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AIMS: To (i) compare the limits of detection of Bacillus anthracis spores in three soils (one Florida, one Texas, and one a commercial Garden product) by PCR using DNA extracted with five commercial extraction kits and (ii) examine if removing organic acids or adding an enrichment step utilizing a growth medium will improve the detection limits. METHODS AND RESULTS: Bacillus anthracis spores were added to soil aliquots and used immediately with a DNA extraction kit or pretreated to remove organics or incubated overnight in a selective growth medium before the DNA extraction was performed. Using hybridization and PCR assays for capC, pag and lef genes, 10(5) -10(6) B. anthracis spores were detected in untreated Florida soil, 10(4) -10(7) spores in untreated Texas soil and 10(6) -10(7) in Garden soil. Pretreatment did not reliably improve detection. DNA from untreated and pretreated soils was suitable for hybridization but not always for PCR. When 10(1) -10(2) spores were added to the soils and allowed to amplify in a growth medium selective for B. anthracis, DNA extracted using four methods reliably produced PCR acceptable DNA positive for the B. anthracis genes. CONCLUSIONS: The quality of DNA extracted with commercial kits appears to be influenced by the soil type and pretreatment. Yet, with an enrichment step added, four of five extraction methods produced PCR suitable DNA and detected ≤10(2) spores. SIGNIFICANCE AND IMPACT OF THE STUDY: The enrichment step could enhance the detection of B. anthracis spores in soils and small samples contaminated with soil.
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Bacillus anthracis/genética , Técnicas Bacteriológicas/métodos , ADN Bacteriano/análisis , Microbiología del Suelo , Bacillus anthracis/crecimiento & desarrollo , Bacillus anthracis/aislamiento & purificación , Medios de Cultivo , Límite de Detección , Reacción en Cadena de la Polimerasa , Suelo/química , Esporas BacterianasRESUMEN
In this study, we compared mechanical factors in the reverse and three-inch power punches. Twelve expert male martial artists stood on a force plate, and executed reverse and power punches against a padded target fixed to a wall-mounted force plate. The force plates measured horizontal forces, and subsequently impulses and body centre of mass velocity changes. The motions of four markers attached to the arm were also collected, and were used to compute the horizontal velocities of the knuckle and of the arm centre of mass. The power punch produced smaller velocities immediately before impact than the reverse punch for the whole-body centre of mass (0.14 vs. 0.31 m x s(-1)), for the arm centre of mass (2.86 vs. 4.68 m x s(-1)), and for the knuckle (4.09 vs. 6.43 m x s(-1)). The peak force exerted by the fist was much smaller in the power punch than in the reverse punch (790 vs. 1450 N). However, the linear impulse exerted by the fist during the first 0.20 s of contact was slightly larger in the power punch than in the reverse punch (43.2 vs. 37.7 N x s). The results indicate that the power punch is less potent than the reverse punch, but slightly more effective for throwing the opponent off balance.
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Aceleración , Ergometría/instrumentación , Artes Marciales/fisiología , Fenómenos Biomecánicos , Humanos , Masculino , Estados UnidosRESUMEN
Methanotrophic bacteria play a major role in the global carbon cycle, degrade xenobiotic pollutants, and have the potential for a variety of biotechnological applications. To facilitate ecological studies of these important organisms, we developed a suite of oligonucleotide probes for quantitative analysis of methanotroph-specific 16S rRNA from environmental samples. Two probes target methanotrophs in the family Methylocystaceae (type II methanotrophs) as a group. No oligonucleotide signatures that distinguish between the two genera in this family, Methylocystis and Methylosinus, were identified. Two other probes target, as a single group, a majority of the known methanotrophs belonging to the family Methylococcaceae (type I/X methanotrophs). The remaining probes target members of individual genera of the Methylococcaceae, including Methylobacter, Methylomonas, Methylomicrobium, Methylococcus, and Methylocaldum. One of the family-level probes also covers all methanotrophic endosymbionts of marine mollusks for which 16S rRNA sequences have been published. The two known species of the newly described genus Methylosarcina gen. nov. are covered by a probe that otherwise targets only members of the closely related genus Methylomicrobium. None of the probes covers strains of the newly proposed genera Methylocella and "Methylothermus," which are polyphyletic with respect to the recognized methanotrophic families. Empirically determined midpoint dissociation temperatures were 49 to 57 degrees C for all probes. In dot blot screening against RNA from positive- and negative-control strains, the probes were specific to their intended targets. The broad coverage and high degree of specificity of this new suite of probes will provide more detailed, quantitative information about the community structure of methanotrophs in environmental samples than was previously available.
