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1.
Pathologe ; 25(3): 209-16, 2004 May.
Artículo en Alemán | MEDLINE | ID: mdl-15138702

RESUMEN

The Papanicolaou smear (Pap) is a worldwide screening tool for early detection of cervical cancer and its precursor lesions. The transition from dysplasia to cancer is a highly complex genetic process and cannot be predicted based solely on cell morphology. Molecular characterization of precursor lesions could yield a better definition of lesions at high risk for progression. We developed an analytical concept comprising not only morphological characterization but also molecular analysis with multiple parameters of dysplastic cells from cervical smears. We isolated dysplastic cells from 52 fixed Pap-stained smears of various grades by laser microdissection and analyzed them for genetic lesions typical for cervical carcinoma. The loss of heterozygosity (LOH) as published for cervical carcinoma tissue was detected. Markers for early stages showed a LOH in 43% and 22%, and those for late stages in 26% of the cases. Combining morphological characterization with molecular analysis by multiple molecular markers could open up new opportunities for early detection of cervical carcinoma.


Asunto(s)
Neoplasias del Cuello Uterino/patología , Femenino , Marcadores Genéticos , Humanos , Rayos Láser , Pérdida de Heterocigocidad , Microdisección/instrumentación , Microdisección/métodos , Prueba de Papanicolaou , Neoplasias del Cuello Uterino/genética , Frotis Vaginal
2.
Histochemistry ; 102(5): 353-64, 1994 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-7532638

RESUMEN

In this study, we wished to clarify the distribution and co-localization of nitric oxide synthase and NA-DPH-diaphorase (NADPH-d) in nerve cells, nerve fibres and parenchymal cells in exocrine and endocrine pancreas, and to assess the influence of fixation on the staining pattern obtained. For this purpose, we applied nitric oxide synthase immunocytochemistry and NADPH-d histochemistry to rat and human pancreas under different fixation conditions. Antibodies to neuronal and endothelial nitric oxide synthase were similarly applied. We found complete co-localization of neuronal nitric oxide synthase and NADPH-d in ganglion cells, and in nerve fibres around acini, excretory ducts, blood vessels and in islets of Langerhans of rat and human pancreas. Immunoreactivity for endothelial nitric oxide synthase was co-localized with NADPH-d in endothelial cells. However, in NADPH-d reactive islet and ductal epithelial cells we could detect neither brain nor endothelial nitric oxide synthase immunoreactivity with any fixation protocol applied. There were marked differences in NADPH-d staining of both neurons and parenchymal cells under different fixation conditions. These results indicate the existence of different types of NADPH-d, which are associated or not associated with nitric oxide synthase(s), and which are differently influenced by various fixation procedures in rat and human pancreas.


Asunto(s)
Aminoácido Oxidorreductasas/análisis , NADPH Deshidrogenasa/análisis , Neuronas/enzimología , Páncreas/enzimología , Fijación del Tejido/métodos , Acetona , Aminoácido Oxidorreductasas/inmunología , Secuencia de Aminoácidos , Animales , Capsaicina/farmacología , Crioultramicrotomía , Endotelio/enzimología , Femenino , Formaldehído , Ganglios/química , Ganglios/citología , Glutaral , Histocitoquímica , Humanos , Inmunohistoquímica , Islotes Pancreáticos/enzimología , Masculino , Datos de Secuencia Molecular , Óxido Nítrico Sintasa , Embarazo , Efectos Tardíos de la Exposición Prenatal , Ratas , Ratas Sprague-Dawley , Ratas Wistar , Coloración y Etiquetado/métodos
3.
Histopathology ; 24(2): 169-71, 1994 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-8181811

RESUMEN

Ectopic mammary tissue in the inguinal region giving rise to a hamartoma is reported. This localization of ectopic breast is infrequent and hamartomas of the breast at this site have not been described previously. The unusual size of the lesion caused pre-operative diagnostic difficulties.


