Anthropogenic noise and light pollution decrease the repeatability of activity patterns and dampen expression of chronotypes in a free-living songbird.

Anthropogenic environmental change is introducing a suite of novel disturbance factors, which can have wide-ranging effects on mean behavior and behavioral repeatability. For example, exposure to sensory pollutants, such as anthropogenic noise and artificial light at night (ALAN), may affect consistent and repeatable individual-level timing of daily activity, which is referred to as chronotypes. Although chronotypes have been increasingly documented in wild animal populations and may affect fitness, evidence for long-term stability across life-history stages and seasons is notably lacking. Furthermore, how multiple anthropogenic stressors may interact to erode or magnify the expression of chronotypes remains unclear. We tested for existence of chronotypes across life-history stages and seasons in suburban female great tits (Parus major), using emergence time from nest boxes in the morning as a proxy for activity onset. We then examined joint effects of noise pollution and ALAN on expression of chronotypes, and tested for effects of noise, ALAN, and weather conditions on mean emergence time. We found repeatability of daily activity patterns (emergence times) across life-history stages and seasons, providing evidence of chronotypes, as well as interactive effects of anthropogenic disturbance factors and weather conditions on population mean behavior. Furthermore, across-season repeatability of emergence times was approximately double in magnitude in low light and low noise conditions, relative to in conditions with higher light and/or noise pollution. Thus, joint exposure to these sensory pollutants tends to erode expression of chronotypes. This effect was driven by higher among-individual variance in the relatively undisturbed environment and collapse of this variance in the more disturbed environments. Decreased repeatability in environments with high disturbance levels may reduce potential for behavioral traits, such as chronotype, to be the target of selection and limit adaptability.

Cognition as a neglected mediator of responses to anthropogenic noise.

Anthropogenic noise is an increasingly pervasive global disturbance factor, with diverse biological effects. Yet, most studies have focused on population mean responses to noise pollution, leaving sources of among-individual differences in responses poorly understood. Blackburn et al. (2023) provide the first evidence from free-living animals that cognition might mediate individual differences in responses to noise pollution. In this commentary, we highlight the contribution of this ground-breaking study to stimulate more research on this important topic. We argue that cognition might mediate among-individual differences in the ability to cope with both masking effects and stress associated with noise pollution.

Keystone seabird may face thermoregulatory challenges in a warming Arctic.

Climate change affects the Arctic more than any other region, resulting in evolving weather, vanishing sea ice and altered biochemical cycling, which may increase biotic exposure to chemical pollution. We tested thermoregulatory impacts of these changes on the most abundant Arctic seabird, the little auk (Alle alle). This small diving species uses sea ice-habitats for foraging on zooplankton and resting. We equipped eight little auks with 3D accelerometers to monitor behavior, and ingested temperature recorders to measure body temperature (Tb). We also recorded weather conditions, and collected blood to assess mercury (Hg) contamination. There were nonlinear relationships between time engaged in different behaviors and Tb. Tb increased on sea ice, following declines while foraging in polar waters, but changed little when birds were resting on water. Tb also increased when birds were flying, and decreased at the colony after being elevated during flight. Weather conditions, but not Hg contamination, also affected Tb. However, given our small sample size, further research regarding thermoregulatory effects of Hg is warranted. Results suggest that little auk Tb varies with behavior and weather conditions, and that loss of sea ice due to global warming may cause thermoregulatory and energic challenges during foraging trips at sea.

Combined threats of climate change and contaminant exposure through the lens of bioenergetics.

