RESUMEN
The accumulation of viral proteins p24 and gp41 in MT-4 cells was shown to occur asynchronously. No increase in the number of polydiploid cells, no polyploid mitoses or chromosome defects were found indicating the antiproliferative effect of HIV on MT-4 cells. A cyclic pattern of the infection course was observed in infected Jurkat-tat cells. The accumulation of viral proteins was concerted, their maximum amounts preceding the cell death. HIV-1 did not inhibit cell division and caused strong disorders in the chromosome structure.
Asunto(s)
Aberraciones Cromosómicas , Infecciones por VIH/microbiología , VIH-1/patogenicidad , Linfocitos T/microbiología , Enfermedad Aguda , Línea Celular , Células Cultivadas/metabolismo , Células Cultivadas/microbiología , Efecto Citopatogénico Viral , Citofotometría , Proteína p24 del Núcleo del VIH/metabolismo , Proteína gp41 de Envoltorio del VIH/metabolismo , Infecciones por VIH/genética , Infecciones por VIH/metabolismo , Humanos , Inmunohistoquímica , Poliploidía , Linfocitos T/metabolismo , Factores de TiempoRESUMEN
Mutant clones of hepatocarcinoma cell line PLC-PRF-5 containing integrated hepatitis B virus genome were derived by multistep selection for resistance to some toxic agents. These clones were found to display various stages of cell differentiation according to growth rate, ability to synthesize alpha-fetoprotein, to grow in semisolid medium and to cloning in agar. In the majority of the clones with higher stages of differentiation the expression of HBsAg gene was demonstrated to be increased as compared to the original line, and in some of these clones the expression of silent HBcAg was activated.
Asunto(s)
Carcinoma Hepatocelular/genética , Regulación Viral de la Expresión Génica/genética , Virus de la Hepatitis B/genética , Neoplasias Hepáticas/genética , Animales , Carcinoma Hepatocelular/inmunología , Transformación Celular Neoplásica/genética , Transformación Celular Neoplásica/inmunología , Resistencia a Medicamentos/genética , Resistencia a Medicamentos/inmunología , Regulación Viral de la Expresión Génica/inmunología , Genoma Viral , Antígenos del Núcleo de la Hepatitis B/genética , Antígenos del Núcleo de la Hepatitis B/inmunología , Antígenos de Superficie de la Hepatitis B/genética , Antígenos de Superficie de la Hepatitis B/inmunología , Virus de la Hepatitis B/inmunología , Humanos , Neoplasias Hepáticas/inmunología , Ratones , Ratones Desnudos , Trasplante de Neoplasias , Selección Genética , Células Tumorales Cultivadas/inmunologíaRESUMEN
A complex karyological analysis of three human cell lines (PLC-PRF-5, MT-4 and U937) was carried out that involved traditional methods of G-, C-banding and silver staining, in addition to the in situ hybridization technique using 4 alpha-satellite DNA probes: DNA specific for centromeric regions of chromosome 11, 6, 13, and 21, and 14 and 22. The application of this additional method allowed to identify, prove or detalize the structure of 13 markers in PLC-PRF-5 cells, 1 marker in MT-4 cells, and 3 markers in U937 cells. The results show that the in situ hybridization method would be successful in cell line karyotyping for a more objective identification of some markers difficult for analysis.
Asunto(s)
Aberraciones Cromosómicas , ADN de Neoplasias/genética , ADN/genética , Hibridación in Situ/métodos , Carcinoma Hepatocelular/ultraestructura , Línea Celular , Bandeo Cromosómico/métodos , Sondas de ADN , VIH-1 , Humanos , Cariotipificación , Neoplasias Hepáticas/ultraestructura , Células Tumorales Cultivadas , Cultivo de VirusRESUMEN
Silver staining and acrocentric chromosome association (ACA) patterns were investigated in bone marrow cells as well as in peripheral blood cells (cultures with or without PHA) from 39 patients with chronic myelocytic leukemia (CML), including 20 cases being in blastic phase (BP CML), and 51 patients with acute leukemia (AL). Bone marrow cells and PHA-stimulated peripheral blood lymphocytes (from 10 and 17 healthy donors, respectively) were used as a control. The frequency of ACA in metaphases from bone marrow cells of all the above groups of patients was shown to be decreased compared to that in PHA-stimulated lymphocytes. Patients with BP CML and AL constituted the most heterogeneous groups although some of them demonstrated the highest ACA-frequency per cell. There is a pronounced correlation between Ag+-nucleolus organizer regions (NOR's) and the frequency of ACA. With the exception of CML, the correlation coefficients (0.83, 0.74, and 0.72) were highly significant for all the above groups (donors, BP CML, and AL patients, respectively). The distribution pattern of single chromosome pairs, according to their ACA frequency, differed with every individual studied, but it was similar in normal and leukemic cells of the same individual. From the above data a conclusion is made that the frequency of ACA may depend on the functional activity of the NOR's as well as on the cells type.