Asunto(s)
Mama , Coristoma/patología , Ingle/patología , Hamartoma/patología , Mama/anomalías , Femenino , Humanos , Persona de Mediana Edad
4.
Langenbecks Arch Chir ; 378(5): 281-7, 1993.
Artículo en Alemán | MEDLINE | ID: mdl-8412436

RESUMEN

Before 1978, where cystic tumors of the pancreas were concerned, pathologists only differentiated between cystic adenomas and cystadenocarcinomas. Recently, however, further tumor entities have been introduced. We now differentiate between the generally benign serous cystic adenoma, the potentially malignant mucinous cystadenoma, the possibly malignant papillary cystic tumor, and the always malignant mucinous cystadenocarcinoma. Other rare tumors include the solid cystic acinous-cell tumor, the cystic islet tumor, and mucinous ductal hyperplasia. Because of their slow growth and primary displacement nature, all of these tumors can usually be detected only after they have attained considerable size. Computed tomography (CT), sonography and endoscopic retrograde cholangiopancreatography (ERCP) have an established role in diagnosis. With these methods, as a rule, it is possible to identify pseudocysts; however, differentiation between the individual tumor types is almost impossible. In our study from 1979 to 1990, we observed ten cases of serous cystic adenomas, nine cystadenocarcinomas, and four malignant papillary-cystic tumors. Of these, nine of the ten serous cystic adenomas, four of the five mucinous cystadenomas, all four papillary-cystic tumors, and five of the nine cystadenocarcinomas were curatively resected. All patients with curatively resected adenomas and one patient with an R1-resected cystic adenoma remained free of recurrence throughout the follow-up period. One 86-year-old female patient in whom a serous cystic adenoma was histologically determined still has no symptoms 8 years after diagnosis despite slow tumor progression. Two of the five patients in whom a cystadenocarcinoma was curatively resected died postoperatively; a third patient died of tumor recurrence 4 months following resection.(ABSTRACT TRUNCATED AT 250 WORDS)


Asunto(s)
Cistadenocarcinoma/cirugía , Cistoadenoma/cirugía , Neoplasias Pancreáticas/cirugía , Adulto , Anciano , Anciano de 80 o más Años , Causas de Muerte , Cistadenocarcinoma/mortalidad , Cistoadenoma/mortalidad , Cistoadenoma/patología , Diagnóstico por Imagen , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Páncreas/patología , Pancreatectomía , Neoplasias Pancreáticas/mortalidad , Neoplasias Pancreáticas/patología , Tasa de Supervivencia
5.
Z Orthop Ihre Grenzgeb ; 128(2): 223-6, 1990.
Artículo en Alemán | MEDLINE | ID: mdl-2140658

RESUMEN

The effect of sodium chloride solution on disc tissue was investigated in vitro. Tissue material was obtained from 19 discectomies. The specimens were incubated in aqua ad injectionem and in different sodium chloride solutions and weighted regularly during a maximum of 24 hours. Following the incubation periods the specimens were examined histologically. After one hour the surgical specimens showed a weight increase of 290% for aqua bidest., 170% for 0.9% NaCl and 100% for 5.85% NaCl and 10% NaCl. Disc tissue obtained from six autopsies was also examined under the same conditions. The weight increase in nucleus pulposus and anulus fibrosus tissue was less than in the disc tissue obtained from surgery. The weight increase in anulus fibrosus tissue was less than in nucleus pulposus tissue. In vitro incubation of disc tissue in a hypermolar sodium chloride solution always resulted in a weight increase. Successful therapy could not be explained by shrinkage of disc tissue treated with hypermolar sodium chloride.


Asunto(s)
Desplazamiento del Disco Intervertebral/terapia , Disco Intervertebral/efectos de los fármacos , Vértebras Lumbares/efectos de los fármacos , Solución Salina Hipertónica/administración & dosificación , Adulto , Técnicas de Cultivo , Relación Dosis-Respuesta a Droga , Humanos , Inyecciones Epidurales , Desplazamiento del Disco Intervertebral/cirugía , Vértebras Lumbares/cirugía , Microcirugia
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