Organisms face energetic challenges of climate change in combination with suites of natural and anthropogenic stressors. In particular, chemical contaminant exposure has neurotoxic, endocrine-disrupting, and behavioral effects which may additively or interactively combine with challenges associated with climate change. We used a literature review across animal taxa and contaminant classes, but focused on Arctic endotherms and contaminants important in Arctic ecosystems, to demonstrate potential for interactive effects across five bioenergetic domains: (1) energy supply, (2) energy demand, (3) energy storage, (4) energy allocation tradeoffs, and (5) energy management strategies; and involving four climate change-sensitive environmental stressors: changes in resource availability, temperature, predation risk, and parasitism. Identified examples included relatively equal numbers of synergistic and antagonistic interactions. Synergies are often suggested to be particularly problematic, since they magnify biological effects. However, we emphasize that antagonistic effects on bioenergetic traits can be equally problematic, since they can reflect dampening of beneficial responses and result in negative synergistic effects on fitness. Our review also highlights that empirical demonstrations remain limited, especially in endotherms. Elucidating the nature of climate change-by-contaminant interactive effects on bioenergetic traits will build toward determining overall outcomes for energy balance and fitness. Progressing to determine critical species, life stages, and target areas in which transformative effects arise will aid in forecasting broad-scale bioenergetic outcomes under global change scenarios.

Endocrine effects of exposure to artificial light at night: A review and synthesis of knowledge gaps.

Animals have evolved with natural patterns of light and darkness, such that light serves as an important zeitgeber, allowing adaptive synchronization of behavior and physiology to external conditions. Exposure to artificial light at night (ALAN) interferes with this process, resulting in dysregulation of endocrine systems. In this review, we evaluate the endocrine effects of ALAN exposure in birds and reptiles, identify major knowledge gaps, and highlight areas for future research. There is strong evidence for ecologically relevant levels of ALAN acting as an environmental endocrine disruptor. However, most studies focus on the pineal hormone melatonin, corticosterone release via the hypothalamus-pituitary-adrenal axis, or regulation of reproductive hormones via the hypothalamus-pituitary-gonadal axis, leaving effects on other endocrine systems largely unknown. We call for more research spanning a diversity of hormonal systems and levels of endocrine regulation (e.g. circulating hormone levels, receptor numbers, strength of negative feedback), and investigating involvement of molecular mechanisms, such as clock genes, in hormonal responses. In addition, longer-term studies are needed to elucidate potentially distinct effects arising from chronic exposure. Other important areas for future research effort include investigating intraspecific and interspecific variability in sensitivity to light exposure, further distinguishing between distinct effects of different types of light sources, and assessing impacts of ALAN exposure early in life, when endocrine systems remain sensitive to developmental programming. The effects of ALAN on endocrine systems are likely to have a plethora of downstream effects, with implications for individual fitness, population persistence, and community dynamics, especially within urban and suburban environments.

Contaminant-by-environment interactive effects on animal behavior in the context of global change: Evidence from avian behavioral ecotoxicology.

The potential for chemical contaminant exposure to interact with other stressors to affect animal behavioral responses to environmental variability is of mounting concern in the context of anthropogenic environmental change. We systematically reviewed the avian literature to evaluate evidence for contaminant-by-environment interactive effects on animal behavior, as birds are prominent models in behavioral ecotoxicology and global change research. We found that only 17 of 156 (10.9 %) avian behavioral ecotoxicological studies have explored contaminant-by-environment interactions. However, 13 (76.5 %) have found evidence for interactive effects, suggesting that contaminant-by-environment interactive effects on behavior are understudied but important. We draw on our review to develop a conceptual framework to understand such interactive effects from a behavioral reaction norm perspective. Our framework highlights four patterns in reaction norm shapes that can underlie contaminant-by-environment interactive effects on behavior, termed exacerbation, inhibition, mitigation and convergence. First, contamination can render individuals unable to maintain critical behaviors across gradients in additional stressors, exacerbating behavioral change (reaction norms steeper) and generating synergy. Second, contamination can inhibit behavioral adjustment to other stressors, antagonizing behavioral plasticity (reaction norms shallower). Third, a second stressor can mitigate (antagonize) toxicological effects of contamination, causing steeper reaction norms in highly contaminated individuals, with improvement of performance upon exposure to additional stress. Fourth, contamination can limit behavioral plasticity in response to permissive conditions, such that performance of more and less contaminated individuals converges under more stressful conditions. Diverse mechanisms might underlie such shape differences in reaction norms, including combined effects of contaminants and other stressors on endocrinology, energy balance, sensory systems, and physiological and cognitive limits. To encourage more research, we outline how the types of contaminant-by-environment interactive effects proposed in our framework might operate across multiple behavioral domains. We conclude by leveraging our review and framework to suggest priorities for future research.