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Alphaproteobacteria/genética , Ecosistema , Microbiología Ambiental , Methylococcaceae/genética , Sondas de Oligonucleótidos/genética , ARN Ribosómico 16S/genética , Alphaproteobacteria/clasificación , Genes de ARNr , Metano/metabolismo , Methylococcaceae/clasificación , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Especificidad de la EspecieRESUMEN
A total of 25 young monkeys (Macaca mulatta) were trained with the Psychomotor Test System, a package of software tasks and computer hardware developed for spaceflight research with nonhuman primates. Two flight monkeys and two control monkeys were selected from this pool and performed a psychomotor task before and after the Bion 11 flight or a ground-control period. Monkeys from both groups showed significant disruption in performance after the 14-day flight or simulation (plus one anesthetized day of biopsies and other tests), and this disruption appeared to be magnified for the flight animal.
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Adaptación Psicológica , Desempeño Psicomotor/fisiología , Tiempo de Reacción/fisiología , Vuelo Espacial , Ingravidez , Animales , Conducta Animal , Masculino , Programas Informáticos , Interfaz Usuario-Computador , Juegos de VideoRESUMEN
NH4+ inhibition kinetics for CH4 oxidation were examined at near-atmospheric CH4 concentrations in three upland forest soils. Whether NH4+-independent salt effects could be neutralized by adding nonammoniacal salts to control samples in lieu of deionized water was also investigated. Because the levels of exchangeable endogenous NH4+ were very low in the three soils, desorption of endogenous NH4+ was not a significant factor in this study. The Km(app) values for water-treated controls were 9.8, 22, and 57 nM for temperate pine, temperate hardwood, and birch taiga soils, respectively. At CH4 concentrations of =15 &mgr;l liter-1, oxidation followed first-order kinetics in the fine-textured taiga soil, whereas the coarse-textured temperate soils exhibited Michaelis-Menten kinetics. Compared to water controls, the Km(app) values in the temperate soils increased in the presence of NH4+ salts, whereas the Vmax(app) values decreased substantially, indicating that there was a mixture of competitive and noncompetitive inhibition mechanisms for whole NH4+ salts. Compared to the corresponding K+ salt controls, the Km(app) values for NH4+ salts increased substantially, whereas the Vmax(app) values remained virtually unchanged, indicating that NH4+ acted by competitive inhibition. Nonammoniacal salts caused inhibition to increase with increasing CH4 concentrations in all three soils. In the birch taiga soil, this trend occurred with both NH4+ and K+ salts, and the slope of the increase was not affected by the addition of NH4+. Hence, the increase in inhibition resulted from an NH4+-independent mechanism. These results show that NH4+ inhibition of atmospheric CH4 oxidation resulted from enzymatic substrate competition and that additional inhibition that was not competitive resulted from a general salt effect that was independent of NH4+.
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Four rhesus monkeys (Macaca mulatta) were tested on joystick-based computer tasks in which they could choose to be reinforced either with pellets-only or with pellets + video. A variety of videotapes were used to reinforce task performance. The monkeys significantly preferred to be rewarded with a pellet and 10 s of a blank screen than a pellet plus 10 s of videotape. When they did choose to see videotaped images, however, they were significantly more likely to view video of themselves than video of their roommate or of unfamiliar conspecifics. These data support earlier findings of individual differences in preference for video reinforcement, and have clear implications for the study of face-recognition and self-recognition by nonhuman primates.
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Conducta de Elección , Motivación , Refuerzo en Psicología , Recompensa , Grabación de Cinta de Video , Alimentación Animal , Animales , Concienciación , Macaca mulatta , Masculino , Desempeño Psicomotor , Análisis y Desempeño de Tareas , Percepción VisualRESUMEN
Four experiments were conducted to assess whether or not rhesus macaques (Macaca mulatta) could represent the unperceived movements of a stimulus. Subjects were tested on 2 computerized tasks, HOLE (monkeys) and LASER (humans and monkeys), in which subjects needed to chase or shoot at, respectively, a moving target that either remained visible or became invisible for a portion of its path of movement. Response patterns were analyzed and compared between target-visible and target-invisible conditions. Results of Experiments 1, 2, and 3 demonstrated that the monkeys are capable of extrapolating movement. That this extrapolation involved internal representation of the target's invisible movement was suggested but not confirmed. Experiment 4, however, demonstrated that the monkeys are capable of representing the invisible displacements of a stimulus.
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Aprendizaje Discriminativo , Macaca mulatta/psicología , Percepción de Movimiento , Orientación , Desempeño Psicomotor , Animales , Atención , Humanos , Masculino , Enmascaramiento Perceptual , Solución de ProblemasRESUMEN
Game-like computer tasks offer many benefits for psychological research. In this paper, the usefulness of such tasks to bridge population differences (e.g., age, intelligence, species) is discussed and illustrated. A task called ALVIN was used to assess humans' and monkeys' working memory for sequences of colors with or without tones. Humans repeated longer lists than did the monkeys, and only humans benefited when the visual stimuli were accompanied by auditory cues. However, the monkeys did recall sequences at levels comparable to those reported elsewhere for children. Comparison of similarities and differences between the species is possible because the two groups were tested with exactly the same game-like paradigm.