse lines predisposed or resistant to accelerated senescence (SAMP1 and SAMR1 respectively). The results show that quantitative and morphohistological trends in the development of sperm cells and Sertoli cells in both lines are similar in both lines. Their comparison with data obtained in our previous studies (Zakhidov et al., 2001; Gordeeva et al., 2001) shows that sharp quantitative and qualitative changes in the structure of the spermatogenic system have occurred in senescence-accelerated mice of new generations, which confirms the fact of dynamic instability of the germinal lineage. The role of stem spermatogonial cells in restoration of spermatogenesis in animals reaching the critical age is discussed.
Asunto(s)
Envejecimiento/patología , Espermatogénesis , Testículo/patología , Envejecimiento/fisiología , Animales , Peso Corporal/fisiología , Masculino , Ratones , Ratones Endogámicos , Modelos Animales , Tamaño de los Órganos/fisiología , Epitelio Seminífero/patología , Epitelio Seminífero/fisiología , Células de Sertoli/patología , Células de Sertoli/fisiología , Recuento de Espermatozoides , Espermatozoides/patología , Espermatozoides/fisiología , Testículo/fisiologíaRESUMEN
Carnosine significantly increased the number of spermatogonia and Sertoli cells in mice prone (SAMP1) and resistant (SAMR1) to accelerated aging and appreciably reduced cell yield in meiosis and spermiogenesis in SAMP1 mice. In experimental SAMP1 mice catastrophic changes in the number of gametes were paralleled by intensive degradation of the spermatogenic epithelium. In SAMR1 mice treated with carnosine highly ordered spermatogenic structure was preserved.
Asunto(s)
Carnosina/farmacología , Células Epiteliales/metabolismo , Espermatogénesis/efectos de los fármacos , Espermatozoides/efectos de los fármacos , Envejecimiento , Animales , Senescencia Celular , Masculino , Meiosis , Ratones , Células de Sertoli/patología , Espermatozoides/patología , Testículo/metabolismo , Factores de TiempoAsunto(s)
Envejecimiento/genética , Inestabilidad Genómica/genética , Espermatozoides/metabolismo , Envejecimiento Prematuro/genética , Animales , Cromatina/metabolismo , ADN/análisis , Interpretación Estadística de Datos , Modelos Animales de Enfermedad , Mutación de Línea Germinal/genética , Histocitoquímica , Masculino , Ratones , Cabeza del Espermatozoide/metabolismo , Espermátides/química , Espermátides/citología , Espermatogénesis/genética , Espermatozoides/química , Espermatozoides/crecimiento & desarrollo , Testículo/citología , Testículo/metabolismoRESUMEN
The quantitative micronucleus test showed that the natural dipeptide carnosine increases the count of aberrant spermatogonia and round spermatids in the testes in SAMR1 mice resistant to accelerated aging by 64 and 85%, respectively, compared to the control. However, this agent did not modify the incidence of chromosome mutations in spermatogenic cells in SAMP1 mice predisposed to accelerated aging.
Asunto(s)
Envejecimiento , Carnosina/farmacología , Senescencia Celular , Testículo/efectos de los fármacos , Animales , Carnosina/metabolismo , Masculino , Ratones , Ratones Endogámicos , Pruebas de Micronúcleos , Espermátides/efectos de los fármacos , Espermatogonias/efectos de los fármacosRESUMEN
It was shown that during ontogenesis, the mice prone to (SAMP1) and resistant against accelerates senescence did not differ substantially in the frequency of cytogenetic aberrations in the hepatocytes and spermatogenic cells (spermatozoa and circular spermatids). These data suggest that in the mice of both lines, the processes of appearance, development, and functioning of complex biological systems, such as liver and testis, take place against the background of high genetic instability. The role of genetic instability in senescence is discussed.