A keystone avian predator faces elevated energy expenditure in a warming Arctic.

Climate change is transforming bioenergetic landscapes, challenging behavioral and physiological coping mechanisms. A critical question involves whether animals can adjust behavioral patterns and energy expenditure to stabilize fitness given reconfiguration of resource bases, or whether limits to plasticity ultimately compromise energy balance. In the Arctic, rapidly warming temperatures are transforming food webs, making Arctic organisms strong models for understanding biological implications of climate change-related environmental variability. We examined plasticity in the daily energy expenditure (DEE) of an Arctic seabird, the little auk (Alle alle) in response to variability in climate change-sensitive drivers of resource availability, sea surface temperature (SST) and sea ice coverage (SIC), and tested the hypothesis that energetic ceilings and exposure to mercury, an important neurotoxin and endocrine disrupter in marine ecosystems, may limit scope for plasticity. To estimate DEE, we used accelerometer data obtained across years from two colonies exposed to distinct environmental conditions (Ukaleqarteq [UK], East Greenland; Hornsund [HS], Svalbard). We proceeded to model future changes in SST to predict energetic impacts. At UK, high flight costs linked to low SIC and high SST drove DEE from below to above 4 × basal metabolic rate (BMR), a proposed energetic threshold for breeding birds. However, DEE remained below 7 × BMR, an alternative threshold, and did not plateau. Birds at HS experienced higher, relatively invariable SST, and operated above 4 × BMR. Mercury exposure was unrelated to DEE, and fitness remained stable. Thus, plasticity in DEE currently buffers fitness, providing resiliency against climate change. Nevertheless, modeling suggests that continued warming of SST may promote accelerating increases in DEE, which may become unsustainable.

Mercury Contamination Challenges the Behavioral Response of a Keystone Species to Arctic Climate Change.

Combined effects of multiple, climate change-associated stressors are of mounting concern, especially in Arctic ecosystems. Elevated mercury (Hg) exposure in Arctic animals could affect behavioral responses to changes in foraging landscapes caused by climate change, generating interactive effects on behavior and population resilience. We investigated this hypothesis in little auks (Alle alle), a keystone Arctic seabird. We compiled behavioral data for 44 birds across 5 years using accelerometers while also quantifying blood Hg and environmental conditions. Warm sea surface temperature (SST) and low sea ice coverage reshaped time activity budgets (TABs) and diving patterns, causing decreased resting, increased flight, and longer dives. Mercury contamination was not associated with TABs. However, highly contaminated birds lengthened interdive breaks when making long dives, suggesting Hg-induced physiological limitations. As dive durations increased with warm SST, subtle toxicological effects threaten to increasingly constrain diving and foraging efficiency as climate change progresses, with ecosystem-wide repercussions.

Anthropogenic noise and light pollution additively affect sleep behaviour in free-living birds in sex- and season-dependent fashions.

Rapid anthropogenic transformation of environments exposes organisms to diverse disturbance factors, including anthropogenic noise pollution and artificial light at night (ALAN). These sensory pollutants interfere with acquisition of, and response to, environmental cues and can be perceived as stressors. Noise pollution and ALAN are often experienced simultaneously, and are thus likely to jointly affect organisms, either additively or interactively. Yet, combined effects of noise pollution and ALAN remain poorly elucidated. We studied combined effects of noise pollution and ALAN on the sleep behaviour of a free-living songbird, the great tit (Parus major). Sleep is widely conserved across animal taxa and fulfils essential functions, and research has demonstrated independent effects of both noise and ALAN on sleep. We measured noise and light levels at nest boxes and used infrared video-recording to assess sleep behaviour. Results did not support interactive effects of noise and ALAN. However, noise pollution and ALAN were both independently related to variation in sleep behaviour, in sex- and season-dependent fashions. Males, but not females, woke up and left the nest box ∼20 min later in the noisiest as compared to quietest environments (range: 44.2-79.4 dB), perhaps because males are more sensitive to acoustical cues that are masked by noise. Furthermore, as the season progressed from November to early March, birds woke up and left the nest box ∼35 min earlier relative to sunrise on territories with the lowest, but not the highest, light levels (range: 0.01-8.5 lux). Thus, the seasonal difference in sleep duration was dampened on light polluted territories. These effects could arise if ALAN interferes with birds' ability to sense and respond to increasing daylength, and could have fitness ramifications. Our study suggests that noise pollution and ALAN exert additive effects on sleep behaviour, and that these effects can be sex- and season-dependent.

