[Experimental microbiological research on instrument and denture disinfection with a disinfectant spray for dental practice and the use of patients. 1. The formulation of the problem and bacteriological studies]. / Mikrobiologisch-experimentelle Untersuchungen zur Instrumenten- und Prothesendesinfektion mit Desinfektionsmittel-Spray für die stomatologische Praxis und zu Händen des Patienten. 1. Mitteilung: Problemstellung, bakteriologische Untersuchungen.

Three disinfectant sprays (Arugeen, Desident and Fesia-sept) proved to be very efficient against pure cultures of aero bacteria species, Cand. albicans, saliva flora and entero- and influenza viruses. The methods included carrier experiments using denture basis resins, fragments of orthodontic appliances and screws (instead of instruments).

[Bacteriological findings in bronchial secretions of children with chronic, nontuberculous lung diseases and their relationship to local transferrin values]. / Bakteriologische Befunde bei Bronchialsekreten von Kindern mit chronischen, nichttuberkulösen Lungenerkrankungen sowie ihre Beziehungen zum lokalen Transferrin-Gehalt.

In 142 bronchial secretions from 63 children with chronic nontuberculous lung diseases the content of transferrin was studied. In 68 samples (= 48%) transferrin has been found in concentrations between 0.1 and 13.9 mg/100 mg total protein. Fifty six of the secretions were analysed bacteriologically, microbes have been isolated in 37 of them (66%). Of the detected bacteria 5 species were considered as potentially pathogenic. The bacterial growth has been shown more frequently in secretions of patients with heavily inflamed bronchial mucosa. In bacterially contaminated secretions the occurrence of transferrin was reduced.

[Experiences in the preparation of the secretory IgA and secretory component from human colostrum]. / Erfahrungen bei der Präparation von sekretorischem IgA und sekretorischer Komponente aus Humankolostrum.

With the isolation of secretory IgA and secretory component from human colostrum following the route communicated by Kobayashi some differences revealed with respect to the preparative results. Mainly problems arising from lactoferrin and IgM encouraged us to offer some varied laboratory instructions.

[Testing the disinfectant effect of calcium peroxide as a root canal filling material]. / Prüfung der desinfizierenden Wirkung des Kalziumperoxids als Wurzelkanalfüllmaterial.

The authors have been using with success calcium peroxide as a root-canal filling material for many years. Its bactericidal and bacteriostatic effects are tested. Germ carrier tests illustrate the suitability of its pasty preparation for root-canal disinfection.

[Bacteriological findings in materials from patients with nonspecific odontogenic infections]. / Erregerbefunde im Untersuchungsmaterial von Patienten mit unspezifischen odontogenen Infektionen

The results of bacteriological studies made between 1964 and 1971 by workers at the Varia Laboratory, Institute of Medical Microbiology on 767 patients treated at the Clinic of Gnathofacial Surgery, Department of Medicine, Friedrich Schiller University at Jena, are evaluated. The testees (x=275) were included in groups with specific diagnoses, and a breakdown was made according to the detection of aerobic pus-forming germs. Gram-positive cocci were detected in 92% of the cases and their pathogenetic representatives, 76,5% of the cases. Rodshaped germs (Enterobacteriaceae, Pseudomonas) were observed only in 37,9% of the cases. Staphylococcus aureus, which accounted for 62,5%, was the most frequently observed species. It was followed, in order of frequency of detection, by greening streptococci and Staphylococcus epidermidis. Monoinfection was far more frequently observed than multi-infection. Also, the problems associated with the differentiation of streptococci are pointed out since other investigators found streptococci to be the most frequent agents producing odontogenic infections.

[Resistance to chemotherapy of aerobic bacteria from nonspecific odontogenic infections]. / Zur chemotherapeutischen Resistenz aerober bakterieller Erreger aus unspezifischen odontogenen Infektionen

Germs in patients suffering from nonspecific odontogenous infections described in a previous paper were analysed with regard to their resistance to chemotherapy (1964 to 1971). At the same time it was discovered that in the bacteria groups: staphyloccocus aureus, streptococci and pneumococci and enterobacteriaceae plus pseudomonas (344 strains) their sensitivity to penicillin, streptomycin, chloramphenicol, oxytetracyclin, erythromycin, polymyxin B and nifurantin varied in several and significant ways. Significant changes were established in the resistance of staphylococcus aureus to all the potentially effective antibiotics, while from 1969 to 1971 the strains with the least resistance appeared. In the 1970 to 1971 period strepto- and pneumococci showed insensitivity to penicillin and streptomycin more frequently than before. From 1969 to 1971 entero-bacteriaceae and pseudomonas also showed evidence of increasing loss of sensitivity to chloramphenicol and oxytetracyclin, whereas the frequency of strains resistant to polymyxin B and nifurantine diminished. The findings are being discussed.

[Bacteriological studies during conservative treatment of periapical inflammations]. / Bakteriologische Untersuchungen während der konservierenden Behandlung periapikaler Entzündungen

32 teeth with the diagnosis of periapical osteitis (established by clinical and radiological examinations) were subjected to endodontic treatment (irrigation with hydrogen peroxide), the course of which was followed by bacteriological examinations. The timing of treatment termination depended solely upon clinical evaluation. The examination of smears taken before the last irrigation showed that 26 of the 32 root canals (81%) were germ-free; in 2 cases, an anaerobic infection was still extant; and in 4 cases, an aerobic infection was observed. The high percentage of root canals which were germ-free before the last irrigation testifies to the efficiency of hydrogen peroxide application for disinfecting root canals on the one hand, and demonstrates the good agreement between clinical and bacteriological findings on the other hand. The germs identified belonged mainly to the normal oral flora. Enterococci proved to be particularly resistant. The conservative treatment of periapical osteitis by means of root-canal disinfection with a hydrogen peroxide preparation ans subsequent root-canal filling with a calcium peroxide paste is recommended.