An Experimental Approach to Investigating Effects of Artificial Light at Night on Free-Ranging Animals: Implementation, Results, and Directions for Future Research.

Animals have evolved with natural patterns of light and darkness. However, artificial light is being increasingly introduced into the environment from human infrastructure and recreational activity. Artificial light at night (ALAN) has the potential to have widespread effects on animal behavior, physiology, and fitness, which can translate into broader-scale effects on populations and communities. Understanding the effects of ALAN on free-ranging animals is non-trivial due to challenges such as measuring levels of light encountered by mobile organisms and separating the effects of ALAN from those of other anthropogenic disturbance factors. Here we describe an approach that allows us to isolate the effects of artificial light exposure on individual animals by experimentally manipulating light levels inside nest boxes. To this end, a system can be used consisting of light-emitting diode (LED) light(s) adhered to a plate and connected to a battery and timer system. The setup allows exposure of individuals inside nest boxes to varying intensities and durations of ALAN while simultaneously obtaining video recordings, which also include audio. The system has been used in studies on free-ranging great tits (Parus major) and blue tits (Cyanistes caeruleus) to gain insight into how ALAN affects sleep and activity patterns in adults and physiology and telomere dynamics in developing nestlings. The system, or an adaptation thereof, could be used to answer many other intriguing research questions, such as how ALAN interacts with other disturbance factors and affects bioenergetic balance. Furthermore, similar systems could be installed in or near the nest boxes, nests or burrows of a variety of species to manipulate levels of ALAN, evaluate biological responses, and work towards building an interspecific perspective. Especially when combined with other advanced approaches for monitoring the behavior and movement of free-living animals, this approach promises to yield ongoing contributions to our understanding of the biological implications of ALAN.

Serotonin transporter (SERT) polymorphisms, personality and problem-solving in urban great tits.

Understanding underlying genetic variation can elucidate how diversity in behavioral phenotypes evolves and is maintained. Genes in the serotonergic signaling pathway, including the serotonin transporter gene (SERT), are candidates for affecting animal personality, cognition and fitness. In a model species, the great tit (Parus major), we reevaluated previous findings suggesting relationships between SERT polymorphisms, neophobia, exploratory behavior and fitness parameters, and performed a first test of the relationship between single nucleotide polymorphisms (SNPs) in SERT and problem-solving in birds. We found some evidence for associations between SERT SNPs and neophobia, exploratory behavior and laying date. Furthermore, several SNPs were associated with behavioral patterns and success rates during obstacle removal problem-solving tests performed at nest boxes. In females, minor allele homozygotes (AA) for nonsynonymous SNP226 in exon 1 made fewer incorrect attempts and were more likely to problem-solve. In both sexes, there was some evidence that minor allele homozygotes (CC) for SNP84 in exon 9 were more likely to problem-solve. Only one SNP-behavior relationship was statistically significant after correcting for multiple comparisons, but several were associated with substantial effect sizes. Our study provides a foundation for future research on the genetic basis of behavioral and cognitive variation in wild animal populations.

Variable and consistent traffic noise negatively affect the sleep behavior of a free-living songbird.

Anthropogenic noise is a ubiquitous disturbance factor, which, owing to the extensive nature of transportation networks, and ability of sound waves to penetrate distances, has wide-reaching impacts on biological communities. Research effort on biological effects of anthropogenic noise is extensive, but has focused on waking behavior, and to our knowledge, no published experimental study exists on how noise affects sleep in free-living animals. Sleep plays vital functions in processes such as cellular repair and memory consolidation. Thus, understanding the potential for noise to disrupt sleep is a critical research objective. Whether different noise regimes exert distinct effects on behavior also remains poorly understood, as does intraspecific variation in noise sensitivity. To address these knowledge gaps, we used a repeated-measures field experiment involving broad-casting traffic noise recordings at great tit (Parus major) nest boxes over a series of consecutive nights. We evaluated whether increasing the temporal variability and amplitude of traffic noise increased deleterious effects on sleep behavior in free-living great tits, and whether individuals differed in the magnitude of responses. We found that traffic noise reduced sleep duration, proportion, and bout length, and induced birds to exit nest boxes earlier in the morning. There was some support for a stronger effect of more variable noise, and relative to lower amplitude noise, higher amplitude noise resulted in less and more fragmented sleep. Effects of noise on sleep duration were stronger in older adults, and substantial, repeatable variation existed in individual responses. We demonstrate for the first time that anthropogenic noise can have strong effects on sleep in free-living animals, which may have cascading effects on waking behavior, physiology and fitness. Results suggest that reducing the amplitude of traffic noise may be an effective mitigation strategy, and that differences in individual sensitivity are important to consider when evaluating effects of noise exposure. CAPSULE: Experimental exposure to temporally variable and consistent traffic noise negatively affected sleep behavior in a free-living songbird.

The serotonin transporter gene and female personality variation in a free-living passerine.

Quantifying variation in behaviour-related genes provides insight into the evolutionary potential of repeatable among-individual variation in behaviour (i.e. personality). Yet, individuals typically also plastically adjust their behaviour in response to environmental conditions and/or age, thereby complicating the detection of genotype-phenotype associations. Here, using a population of free-living great tits (Parus major), we assessed the association between single nucleotide polymorphisms (SNPs) in the serotonin transporter gene (SERT) and two repeatable behavioural traits, i.e. female-female aggression and female hissing behaviour. For female-female aggression, a trait showing age-related plasticity, we found no evidence for associations with SERT SNPs, even when assessing potential age-dependent effects of SERT genotype on aggression. We also found no strong support for associations between SERT SNPs and hissing behaviour, yet we identified two synonymous polymorphisms (exon 13 SNP66 and exon 12 SNP144) of particular interest, each explaining about 1.3% of the total variation in hissing behaviour. Overall, our results contribute to the general understanding of the biological underpinning of complex behavioural traits and will facilitate further (meta-analytic) research on behaviour-related genes. Moreover, we emphasize that future molecular genetic studies should consider age-dependent genotype-phenotype associations for behavioural trait (co)variation, as this will vastly improve our understanding of the proximate causes and ultimate consequences of personality variation in natural populations.

Little parental response to anthropogenic noise in an urban songbird, but evidence for individual differences in sensitivity.

Anthropogenic noise exposure has well-documented behavioral, physiological and fitness effects on organisms. However, whether different noise regimes evoke distinct responses has rarely been investigated, despite implications for tailoring noise mitigation policies. Urban animals might display low responsiveness to certain anthropogenic noise regimes, especially consistent noise (e.g. freeway noise), but might remain more sensitive to more diverse noise regimes. Additionally, whether individuals differ in noise sensitivity is a rarely explored issue, which is important to fully understand organismal responses to noise. To address these knowledge gaps, we used a field experiment to measure how urban great tits (Parus major) altered parental behaviors in response to two noise regimes: consistent freeway noise, and a diverse anthropogenic noise regime that incorporated variability in noise type and temporal occurrence. We also evaluated whether sex, age, or a well-described personality trait, novel environment exploration behavior, were associated with responses to noise, although our power to assess individual differences in responses was somewhat limited. We found no evidence for mean population-level changes in nestling provisioning behaviors during either noise treatment. However, despite this overall canalization of behavior, there was evidence for individual differences in noise sensitivity, particularly during the diverse noise treatment. Females and birds that explored a novel environment more rapidly (fast explorers) reduced nestling provisioning rate more relative to baseline levels than males and slow explorers during the diverse urban noise, but not during the consistent freeway noise. Furthermore, first year breeders and fast explorers displayed larger increases in latency to return to the nest box relative to baseline conditions during the diverse noise only. Results suggest that urban animal populations might become overall tolerant to anthropogenic noise, but that certain individuals within these populations nonetheless remain sensitive to certain types of noise exposure. CAPSULE: In an urban songbird, we found no population-level changes in nestling provisioning behavior during noise exposure, but did find evidence for individual differences in noise sensitivity.

Sources of individual variation in problem-solving performance in urban great tits (Parus major): Exploring effects of metal pollution, urban disturbance and personality.

Despite growing research effort, we have a limited understanding of how urban disturbance factors affect cognitive traits, such as innovative problem-solving. We performed an initial assessment of how metal pollution and urbanization levels on territories are related to problem-solving performance in urban great tits (Parus major), by presenting an obstacle removal test at nest boxes in three urban nest box populations that are exposed to different levels of metal pollution. We predicted that problem-solving capacity might be reduced within the most polluted population due to pollution-related neurological impairments. On the other hand, we predicted that problem-solving might positively correlate with urban disturbance levels on territories, because some past research suggests that problem-solving promotes persistence in urbanized habitats. We also assessed relationships between exploratory personality type, behavioral patterns during tests, and problem-solving performance, and examined the repeatability and fitness correlates of problem-solving. We found no evidence that behavioral patterns or problem-solving performance were related to metal exposure or exploratory personality, or that problem-solving promotes reproductive success. However, birds on territories exposed to more urban disturbance, as quantified by proximity to paths and roads, were more likely to problem-solve. Moreover, an aggressive problem-solving approach negatively predicted problem-solving success, and behavioral patterns during tests and problem-solving success were repeatable. Thus, rather than indicating negative effects of pollution or urban disturbance on problem-solving performance, our study provides preliminary support for the hypothesis that urbanization favors innovative problem-solving, and suggests that problem-solving could be associated with a personality dimension independent of exploratory personality.

Anthropogenic noise is associated with telomere length and carotenoid-based coloration in free-living nestling songbirds.

Growing evidence suggests that anthropogenic noise has deleterious effects on the behavior and physiology of free-living animals. These effects may be particularly pronounced early in life, when developmental trajectories are sensitive to stressors, yet studies investigating developmental effects of noise exposure in free-living populations remain scarce. To elucidate the effects of noise exposure during development, we examined whether noise exposure is associated with shorter telomeres, duller carotenoid-based coloration and reduced body mass in nestlings of a common urban bird, the great tit (Parus major). We also assessed how the noise environment is related to reproductive success. We obtained long-term measurements of the noise environment, over a ∼24-h period, and characterized both the amplitude (measured by LAeq, LA90, LA10, LAmax) and variance in noise levels, since more stochastic, as well as louder, noise regimes might be more likely to induce stress. In our urban population, noise levels varied substantially, with louder, but less variable, noise characteristic of areas adjacent to a highway. Noise levels were also highly repeatable, suggesting that individuals experience consistent differences in noise exposure. The amplitude of noise near nest boxes was associated with shorter telomeres among smaller, but not larger, brood members. In addition, carotenoid chroma and hue were positively associated with variance in average and maximum noise levels, and average reflectance was negatively associated with variance in background noise. Independent of noise, hue was positively related to telomere length. Nestling mass and reproductive success were unaffected by noise exposure. Results indicate that multiple dimensions of the noise environment, or factors associated with the noise environment, could affect the phenotype of developing organisms, that noise exposure, or correlated variables, might have the strongest effects on sensitive groups of individuals, and that carotenoid hue could serve as a signal of early-life telomere length.

Early-life exposure to artificial light at night elevates physiological stress in free-living songbirds☆.

Artificial light at night (ALAN) can disrupt adaptive patterns of physiology and behavior that promote high fitness, resulting in physiological stress and elevation of steroid glucocorticoids (corticosterone, CORT in birds). Elevated CORT may have particularly profound effects early in life, with the potential for enduring effects that persist into adulthood. Research on the consequences of early-life exposure to ALAN remains limited, especially outside of the laboratory, and whether light exposure affects CORT concentrations in wild nestling birds particularly remains to be elucidated. We used an experimental setup to test the hypothesis that ALAN elevates CORT concentrations in developing free-living birds, by exposing nestling great tits (Parus major) to ALAN inside nest boxes. We measured CORT in feathers grown over the timeframe of the experiment (7 nights), such that CORT concentrations represent an integrative metric of hormone release over the period of nocturnal light exposure, and of development. We also assessed the relationships between feather CORT concentrations, body condition, nestling size rank and fledging success. In addition, we evaluated the relationship between feather CORT concentrations and telomere length. Nestlings exposed to ALAN had higher feather CORT concentrations than control nestlings, and nestlings in poorer body condition and smaller brood members also had higher CORT. On the other hand, telomere length, fledging success, and recruitment rate were not significantly associated with light exposure or feather CORT concentrations. Results indicate that exposure to ALAN elevates CORT concentrations in nestlings, which may reflect physiological stress. In addition, the organizational effects of CORT are known to be substantial. Thus, despite the lack of an effect on telomere length and survivorship, elevated CORT concentrations in nestlings exposed to ALAN may have subsequent impacts on later-life fitness and stress sensitivity.

Carotenoid- but not melanin-based plumage coloration is negatively related to metal exposure and proximity to the road in an urban songbird.

Rapid urbanization is a global phenomenon that is increasingly exposing organisms to novel stressors. These novel stressors can affect diverse aspects of organismal function, including development of condition-dependent ornaments, which play critical roles in social and sexual selection. We investigated the relationship between metal pollution, proximity to roads, and carotenoid- and melanin-based plumage coloration in a common songbird, the great tit (Parus major). We studied populations located across a well-characterized metal pollution gradient and surrounded by roadway networks. Metal exposure and road-associated pollution could reduce carotenoid-based pigmentation by inducing oxidative stress or affecting habitat quality, but metals could also enhance melanin-based pigmentation, through effects on melanogenesis and testosterone concentrations. Using a large sample size (N > 500), we found that birds residing close to a point source for metals had reduced ultraviolet chroma, a component of carotenoid-based pigmentation. Moreover, birds with high feather metal concentrations had lower carotenoid chroma, hue, and ultraviolet chroma, with effects modified by age class. Birds residing closer to roads also had lower carotenoid chroma and hue. Melanin-based pigmentation showed high between-year repeatability, and no association with anthropogenic pollution. Results suggest that carotenoid-, but not melanin-, based pigmentation is negatively affected by multiple anthropogenic stressors. We are the first to demonstrate a negative association between roads and a plumage-based signaling trait, which could have important implications for sexual signaling dynamics in urban landscapes.

An Important Personality Trait Varies with Blood and Plumage Metal Concentrations in a Free-Living Songbird.

Metal pollution is a global problem, which threatens to seriously disrupt behavioral patterns and health in humans and wildlife. Nonetheless, little is known regarding how exposure to metal pollution affects animal personalities, as defined by repeatable among individual differences in behavior. We used a large dataset to investigate the relationship between individual blood and feather metal concentrations and three personality traits (exploration behavior, territorial aggressiveness, and aggressiveness during nest defense) in great tits (Parus major), a model species for animal personality research. We previously demonstrated slower exploration behavior at highly polluted study sites, where exposure to lead, cadmium, and arsenic is high. Here, we demonstrate the across-year repeatability of exploration behavior and aggressiveness during nest defense, providing strong evidence for the existence of personalities in our populations. Furthermore, we demonstrate that individuals with high blood lead concentrations and high concentrations of multiple metals in the feathers exhibit slower exploration behavior but no differences in territorial aggressiveness or nest defense relative to less exposed birds. The mechanism underlying the relationship between metal exposure and exploration behavior remains to be determined but could involve neurotoxic effects. Our study highlights that metal pollution could have underappreciated effects on animal personalities, with implications for individual fitness and societal function.